BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0251.p (251 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775142.1| esterase_lipase superfamily protein [Herbasp... 501 e-140 ref|YP_002782396.1| hydrolase [Rhodococcus opacus B4] >gi|226243... 129 5e-28 ref|YP_705085.1| hydrolase [Rhodococcus jostii RHA1] >gi|1108216... 123 2e-26 ref|YP_002764504.1| hydrolase [Rhodococcus erythropolis PR4] >gi... 120 2e-25 ref|ZP_08156955.1| hydrolase [Rhodococcus equi ATCC 33707] >gi|3... 119 5e-25 ref|ZP_04388251.1| hydrolase [Rhodococcus erythropolis SK121] >g... 118 9e-25 ref|YP_004008834.1| alpha/beta hydrolase [Rhodococcus equi 103S]... 117 2e-24 ref|YP_001701911.1| alpha/beta fold hydrolase [Mycobacterium abs... 105 5e-21 ref|YP_781899.1| alpha/beta hydrolase fold protein [Rhodopseudom... 105 6e-21 ref|ZP_01551252.1| Alpha/beta hydrolase [Stappia aggregata IAM 1... 103 2e-20 >ref|YP_003775142.1| esterase_lipase superfamily protein [Herbaspirillum seropedicae SmR1] gb|ADJ63234.1| esterase_lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 501 bits (1290), Expect = e-140, Method: Compositional matrix adjust. Identities = 248/251 (98%), Positives = 248/251 (98%) Query: 1 MPFAISDQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQ 60 MPFAISDQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQ Sbjct: 1 MPFAISDQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQ 60 Query: 61 SDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILG 120 SDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILG Sbjct: 61 SDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILG 120 Query: 121 AAHPYEDRSWEVFRGIDGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAAAQQAR 180 AAHPYEDRSWEVFRGIDGTDPEAFIAAFE LLDEAISPQVKMLIRGNDLVALAAAAQQAR Sbjct: 121 AAHPYEDRSWEVFRGIDGTDPEAFIAAFEDLLDEAISPQVKMLIRGNDLVALAAAAQQAR 180 Query: 181 PSQEELLARMDLPCLFFCGDADARHTAVARTAAPLGRAEFVSLPGVTHFGGLMQSALVLP 240 PSQEELLARMDLPCLFFCGDADARHTAVARTAA L RAEFVSLPGVTHFGGLMQSALVLP Sbjct: 181 PSQEELLARMDLPCLFFCGDADARHTAVARTAARLARAEFVSLPGVTHFGGLMQSALVLP 240 Query: 241 PVLDFLGRLPG 251 PVLDFLGRLPG Sbjct: 241 PVLDFLGRLPG 251 >ref|YP_002782396.1| hydrolase [Rhodococcus opacus B4] dbj|BAH53451.1| putative hydrolase [Rhodococcus opacus B4] Length = 277 Score = 129 bits (324), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 98/260 (37%), Positives = 124/260 (47%), Gaps = 19/260 (7%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D +I Y+ G+G PL+L HG S WR FG+ LR YR+I D GHG+SDKPHD Sbjct: 14 DGVRIAYRTVGDGEPLVLVHGTALSHAIWRGFGYVAALRDRYRLILVDLRGHGRSDKPHD 73 Query: 67 SAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILGAAHPYE 126 AY+ D LAVLD +V H GYSLGG V L APERL SLI+G Sbjct: 74 EDAYAMDLLSGDVLAVLDHLEVPSAHVLGYSLGGRVALALAVGAPERLESLIVGGGSSLP 133 Query: 127 -----DRSWEVFRGI------DGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAA 175 DR + F G DG D AF+A + + I + ND ALAA Sbjct: 134 QAGAFDRLF--FPGCIDVLERDGMD--AFLAQWNARRAWPIDAGTRAAFMANDAKALAAY 