BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0031.p (437 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775256.1| major facilitator superfamily permease [Herb... 285 1e-74 ref|ZP_03541210.1| major facilitator superfamily MFS_1 [Comamona... 240 4e-61 ref|YP_003280191.1| major facilitator superfamily protein [Comam... 238 1e-60 ref|ZP_07047285.1| major facilitator superfamily MFS_1 [Comamona... 237 3e-60 ref|YP_001562475.1| major facilitator transporter [Delftia acido... 234 2e-59 ref|YP_004490411.1| major facilitator superfamily protein [Delft... 234 2e-59 ref|YP_004753471.1| major facilitator superfamily protein [Colli... 232 1e-58 ref|YP_001898097.1| major facilitator superfamily protein [Ralst... 232 1e-58 ref|YP_002980475.1| major facilitator superfamily protein [Ralst... 229 6e-58 ref|YP_003979197.1| major facilitator protein [Achromobacter xyl... 228 1e-57 >ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ63348.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 285 bits (728), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT Sbjct: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 Query: 121 SFYILRFLLGIFEAGFFP 138 SFYILRFLLGIFEAGFFP Sbjct: 121 SFYILRFLLGIFEAGFFP 138 >ref|ZP_03541210.1| major facilitator superfamily MFS_1 [Comamonas testosteroni KF-1] gb|EED65496.1| major facilitator superfamily MFS_1 [Comamonas testosteroni KF-1] Length = 439 Score = 240 bits (612), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 116/138 (84%), Positives = 125/138 (90%), Gaps = 3/138 (2%) Query: 4 SPSAAATP-AAHASAAA--PLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 SP++A P A HA A A ++ TYRKI WRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 3 SPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 62 Query: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWG+ASMAMAYVTTPT Sbjct: 63 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGVASMAMAYVTTPT 122 Query: 121 SFYILRFLLGIFEAGFFP 138 FY+LRF+LG+ EAGFFP Sbjct: 123 MFYVLRFILGVVEAGFFP 140 >ref|YP_003280191.1| major facilitator superfamily protein [Comamonas testosteroni CNB-2] gb|ACY34895.1| major facilitator superfamily MFS_1 [Comamonas testosteroni CNB-2] Length = 439 Score = 238 bits (608), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 116/138 (84%), Positives = 124/138 (89%), Gaps = 3/138 (2%) Query: 4 SPSAAATP-AAHASAAA--PLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 SP++A P A HA A A ++ TYRKI WRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 3 SPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 62 Query: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWG ASMAMAYVTTPT Sbjct: 63 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGAASMAMAYVTTPT 122 Query: 121 SFYILRFLLGIFEAGFFP 138 FY+LRF+LG+ EAGFFP Sbjct: 123 MFYVLRFILGVVEAGFFP 140 >ref|ZP_07047285.1| major facilitator superfamily MFS_1 [Comamonas testosteroni S44] gb|EFI59086.1| major facilitator superfamily MFS_1 [Comamonas testosteroni S44] Length = 439 Score = 237 bits (604), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 115/138 (83%), Positives = 124/138 (89%), Gaps = 3/138 (2%) Query: 4 SPSAAATP-AAHASAAA--PLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 SP++A P A HA A A ++ TYRKI WRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 3 SPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 62 Query: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLE+IGARKTLARITILWG ASMAMAYVTTPT Sbjct: 63 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLERIGARKTLARITILWGAASMAMAYVTTPT 122 Query: 121 SFYILRFLLGIFEAGFFP 138 FY+LRF+LG+ EAGFFP Sbjct: 123 MFYVLRFILGVVEAGFFP 140 >ref|YP_001562475.1| major facilitator transporter [Delftia acidovorans SPH-1] gb|ABX34090.1| major facilitator superfamily MFS_1 [Delftia acidovorans SPH-1] Length = 439 Score = 234 bits (597), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 114/140 (81%), Positives = 124/140 (88%), Gaps = 2/140 (1%) Query: 1 MSYSPSAAATPAAHASAAAP--LEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQML 58 MS SAA +PA A A+P ++ TYRKIAWRLIPFLV LF+LAWIDRVNVGFAKLQML Sbjct: 1 