BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0031.p (420 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carbox... 632 e-179 ref|YP_004752729.1| hydrolase or acyltransferase-like protein [C... 626 e-177 ref|YP_001022719.1| hydrolase or acyltransferase-like protein [M... 514 e-143 gb|AAX47260.1| hydrolase/acyltransferase [Delftia tsuruhatensis] 258 2e-66 gb|ACO92642.1| putative hydrolase/acyltransferase [Burkholderia ... 217 2e-54 emb|CBE67933.1| Alpha/beta hydrolase fold [NC10 bacterium 'Dutch... 217 3e-54 ref|YP_548688.1| 3-oxoadipate enol-lactonase [Polaromonas sp. JS... 214 2e-53 ref|ZP_06685390.1| 4-carboxymuconolactone decarboxylase [Achromo... 206 9e-51 ref|YP_001563021.1| alpha/beta hydrolase fold protein [Delftia a... 202 1e-49 gb|EFV87460.1| carboxymuconolactone decarboxylase [Achromobacter... 200 4e-49 >ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] gb|ADJ65460.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 632 bits (1631), Expect = e-179, Method: Compositional matrix adjust. Identities = 316/316 (100%), Positives = 316/316 (100%) Query: 105 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL 164 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL Sbjct: 1 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL 60 Query: 165 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL 224 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL Sbjct: 61 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL 120 Query: 225 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA 284 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA Sbjct: 121 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA 180 Query: 285 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC 344 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC Sbjct: 181 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC 240 Query: 345 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE 404 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE Sbjct: 241 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE 300 Query: 405 QPALFAELVCGFIDGI 420 QPALFAELVCGFIDGI Sbjct: 301 QPALFAELVCGFIDGI 316 >ref|YP_004752729.1| hydrolase or acyltransferase-like protein [Collimonas fungivorans Ter331] gb|AEK61906.1| hydrolase or acyltransferase-like protein [Collimonas fungivorans Ter331] Length = 420 Score = 626 bits (1615), Expect = e-177, Method: Compositional matrix adjust. Identities = 321/417 (76%), Positives = 353/417 (84%) Query: 1 MSYDPLDHDFERGMRNRRSVLGDQWVDRSVANATNFNADFQNLITRFAWNEIWGRPGLDH 60 MSYDP+DHDFERGM+NRR +LGD WVDRS+ANAT FNA+FQNLITRFAWNEIWGRPGL+ Sbjct: 1 MSYDPIDHDFERGMQNRRQILGDDWVDRSLANATGFNAEFQNLITRFAWNEIWGRPGLEQ 60 Query: 61 KTRRIIVLAITIALGRWEEFELHVRAGLTADPATRLTPDEMKEVMIQAAVYAGVPAGNTA 120 KTRR+IVLAIT+ALGRWEEFELH RA L D TRLTPDEMKEV++Q A+YAGVPA NTA Sbjct: 61 KTRRVIVLAITMALGRWEEFELHARAALLGDAGTRLTPDEMKEVLMQGAIYAGVPAANTA 120 Query: 121 FTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPALHYTVREPRNGKAPRHT 180 FTHAQKILR++G QIGYA AP APAA+VHPG+GR T S PALHY+VR PR+GKAPRHT Sbjct: 121 FTHAQKILRDIGAQIGYAPAPSAPAAAVHPGIGRAAATDSKPALHYSVRAPRSGKAPRHT 180 Query: 181 VVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLADDAARLLRE 240 VVLSHALG DL