189 Query: 176 AQQA--RPS-QEELLARMDLPCLFFCGDADARHTA-VARTAAPLGRAEFVSLPGVTHFGG 231 +++ P +++L R+ +P L F G DA R AA + A LPG H Sbjct: 190 MRRSGVEPGVDDDVLRRIAVPTLLFVGSDDAERIGDTRRVAALIPGASLTILPGFDHSTA 249 Query: 232 LMQSALVLPPVLDFLGRLPG 251 + S VL V FL + G Sbjct: 250 VAASTEVLAAVEPFLASVRG 269 >ref|YP_705085.1| hydrolase [Rhodococcus jostii RHA1] gb|ABG96927.1| hydrolase [Rhodococcus jostii RHA1] Length = 267 Score = 123 bits (309), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 95/255 (37%), Positives = 120/255 (47%), Gaps = 19/255 (7%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D +I Y+ G+G PL+L HG S WR FG+ LR YR+I D GHG SDKPHD Sbjct: 14 DGVRIAYKTVGDGEPLVLIHGTALSHAIWRGFGYVAALRDRYRLILVDLRGHGCSDKPHD 73 Query: 67 SAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILGAAHPYE 126 +AY+ D LAVLD + H GYSLGG V L APERL SLI+G Sbjct: 74 ESAYAMDLVSGDVLAVLDHLDLPSAHVLGYSLGGRVALALAVGAPERLESLIVGGGSSRP 133 Query: 127 -----DRSWEVFRGI------DGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAA 175 DR + F G DG D AF+A + + I + ND ALAA Sbjct: 134 QAGAFDRLF--FPGCIDVLERDGMD--AFLAQWNARRSWPIDAGTRAAFMANDAQALAAY 189 Query: 176 AQQA--RPS-QEELLARMDLPCLFFCGDADARHTA-VARTAAPLGRAEFVSLPGVTHFGG 231 +++ P +++L + +P L F G DA R AA + A LPG H Sbjct: 190 MRRSGVEPGVDDDVLRGIAVPTLLFVGSNDAERIGDTQRVAALIPGASLTILPGFDHSTA 249 Query: 232 LMQSALVLPPVLDFL 246 + S VL V FL Sbjct: 250 VAASPEVLAAVEPFL 264 >ref|YP_002764504.1| hydrolase [Rhodococcus erythropolis PR4] dbj|BAH31765.1| putative hydrolase [Rhodococcus erythropolis PR4] Length = 275 Score = 120 bits (302), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 83/254 (32%), Positives = 123/254 (48%), Gaps = 12/254 (4%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D +I YQ+ G+GPPLL+ HG S WR FG+ VL++ YR+I D GHG+SDKPHD Sbjct: 21 DGARIAYQIVGDGPPLLMVHGTALSRSIWRGFGYVKVLQEQYRLILVDMRGHGRSDKPHD 80 Query: 67 SAAYSQHQRCRDALAVLDA-AQVERTHFFGYSLGGWVGYGLVRQAPERLRSLIL--GAAH 123 S +Y+ D L +LD + E H GYS GG L + ER+RSL + G++ Sbjct: 81 SDSYAMDLVVGDLLEILDTESPFEPAHVLGYSFGGRSVMSLTVEHSERVRSLTVGGGSSR 140 Query: 124 PYEDRSWEVF-----RGIDGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAAAQQ 178 P +F ++ E F++ + + + + PQ R ND +ALAA ++ Sbjct: 141 PMTGAFDALFFPGCIDALESGGIEGFLSGWGARRGKPVDPQTATAFRINDALALAAYMRR 200 Query: 179 ARPSQ---EELLARMDLPCLFFCGDAD-ARHTAVARTAAPLGRAEFVSLPGVTHFGGLMQ 234 A Q + +L R+ P L F G D R A+ + + + H + Sbjct: 201 AEKEQGIDDAVLQRITTPALLFVGSRDRERLPDTEHLASLIPDSALTVIEEADHASAVAD 260 Query: 235 SALVLPPVLDFLGR 248 S VL ++ FL R Sbjct: 261 SEAVLRALVPFLSR 274 >ref|ZP_08156955.1| hydrolase [Rhodococcus equi ATCC 33707] gb|EGD21682.1| hydrolase [Rhodococcus equi ATCC 33707] Length = 265 Score = 119 bits (297), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 124/254 (48%), Gaps = 17/254 (6%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D I Y+V G+GPPL+L HG S WR FG+ LR +++I D GHG+SDKPH+ Sbjct: 11 DGVGIAYRVIGDGPPLVLAHGTALSSAIWRAFGYVGSLRDEFQLILPDMRGHGRSDKPHE 70 Query: 