BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0016.p (539 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid ligase II prote... 1070 0.0 ref|YP_740970.1| AMP-dependent synthetase and ligase [Alkalilimn... 606 e-171 ref|YP_003461755.1| acyl-CoA ligase (AMP-forming), exosortase sy... 605 e-171 ref|YP_003760939.1| acyl-CoA ligase [Nitrosococcus watsonii C-11... 604 e-170 ref|YP_413349.1| AMP-dependent synthetase and ligase [Nitrosospi... 598 e-168 ref|YP_747629.1| AMP-dependent synthetase and ligase [Nitrosomon... 597 e-168 ref|NP_841184.1| AMP-dependent synthetase and ligase [Nitrosomon... 597 e-168 ref|YP_343948.1| AMP-dependent synthetase and ligase [Nitrosococ... 596 e-168 ref|YP_003527651.1| acyl-CoA ligase (AMP-forming), exosortase sy... 589 e-166 ref|ZP_05046615.1| acyl-CoA ligase (AMP-forming), exosortase sys... 583 e-164 >ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] gb|ADJ64225.1| acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 539 Score = 1070 bits (2767), Expect = 0.0, Method: Compositional matrix adjust. Identities = 535/539 (99%), Positives = 538/539 (99%) Query: 1 VTPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY 60 +TPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY Sbjct: 1 MTPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY 60 Query: 61 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLS 120 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDC+VRVLV+SEPRHPALADRLS Sbjct: 61 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCAVRVLVSSEPRHPALADRLS 120 Query: 121 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA Sbjct: 121 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 Query: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL Sbjct: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 Query: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP 300 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP Sbjct: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP 300 Query: 301 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS Sbjct: 301 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 Query: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ Sbjct: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 Query: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSC 480 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAV PSSC Sbjct: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVVPSSC 480 Query: 481 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR 539 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR Sbjct: 481 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR 539 >ref|YP_740970.1| AMP-dependent synthetase and ligase [Alkalilimnicola ehrlichii MLHE-1] gb|ABI55480.1| AMP-dependent synthetase