MWD LAN LAADCRVIAYDHRGHGSS+ GLY++ADLADDAARLLRE Sbjct: 181 VVLSHALGCDLTMWDSLANLLAADCRVIAYDHRGHGSSDAPAGLYAIADLADDAARLLRE 240 Query: 241 LDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQRIATVRAEG 300 LDSGPVVW+GLSMGGM GQELALRHP LV ALVLAN TS YP ARE WQQRI TVRA+G Sbjct: 241 LDSGPVVWIGLSMGGMTGQELALRHPSLVAALVLANCTSHYPPPAREVWQQRIDTVRAQG 300 Query: 301 IEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVDTAARLGSIR 360 IEVIADAVMGRYFH+ FR+ ATVAR+RQRL TT GY+GCCHAVGTVDT ARL I Sbjct: 301 IEVIADAVMGRYFHDRFRAEHGATVARFRQRLATTGPAGYIGCCHAVGTVDTTARLPQIT 360 Query: 361 VPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQPALFAELVCGFI 417 VPTLVIAGELDQGTP+AMAQ L + I A L V+ ASH+SA EQP FA+ V F+ Sbjct: 361 VPTLVIAGELDQGTPLAMAQTLAEQIPQASLVVLKDASHLSATEQPQAFAQAVVQFM 417 >ref|YP_001022719.1| hydrolase or acyltransferase-like protein [Methylibium petroleiphilum PM1] gb|ABM96484.1| hydrolase or acyltransferase-like protein [Methylibium petroleiphilum PM1] Length = 425 Score = 514 bits (1324), Expect = e-143, Method: Compositional matrix adjust. Identities = 260/425 (61%), Positives = 314/425 (73%), Gaps = 16/425 (3%) Query: 4 DPLDHDFERGMRNRRSVLGDQWVDRSVANATNFNADFQNLITRFAWNEIWGRPGLDHKTR 63 DP DHD ERG+RNRR++LGD WVD+S+A+A F ADFQN ITR+AW+E+WGRPGLD KTR Sbjct: 2 DPYDHDLERGLRNRRALLGDAWVDKSIASANAFTADFQNFITRYAWHEVWGRPGLDAKTR 61 Query: 64 RIIVLAITIALGRWEEFELHVRAGLTA----------DPATRLTPDEMKEVMIQAAVYAG 113 R+IVLAIT ALGRWEEFELH+RA L D T LTPDE+KEV++QAA+YAG Sbjct: 62 RVIVLAITCALGRWEEFELHLRAALVGGSGASLGAGDDAPTALTPDEVKEVLMQAAIYAG 121 Query: 114 VPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASV-HPGVGREGRTASSPALHYTVREPR 172 VPA NT + A K+LRE+G + P PA V H G GR R+A +PALHYTVREPR Sbjct: 122 VPAANTGMSIAVKLLRELGHAL-----PPLPATEVAHTGSGRSFRSAGTPALHYTVREPR 176 Query: 173 NGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLAD 232 + +PR TVVLSHALG D+ +WD LAN LAA+ RVI YDHRGHG SE G Y+MA+LAD Sbjct: 177 SKASPRSTVVLSHALGCDVSLWDALANALAAEHRVICYDHRGHGDSEAPAGPYTMAELAD 236 Query: 233 DAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQR 292 DA RLL ELD+GPVVW+GLS+GGMVGQELALRHP V ALV+AN+++ + +A R AWQQR Sbjct: 237 DAERLLAELDTGPVVWIGLSLGGMVGQELALRHPRRVEALVIANSSAGFDEAGRNAWQQR 296 Query: 293 IATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVDT 352 I V G++ +ADA M R+F FRSAQ ATVAR+R+R+ +T A GYV AV DT Sbjct: 297 IDAVAQGGVDAVADAAMQRWFTTDFRSAQPATVARWRRRVASTSARGYVAASQAVMRHDT 356 Query: 353 AARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQPALFAEL 412 AARL I PTLVIAG LD GTPVAM+QA+ D + ARL V+ A+H+S +EQPA F + Sbjct: 357 AARLPQISAPTLVIAGALDPGTPVAMSQAIADAVPRARLVVLDDAAHLSVLEQPAAFTAV 416 Query: 413 VCGFI 417 V F+ Sbjct: 417 VHDFL 421 >gb|AAX47260.1| hydrolase/acyltransferase [Delftia tsuruhatensis] Length = 269 Score = 258 bits (658), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 135/237 (56%), Positives = 163/237 (68%) Query: 181 VVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLADDAARLLRE 240 +VLSHALG DL WD LA +LA D V+ DHRGHG+S G YSM L +D A LLR Sbjct: 23 IVLSHALGMDLHSWDALAARLARDYTVLRPDHRGHGASPAPAGPYSMDALVEDTAALLRA 82 Query: 241 LDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQRIATVRAEG 300 PVVWVGLSMGGMVG LA+RHP L+R LV+A+ + YPDAAR AW QRIATV AEG Sbjct: 83 