BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0066.p (254 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776204.1| transmembrane permease [Herbaspirillum serop... 496 e-138 ref|YP_003882273.1| hypothetical protein Dda3937_00781 [Dickeya ... 233 1e-59 ref|YP_585970.1| hypothetical protein Rmet_3831 [Cupriavidus met... 229 2e-58 ref|ZP_07673878.1| membrane protein [Ralstonia sp. 5_7_47FAA] >g... 227 1e-57 ref|YP_003749150.1| hypothetical protein RPSI07_mp0142 [Ralstoni... 227 1e-57 ref|YP_299298.1| hypothetical protein Reut_B5106 [Ralstonia eutr... 225 5e-57 ref|YP_002008784.1| hypothetical protein RALTA_B2155 [Cupriavidu... 224 7e-57 ref|YP_001892438.1| protein of unknown function DUF81 [Ralstonia... 223 2e-56 ref|YP_003332915.1| hypothetical protein Dd586_1331 [Dickeya dad... 219 2e-55 ref|ZP_03697157.1| protein of unknown function DUF81 [Lutiella n... 219 2e-55 >ref|YP_003776204.1| transmembrane permease [Herbaspirillum seropedicae SmR1] gb|ADJ64296.1| transmembrane permease protein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 496 bits (1276), Expect = e-138, Method: Compositional matrix adjust. Identities = 254/254 (100%), Positives = 254/254 (100%) Query: 1 MTASLFFSGVVNFLLGGLLGAMGGLFGIGGGLIAIPVLGYLYGMDQQLAQGTSLVMIVPN 60 MTASLFFSGVVNFLLGGLLGAMGGLFGIGGGLIAIPVLGYLYGMDQQLAQGTSLVMIVPN Sbjct: 1 MTASLFFSGVVNFLLGGLLGAMGGLFGIGGGLIAIPVLGYLYGMDQQLAQGTSLVMIVPN 60 Query: 61 VVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSARLATAIDARSLHFCFAVFMAVLALYY 120 VVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSARLATAIDARSLHFCFAVFMAVLALYY Sbjct: 61 VVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSARLATAIDARSLHFCFAVFMAVLALYY 120 Query: 121 LWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQ 180 LWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQ Sbjct: 121 LWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQ 180 Query: 181 GLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFC 240 GLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFC Sbjct: 181 GLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFC 240 Query: 241 GALLLLSVWMMVGG 254 GALLLLSVWMMVGG Sbjct: 241 GALLLLSVWMMVGG 254 >ref|YP_003882273.1| hypothetical protein Dda3937_00781 [Dickeya dadantii 3937] gb|ADM97716.1| putative membrane protein [Dickeya dadantii 3937] Length = 254 Score = 233 bits (595), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 132/252 (52%), Positives = 178/252 (70%) Query: 1 MTASLFFSGVVNFLLGGLLGAMGGLFGIGGGLIAIPVLGYLYGMDQQLAQGTSLVMIVPN 60 MT+ L + V+ +LG LG GG+ GIGGGLIAIPVLG L+GM+Q LAQGT+L+MI PN Sbjct: 1 MTSLLMTNMVLCLILGMGLGVCGGMLGIGGGLIAIPVLGMLFGMNQHLAQGTALIMITPN 60 Query: 61 VVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSARLATAIDARSLHFCFAVFMAVLALYY 120 V+I F RY+QRNKI+ M+CA A V+ YF+A +A+AI +L FA+F+ VLA YY Sbjct: 61 VLIGFLRYRQRNKIDTRMTLMLCAFATVSAYFAAHIASAIQVDNLQQAFAIFLLVLATYY 120 Query: 121 LWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQ 180 +W + + VL +R++P +G+++G +G F+VGG +V VP L FG TQT AQ Sbjct: 121 IWQWINSRRSRTPTSVLSKRYLPALGVASGFMSGIFTVGGGLVVVPALVTLFGFTQTQAQ 180 Query: 181 GLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFC 240 G+ LA+V PG L AL +Y AG+VDWA GIP+A+GG+LS+SWGVALAH LP LR+ FC Sbjct: 181 GIALALVVPGALAALVSYTQAGYVDWATGIPLAIGGILSVSWGVALAHKLPVVALRLAFC 240 Query: 241 GALLLLSVWMMV 252 L+ ++V M+V Sbjct: 241 LVLVGVAVVMLV 252 >ref|YP_585970.1| hypothetical protein Rmet_3831 [Cupriavidus metallidurans CH34] gb|ABF10701.1| conserved hypothetical protein [Cupriavidus metallidurans CH34] Length = 277 Score = 229 bits (585), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 133/221 (60%), Positives = 159/221 (71%) Query: 34 AIPVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFS 93 AIP LG LYGMDQQLAQGTSLVMI PNV+I FW+Y++R IE TA ++ AV+ATY S Sbjct: 57 AIPALGLLYGMDQQLAQGTSLVMIAPNVLIGFWQYRKRADIELKTAIVLGLSAVLATYAS 116 Query: 94 ARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASA 153 A+ AT+IDA L FAVFM LALY+LW LL L WIP VG+ GA + Sbjct: 117 AKFATSIDAALLRRIFAVFMIGLALYFLWRLLPGRATTQPQARLSPSWIPAVGVVGGAFS 176 Query: 154 GFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMA 213 GFFSVGG +VA P L FGM Q AAQGL LA+VTPG +VAL+TYA+AGHVDW GIP++ Sbjct: 177 GFFSVGGGVVAAPALVGLFGMRQAAAQGLALALVTPGAVVALFTYANAGHVDWYSGIPLS 236 Query: 214 LGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMVGG 254 LGG+L+ISWGVALAH +PER+LR F L+ +V M+V G Sbjct: 237 LGGMLTISWGVALAHRMPERKLRAAFALCLIATAVVMLVRG 277 >ref|ZP_07673878.1| membrane protein [Ralstonia sp. 5_7_47FAA] gb|EFP67806.1| membrane protein [Ralstonia sp. 5_7_47FAA] Length = 260 Score = 227 bits (579), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 120/224 (53%), Positives = 156/224 (69%), Gaps = 4/224 (1%) Query: 33 IAIPVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYF 92 IAIPVLGYLYGMDQ LAQGT+LVMI PNV+I FWRY QR+ + + A++C ++ ATY Sbjct: 33 IAIPVLGYLYGMDQHLAQGTALVMIAPNVLIGFWRYHQRHPVHLRSVALICVFSMAATYV 92 Query: 93 SARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPA----VVRPVVLGRRWIPFVGLS 148 +AR A +DA LH FAVF+ VLALY+ L + A P + +P +G++ Sbjct: 93 AARFAAGLDAHLLHTAFAVFLIVLALYFASQLKEKPHAGDTAHAAPRAMPAAALPLMGVA 152 Query: 149 AGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAV 208 +GA +G F+VGG +V VP L FFGM QT AQG+ LA+V PG+L+AL TYAHAGHVDW Sbjct: 153 SGAMSGIFTVGGGLVVVPALVTFFGMPQTRAQGMALALVVPGSLIALATYAHAGHVDWGT 212 Query: 209 GIPMALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMV 252 GIP+A GG+ S+SWGVALA+ RRLR+ FC LL ++ M++ Sbjct: 213 GIPLAAGGIASVSWGVALAYRFSARRLRLAFCTVLLGTALMMLL 256 >ref|YP_003749150.1| hypothetical protein RPSI07_mp0142 [Ralstonia solanacearum PSI07] emb|CBJ34501.1| conserved membrane protein of unknown function, DUF81 [Ralstonia solanacearum PSI07] Length = 262 Score = 227 bits (578), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 119/230 (51%), Positives = 157/230 (68%), Gaps = 8/230 (3%) Query: 33 IAIPVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYF 92 IAIP+LGYLYGMDQ LAQGT+LVMI PNV+I F RY QR+ + + A +CA A+V+TY Sbjct: 33 IAIPILGYLYGMDQHLAQGTALVMIAPNVLIGFLRYHQRHPVPLRSVATLCAFAMVSTYV 92 Query: 93 SARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRS--------PAVVRPVVLGRRWIPF 144 +AR A +DA LH FA F+ VLA+Y+ L ++ PA P + +P Sbjct: 93 AARFAAGLDADHLHTAFAWFLIVLAVYFASQLRDKAHPDPAAGHPAPEAPRAVPPAALPL 