and ligase [Alkalilimnicola ehrlichii MLHE-1] Length = 533 Score = 606 bits (1563), Expect = e-171, Method: Compositional matrix adjust. Identities = 318/535 (59%), Positives = 379/535 (70%), Gaps = 12/535 (2%) Query: 3 PTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLE 62 P L+H+TLL+SA P + A+ YR DY LA + VAAAL GL ERVAVYL+ Sbjct: 4 PILLHETLLSSAGRAPSSPAVSYRDSSHDYDTLATACQSVAAALRECGLLRQERVAVYLD 63 Query: 63 KRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQC 122 KR ETV ALFGAA AG VFVP+NPLLK QV++IL+DC+VR+LVT+ R A+ L QC Sbjct: 64 KRPETVQALFGAAMAGGVFVPVNPLLKADQVAYILRDCNVRILVTTADRLKAIRQALVQC 123 Query: 123 PALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAIL 182 P L TV++V + P DW + A G R HR ID+D+AAIL Sbjct: 124 PDLHTVLVVGRSDTLGEEEPP------YRVHDWYDVVSTA---GPARPHRVIDSDMAAIL 174 Query: 183 YTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSG 242 YTSGSTGQPKGVVLSHRN+VAGAQSVA YL N P+D LLA LP SFDYGLSQL+TAFL+G Sbjct: 175 YTSGSTGQPKGVVLSHRNMVAGAQSVASYLDNRPEDHLLAALPFSFDYGLSQLTTAFLTG 234 Query: 243 ASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC--SLRYLTNSGGALP 300 A V+LLNYL PRD++ AV +GITGLA VPPLW QLA L WP +LRY+TNSGGA+P Sbjct: 235 AQVSLLNYLLPRDVIRAVEKQGITGLAAVPPLWIQLAQLEWPDAARKTLRYVTNSGGAMP 294 Query: 301 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 L LR LP + +LMYGLTEAFRSTYLPP E+DRRPDS+G+AIPNAEI VVR DGS Sbjct: 295 LKTLEALRAQLPATRFYLMYGLTEAFRSTYLPPEEVDRRPDSMGKAIPNAEIRVVREDGS 354 Query: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 PCAPGE GELVHRGALVA GYWNDP RTA+RFRPAPG+ L ELAVWSGD+VRMDE+ Sbjct: 355 PCAPGEPGELVHRGALVAMGYWNDPERTAQRFRPAPGQPGGLPQKELAVWSGDTVRMDEE 414 Query: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVV-AVAPSS 479 G+LYF+GR D+MIK SGYR+SP+EVEE ++ TGL + A +GV HP G +V V P S Sbjct: 415 GFLYFIGRRDEMIKTSGYRVSPNEVEEAVYGTGLVAEAAALGVPHPVLGHGIVLVVLPRS 474 Query: 480 CTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMK 534 + E + A R +P++MLPA + LPR+ NGK+DRK + D F + Sbjct: 475 TGVTAEELLNALRPRVPAFMLPAHVELRQQPLPRNANGKIDRKGLSTDFTDLFQE 529 >ref|YP_003461755.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Thioalkalivibrio sp. K90mix] gb|ADC73019.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Thioalkalivibrio sp. K90mix] Length = 532 Score = 605 bits (1559), Expect = e-171, Method: Compositional matrix adjust. Identities = 320/534 (59%), Positives = 384/534 (71%), Gaps = 13/534 (2%) Query: 4 TLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEK 63 TL+H+ +L SA+ P A+A+ +RG Y A+ +E A LLA GL +RVAVYL+K Sbjct: 5 TLLHEGILHSAERNPMASAITHRGVTQSYEAAASDIENAARRLLATGLQRQDRVAVYLDK 64 Query: 64 RMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCP 123 E V A+FGA+ AG V VP+NPLLK +QV+HIL+DC VRVLVTS RH AL D L+QCP Sbjct: 65 CPEAVHAMFGASRAGGVMVPVNPLLKASQVAHILRDCGVRVLVTSAVRHAALRDVLAQCP 