BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0162.p (336 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775057.1| transmembrane protein [Herbaspirillum serope... 156 4e-36 ref|YP_004753696.1| putative transmembrane protein [Collimonas f... 105 1e-20 ref|ZP_08272651.1| C-5 sterol desaturase [Oxalobacteraceae bacte... 102 1e-19 ref|YP_002943546.1| fatty acid hydroxylase [Variovorax paradoxus... 80 4e-13 ref|YP_004686064.1| C-5 sterol desaturase Erg [Cupriavidus necat... 74 2e-11 ref|ZP_06685463.1| conserved hypothetical protein [Achromobacter... 74 3e-11 ref|YP_003745974.1| hypothetical protein RCFBP_20175 [Ralstonia ... 74 3e-11 ref|YP_726803.1| C-5 sterol desaturase [Ralstonia eutropha H16] ... 73 5e-11 ref|YP_001021270.1| hypothetical protein Mpe_A2079 [Methylibium ... 73 5e-11 gb|AEG69406.1| sterol desaturase protein [Ralstonia solanacearum... 73 6e-11 >ref|YP_003775057.1| transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ63149.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 156 bits (395), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 76/76 (100%), Positives = 76/76 (100%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP Sbjct: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 Query: 61 LESAIPVHPITDPRAR 76 LESAIPVHPITDPRAR Sbjct: 61 LESAIPVHPITDPRAR 76 >ref|YP_004753696.1| putative transmembrane protein [Collimonas fungivorans Ter331] gb|AEK62873.1| putative transmembrane protein [Collimonas fungivorans Ter331] Length = 328 Score = 105 bits (262), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 59/133 (44%), Positives = 79/133 (59%), Gaps = 14/133 (10%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M T+ D FAA Q WLFQ++VQPL+F G E++E+AF GTE+ L+G+CELV+LFLILRP Sbjct: 1 MTNTLIDWFAAAQAWLFQSVVQPLMFHAGFSEYLEDAFDGTEWFLIGVCELVVLFLILRP 60 Query: 61 LESAIPVHPITDPRARARARARALERFHLHRAAAAG------RDPHRGLPAARSGAVLPV 114 LE+ IPVH +D RAR + L+R A DP A++ +L + Sbjct: 61 LEALIPVHSFSDRRARWIDFLYTV----LYRLGAFSVFVFFLVDP----LMAKATELLHL 112 Query: 115 RGAAPGRLGQLQP 127 +G P L L P Sbjct: 113 QGINPFNLENLLP 125 >ref|ZP_08272651.1| C-5 sterol desaturase [Oxalobacteraceae bacterium IMCC9480] gb|EGF33874.1| C-5 sterol desaturase [Oxalobacteraceae bacterium IMCC9480] Length = 327 Score = 102 bits (253), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 42/76 (55%), Positives = 61/76 (80%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 MI + DLF VQGWLFQ++++P ++ G+GEF E+AF GTE++L+G+CE+V+LF++LRP Sbjct: 1 MIAYLVDLFGQVQGWLFQSVIEPFIYLTGLGEFTEQAFEGTEWLLIGVCEIVLLFVLLRP 60 Query: 61 LESAIPVHPITDPRAR 76 LE+ +P ITD RAR Sbjct: 61 LEALLPAQVITDKRAR 76 >ref|YP_002943546.1| fatty acid hydroxylase [Variovorax paradoxus S110] gb|ACS18280.1| fatty acid hydroxylase [Variovorax paradoxus S110] Length = 324 Score = 80.5 bits (197), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 42/94 (44%), Positives = 61/94 (64%), Gaps = 1/94 (1%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 MI ++D F+A+QGW F+TLVQPLVF +G+G + E+AF T ++LVGL ++V+L ++ P Sbjct: 1 MIDRLTDAFSALQGWFFETLVQPLVFAVGLGGWTEDAFDATGWLLVGLIQVVVLLAVIGP 60 Query: 61 LESAIPVHPITDPRA-RARARARALERFHLHRAA 93 L+ V P+TD RA R + R L R A Sbjct: 61 LQRWRAVEPVTDRRAIRIDVLYTLIHRLGLFRLA 94 >ref|YP_004686064.1| C-5 sterol desaturase Erg [Cupriavidus necator N-1] gb|AEI77583.1| C-5 sterol desaturase Erg [Cupriavidus necator N-1] Length = 324 Score = 74.3 bits (181), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 41/94 (43%), Positives = 59/94 (62%), Gaps = 1/94 (1%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M +T++ V+G LFQ +V P+V+ +G G + EEAFTGTE++LVGL ++V++ LIL P Sbjct: 1 MWETLNGWIGQVEGTLFQDVVLPVVYDLGFGRYAEEAFTGTEWLLVGLVQIVVMALILGP 60 Query: 61 LESAIPVHPITDPRA-RARARARALERFHLHRAA 93 LE PV P++D A R + R L R A Sbjct: 61 LEKWRPVEPVSDRAAIRTDIFYTVIHRLGLFRLA 94 >ref|ZP_06685463.1| conserved hypothetical protein [Achromobacter piechaudii ATCC 43553] gb|EFF77610.1| conserved hypothetical protein [Achromobacter piechaudii ATCC 43553] Length = 335 Score = 74.3 bits (181), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 32/73 (43%), Positives = 49/73 (67%) Query: 2 IQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRPL 61 + T+S F A Q WLF+TL+QP +F +GM F+E+ + T ++LVGL ++ +L L+ PL Sbjct: 1 MDTLSQFFGACQQWLFETLIQPALFHLGMSNFIEDGYDATMWLLVGLVQIAVLVLVFGPL 60 Query: 62 ESAIPVHPITDPR 74 + PV P+TD R Sbjct: 61 QRLRPVEPVTDRR 73 >ref|YP_003745974.1| hypothetical protein RCFBP_20175 [Ralstonia solanacearum CFBP2957] emb|CBJ43367.1| conserved membrane protein of unknown function [Ralstonia solanacearum CFBP2957] Length = 324 Score = 73.9 bits (180), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 37/79 (46%), Positives = 54/79 (68%), Gaps = 1/79 (1%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M+ I+D F Q LFQ +V PL + IGMG +VE+A+ GTE++L+GL ++ ++ L+LRP Sbjct: 1 MLDWIADTFTQAQDLLFQHVVMPLTYAIGMGGYVEDAYPGTEWLLMGLVQITVMLLVLRP 60 Query: 61 LESAIPVHPITDPRARARA 79 LE PV P+ D R+ RA Sbjct: 61 LERWRPVEPLQD-RSAVRA 78 >ref|YP_726803.1| C-5 sterol desaturase [Ralstonia eutropha H16] emb|CAJ93435.1| C-5 sterol desaturase [Ralstonia eutropha H16] Length = 324 Score = 73.2 bits (178), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 40/94 (42%), Positives = 58/94 (61%), Gaps = 1/94 (1%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M +T++ V+G LFQ +V P+V+ +G G + EEAFTGTE++LVGL ++V++ LIL P Sbjct: 1 MWETLNGWIGQVEGTLFQDVVLPVVYDLGFGRYAEEAFTGTEWLLVGLVQIVVMALILGP 60 Query: 61 LESAIPVHPITD-PRARARARARALERFHLHRAA 93 LE PV P++D R + R L R A Sbjct: 61 LEKWRPVEPVSDRAGIRTDIFYTVIHRLGLFRLA 94 >ref|YP_001021270.1| hypothetical protein Mpe_A2079 [Methylibium petroleiphilum PM1] gb|ABM95035.1| putative membrane protein [Methylibium petroleiphilum PM1] Length = 324 Score = 73.2 bits (178), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 50/125 (40%), Positives = 68/125 (54%), Gaps = 6/125 (4%) Query: 2 IQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRPL 61 + T++DLFA Q LF+ +VQPL+F +G G +E+ F T ++LVGL +L ++ ++ PL Sbjct: 1 MDTLTDLFAQAQQALFEAVVQPLLFSLGFGNLLEDGFDATGWLLVGLLQLTVMLAVIAPL 60 Query: 62 ESAIPVHPITDPRA-RARARARALERFHLHRAA-AAGRDPHRGLPAARSGAVLPVRGAAP 119 E PV P+ D RA R L R L R A DP L A GA L VRG Sbjct: 61 ERWRPVEPVVDRRAVRVDVLYTLLHRLGLFRVALFFAVDP---LWDALFGA-LSVRGYGG 116 Query: 120 GRLGQ 124 +L Q Sbjct: 117 WQLDQ 121 >gb|AEG69406.1| sterol desaturase protein [Ralstonia solanacearum Po82] Length = 324 Score = 73.2 bits (178), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 37/79 (46%), Positives = 53/79 (67%), Gaps = 1/79 (1%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M+ I+D F Q LFQ +V PL + IGMG +VE+A+ GTE++L+GL ++ ++ L+LRP Sbjct: 1 MLDWIADTFTQAQDLLFQHVVMPLTYAIGMGGYVEDAYPGTEWLLMGLVQITVMLLVLRP 60 Query: 61 LESAIPVHPITDPRARARA 79 LE PV P+ D R RA Sbjct: 61 LERWRPVEPLQD-RGAVRA 78