152 Query: 145 VGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHV 204 +G+++G +G F+VGG +V VP L FGM+QT AQG+ LA+V PG+L+AL TYA AGHV Sbjct: 153 LGIASGGMSGIFTVGGGLVVVPALVTLFGMSQTRAQGMALALVVPGSLIALATYAQAGHV 212 Query: 205 DWAVGIPMALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMVGG 254 W GIP+ALGG+ S+SWGVALAH RLR++FC L+ +V M+VGG Sbjct: 213 SWGTGIPLALGGMASVSWGVALAHRFSPLRLRLVFCAVLVGAAVAMLVGG 262 >ref|YP_299298.1| hypothetical protein Reut_B5106 [Ralstonia eutropha JMP134] gb|AAZ64454.1| Protein of unknown function DUF81 [Ralstonia eutropha JMP134] Length = 255 Score = 225 bits (574), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 132/222 (59%), Positives = 159/222 (71%), Gaps = 1/222 (0%) Query: 34 AIPVLGYLYGMD-QQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYF 92 AIP LG L+GMD QQLAQGT+LVMI PNV+I FW+Y++R I A M+ AV+ATY Sbjct: 34 AIPALGLLFGMDDQQLAQGTALVMIAPNVLIGFWQYRKRADIALRMALMLGLSAVLATYL 93 Query: 93 SARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPAVVRPVVLGRRWIPFVGLSAGAS 152 SARLAT++DA+ L FA FM LALY+LW LL + + RWIP VG+ GA Sbjct: 94 SARLATSMDAQLLRRIFAGFMIGLALYFLWRLLPGRAITAQHARVSPRWIPLVGVVGGAF 153 Query: 153 AGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAVGIPM 212 +GFFSVGG +VA P L FGM Q AAQGL LA+VTPG +VAL TYAHAGHVDW GIP+ Sbjct: 154 SGFFSVGGGVVAAPALVGLFGMRQAAAQGLALALVTPGAVVALATYAHAGHVDWVSGIPL 213 Query: 213 ALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMVGG 254 +LGG+L+ISWGVALAH +PERRLR LF L+ + M+V G Sbjct: 214 SLGGMLTISWGVALAHRMPERRLRALFALCLIATAAVMLVRG 255 >ref|YP_002008784.1| hypothetical protein RALTA_B2155 [Cupriavidus taiwanensis LMG 19424] emb|CAQ72732.1| conserved hypothetical protein; putative membrane protein [Cupriavidus taiwanensis LMG 19424] Length = 254 Score = 224 bits (572), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 129/221 (58%), Positives = 159/221 (71%) Query: 34 AIPVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFS 93 AIP LG L+GMDQQLAQGT+LVMI PNV+I FW+Y++R I TA ++ AV+AT+ S Sbjct: 34 AIPALGLLFGMDQQLAQGTALVMIAPNVLIGFWQYRKRADIALRTAVVLGLSAVLATWLS 93 Query: 94 ARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASA 153 AR ATA+DA+ L FA+FM +ALY+LW LL + RWIP VG+ G + Sbjct: 94 ARTATAMDAQLLRRIFALFMIGMALYFLWRLLPGRAVTAPQARVSARWIPMVGVVGGLFS 153 Query: 154 GFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMA 213 GFFSVGG +VA P L FG+ Q AAQGL LA+VTPG +VAL TYAHAG VDWA GIP++ Sbjct: 154 GFFSVGGGVVAAPALVGLFGLRQAAAQGLALALVTPGAVVALATYAHAGQVDWASGIPLS 213 Query: 214 LGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMVGG 254 LGG+L+ISWGVALAH +PERRLR LF L+ +V M+V G Sbjct: 214 LGGMLTISWGVALAHRMPERRLRALFAVCLIATAVVMLVRG 254 >ref|YP_001892438.1| protein of unknown function DUF81 [Ralstonia pickettii 12J] ref|YP_002983940.1| hypothetical protein Rpic12D_4018 [Ralstonia pickettii 12D] gb|ACD29011.1| protein of unknown function DUF81 [Ralstonia pickettii 12J] gb|ACS65268.1| protein of unknown function DUF81 [Ralstonia pickettii 12D] Length = 260 Score = 223 bits (568), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 114/221 (51%), Positives = 152/221 (68%), Gaps = 