MSSPASAAHSPAHGAGQASPPDMDATYRKIAWRLIPFLVLLFILAWIDRVNVGFAKLQML 60 Query: 59 QDLQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTT 118 DLQFSEA+YGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWG ASMAMAYVTT Sbjct: 61 DDLQFSEAIYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGAASMAMAYVTT 120 Query: 119 PTSFYILRFLLGIFEAGFFP 138 PT FY+LRF+LG+ EAGFFP Sbjct: 121 PTMFYVLRFILGVVEAGFFP 140 >ref|YP_004490411.1| major facilitator superfamily protein [Delftia sp. Cs1-4] gb|AEF92056.1| major facilitator superfamily MFS_1 [Delftia sp. Cs1-4] Length = 439 Score = 234 bits (597), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 114/140 (81%), Positives = 124/140 (88%), Gaps = 2/140 (1%) Query: 1 MSYSPSAAATPAAHASAAAP--LEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQML 58 MS SAA +PA A A+P ++ TYRKIAWRLIPFLV LF+LAWIDRVNVGFAKLQML Sbjct: 1 MSSPASAAHSPAHGAGQASPPDMDATYRKIAWRLIPFLVLLFILAWIDRVNVGFAKLQML 60 Query: 59 QDLQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTT 118 DLQFSEA+YGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWG ASMAMAYVTT Sbjct: 61 DDLQFSEAIYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGAASMAMAYVTT 120 Query: 119 PTSFYILRFLLGIFEAGFFP 138 PT FY+LRF+LG+ EAGFFP Sbjct: 121 PTMFYVLRFILGVVEAGFFP 140 >ref|YP_004753471.1| major facilitator superfamily protein [Collimonas fungivorans Ter331] gb|AEK62648.1| major facilitator superfamily MFS_1 [Collimonas fungivorans Ter331] Length = 428 Score = 232 bits (591), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 106/118 (89%), Positives = 112/118 (94%) Query: 21 LEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYF 80 LE TY+KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYF Sbjct: 12 LEATYKKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYF 71 Query: 81 IFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 +FEVPSNLLLEKIGARKTLARITILWGL SMAM YV TP +FY++RF LG+FEAGFFP Sbjct: 72 LFEVPSNLLLEKIGARKTLARITILWGLTSMAMVYVKTPATFYLIRFFLGVFEAGFFP 129 >ref|YP_001898097.1| major facilitator superfamily protein [Ralstonia pickettii 12J] gb|ACD25665.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12J] Length = 440 Score = 232 bits (591), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 106/119 (89%), Positives = 114/119 (95%) Query: 20 PLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGY 79 L+VTYR+IA+RL+PFLV LF+LAWIDRVNVGFAKL MLQDLQFSEAVYGLGAGIFFIGY Sbjct: 23 ELDVTYRRIAYRLVPFLVVLFILAWIDRVNVGFAKLSMLQDLQFSEAVYGLGAGIFFIGY 82 Query: 80 FIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 F+FEVPSNLLLEKIGARKTLARITILWGLASMAM YV TPTSFY++RFLLGIFEAGFFP Sbjct: 83 FLFEVPSNLLLEKIGARKTLARITILWGLASMAMIYVKTPTSFYVMRFLLGIFEAGFFP 141 >ref|YP_002980475.1| major facilitator superfamily protein [Ralstonia pickettii 12D] gb|ACS61803.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12D] Length = 440 Score = 229 bits (585), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 106/119 (89%), Positives = 113/119 (94%) Query: 20 PLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGY 79 LE TYR+IA+RL+PFLV LF+LAWIDRVNVGFAKL MLQDLQFSEAVYGLGAGIFFIGY Sbjct: 23 ELEATYRRIAYRLVPFLVVLFILAWIDRVNVGFAKLSMLQDLQFSEAVYGLGAGIFFIGY 82 Query: 80 FIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 F+FEVPSNLLLEKIGARKTLARITILWGLASMAM YV TPTSFY++RFLLGIFEAGFFP Sbjct: 83 FLFEVPSNLLLEKIGARKTLARITILWGLASMAMIYVKTPTSFYVMRFLLGIFEAGFFP 141 >ref|YP_003979197.1| major facilitator protein [Achromobacter xylosoxidans A8] gb|ADP16482.1| major facilitator transporter 3 [Achromobacter xylosoxidans A8] Length = 441 Score = 228 bits (582), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 108/128 (84%), Positives = 119/128 (92%) Query: 11 PAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGL 70 P ASAAA + +RK+AWRLIPFL+FLF+LAWIDRVNVGFAKLQMLQDL+FSEAVYGL Sbjct: 13 PQDAASAAADPGLMHRKVAWRLIPFLIFLFLLAWIDRVNVGFAKLQMLQDLRFSEAVYGL 72 Query: 71 GAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLG 130 GAGIFFIGYFIFEVPSNLLL++IGARKTLARITI+WGL SMAMA+VTTPTSFY+LRFLLG Sbjct: 73 GAGIFFIGYFIFEVPSNLLLQRIGARKTLARITIMWGLTSMAMAFVTTPTSFYVLRFLLG 132 Query: 131 IFEAGFFP 138 FEAGFFP Sbjct: 133 AFEAGFFP 140