124 Query: 124 ALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILY 183 L T++ VD A + P RL A+ AA R AHR ID D+AAILY Sbjct: 125 ELDTILFVDTPPDAETSGGP-----RL-----EAWQSAADFGLGRPAHRVIDTDMAAILY 174 Query: 184 TSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGA 243 TSGSTG+PKGVVLSHRNLVAGAQSVA YL N +D +L VLP SFDYG SQL+TAFL+GA Sbjct: 175 TSGSTGRPKGVVLSHRNLVAGAQSVAHYLHNRAEDCILCVLPFSFDYGFSQLTTAFLTGA 234 Query: 244 SVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC--SLRYLTNSGGALPR 301 TLLNYL PRD+L AV+ GITGLA VPPLW QLA + WP+ SLRY++NSGGA+P Sbjct: 235 HATLLNYLLPRDVLKAVSQGGITGLAAVPPLWIQLAEMEWPEAARRSLRYISNSGGAMPL 294 Query: 302 SVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSP 361 L +LR LP+ QV+LMYGLTEAFRSTYLPP E+DRRPDS+GRAIPNAE+MVVR DGS Sbjct: 295 KTLKRLRAHLPRTQVYLMYGLTEAFRSTYLPPEEVDRRPDSMGRAIPNAEVMVVREDGSR 354 Query: 362 CAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQG 421 CAPGE GELVHRGALVA GYWND ARTA+RFRPAPG+ L E+AVWSGD+VRMDE G Sbjct: 355 CAPGEPGELVHRGALVAMGYWNDAARTAQRFRPAPGQPEGLPGQEMAVWSGDTVRMDEDG 414 Query: 422 YLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVA-PSSC 480 YLYFVGR D+MIK SGYR+SP E+EEV +ATGL + A +G+AHPQ GQAVV VA P+ Sbjct: 415 YLYFVGRRDEMIKTSGYRVSPVEIEEVAYATGLVTEGAALGIAHPQLGQAVVLVALPAEP 474 Query: 481 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMK 534 + + + + R LP++M+P L LPR+ NGK+DRK + F + Sbjct: 475 SVTHDQLMALLRERLPAFMVPGHLELRSQSLPRNGNGKIDRKALAAEFEGLFQE 528 >ref|YP_003760939.1| acyl-CoA ligase [Nitrosococcus watsonii C-113] gb|ADJ28618.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Nitrosococcus watsonii C-113] Length = 534 Score = 604 bits (1558), Expect = e-170, Method: Compositional matrix adjust. Identities = 311/536 (58%), Positives = 381/536 (71%), Gaps = 11/536 (2%) Query: 2 TPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYL 61 T L H +L +A RP AL + Q L YA L + VE A LLALG+ P ER+AVYL Sbjct: 7 TNLLAHSLVLDNAFKRPDEPALIHGDQTLSYASLGSAVEACARGLLALGIAPSERIAVYL 66 Query: 62 EKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQ 121 KR ETV+ LFGAAAAG VFVPINPLLKP QV++IL+DC+VRVL+T++ R L D L++ Sbjct: 67 PKRPETVVTLFGAAAAGGVFVPINPLLKPRQVAYILRDCNVRVLITADNRTDLLQDELAE 126 Query: 122 CPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAI 181 C L+ +V+VDA A P + W + L G S HRRID+D+AAI Sbjct: 127 CHDLRNLVIVDAPTQAAKNLTHPT------AISWESLLSLGAGAPSL-GHRRIDSDMAAI 179 Query: 182 LYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLS 241 LYTSGSTG+PKGVVLSHRNLVAGAQSVA YL N+ DRLLAVLPLSFD G SQL+TAF + Sbjct: 180 LYTSGSTGRPKGVVLSHRNLVAGAQSVAQYLENNFNDRLLAVLPLSFDAGFSQLTTAFST 239 Query: 242 GASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC--SLRYLTNSGGAL 299 GASV L+ YL P+D++ ++A GITG+ VPPLW QLA L WP +LRY+ N+GG + Sbjct: 240 GASVVLMEYLLPKDVIKSIARHGITGITAVPPLWVQLASLAWPPEAVNTLRYIANTGGRM 