RGDAPVVWVGLSMGGMVGLGLAIRHPELLRGLVVAHACAHYPDAARAAWNQRIATVAAEG 142 Query: 301 IEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVDTAARLGSIR 360 I IADAVMGRYFH FR+ A R+ L+ T GY+GCCHAV VD A L SIR Sbjct: 143 IGAIADAVMGRYFHRKFRAEHPDVEAIARRTLLATPLEGYLGCCHAVRDVDWRAGLESIR 202 Query: 361 VPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQPALFAELVCGFI 417 +P LVIAG+ D+G P+A++Q + + GARL V+ GASH+ ++EQP F E+V F+ Sbjct: 203 MPVLVIAGQFDEGVPLALSQTIARAVPGARLQVLQGASHIGSLEQPERFEEMVRAFL 259 >gb|ACO92642.1| putative hydrolase/acyltransferase [Burkholderia sp. JS667] Length = 243 Score = 217 bits (553), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 113/204 (55%), Positives = 138/204 (67%) Query: 214 GHGSSEKADGLYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALV 273 GHG+S G YS+ L +DAA LLR PVVWVGLSMGGMVG LA+RHP L+R LV Sbjct: 30 GHGASPAPAGPYSLDALVEDAAALLRARGGAPVVWVGLSMGGMVGLGLAIRHPELLRGLV 89 Query: 274 LANTTSAYPDAAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLV 333 +A+ + YPDAAR AW QRIATV AEGI ADAVMGRYFH GFR+ R+ L+ Sbjct: 90 VAHACAHYPDAARVAWDQRIATVAAEGIGATADAVMGRYFHRGFRAEHPEVETAARRTLL 149 Query: 334 TTDAVGYVGCCHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAV 393 T GY+GCCHAV VD A L SIR+P LVIAG+ D+G P+A++Q + + GARL V Sbjct: 150 ATPLEGYLGCCHAVRDVDWRAGLESIRMPVLVIAGQFDEGVPLALSQTIARAVPGARLQV 209 Query: 394 IAGASHVSAVEQPALFAELVCGFI 417 + G SH+ +EQP F E+V F+ Sbjct: 210 LQGTSHIGPLEQPERFEEMVRAFL 233 >emb|CBE67933.1| Alpha/beta hydrolase fold [NC10 bacterium 'Dutch sediment'] Length = 262 Score = 217 bits (552), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 109/263 (41%), Positives = 160/263 (60%), Gaps = 6/263 (2%) Query: 158 TASSPALHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGS 217 TA+ ++YT+ +G+ P V +SHALG +L +WD A L+ RV+ YD RGHG Sbjct: 4 TANGIDIYYTI----DGEGP--VVTMSHALGCNLALWDEQAKALSTRYRVLRYDTRGHGR 57 Query: 218 SEKADGLYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANT 277 + YS+ +ADD LL L +VG+SMGGM+GQ AL++P +VR+L+L++T Sbjct: 58 TSAPPEPYSLEQMADDVYGLLNVLGVAQTHFVGISMGGMIGQIFALKYPSMVRSLILSST 117 Query: 278 TSAYPDAAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDA 337 TS YP AAR AW++RI V A+G+E + + + R+F FR + + R + +T Sbjct: 118 TSRYPTAARSAWEERIRAVEAKGMEPLVEPALERWFTAPFRERRQGVMDTVRAMIRSTPP 177 Query: 338 VGYVGCCHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGA 397 GY+GCC+A+ T+D RLG IR P LVIAGE D GTPV MA + + + LA + A Sbjct: 178 QGYIGCCYAIPTIDVTDRLGEIRCPALVIAGENDPGTPVTMAHEICAALPSSELATLPSA 237 Query: 398 SHVSAVEQPALFAELVCGFIDGI 420 SH+ +EQP +F ++ GF+ + Sbjct: 238 SHLCNLEQPEVFNRILLGFLGKV 260 >ref|YP_548688.1| 3-oxoadipate enol-lactonase [Polaromonas sp. JS666] gb|ABE43790.1| 3-oxoadipate enol-lactonase [Polaromonas sp. JS666] Length = 260 Score = 214 bits (546), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 125/261 (47%), Positives = 160/261 (61%), Gaps = 11/261 (4%) Query: 164 LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADG 223 LH+T + G+ P VVLSHALG D+ MWDG+ L + V+ YDHRGHG S+ G Sbjct: 6 LHWT----KEGQGP--VVVLSHALGCDIRMWDGVTALLKSRYTVLRYDHRGHGQSQAPAG 59 Query: 224 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPD 283 YS+ LA+DAA L+RE +GPV +VGLSMGGM Q LA P LV+++V+AN S Y D Sbjct: 60 PYSLDLLAEDAAGLIREQAAGPVHFVGLSMGGMTAQALAASQPQLVKSIVIANAASWYDD 