BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0048.p (259 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Her... 527 e-148 ref|YP_004619617.1| unsaturated acyl-CoA hydratase [Ramlibacter ... 335 3e-90 ref|YP_001790030.1| enoyl-CoA hydratase [Leptothrix cholodnii SP... 328 5e-88 gb|ABW35795.1| putative enoyl-CoA hydratase/isomerase [unculture... 324 6e-87 ref|ZP_08406557.1| enoyl-CoA hydratase [Hylemonella gracilis ATC... 324 9e-87 ref|YP_001242882.1| enoyl-CoA hydratase [Bradyrhizobium sp. BTAi... 324 1e-86 ref|YP_001203115.1| enoyl-CoA hydratase [Bradyrhizobium sp. ORS ... 323 2e-86 ref|YP_549923.1| enoyl-CoA hydratase [Polaromonas sp. JS666] >gi... 321 8e-86 emb|CBA32364.1| hypothetical protein Csp_D31770 [Curvibacter put... 320 1e-85 ref|YP_980845.1| enoyl-CoA hydratase [Polaromonas naphthalenivor... 316 2e-84 >ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] gb|ADJ65477.1| enoyl-CoA hydratase/carnithine racemase protein [Herbaspirillum seropedicae SmR1] Length = 259 Score = 527 bits (1358), Expect = e-148, Method: Compositional matrix adjust. Identities = 259/259 (100%), Positives = 259/259 (100%) Query: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH Sbjct: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 Query: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI Sbjct: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 Query: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY Sbjct: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 Query: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD Sbjct: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 Query: 241 TKGRLAAFLDLKQNKVKPV 259 TKGRLAAFLDLKQNKVKPV Sbjct: 241 TKGRLAAFLDLKQNKVKPV 259 >ref|YP_004619617.1| unsaturated acyl-CoA hydratase [Ramlibacter tataouinensis TTB310] gb|AEG93598.1| Unsaturated acyl-CoA hydratase [Ramlibacter tataouinensis TTB310] Length = 278 Score = 335 bits (860), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 163/250 (65%), Positives = 194/250 (77%) Query: 8 LDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGLDL 67 LDI GA+A I LNR AKRNAL+D LV A+RD FENLP V+ A+L G GEHFCAGLDL Sbjct: 24 LDIDIRGAVAVIRLNRPAKRNALSDALVLAVRDAFENLPATVRAAVLGGSGEHFCAGLDL 83 Query: 68 SELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEPST 127 SEL+ERD LHHSRMWHAA E++ +G VPV+A LHGAV+GGGLELAAA HIRVA+ + Sbjct: 84 SELKERDAGAGLHHSRMWHAALERVQYGPVPVVAALHGAVVGGGLELAAACHIRVADDTA 143 Query: 128 FYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAGAG 187 FY LPEG RG+FVGGGG+VRI RLIG ARMTDMMLTGRV +AEEG +AG QY V AG Sbjct: 144 FYALPEGSRGIFVGGGGAVRIPRLIGAARMTDMMLTGRVYNAEEGERAGFAQYRVPAGQA 203 Query: 188 MDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAA 247 DKA LA+R+A NAP+TNY ++H LPRI +Q G +TE+LMAA+A S P+ K R+ A Sbjct: 204 FDKALALAQRVAENAPLTNYALVHALPRIAEQPADQGFLTEALMAAIAQSAPEAKARVRA 263 Query: 248 FLDLKQNKVK 257 FL+ + +KVK Sbjct: 264 FLEGRADKVK 273 >ref|YP_001790030.1| enoyl-CoA hydratase [Leptothrix cholodnii SP-6] gb|ACB33265.1| Enoyl-CoA hydratase/isomerase [Leptothrix cholodnii SP-6] Length = 273 Score = 328 bits (841), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 