67 SAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLIL--GAAHP 124 +++Y+ D LAVLD +R H+ GYS GG VG L AP RLR+L + G++ P Sbjct: 71 ASSYAMDLVVGDILAVLDDLGHDRAHYLGYSFGGRVGLSLATAAPGRLRTLTVGGGSSQP 130 Query: 125 YEDRSWEVF--RGIDGTDPE---AFIAAFESLLDEAISPQVKMLIRGNDLVALAAAAQQA 179 ++F ID D E AF+ + + + P + ND AL A Sbjct: 131 QAGSFDKLFFPGCIDVLDHEGMTAFLDKWNAHRPWPLDPATRAAFDANDTRALVA---YL 187 Query: 180 RPSQEE------LLARMDLPCLFFCGDAD-ARHTAVARTAAPLGRAEFVSLPGVTHFGGL 232 R S+EE L +D+P L F G D R + R A + + +PG H + Sbjct: 188 RRSEEEPGVPDTSLQALDVPALLFVGSEDRPRLSDTKRLAELIPGSRLAVIPGFDHATTV 247 Query: 233 MQSALVLPPVLDFL 246 S VL V FL Sbjct: 248 AASPEVLAVVRPFL 261 >ref|ZP_04388251.1| hydrolase [Rhodococcus erythropolis SK121] gb|EEN84708.1| hydrolase [Rhodococcus erythropolis SK121] Length = 256 Score = 118 bits (295), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 82/254 (32%), Positives = 120/254 (47%), Gaps = 12/254 (4%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D +I YQ+ G+GPPLL+ HG S WR FG+ L++ YR+I D GHG+SDKPHD Sbjct: 2 DGARITYQIVGDGPPLLMVHGTALSRSIWRGFGYVKALQEQYRLILVDMRGHGRSDKPHD 61 Query: 67 SAAYSQHQRCRDALAVLDA-AQVERTHFFGYSLGGWVGYGLVRQAPERLRSLIL--GAAH 123 S +Y+ D L +LD + E H GYS GG L + ER+RSL + G++ Sbjct: 62 SDSYAMDLVVGDLLEILDTESPFEPAHVLGYSFGGRSVMSLTVEHSERVRSLTVGGGSSR 121 Query: 124 PYEDRSWEVF-----RGIDGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAAAQQ 178 P +F ++ E F+ + + + PQ R ND +ALAA ++ Sbjct: 122 PMTGAFDALFFPGCIDVLESGGIEGFLTGWGVRRGKPVDPQTATAFRINDALALAAYMRR 181 Query: 179 ARPSQ---EELLARMDLPCLFFCGDAD-ARHTAVARTAAPLGRAEFVSLPGVTHFGGLMQ 234 A + +L R+ P L F G D R A+ + + + G H + Sbjct: 182 AEKEHGIDDAVLQRITTPALLFVGSRDRERLPDTEHLASLIPDSALTVIEGADHASAVAD 241 Query: 235 SALVLPPVLDFLGR 248 S VL ++ FL R Sbjct: 242 SDAVLRALIPFLSR 255 >ref|YP_004008834.1| alpha/beta hydrolase [Rhodococcus equi 103S] emb|CBH50156.1| putative alpha/beta hydrolase [Rhodococcus equi 103S] Length = 265 Score = 117 bits (292), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 89/254 (35%), Positives = 124/254 (48%), Gaps = 17/254 (6%) Query: 7 DQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSDKPHD 66 D I Y+V G+GPPL+L HG S WR FG+ LR +++I D GHG+S+KPH+ Sbjct: 11 DGVGIAYRVIGDGPPLVLAHGTALSSAIWRAFGYVGSLRDEFQLILPDMRGHGRSEKPHE 70 Query: 67 SAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLIL--GAAHP 124 +++Y+ D LAVLD +R H+ GYS GG VG L AP RLR+L + G++ P Sbjct: 71 ASSYAMDLVVGDILAVLDDLGHDRAHYLGYSFGGRVGLSLATAAPGRLRTLTVGGGSSRP 130 Query: 125 YEDRSWEVF--RGIDGTDPE---AFIAAFESLLDEAISPQVKMLIRGNDLVALAAAAQQA 179 ++F ID D E AF+ + + + P + ND AL A Sbjct: 131 QAGSFDKLFFPGCIDVLDHEGMTAFLDKWNAHRPWPLDPATRAAFDANDTRALVA---YL 187 Query: 180 RPSQEE------LLARMDLPCLFFCGDAD-ARHTAVARTAAPLGRAEFVSLPGVTHFGGL 232 R S+EE L +D+P L F G D R + R A + + +PG H + Sbjct: 188 RRSEEEPGVPDTSLQALDVPTLLFVGSEDRPRLSDTKRLAELIPGSRLAVIPGFDHATTV 247 Query: 233 MQSALVLPPVLDFL 246 S VL V FL Sbjct: 248 AASPEVLAVVRPFL 261 >ref|YP_001701911.1| alpha/beta fold