4/221 (1%) Query: 36 PVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSAR 95 P+LGYLYGMDQ LAQGT+LVMI PNV+I FWRY QR+ + + A++C ++ ATY +AR Sbjct: 36 PLLGYLYGMDQHLAQGTALVMIAPNVLIGFWRYHQRHPVHVRSVALICVFSMGATYVAAR 95 Query: 96 LATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPAV----VRPVVLGRRWIPFVGLSAGA 151 A +DA LH FAVF+ VLA+Y+ L + A P + +P +G+++GA Sbjct: 96 FAAGLDANLLHTAFAVFLIVLAVYFASQLKEKPNAAGAAHAVPHTMPAAALPLMGIASGA 155 Query: 152 SAGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAVGIP 211 +G F+VGG +V V L FFGM QT AQG+ LA+V PG+L+AL YAHAGHVDW GIP Sbjct: 156 MSGIFTVGGGLVVVSALVTFFGMPQTRAQGMALALVVPGSLIALAAYAHAGHVDWGTGIP 215 Query: 212 MALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMV 252 +A GGV+S+SWGVALA+ RRLR+ FC LL ++ M++ Sbjct: 216 LAAGGVVSVSWGVALAYRFSARRLRLAFCAVLLGTALMMLL 256 >ref|YP_003332915.1| hypothetical protein Dd586_1331 [Dickeya dadantii Ech586] gb|ACZ76210.1| protein of unknown function DUF81 [Dickeya dadantii Ech586] Length = 254 Score = 219 bits (559), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 132/253 (52%), Positives = 174/253 (68%) Query: 1 MTASLFFSGVVNFLLGGLLGAMGGLFGIGGGLIAIPVLGYLYGMDQQLAQGTSLVMIVPN 60 MT+ L V +LG LG GG+ GIGGGLIAIPVLG L+GM+Q LAQGT+L+MI PN Sbjct: 1 MTSVLITQVAVCLILGMGLGVCGGMLGIGGGLIAIPVLGMLFGMNQHLAQGTALIMITPN 60 Query: 61 VVIAFWRYKQRNKIEWSTAAMMCAPAVVATYFSARLATAIDARSLHFCFAVFMAVLALYY 120 V+I F RY+QRNKI+ M+CA A V+ Y +A +A AI +L FA+F+ VLA YY Sbjct: 61 VLIGFLRYRQRNKIDTRMTLMLCAFATVSAYIAAHMAAAIQVDNLQQAFAIFLLVLAAYY 120 Query: 121 LWTLLRRSPAVVRPVVLGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQ 180 +W + + VL R++P +G+++G +G F+VGG +V VP L FG TQT AQ Sbjct: 121 IWQWVNSQRSRTPTSVLSARYLPVLGVASGFMSGIFTVGGGLVVVPALVTLFGFTQTQAQ 180 Query: 181 GLGLAMVTPGTLVALWTYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFC 240 G+ LA+V PG L AL +Y AG+VDWA GIP+A+GG+LS+SWGVALAH LP LR+ FC Sbjct: 181 GIALALVVPGALAALVSYTQAGNVDWATGIPLAIGGILSVSWGVALAHKLPVVALRLTFC 240 Query: 241 GALLLLSVWMMVG 253 L+ ++V M+VG Sbjct: 241 LVLVGVAVVMLVG 253 >ref|ZP_03697157.1| protein of unknown function DUF81 [Lutiella nitroferrum 2002] gb|EEG09643.1| protein of unknown function DUF81 [Lutiella nitroferrum 2002] Length = 254 Score = 219 bits (559), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 117/220 (53%), Positives = 151/220 (68%), Gaps = 2/220 (0%) Query: 33 IAIPVLGYLYGMDQQLAQGTSLVMIVPNVVIAFWRYKQRNKIEWSTAAMMCAPAVVATYF 92 IAIPVLG LYGM+Q LAQGT+LVMI PNV+I F RY+QRN I+ M A V++Y Sbjct: 33 IAIPVLGLLYGMNQHLAQGTTLVMITPNVLIGFLRYRQRNDIDTRAVLTMGLTATVSSYI 92 Query: 93 SARLATAIDARSLHFCFAVFMAVLALYYLWTLLRRSPAVVRP-VVLGRRWIPFVGLSAGA 151 +AR A +++A SL FA+F+ +LA Y+ W L R RP V+L + P +G+ +GA Sbjct: 93 AARFAASLNAHSLQHAFALFLVLLASYFAW-LSRSDRHDRRPAVMLPAGFHPLLGVVSGA 151 Query: 152 SAGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALWTYAHAGHVDWAVGIP 211 +G F+VGG +V VP L FG TQT AQG+ LA+V PG L AL +Y AG+VDW VGIP Sbjct: 152 MSGIFTVGGGLVVVPALVTLFGFTQTQAQGMALALVVPGALAALLSYTQAGNVDWGVGIP 211 Query: 212 MALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMM 251 +A+GGVLS+SWGVALAH L RLR+ FC L+ +V M+ Sbjct: 212 LAIGGVLSVSWGVALAHKLSAARLRLAFCAVLVGSAVMML 251