159/251 (63%), Positives = 193/251 (76%) Query: 6 ELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGL 65 +LL I +G + I +NR AKRNAL+D L++ + F NLP+GV A++ G GEHFCAGL Sbjct: 20 DLLQISCNGKVLHICINRPAKRNALSDTLIQQLHTAFVNLPDGVAAAVISGAGEHFCAGL 79 Query: 66 DLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEP 125 DLSEL ERD + HSRMWHAAF+ I FG+VPV+A LHGAV+GGGLELA+A H+RVA+ Sbjct: 80 DLSELVERDIGSGVKHSRMWHAAFDLIQFGQVPVVAALHGAVVGGGLELASACHVRVADE 139 Query: 126 STFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAG 185 ST++ LPEGQRG+FVGGGGSVRI RL+GVARMTD+MLTGRV A EG AG+ QY V G Sbjct: 140 STYFALPEGQRGIFVGGGGSVRIPRLMGVARMTDLMLTGRVYDAAEGVAAGLAQYLVPQG 199 Query: 186 AGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRL 245 +D+A LA+RIA NAP++NY VMH LPRI DQS+ DGL TESLMAAVA S PD + RL Sbjct: 200 TSVDRAIALAQRIATNAPLSNYAVMHALPRIADQSMSDGLFTESLMAAVAESTPDAQDRL 259 Query: 246 AAFLDLKQNKV 256 AFL+ + KV Sbjct: 260 RAFLEKRAGKV 270 >gb|ABW35795.1| putative enoyl-CoA hydratase/isomerase [uncultured bacterium] Length = 294 Score = 324 bits (831), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 157/250 (62%), Positives = 191/250 (76%) Query: 8 LDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGLDL 67 + ++ GA+A +TL R KRNAL+D L+ AIRD F+NLP + A++ G GEHFCAGLDL Sbjct: 42 VTLEMRGAVAVVTLRRPGKRNALSDALIEAIRDTFQNLPAEARAAVIDGEGEHFCAGLDL 101 Query: 68 SELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEPST 127 SEL+ERD E +HHSR WH A + + FGRVPVIA LHGAV+GGGLELA+A+HIRVA+ ST Sbjct: 102 SELKERDAGEGVHHSRGWHVALDAVQFGRVPVIAALHGAVVGGGLELASASHIRVADDST 161 Query: 128 FYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAGAG 187 FY LPEG RG+FVGGGGSVRI +LIGVARMTDMMLTGRV +A EG + G QY V G Sbjct: 162 FYALPEGTRGIFVGGGGSVRIPKLIGVARMTDMMLTGRVYNAVEGERLGFAQYLVPQGTA 221 Query: 188 MDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAA 247 +DKA ELA RIA NAP+TNY +MH LPRI +Q G +TE+LMA++A S P+ K R+ A Sbjct: 222 LDKAVELATRIATNAPLTNYALMHALPRIAEQPADHGFLTEALMASIAQSAPEAKARVRA 281 Query: 248 FLDLKQNKVK 257 FL+ K KVK Sbjct: 282 FLEGKAAKVK 291 >ref|ZP_08406557.1| enoyl-CoA hydratase [Hylemonella gracilis ATCC 19624] gb|EGI76300.1| enoyl-CoA hydratase [Hylemonella gracilis ATCC 19624] Length = 263 Score = 324 bits (830), Expect = 9e-87, Method: Compositional matrix adjust. Identities = 159/248 (64%), Positives = 190/248 (76%), Gaps = 3/248 (1%) Query: 13 HGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEG---VKVAILRGHGEHFCAGLDLSE 69 H IA I L+R KRNALND L+ A+RD FENLP G V+ A++ G GEHFCAGLDLSE Sbjct: 14 HDEIAVIRLSRPVKRNALNDALILALRDLFENLPTGEGGVRAAVIAGEGEHFCAGLDLSE 73 Query: 70 LRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEPSTFY 129 L+ERD E LHHSRMWHAA E++ +G VPVIA LHGAV+GGGLELA+A HIRVA+ +TFY Sbjct: 74 LKERDAGEGLHHSRMWHAALERVQYGPVPVIAALHGAVVGGGLELASACHIRVADDTTFY 133 Query: 130 GLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAGAGMD 189 LPEG RG+FVGGGG+VRI RLIG ARMTDMMLTGRV +A +G +AG QY V G D Sbjct: 134 ALPEGSRGIFVGGGGAVRIPRLIGAARMTDMMLTGRVYNAADGEKAGFAQYLVPQGTAFD 193 Query: 190 KARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAAFL 249 KA ELA R+A+NAP+TNY ++H LPRI +Q G +TE++MAA+A S P+ K R+ AFL Sbjct: 194 KALELAARVAQNAPLTNYALIHALPRIAEQPADQGFLTEAMMAAIAQSAPEAKARVRAFL 253 Query: 250 DLKQNKVK 257 D K KVK Sbjct: 254 DGKAAKVK 261 >ref|YP_001242882.1| enoyl-CoA hydratase [Bradyrhizobium sp. BTAi1] gb|ABQ38976.1| trans-feruloyl-CoA hydratase / vanillin synthase [Bradyrhizobium sp. BTAi1] Length = 267 Score = 324 bits (830), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 162/254 (63%), Positives = 191/254 (75%) Query: 6 ELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGL 65 LL I+Q GA+ I LNR AKRNALND ++ AI DCF LP+ V+V ++ G G++F +GL Sbjct: 14 SLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGDCFAGLPDDVRVVVIHGLGDNFSSGL 73 Query: 66 DLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEP 125 DLSELRERD TE L HS+MWH F++I + RVPVIA L GAVIGGGLELA AAHIRVAEP Sbjct: 74 DLSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEP 133 Query: 126 STFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAG 185 S ++ LPEGQRG+FVGGGGSVR+ RLIGVARM DMMLTGRV SA EG G QY V+AG Sbjct: 134 SAYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGSSYGFAQYLVEAG 193 Query: 186 AGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRL 245 A M KA ELA +IA NAP+TN+ V+ LP I + + Q GL+ ESLMA VA SD + K R+ Sbjct: 194 ASMTKAMELAGKIASNAPLTNFAVLQALPMIAEANPQTGLLMESLMATVAQSDQEAKRRI 253 Query: 246 AAFLDLKQNKVKPV 259 AFLD K KVKP Sbjct: 254 RAFLDHKTAKVKPT 267 >ref|YP_001203115.1| enoyl-CoA hydratase [Bradyrhizobium sp. ORS 278] emb|CAL74878.1| putative 3-hydroxybutyryl-CoA dehydratase [Bradyrhizobium sp. ORS 278] Length = 266 Score = 323 bits (827), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 159/254 (62%), Positives = 191/254 (75%) Query: 6 ELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGL 65 LL I+Q GA+ I LNR AKRNALND ++ AI +CF +LP+ ++ ++ G G++F +GL Sbjct: 13 SLLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVVIHGLGDNFSSGL 72 Query: 66 DLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEP 125 DLSELRERD TE L HS+MWH F++I + RVPVIA L GAVIGGGLELA AAHIRVAEP Sbjct: 73 DLSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEP 132 Query: 126 STFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAG 185 S ++ LPEGQRG+FVGGGGSVR+ RLIGVARM DMMLTGRV SA EG G QY V+AG Sbjct: 133 SAYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGSSYGFAQYLVEAG 192 Query: 186 AGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRL 245 A KA ELA +IA NAP+TN+ V+ LP I + + Q GL+ ESLMA VA SD + KGR+ Sbjct: 193 AATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMATVAQSDKEAKGRI 252 Query: 246 AAFLDLKQNKVKPV 259 AFLD K KVKP Sbjct: 253 RAFLDHKTAKVKPT 266 >ref|YP_549923.1| enoyl-CoA hydratase [Polaromonas sp. JS666] gb|ABE45025.1| vanillin synthase / trans-feruloyl-CoA hydratase [Polaromonas sp. JS666] Length = 264 Score = 321 bits (822), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 157/262 (59%), Positives = 194/262 (74%), Gaps = 5/262 (1%) Query: 1 MNQHSELLDIQ-----QHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILR 55 M ++ DIQ H IA I L+R AKRNA+ND L+ A+RD FE LP V+ A++ Sbjct: 1 MTSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIH 60 Query: 56 GHGEHFCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELA 115 G GEHFCAGLDL EL ERD + +HHSRMWHAA E++ FG VPVIA L GAV+GGGLELA Sbjct: 61 GEGEHFCAGLDLGELEERDAGQGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELA 120 Query: 116 AAAHIRVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQA 175 +A HIRVA+ + FY LPEG RG+FVGGGGSVRI +LIGVARMTDMMLTGRV +A++G +A Sbjct: 121 SACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVARMTDMMLTGRVYNAQDGERA 180 Query: 176 GIPQYSVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVA 235 G QY V G DKA ELA R+A+NAP+TNY +MH LPRI +Q + G +TE+LMAA+A Sbjct: 181 GFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIA 240 Query: 236 GSDPDTKGRLAAFLDLKQNKVK 257 S P+ K R+ AFL+ + KV+ Sbjct: 241 QSAPEAKDRVRAFLEGRAAKVQ 262 >emb|CBA32364.1| hypothetical protein Csp_D31770 [Curvibacter putative symbiont of Hydra magnipapillata] Length = 276 Score = 320 bits (820), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 157/254 (61%), Positives = 193/254 (75%), Gaps = 2/254 (0%) Query: 4 HSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCA 63 H E L +Q +A I L R AKRNALND L+ A+RD FEN+PEGV+ A++ G G HFCA Sbjct: 22 HFERLGEEQE--VAVIRLTRGAKRNALNDGLILALRDLFENMPEGVRAAVIAGEGPHFCA 79 Query: 64 GLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVA 123 GLDLSEL+ERD + LHHSRMWHAA E++ +G VPVIA L GAV+GGGLELA+A HIRVA Sbjct: 80 GLDLSELKERDAGQGLHHSRMWHAALERVQYGPVPVIAALQGAVVGGGLELASACHIRVA 139 Query: 124 EPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVD 183 + STFY LPEG RG+FVGGGG+VRI +LIG ARMTDMMLTGRV +A++G + G+ QY V Sbjct: 140 DESTFYALPEGSRGIFVGGGGAVRIPKLIGAARMTDMMLTGRVYNAQDGERIGLAQYLVP 199 Query: 184 AGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKG 243 GA DKA ELA R+A+NAP+TNY +MH LPRI +Q G TE++MAA+A S P+ K Sbjct: 200 EGAAFDKAVELAVRVAQNAPLTNYALMHALPRIAEQPADQGFFTEAMMAAIAQSAPEAKT 259 Query: 244 RLAAFLDLKQNKVK 257 RL FL+ + KV+ Sbjct: 260 RLNDFLEGRAAKVR 273 >ref|YP_980845.1| enoyl-CoA hydratase [Polaromonas naphthalenivorans CJ2] gb|ABM35924.1| vanillin synthase / trans-feruloyl-CoA hydratase [Polaromonas naphthalenivorans CJ2] Length = 260 Score = 316 bits (810), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 153/242 (63%), Positives = 187/242 (77%) Query: 16 IAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHFCAGLDLSELRERDT 75 +A + L R AKRNAL+D LV A+R+ FE LP V+ A++ G G HFCAGLDLSEL+ERD Sbjct: 17 VAVVRLTRGAKRNALSDGLVLALRNIFEQLPATVRAAVIDGEGPHFCAGLDLSELKERDA 76 Query: 76 TESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRVAEPSTFYGLPEGQ 135 LHHSRMWHAA E++ +G VPV+A LHGAV+GGGLELA+A HIRVA+ +TFY LPEG Sbjct: 77 GAGLHHSRMWHAALERVQYGPVPVVAALHGAVVGGGLELASACHIRVADETTFYALPEGS 136 Query: 136 RGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSVDAGAGMDKARELA 195 RG+FVGGGG+VRI RLIG ARMTDMMLTGRV +A++G + G+ QY V AG DKA ELA Sbjct: 137 RGIFVGGGGAVRIPRLIGAARMTDMMLTGRVYNAQDGERIGLAQYLVPAGTAFDKALELA 196 Query: 196 ERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAAFLDLKQNK 255 +RIA+NAP+TNY ++H LPRI +Q G MTE+LMAA+A S P+ K R+ AFLD K K Sbjct: 197 KRIAQNAPLTNYALVHALPRIAEQPADQGYMTEALMAAIAQSAPEAKQRVRAFLDGKAEK 256 Query: 256 VK 257 V Sbjct: 257 VS 258