119 Query: 284 AAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRS----AQAATVARYRQRLVTTDAVG 339 AR WQ R+ TV A+G+ IAD M R+F FR+ A VA R +L TDA Sbjct: 120 TARALWQARVQTVLAQGVAAIADGAMQRWFTPEFRADLAGGGAQRVADLRHQLENTDAAA 179 Query: 340 YVGCCHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASH 399 Y C AV ++D A I P LVIAG D+ TP+AM+Q + D I A+L + A+H Sbjct: 180 YAASCEAVASIDFRASNRRIECPALVIAGTRDEATPLAMSQTIADSIPSAQLRTLE-AAH 238 Query: 400 VSAVEQPALFAELVCGFIDGI 420 +SAVEQP FA L+ GF DG+ Sbjct: 239 LSAVEQPEAFARLLEGFFDGV 259 >ref|ZP_06685390.1| 4-carboxymuconolactone decarboxylase [Achromobacter piechaudii ATCC 43553] gb|EFF77787.1| 4-carboxymuconolactone decarboxylase [Achromobacter piechaudii ATCC 43553] Length = 156 Score = 206 bits (523), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 93/138 (67%), Positives = 114/138 (82%) Query: 7 DHDFERGMRNRRSVLGDQWVDRSVANATNFNADFQNLITRFAWNEIWGRPGLDHKTRRII 66 + DFERG+ NRR+VLGD+WV RS+ AT F AD+QN+ITR+AW++IW RPGL HK RR++ Sbjct: 7 EEDFERGLANRRAVLGDEWVQRSLDRATTFTADYQNMITRYAWHDIWSRPGLPHKARRMM 66 Query: 67 VLAITIALGRWEEFELHVRAGLTADPATRLTPDEMKEVMIQAAVYAGVPAGNTAFTHAQK 126 VLA+T++LGRWEEFELHVRA LTA A+RLTPDE+KEV++Q A+YAGVPA NTAF AQ Sbjct: 67 VLAVTLSLGRWEEFELHVRAALTATDASRLTPDELKEVLLQNAIYAGVPAANTAFNLAQN 126 Query: 127 ILREVGEQIGYAVAPQAP 144 ILREV QIGY +A P Sbjct: 127 ILREVAAQIGYDLAAADP 144 >ref|YP_001563021.1| alpha/beta hydrolase fold protein [Delftia acidovorans SPH-1] gb|ABX34636.1| alpha/beta hydrolase fold [Delftia acidovorans SPH-1] Length = 254 Score = 202 bits (513), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 119/246 (48%), Positives = 151/246 (61%), Gaps = 3/246 (1%) Query: 172 RNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLA 231 + G+ P +VLSHALG DL MWDG+A L V+ YDHRGHG S + M DLA Sbjct: 8 QQGQGP--AIVLSHALGCDLQMWDGVAQALEDRFTVVRYDHRGHGRSPAIGEAFDMNDLA 65 Query: 232 DDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQ 291 DDAA ++ L GPV +VGLSMGGM Q LA+RHP LVR++ +AN+ S Y +AAR WQ Sbjct: 66 DDAAGVIEALGQGPVHFVGLSMGGMTAQALAVRHPALVRSITIANSASYYDEAARAGWQA 125 Query: 292 RIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVD 351 RIATV +G+ VIAD M R+F FR+ QAA VA R L A Y C AVG +D Sbjct: 126 RIATVHDQGVAVIADGAMQRWFTPEFRTGQAARVASLRAGLEALAAKPYAAACAAVGGID 185 Query: 352 TAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQPALFAE 411 I P L+IAG D+ TP A+++ + I GA+L + A+H+SAVE P FA+ Sbjct: 186 FRESNRRIACPALLIAGSRDEATPPALSEDMQAQIPGAQLVSLP-AAHLSAVEMPVEFAQ 244 Query: 412 LVCGFI 417 LV F+ Sbjct: 245 LVSDFV 250 >gb|EFV87460.1| carboxymuconolactone decarboxylase [Achromobacter xylosoxidans C54] Length = 150 Score = 200 bits (508), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 90/135 (66%), Positives = 112/135 (82%) Query: 7 DHDFERGMRNRRSVLGDQWVDRSVANATNFNADFQNLITRFAWNEIWGRPGLDHKTRRII 66 + DFERG+ NRR+VLG++WV RS+ AT F AD+QNLITR+AW++IW RPGL HK RR++ Sbjct: 4 EDDFERGLANRRAVLGEEWVQRSLDRATTFTADYQNLITRYAWHDIWSRPGLPHKARRMM 63 Query: 67 VLAITIALGRWEEFELHVRAGLTADPATRLTPDEMKEVMIQAAVYAGVPAGNTAFTHAQK 126 VLA+T++LGRWEEFELHVRA LTA +RL+PDE+KEV++Q A+YAGVPA NTAF AQ Sbjct: 64 VLAVTLSLGRWEEFELHVRAALTAKDESRLSPDELKEVLLQNAIYAGVPAANTAFNLAQG 123 Query: 127 ILREVGEQIGYAVAP 141 ILREV +IGY V P Sbjct: 124 ILREVAGEIGYVVEP 138