299 Query: 300 PRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDG 359 P++ A LRR+LPQ +VFLMYGLTEAFRSTYLPP E+D+RPDSIG+AIPN EI V R DG Sbjct: 300 PKAATAALRRSLPQTKVFLMYGLTEAFRSTYLPPEEVDKRPDSIGKAIPNVEIQVARKDG 359 Query: 360 SPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDE 419 S C PGE GELVHRGALVA GYWNDP +TAERFRPAPG+ L E+AVWSGD+VRMDE Sbjct: 360 SLCLPGEPGELVHRGALVAMGYWNDPKKTAERFRPAPGQLPELPLTEIAVWSGDTVRMDE 419 Query: 420 QGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAP-S 478 G+LYF+GR D+MIK SGYR+SP EVEEVL+ TG + AVVG+ HP+ GQ +VA+ Sbjct: 420 DGFLYFIGRQDEMIKTSGYRVSPTEVEEVLYQTGFVAETAVVGIPHPKLGQGIVAIVKLD 479 Query: 479 SCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMK 534 S + E + ACRA LP++M+P + LPR+ NGK+DR+ + F + Sbjct: 480 SADFNPEDLLVACRAELPNFMVPLTIIA-SDALPRNANGKIDRRALAAKFESLFKE 534 >ref|YP_413349.1| AMP-dependent synthetase and ligase [Nitrosospira multiformis ATCC 25196] gb|ABB75957.1| AMP-dependent synthetase and ligase [Nitrosospira multiformis ATCC 25196] Length = 531 Score = 598 bits (1541), Expect = e-168, Method: Compositional matrix adjust. Identities = 320/533 (60%), Positives = 382/533 (71%), Gaps = 9/533 (1%) Query: 5 LIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKR 64 LI D + SA AL Y+ +L YA LA + V LLALGLG GERVAVYLEKR Sbjct: 4 LIQDLVFESANRFSDKEALVYQRNRLSYADLAREIGLVREGLLALGLGRGERVAVYLEKR 63 Query: 65 METVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPA 124 +E V +LFGAAAAG FVP+NPLLKP QV++IL+DC+VR+LVTS R L + L QC Sbjct: 64 IEAVTSLFGAAAAGGAFVPVNPLLKPEQVAYILRDCNVRILVTSVERLKLLVEALPQCHD 123 Query: 125 LQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYT 184 L V++ D A AA P L + WN + A G + DAD+AAILYT Sbjct: 124 LHAVIVTDRAEAAMPVPG-------LTVVSWNDAVTAGKPDGRAVSRPATDADMAAILYT 176 Query: 185 SGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGAS 244 SGSTG+PKGVVLSHRNLVAGA SV+ YL N+P DR+L+VLPLSFDYGLSQL+TAF GA+ Sbjct: 177 SGSTGKPKGVVLSHRNLVAGAASVSQYLKNNPDDRILSVLPLSFDYGLSQLTTAFRVGAT 236 Query: 245 VTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALPRSVL 304 L+NYL PRDI+ + E ITGLA VPPLW QLA L WP+ LRY+TNSGGA+PR+ L Sbjct: 237 GVLMNYLLPRDIIDGLKEEKITGLAAVPPLWIQLAQLSWPKDIPLRYITNSGGAMPRATL 296 Query: 305 AQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAP 364 LR LP A VFLMYGLTEAFRST+LPP E+D RPDSIG+AIPNAE++VVR DGSPCAP Sbjct: 297 DLLRNVLPNAAVFLMYGLTEAFRSTFLPPEEVDTRPDSIGKAIPNAEVLVVREDGSPCAP 356 Query: 365 GEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLY 424 GE GELVHRG LV+ GYWND +T ERFRP P RE L PELAVWSGD+VRMDE G+LY Sbjct: 357 GEPGELVHRGPLVSMGYWNDKEKTTERFRPVPCREPGLTIPELAVWSGDTVRMDEAGFLY 416 Query: 425 FVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAP--SSCTA 482 F+GR D+MIK SGYR+SP EVEEV++ T L + A VGV HP G A+V +A + A Sbjct: 417 