BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0187.p (260 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776396.1| permease [Herbaspirillum seropedicae SmR1] >... 425 e-117 ref|YP_002499451.1| hypothetical protein Mnod_4272 [Methylobacte... 246 2e-63 ref|YP_001895773.1| hypothetical protein Bphyt_2148 [Burkholderi... 244 7e-63 ref|ZP_07777498.1| membrane protein [Pseudomonas fluorescens WH6... 244 1e-62 ref|YP_004753795.1| membrane protein [Collimonas fungivorans Ter... 238 9e-61 ref|YP_001927507.1| hypothetical protein Mpop_4876 [Methylobacte... 232 4e-59 ref|YP_002874688.1| hypothetical protein PFLU5185 [Pseudomonas f... 232 5e-59 ref|YP_001269829.1| hypothetical protein Pput_4525 [Pseudomonas ... 232 5e-59 gb|ADR61933.1| Hypothetical protein, conserved [Pseudomonas puti... 229 3e-58 ref|NP_746772.1| hypothetical protein PP_4663 [Pseudomonas putid... 229 3e-58 >ref|YP_003776396.1| permease [Herbaspirillum seropedicae SmR1] gb|ADJ64488.1| permease protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 425 bits (1093), Expect = e-117, Method: Compositional matrix adjust. Identities = 254/260 (97%), Positives = 255/260 (98%) Query: 1 MSSFLSSIDMSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVP 60 MSSFLSSIDMSATVL ASL VF+LAGFVKG IGLGLPTVAVGLLSLMMPPVQAA LLIVP Sbjct: 1 MSSFLSSIDMSATVLPASLPVFMLAGFVKGSIGLGLPTVAVGLLSLMMPPVQAAPLLIVP 60 Query: 61 SMVTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYA 120 SMVTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYA Sbjct: 61 SMVTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYA 120 Query: 121 LAGLASWTPSVSPAQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALG 180 LAGLASWTPSVSPAQEQWWSPAVGL TGLVTAATGVFVIPAVPYLQALGLPREQLIQALG Sbjct: 121 LAGLASWTPSVSPAQEQWWSPAVGLATGLVTAATGVFVIPAVPYLQALGLPREQLIQALG 180 Query: 181 LSFTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFI 240 LSFTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFI Sbjct: 181 LSFTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFI 240 Query: 241 GLLLLGLHLASALLRQGGAA 260 GLLLLGLHLASALLRQGGAA Sbjct: 241 GLLLLGLHLASALLRQGGAA 260 >ref|YP_002499451.1| hypothetical protein Mnod_4272 [Methylobacterium nodulans ORS 2060] gb|ACL59148.1| protein of unknown function DUF81 [Methylobacterium nodulans ORS 2060] Length = 251 Score = 246 bits (629), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 150/252 (59%), Positives = 175/252 (69%), Gaps = 6/252 (2%) Query: 1 MSSFLSSIDMSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVP 60 M+S L + ++ T L FLLAGFVKGVIGLGLPTVA+GLLSL+M P QAAALLIVP Sbjct: 1 MTSALDPLPIAITAL-----TFLLAGFVKGVIGLGLPTVAMGLLSLVMAPAQAAALLIVP 55 Query: 61 SMVTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYA 120 S VTN WQL G L RLW ++ G+V+GTLAG F+ +A I LGLAL+ YA Sbjct: 56 SFVTNVWQLAAGPRFGTLAHRLWPMMVGVVVGTLAGAGFLQGSHAGQAAIALGLALMAYA 115 Query: 121 LAGLASWTPSVSP-AQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQAL 179 + GL + ++ P AQ W P +G TGLVTAATGVFVIPAVPYL ALGL +++LIQAL Sbjct: 116 VLGLTAVRFALPPEAQGGWMGPLIGALTGLVTAATGVFVIPAVPYLGALGLAKDELIQAL 175 Query: 180 GLSFTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFF 239 GLSFTVSTLALA L G F G AS L PAL+GM++G VRGRV FRRCFF Sbjct: 176 GLSFTVSTLALAAVLAGSGVFEPGTAGASAFALGPALVGMAMGGWVRGRVSEPVFRRCFF 235 Query: 240 IGLLLLGLHLAS 251 +GLL LG HLAS Sbjct: 236 LGLLALGTHLAS 247 >ref|YP_001895773.1| hypothetical protein Bphyt_2148 [Burkholderia phytofirmans PsJN] gb|ACD16549.1| protein of unknown function DUF81 [Burkholderia phytofirmans PsJN] Length = 243 Score = 244 bits (624), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 140/240 (58%), Positives = 176/240 (73%), Gaps = 3/240 (1%) Query: 10 MSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNGWQL 69 M A V+AA+ F++AGFVKGV G+GLPTVA+G+L +M P QAAA+L++PS VTN WQL Sbjct: 1 MRAGVVAAT---FVVAGFVKGVTGMGLPTVAMGVLGTLMLPAQAAAMLLLPSFVTNVWQL 57 Query: 70 LTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTP 129 G ++RAL++RLW ++ GIV GT+A SF+ GG A++ LG AL LYA+AGL +W Sbjct: 58 FAGPSVRALLKRLWPMMFGIVAGTVAAASFIAGGGGEWAVVGLGAALTLYAVAGLLAWRF 117 Query: 130 SVSPAQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLA 189 SV E+W SPA+G TGLVT TGVFV+PAVPYLQ L L +E L+QALGLSFTVST+A Sbjct: 118 SVPARHERWLSPAIGAVTGLVTGGTGVFVVPAVPYLQGLELKKEDLVQALGLSFTVSTIA 177 Query: 190 LAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGLLLLGLHL 249 LA+ L + AF+ G SL ++PALLGM IG VRGR+ A TFRR FFI LL LG+ L Sbjct: 178 LAIGLTREHAFAAGDLGESLFAVVPALLGMWIGQHVRGRIGANTFRRWFFICLLGLGIQL 237 >ref|ZP_07777498.1| membrane protein [Pseudomonas fluorescens WH6] gb|EFQ61376.1| membrane protein [Pseudomonas fluorescens WH6] Length = 254 Score = 244 bits (622), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 148/250 (59%), Positives = 180/250 (72%), Gaps = 1/250 (0%) Query: 1 MSSFLSSI-DMSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIV 59 M++FL+ ++ T+ +A FLLAG VKGVIGLGLPTVA+GLL L M P QAAALLI+ Sbjct: 1 MNTFLTFYQNLGPTLTLLVIATFLLAGAVKGVIGLGLPTVAMGLLGLAMLPAQAAALLII 60 Query: 60 PSMVTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLY 119 PS VTN WQL GG L AL++RLW +L I LGT G ++ DGGR + LG AL++Y Sbjct: 61 PSTVTNLWQLAFGGHLSALLKRLWPMLLLIFLGTGLGTVWLGMDGGRWVVRALGGALLVY 120 Query: 120 ALAGLASWTPSVSPAQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQAL 179 AL+GL T V + E+W+ P GL TGL+T+ATGVFVIPAVPYLQALGL R+QL+QAL Sbjct: 121 ALSGLFMPTFKVKTSTERWFGPVCGLITGLITSATGVFVIPAVPYLQALGLNRDQLVQAL 180 Query: 180 GLSFTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFF 239 GLSFTVSTLALA L G GG ASL+ L+PALLGM +G +R R+ AV F+R FF Sbjct: 181 GLSFTVSTLALAAGLAWRGTLGGGEVTASLLALVPALLGMWLGQALRQRISAVLFKRIFF 240 Query: 240 IGLLLLGLHL 249 IG+ LLG HL Sbjct: 241 IGMALLGAHL 250 >ref|YP_004753795.1| membrane protein [Collimonas fungivorans Ter331] gb|AEK62972.1| membrane protein, putative [Collimonas fungivorans Ter331] Length = 268 Score = 238 bits (606), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 142/236 (60%), Positives = 170/236 (72%), Gaps = 2/236 (0%) Query: 15 LAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNGWQLLTGGA 74 L+A L F +AG VKGV G+GLPT+A+GLL + MPPV AA LLI+PS VTN WQLL G Sbjct: 28 LSAILLTFFIAGTVKGVTGMGLPTIAMGLLGITMPPVAAATLLILPSFVTNLWQLLAGPG 87 Query: 75 LRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTPSVSPA 134 AL RRLWT++AGI +GT+AG M D G I LG+ LV+YAL GLA+ SV Sbjct: 88 FLALARRLWTMMAGIAVGTIAGAWLMTNDSGGWTAIALGVVLVVYALFGLAARQLSVPTR 147 Query: 135 QEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLALAVNL 194 E+ SP VGL TGL+T TGVFVIPAVPY+QALGL +E L+QALGLSFTVST+ALA+ L Sbjct: 148 AERPASPLVGLVTGLITGGTGVFVIPAVPYIQALGLDKEDLVQALGLSFTVSTIALAIGL 207 Query: 195 GQGGAFS-GGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGLLLLGLHL 249 + GAF G +G +SL +LPALLGM +G VR RV TFRR FFI L++LGL L Sbjct: 208 QRQGAFELGDIGTSSLA-VLPALLGMWLGQHVRRRVSPATFRRWFFICLIVLGLQL 262 >ref|YP_001927507.1| hypothetical protein Mpop_4876 [Methylobacterium populi BJ001] gb|ACB82972.1| protein of unknown function DUF81 [Methylobacterium populi BJ001] Length = 250 Score = 232 bits (592), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 135/234 (57%), Positives = 163/234 (69%) Query: 7 SIDMSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNG 66 S + LA + + FLLAGFVKG++GLGLP VA+GLLSL+M PVQAAALL+VP+ VTN Sbjct: 2 SFSLDPLPLAVAASAFLLAGFVKGMVGLGLPPVAMGLLSLVMAPVQAAALLVVPAFVTNV 61 Query: 67 WQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLAS 126 WQL G L+RRLW +LA V+GTL ++ + G A + LGLAL+ YA+ GLA+ Sbjct: 62 WQLAAGPRFGTLLRRLWPMLAASVVGTLVAAGILHGNYGAEATVFLGLALIAYAVVGLAA 121 Query: 127 WTPSVSPAQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVS 186 V PA E+W P VG TGLVTAAT V P+ PYL ALGL ++ LIQALGLSFTVS Sbjct: 122 LRLHVPPAAERWLGPLVGAATGLVTAATAVSSFPSAPYLGALGLTKDDLIQALGLSFTVS 181 Query: 187 TLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFI 240 T+ALAV L GGA S V +SLV L+PALLGM +G VRGRV FRRCFF+ Sbjct: 182 TVALAVALAGGGALSLSVAGSSLVALIPALLGMVLGGWVRGRVSERVFRRCFFV 235 >ref|YP_002874688.1| hypothetical protein PFLU5185 [Pseudomonas fluorescens SBW25] emb|CAY52246.1| putative putative membrane protein [Pseudomonas fluorescens SBW25] Length = 261 Score = 232 bits (591), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 144/247 (58%), Positives = 174/247 (70%), Gaps = 2/247 (0%) Query: 3 SFLSSIDMSATVLAASLAVFLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSM 62 +F I + T+L + FLLAG VKGVIGLGLPTVA+GLL L M P QAAALLI+PS Sbjct: 12 AFYQHIGPALTLLV--IGTFLLAGTVKGVIGLGLPTVAMGLLGLAMLPAQAAALLIIPST 69 Query: 63 VTNGWQLLTGGALRALVRRLWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALA 122 VTN WQL GG L AL++RLW +L I LGT G ++ DGG + LG AL++YAL+ Sbjct: 70 VTNLWQLAFGGHLSALIKRLWPMLLLIFLGTGLGTLWLGMDGGGWVVHALGGALLMYALS 129 Query: 123 GLASWTPSVSPAQEQWWSPAVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLS 182 GL + V+P E+W P G TG++T+ATGVFVIPAVPYLQALGL R+QL+QALGLS Sbjct: 130 GLFLPSFKVAPHTERWLGPLCGGITGIITSATGVFVIPAVPYLQALGLSRDQLVQALGLS 189 Query: 183 FTVSTLALAVNLGQGGAFSGGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGL 242 FTVSTLALA L G GG ASL+ L+PALLGM +G VR R+ A+ F+R FFIG+ Sbjct: 190 FTVSTLALAAGLAWRGTLGGGELNASLLALVPALLGMWLGQVVRQRISALLFKRVFFIGM 249 Query: 243 LLLGLHL 249 LLG HL Sbjct: 250 ALLGGHL 256 >ref|YP_001269829.1| hypothetical protein Pput_4525 [Pseudomonas putida F1] gb|ABQ80645.1| protein of unknown function DUF81 [Pseudomonas putida F1] Length = 254 Score = 232 bits (591), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 139/228 (60%), Positives = 167/228 (73%) Query: 22 FLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNGWQLLTGGALRALVRR 81 FLLAG VKGVIGLGLPT+A+GLL L MPP QAAALLIVPS +TN WQL +GG LR L+ R Sbjct: 23 FLLAGAVKGVIGLGLPTIAMGLLGLAMPPAQAAALLIVPSTLTNLWQLASGGHLRGLLVR 82 Query: 82 LWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTPSVSPAQEQWWSP 141 L +LA I +GTL G +++ + G A LG AL++YAL GL V+P E+W P Sbjct: 83 LGPMLALIFVGTLLGSAWLGINSGPWAGHALGGALLVYALYGLVGPGLQVAPGHERWLGP 142 Query: 142 AVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLALAVNLGQGGAFS 201 GL TG+VTA+TGVFV+PAVPYLQ+LGL REQ+IQALGLSFTVSTLALA+ L A Sbjct: 143 LCGLVTGVVTASTGVFVMPAVPYLQSLGLSREQMIQALGLSFTVSTLALALGLAGQDALG 202 Query: 202 GGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGLLLLGLHL 249 G ASL++L PALLGM G +R R+ A+ F+RCFFIGL LG+HL Sbjct: 203 GQALGASLLMLAPALLGMLAGQWLRQRISALLFKRCFFIGLAALGVHL 250 >gb|ADR61933.1| Hypothetical protein, conserved [Pseudomonas putida BIRD-1] Length = 254 Score = 229 bits (585), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 138/228 (60%), Positives = 167/228 (73%) Query: 22 FLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNGWQLLTGGALRALVRR 81 FLLAG VKGVIGLGLPT+A+GLL L MPP QAAALLIVPS +TN WQL +GG LR L+ R Sbjct: 23 FLLAGAVKGVIGLGLPTIAMGLLGLAMPPAQAAALLIVPSTLTNLWQLASGGHLRGLLVR 82 Query: 82 LWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTPSVSPAQEQWWSP 141 L +LA I++GTL G +++ + G A LG AL++YAL G+ V+P E+W P Sbjct: 83 LGPMLALILVGTLLGSAWLGINTGPWAAHALGGALLVYALYGVVGPGLQVAPGHERWLGP 142 Query: 142 AVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLALAVNLGQGGAFS 201 GL TG+VTA+TGVFV+PAVPYLQ+LGL REQ+IQALGLSFTVSTLALA+ L A Sbjct: 143 LCGLVTGVVTASTGVFVMPAVPYLQSLGLSREQMIQALGLSFTVSTLALALGLAGQDALG 202 Query: 202 GGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGLLLLGLHL 249 G ASL++L PALLGM G +R R+ A+ F+RCFFIGL LG HL Sbjct: 203 GQALGASLLMLAPALLGMLAGQWLRQRISALLFKRCFFIGLAALGGHL 250 >ref|NP_746772.1| hypothetical protein PP_4663 [Pseudomonas putida KT2440] gb|AAN70236.1|AE016663_4 membrane protein, putative [Pseudomonas putida KT2440] Length = 254 Score = 229 bits (584), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 138/228 (60%), Positives = 166/228 (72%) Query: 22 FLLAGFVKGVIGLGLPTVAVGLLSLMMPPVQAAALLIVPSMVTNGWQLLTGGALRALVRR 81 FLLAG VKGVIGLGLPT+A+GLL L MPP QAAALLIVPS +TN WQL +GG LR L+ R Sbjct: 23 FLLAGAVKGVIGLGLPTIAMGLLGLAMPPAQAAALLIVPSTLTNLWQLASGGHLRGLLVR 82 Query: 82 LWTLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTPSVSPAQEQWWSP 141 L +LA I +GTL G +++ + G A LG AL++YAL GL ++P E+W P Sbjct: 83 LGPMLALIFVGTLLGSAWLGINSGPWAAHALGGALLVYALYGLVGPGLQLAPGHERWLGP 142 Query: 142 AVGLTTGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLALAVNLGQGGAFS 201 GL TG+VTA+TGVFV+PAVPYLQ+LGL REQ+IQALGLSFTVSTLALA+ L A Sbjct: 143 LCGLVTGVVTASTGVFVMPAVPYLQSLGLSREQMIQALGLSFTVSTLALALGLAGQDALG 202 Query: 202 GGVGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFIGLLLLGLHL 249 G ASL++L PALLGM G +R R+ A+ F+RCFFIGL LG HL Sbjct: 203 GQALGASLLMLAPALLGMLAGQWLRQRISALLFKRCFFIGLAALGGHL 250