hydrolase [Mycobacterium abscessus ATCC 19977] emb|CAM61257.1| Putative hydrolase, alpha/beta fold [Mycobacterium abscessus] Length = 292 Score = 105 bits (263), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 79/237 (33%), Positives = 112/237 (47%), Gaps = 15/237 (6%) Query: 7 DQRKIHYQVEGEGPP----LLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQSD 62 D + Y+ G P ++L HG + W G+ L Q YRV + D GHG S+ Sbjct: 23 DGAAVAYRTYGRSRPGVVDVVLVHGSLQNSAVWSKHGYIAQLSQQYRVTTIDVRGHGASE 82 Query: 63 KPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLIL-GA 121 KP Y+ RD AVLD QV + H+ GYSLGG +G L APERL SL++ G+ Sbjct: 83 KPDHPGGYAIASSARDVCAVLDHLQVHQAHYLGYSLGGRIGLTLAATAPERLTSLVVAGS 142 Query: 122 AHPYEDRSWEVF------RGIDGTDPEAFIAAFESLLDEAISPQVKMLIRGNDLVALAAA 175 +H + + +V R ++ + AFI +ES + ++ + I LAA Sbjct: 143 SHRPQRGAVDVLIFPNAIRVVEESGLPAFIDGWESHRGQRMASGFRATIEALGQRGLAAL 202 Query: 176 AQQARPS---QEELLARMDLPCLFFCGDADARHTAVARTAA-PLGRAEFVSLPGVTH 228 +Q EE L +++ P LFF G D A +R AA + RA F L G H Sbjct: 203 LRQWDDEPGVTEEALLQIETPTLFFAGSEDPMRLAESREAAVRMPRAYFALLEGCNH 259 >ref|YP_781899.1| alpha/beta hydrolase fold protein [Rhodopseudomonas palustris BisA53] gb|ABJ06919.1| alpha/beta hydrolase fold [Rhodopseudomonas palustris BisA53] Length = 263 Score = 105 bits (262), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 55/146 (37%), Positives = 77/146 (52%), Gaps = 10/146 (6%) Query: 1 MPFAISDQRKIHYQVEGEGPPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGHGQ 60 MPFA +IHY EG+GP ++L HG +W+ G D L+ +R++ D+LGHG Sbjct: 1 MPFATHRGLRIHYTSEGDGPLVVLLHGLLFDAASWKATGIVDYLQDRFRLVCIDSLGHGD 60 Query: 61 SDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLILG 120 SDKP D+ Y Q QR D +AVLD R H G+ +GGW+ L + P+ L SL++G Sbjct: 61 SDKPLDAKLYVQPQRAGDVVAVLDELGYARAHVIGHGMGGWLAVRLAKFFPQHLSSLVIG 120 Query: 121 AAHPYEDRSWEVFRGI--DGTDPEAF 144 W + G+ D P F Sbjct: 121 G--------WNLVSGVAPDHAGPMTF 138 >ref|ZP_01551252.1| Alpha/beta hydrolase [Stappia aggregata IAM 12614] gb|EAV40184.1| Alpha/beta hydrolase [Stappia aggregata IAM 12614] Length = 251 Score = 103 bits (258), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 80/238 (33%), Positives = 115/238 (48%), Gaps = 17/238 (7%) Query: 1 MPFAISDQRKIHYQVEGEGPPLLLHHGFTSSLEA-WRFFGFTDVLR-QHYRVISFDALGH 58 MP D +I YQ +GEG P+LL HGF S+ + W++ G+ D+L RVI+ D GH Sbjct: 1 MPQFEFDGVRIAYQDQGEGDPILLIHGFASNKQVNWQYPGWVDLLVGDGRRVITIDNRGH 60 Query: 59 GQSDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLI 118 G S K +D AAY DA +LD +ER GYS+G + L P+R+R I Sbjct: 61 GDSQKFYDPAAYGAPVMAEDAKRLLDHLNIERADVMGYSMGARISAFLTLNHPDRVRRAI 120 Query: 119 LGAAHPYEDRSWEVFRGIDGTDPEAFIAAFESLLDEAISPQVKMLIR------GNDLVAL 172 + + + G+ DPE AA E+ + IS + R G+D AL Sbjct: 121 ------FSGLGYGMISGV--GDPEPIAAALEADRIQDISDRTGRAFRAFAEQTGSDRRAL 172 Query: 173 AAAAQQARPS-QEELLARMDLPCLFFCGDADARHTAVARTAAPLGRAEFVSLPGVTHF 229 AA + +R EE +AR++ P L G D + + AA + AE + +PG H Sbjct: 173 AACMRSSRQKISEEDVARIERPVLIAVGTKDDIAGSPHKLAALIPHAEVLEIPGRDHM 230