FIGRRDEMIKTSGYRVSPTEVEEVVYGTELVGEVAAVGVPHPVLGHAIVLIATPRAGLKA 476 Query: 483 SLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKA 535 + EA+ AC+ HLP++MLP+ + G LPR+PNGK+DRK + Q L D FM+A Sbjct: 477 ADEALLAACKTHLPAFMLPSRIEYREGSLPRNPNGKIDRKRLAQELHDIFMEA 529 >ref|YP_747629.1| AMP-dependent synthetase and ligase [Nitrosomonas eutropha C91] gb|ABI59664.1| AMP-dependent synthetase and ligase [Nitrosomonas eutropha C91] Length = 529 Score = 597 bits (1540), Expect = e-168, Method: Compositional matrix adjust. Identities = 307/535 (57%), Positives = 370/535 (69%), Gaps = 19/535 (3%) Query: 4 TLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEK 63 +L H+ + SA + AL R + L Y LA V+ A L LGL GERVAVYLEK Sbjct: 3 SLFHELVYQSADRNIDSVALIDRKRHLSYPALAEAVQSFAGTLHTLGLSRGERVAVYLEK 62 Query: 64 RMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCP 123 R+ETV+ALFG +AAG FVP+NPLLK QV++IL DC+VRVLVTS R L LSQC Sbjct: 63 RLETVIALFGTSAAGGAFVPVNPLLKAEQVAYILNDCNVRVLVTSVDRFSLLNTALSQCH 122 Query: 124 ALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAH--RRIDADIAAI 181 L TV+ VD A + + P L W A S H R ID+D+AAI Sbjct: 123 DLHTVITVDEAQESSSIPG-------LNVTSWGQ----AQISSSEAVHLPRCIDSDMAAI 171 Query: 182 LYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLS 241 LYTSGSTG+PKGVVLSHRNLV GA+SV+ YL N DR+LAVLPLSFDYGLSQL+TAF Sbjct: 172 LYTSGSTGKPKGVVLSHRNLVIGAKSVSQYLNNQSNDRILAVLPLSFDYGLSQLNTAFYV 231 Query: 242 GASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALPR 301 GA+ L+NYL PRDIL + E ITGLA VPPLW QLA L W +LRY+TNSGGA+PR Sbjct: 232 GATAILMNYLLPRDILSIIKREQITGLAAVPPLWTQLAQLDWQGVQTLRYITNSGGAMPR 291 Query: 302 SVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSP 361 + LA+LR ALP ++FLMYGLTEAFRSTYLPP E D+RPDS+G+AIPNAE+MV+R DGS Sbjct: 292 TTLARLREALPSTEIFLMYGLTEAFRSTYLPPEEADKRPDSMGKAIPNAEVMVLREDGSH 351 Query: 362 CAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQG 421 CAPGE GELVHRG LV+ GYWNDP +T+ RFRP R++ L PELAVWSGD+VRMDE+G Sbjct: 352 CAPGEPGELVHRGPLVSLGYWNDPDKTSARFRPLAPRQSGLTIPELAVWSGDTVRMDEEG 411 Query: 422 YLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSCT 481 YLYF+GR DDMIK SGYR+SP E+EEV++AT + A G HP GQA+V +A Sbjct: 412 YLYFIGRRDDMIKTSGYRVSPTEIEEVIYATETVAETAAFGAPHPTLGQAIVVIAVPKTG 471 Query: 482 ASL--EAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMK 534 L + + AC+ HLP++M P + LPR+PNGK+DRK +LA F + Sbjct: 472 IELKQDTLMAACKNHLPAFMQPGLIELRQTSLPRNPNGKIDRK----SLASEFQQ 522 >ref|NP_841184.1| AMP-dependent synthetase and ligase [Nitrosomonas europaea ATCC 19718] emb|CAD85036.1| AMP-dependent synthetase and ligase [Nitrosomonas europaea ATCC 19718] Length = 527 Score = 597 bits (1538), Expect = e-168, Method: Compositional matrix adjust. Identities = 310/537 (57%), Positives = 379/537 (70%), Gaps = 17/537 (3%) Query: 4 TLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEK 63 L H+ + SA P +TAL + + L YA L+ V+ +A+AL LGLG GER AVYLEK Sbjct: 3 NLFHELIYQSAARYPDSTALIDQKRHLSYAALSEAVQSIASALHTLGLGRGERSAVYLEK 62 Query: 64 RMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCP 123 R+ETV+ALFGA+AAG FVP+NPLLK QV++IL+DC+VR+L+TS R L+ L QC Sbjct: 63 RLETVIALFGASAAGGAFVPVNPLLKAEQVAYILKDCNVRILITSAERLDLLSPVLPQCH 122 Query: 124 ALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILY 183 L TV++ A L L + W Q + R ID+D+AAILY Sbjct: 123 DLHTVIITGDLHKAS--------LPGLNVVSWR---QTQTLSDAARLPDCIDSDMAAILY 171 Query: 184 TSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGA 243 TSGSTG+PKGVVLSHRNLV GA SV+ YL N P DR+LAVLPLSFDYGL+QL+TAF +GA Sbjct: 172 TSGSTGKPKGVVLSHRNLVTGAISVSRYLNNRPDDRILAVLPLSFDYGLNQLNTAFYTGA 231 Query: 244 SVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALPRSV 303 + L+NYL PRDIL V E +TGLA VPPLW QLA L W SLRY+TNSGGA+PR+ Sbjct: 232 TAILMNYLLPRDILTTVKQEQVTGLAAVPPLWAQLAQLDWKDAQSLRYITNSGGAMPRAT 291 Query: 304 LAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCA 363 LA LR ALP Q+FLMYGLTEAFRSTYLPP E+D+RPDS+G+AIPNAE+MV+R DGS CA Sbjct: 292 LAHLRSALPDTQIFLMYGLTEAFRSTYLPPGEVDKRPDSMGKAIPNAEVMVLREDGSHCA 351 Query: 364 PGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYL 423 PGE GELVHRG LV+ GYWND +TA FRP R++ L PELAVWSGD+VRMD++GYL Sbjct: 352 PGEPGELVHRGPLVSLGYWNDADKTAACFRPLTPRQSGLTIPELAVWSGDTVRMDDEGYL 411 Query: 424 YFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSCTAS 483 YF+GR DDMIK SGYR+SP EVEEV++AT + A GV HP GQA+V VA + Sbjct: 412 YFIGRRDDMIKTSGYRVSPTEVEEVIYATEKVAEAAAFGVPHPTLGQAIVVVAVPRTGFA 471 Query: 484 L--EAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHA 538 L + +Q AC+ HLP++M PA + LPR+PNGK+DRK+ LA F +A A Sbjct: 472 LDRDVLQSACKQHLPAFMQPALIELRQTSLPRNPNGKIDRKM----LAGEFQQAFQA 524 >ref|YP_343948.1| AMP-dependent synthetase and ligase [Nitrosococcus oceani ATCC 19707] gb|ABA58418.1| AMP-dependent synthetase and ligase [Nitrosococcus oceani ATCC 19707] Length = 538 Score = 596 bits (1536), Expect = e-168, Method: Compositional matrix adjust. Identities = 310/524 (59%), Positives = 375/524 (71%), Gaps = 15/524 (2%) Query: 4 TLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEK 63 +L+H +L +A P A+AL + Q L YA L VE A LLALGL ERVA+YL K Sbjct: 9 SLVHSLVLDNALKGPDASALVHGDQTLTYASLGETVEACARGLLALGLASSERVAIYLPK 68 Query: 64 RMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCP 123 R ETV+ LFGAAAAG VFVPINPLLKP QV+HIL+DC+VRVLVT+ R L D L++C Sbjct: 69 RPETVVTLFGAAAAGGVFVPINPLLKPRQVAHILRDCNVRVLVTASNRIDFLQDALAECH 128 Query: 124 ALQTVVLVDAAAAAPATPAPPAPLG--RLATLDWNAFLQAAPGPGSRRAHRRIDADIAAI 181 L+++V+VDA A P + RL +L Q +PG HRRID+D+AAI Sbjct: 129 DLRSLVIVDAPTQTIEKLAQPMAISWERLLSL---GTTQQSPG------HRRIDSDMAAI 179 Query: 182 LYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLS 241 LYTSGSTG+PKGVVLSHRNLVAGAQSVA YL N+ DRLLAVLPLSFD G SQL+TAF Sbjct: 180 LYTSGSTGRPKGVVLSHRNLVAGAQSVAQYLENNSNDRLLAVLPLSFDAGFSQLTTAFSV 239 Query: 242 GASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWP--QGCSLRYLTNSGGAL 299 GASV L+ YL P+D++ ++ GITG+ VPPLW QLA L WP +LRY+ N+GG + Sbjct: 240 GASVVLMEYLLPKDVIKSITRHGITGITAVPPLWVQLASLAWPPEAADTLRYIANTGGRM 299 Query: 300 PRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDG 359 P++ LRR+LPQ +VFLMYGLTEAFRSTYLPP E+D+RPDSIG+AIPN EI V R DG Sbjct: 300 PKAATTALRRSLPQTKVFLMYGLTEAFRSTYLPPEEVDKRPDSIGKAIPNVEIQVAREDG 359 Query: 360 SPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDE 419 S C PGE GELVHRG LVA GYWNDP +TAERFRP PG+ L E+AVWSGD+VRMDE Sbjct: 360 SLCLPGESGELVHRGVLVAMGYWNDPKKTAERFRPTPGQPPELPLTEIAVWSGDTVRMDE 419 Query: 420 QGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVA-VAPS 478 G+ YF+GR D+MIK SGYR+SP EVEEVL+ GL + AVVGV HP+ GQ +VA V P+ Sbjct: 420 DGFFYFIGRQDEMIKTSGYRVSPTEVEEVLYQAGLVAEAAVVGVLHPKLGQGIVAIVKPN 479 Query: 479 SCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRK 522 E + CRA LP++M+P A+ LPR+ NGK+DR+ Sbjct: 480 KDNFDPEDLLATCRAELPNFMVPLAVI-VSENLPRNTNGKIDRR 522 >ref|YP_003527651.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Nitrosococcus halophilus Nc4] gb|ADE15264.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Nitrosococcus halophilus Nc4] Length = 537 Score = 589 bits (1518), Expect = e-166, Method: Compositional matrix adjust. Identities = 313/537 (58%), Positives = 383/537 (71%), Gaps = 20/537 (3%) Query: 5 LIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKR 64 L+H +L +A RP ATA+ + + L+YA L +E LALGL P ERVAVYL K Sbjct: 10 LVHHFVLDNAAKRPDATAVVHGRRILNYAHLGEAIEACGRGFLALGLAPSERVAVYLPKC 69 Query: 65 METVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPA 124 ETV ALFGAAAAG VFVPINPLLKP QV++IL+DC+VRVLVT+ R L + L++C Sbjct: 70 PETVTALFGAAAAGGVFVPINPLLKPRQVAYILRDCNVRVLVTAGDRIDLLQNELAECHD 129 Query: 125 LQTVVLVDAAAAAPATPAPPA----PLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 L+++VL+D PPA L L W + G HRRID+D+AA Sbjct: 130 LRSLVLID----------PPAQGVEELAHLTIASWANLISL--GANQPLGHRRIDSDMAA 177 Query: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 ILYTSGSTG+PKGVVLSHRNL+AGAQSVA YLGN+P DRLLA+LPLSFD G SQL+TAF Sbjct: 178 ILYTSGSTGRPKGVVLSHRNLIAGAQSVAHYLGNNPNDRLLAILPLSFDAGFSQLTTAFS 237 Query: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC--SLRYLTNSGGA 298 +GASV L+ YL P+D++ V E ITG+ VPPLW QLA L WP SLRY+ N+GG Sbjct: 238 AGASVALMEYLLPKDVIKNVVRERITGITAVPPLWIQLAPLTWPPEAVDSLRYIANTGGR 297 Query: 299 LPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPD 358 +P++V A LRR+LPQ +VFLMYGLTEAFRSTYLPP E+D+RPDSIG+AIPNAEI+VVR D Sbjct: 298 MPKAVTATLRRSLPQTKVFLMYGLTEAFRSTYLPPEEVDKRPDSIGKAIPNAEILVVRED 357 Query: 359 GSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMD 418 GS CAPGE GELVHRG LVA GYWN P +T+ERFRPAPG+ A L E+AVWSGD+VRMD Sbjct: 358 GSLCAPGEPGELVHRGPLVAMGYWNAPEKTSERFRPAPGQPAELPLTEIAVWSGDTVRMD 417 Query: 419 EQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVA-P 477 E+G+LYFV R D+MIK SGYR+SP EVEEVL+ TGL + AVVG HP+ GQ ++AV P Sbjct: 418 EEGFLYFVTRQDEMIKTSGYRVSPTEVEEVLYETGLISEVAVVGAPHPKLGQGIIAVVKP 477 Query: 478 SSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMK 534 + E + ACRA LP++M+P + LPR+ NGK+DR+ + F + Sbjct: 478 NQADFDPEHLLSACRAELPNFMVPLTVIT-SNALPRNTNGKIDRRALAAEFESLFKE 533 >ref|ZP_05046615.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Nitrosococcus oceani AFC27] gb|EDZ66711.1| acyl-CoA ligase (AMP-forming), exosortase system type 1 associated [Nitrosococcus oceani AFC27] Length = 510 Score = 583 bits (1503), Expect = e-164, Method: Compositional matrix adjust. Identities = 302/500 (60%), Positives = 361/500 (72%), Gaps = 15/500 (3%) Query: 28 QQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLALFGAAAAGLVFVPINPL 87 Q L YA L VE A LLALGL ERVA+YL KR ETV+ LFGAAAAG VFVPINPL Sbjct: 5 QTLTYASLGETVEACARGLLALGLASSERVAIYLPKRPETVVTLFGAAAAGGVFVPINPL 64 Query: 88 LKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVVLVDAAAAAPATPAPPAPL 147 LKP QV+HIL+DC+VRVLVT+ R L D L++C L+++V+VDA A P + Sbjct: 65 LKPRQVAHILRDCNVRVLVTASNRIDFLQDALAECHDLRSLVIVDAPTQTIEKLAQPMAI 124 Query: 148 G--RLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGVVLSHRNLVAGA 205 RL +L Q +PG HRRID+D+AAILYTSGSTG+PKGVVLSHRNLVAGA Sbjct: 125 SWERLLSL---GTTQQSPG------HRRIDSDMAAILYTSGSTGRPKGVVLSHRNLVAGA 175 Query: 206 QSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTLLNYLAPRDILHAVASEGI 265 QSVA YL N+ DRLLAVLPLSFD G SQL+TAF GASV L+ YL P+D++ ++ GI Sbjct: 176 QSVAQYLENNSNDRLLAVLPLSFDAGFSQLTTAFSVGASVVLMEYLLPKDVIKSITRHGI 235 Query: 266 TGLAGVPPLWQQLAGLPWP--QGCSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLT 323 TG+ VPPLW QLA L WP +LRY+ N+GG +P++ LRR+LPQ +VFLMYGLT Sbjct: 236 TGITAVPPLWVQLASLAWPPEAADTLRYIANTGGRMPKAATTALRRSLPQTKVFLMYGLT 295 Query: 324 EAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWN 383 EAFRSTYLPP E+D+RPDSIG+AIPN EI V R DGS C PGE GELVHRG LVA GYWN Sbjct: 296 EAFRSTYLPPEEVDKRPDSIGKAIPNVEIQVAREDGSLCLPGESGELVHRGVLVAMGYWN 355 Query: 384 DPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPH 443 DP +TAERFRP PG+ L E+AVWSGD+VRMDE G+ YF+GR D+MIK SGYR+SP Sbjct: 356 DPKKTAERFRPTPGQPPELPLTEIAVWSGDTVRMDEDGFFYFIGRQDEMIKTSGYRVSPT 415 Query: 444 EVEEVLHATGLADQFAVVGVAHPQQGQAVVA-VAPSSCTASLEAVQQACRAHLPSYMLPA 502 EVEEVL+ GL + AVVGV HP+ GQ +VA V P+ E + CRA LP++M+P Sbjct: 416 EVEEVLYQAGLVAEAAVVGVLHPKLGQGIVAIVKPNKDNFDPEDLLATCRAELPNFMVPL 475 Query: 503 ALHRWPGELPRSPNGKLDRK 522 A+ LPR+ NGK+DR+ Sbjct: 476 AVI-VSENLPRNTNGKIDRR 494