BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0250.p (129 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774694.1| transporter family protein [Herbaspirillum s... 243 7e-63 ref|ZP_03399923.1| hypothetical protein PSPTOT1_3183 [Pseudomona... 158 3e-37 ref|ZP_07250583.1| hypothetical protein PsyrptK_03562 [Pseudomon... 157 5e-37 ref|YP_003675664.1| hypothetical protein M301_2733 [Methylotener... 149 2e-34 ref|ZP_08018205.1| membrane protein [Lautropia mirabilis ATCC 51... 142 2e-32 ref|YP_003049347.1| hypothetical protein Mmol_1917 [Methylotener... 136 9e-31 ref|XP_002536672.1| conserved hypothetical protein [Ricinus comm... 119 1e-25 ref|ZP_03629755.1| protein of unknown function DUF6 transmembran... 94 1e-17 ref|YP_075940.1| hypothetical protein STH2111 [Symbiobacterium t... 92 2e-17 ref|ZP_03757606.1| hypothetical protein CLOSTASPAR_01612 [Clostr... 92 4e-17 >ref|YP_003774694.1| transporter family protein [Herbaspirillum seropedicae SmR1] gb|ADJ62786.1| bacterial/archaeal transporter family protein [Herbaspirillum seropedicae SmR1] Length = 140 Score = 243 bits (620), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 127/129 (98%), Positives = 128/129 (99%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVP SQLDMLTWKNFFWLG Sbjct: 12 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPTSQLDMLTWKNFFWLG 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA Sbjct: 72 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 131 Query: 121 GGLVVIARG 129 GGL+VIARG Sbjct: 132 GGLLVIARG 140 >ref|ZP_03399923.1| hypothetical protein PSPTOT1_3183 [Pseudomonas syringae pv. tomato T1] ref|ZP_07230933.1| hypothetical protein PsyrptM_07777 [Pseudomonas syringae pv. tomato Max13] gb|EEB57027.1| hypothetical protein PSPTOT1_3183 [Pseudomonas syringae pv. tomato T1] Length = 137 Score = 158 bits (399), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 86/128 (67%), Positives = 101/128 (78%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAGFTSVIAK+GL GIS +LGL IRT FV FVLLFAA VP ++ +T +N WLG Sbjct: 8 MFFAGFTSVIAKMGLAGISGELGLTIRTLFVCAFVLLFAAWAVPTQEVANITSRNLSWLG 67 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 LSGVTTA SW+FYYKA+K+GDV+TVALIDKGSVVVA+LLA +L E IT +AGA LI Sbjct: 68 LSGVTTALSWIFYYKALKVGDVATVALIDKGSVVVAMLLAGLVLREVITLRMIAGAGLIV 127 Query: 121 GGLVVIAR 128 GL+VIA+ Sbjct: 128 AGLLVIAK 135 >ref|ZP_07250583.1| hypothetical protein PsyrptK_03562 [Pseudomonas syringae pv. tomato K40] Length = 141 Score = 157 bits (397), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 86/128 (67%), Positives = 101/128 (78%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAGFTSVIAK+GL GIS +LGL IRT FV FVLLFAA VP ++ +T +N WLG Sbjct: 12 MFFAGFTSVIAKMGLAGISGELGLTIRTLFVCAFVLLFAAWAVPTQEVANITSRNLSWLG 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 LSGVTTA SW+FYYKA+K+GDV+TVALIDKGSVVVA+LLA +L E IT +AGA LI Sbjct: 72 LSGVTTALSWIFYYKALKVGDVATVALIDKGSVVVAMLLAGLVLREVITLRMIAGAGLIV 131 Query: 121 GGLVVIAR 128 GL+VIA+ Sbjct: 132 AGLLVIAK 139 >ref|YP_003675664.1| hypothetical protein M301_2733 [Methylotenera versatilis 301] gb|ADI31087.1| protein of unknown function DUF6 transmembrane [Methylotenera versatilis 301] Length = 140 Score = 149 bits (375), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 79/128 (61%), Positives = 98/128 (76%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAGFTSV+AK GL GISA+LGL +RT FV +FVL+FAA V +++ L N+ WL Sbjct: 12 MFFAGFTSVVAKQGLVGISAELGLTVRTIFVCIFVLVFAAMAVDPAEVRSLQKVNYVWLA 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 S +TTA SW+FYYKA+K+GDV+TVALIDKGSVV+A+LLA W+L EAIT + GA LI Sbjct: 72 FSAITTAGSWIFYYKALKVGDVATVALIDKGSVVIAILLAWWLLKEAITLRLILGALLIV 131 Query: 121 GGLVVIAR 128 GL VIA+ Sbjct: 132 AGLFVIAK 139 >ref|ZP_08018205.1| membrane protein [Lautropia mirabilis ATCC 51599] gb|EFV95093.1| membrane protein [Lautropia mirabilis ATCC 51599] Length = 140 Score = 142 bits (357), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 77/128 (60%), Positives = 95/128 (74%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAGFTSVIAK+G+ GISA+LGL++RT FV FV +FA V ++L L ++ WL Sbjct: 12 MLFAGFTSVIAKMGMEGISAELGLSVRTLFVCGFVFVFALMFVDPAELPRLNGRSLMWLC 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 +SG TTA SW+FYYKAIK G V+TVALIDKGSVVVA++LA +L EAITP GA LI Sbjct: 72 ISGATTALSWIFYYKAIKEGQVATVALIDKGSVVVAMILAWILLREAITPRMAIGATLIV 131 Query: 121 GGLVVIAR 128 GL+V+ R Sbjct: 132 SGLLVMVR 139 >ref|YP_003049347.1| hypothetical protein Mmol_1917 [Methylotenera mobilis JLW8] gb|ACT48820.1| protein of unknown function DUF6 transmembrane [Methylotenera mobilis JLW8] Length = 140 Score = 136 bits (343), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 71/128 (55%), Positives = 93/128 (72%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAGFT+VIAK GLT ISA+LGL +RT FV F++ F + +++ L ++ WL Sbjct: 12 MLFAGFTAVIAKQGLTDISAELGLTVRTLFVCAFIIFFGLLSIAPAEIKNLQKHHYIWLA 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 LS +TTA SW+FYYKA+K+GDV+TVALIDKGSVVV VLLA + EAIT + GA LI Sbjct: 72 LSALTTAGSWIFYYKALKMGDVATVALIDKGSVVVTVLLAWLLFKEAITLRMMIGAILIV 131 Query: 121 GGLVVIAR 128 G++V++R Sbjct: 132 AGVIVLSR 139 >ref|XP_002536672.1| conserved hypothetical protein [Ricinus communis] gb|EEF25711.1| conserved hypothetical protein [Ricinus communis] Length = 425 Score = 119 bits (298), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 65/124 (52%), Positives = 86/124 (69%) Query: 1 MAFAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLG 60 M FAG T+VIAK GL GIS ++G+ +R+ +V +FV FA VPA++ L +N FWL Sbjct: 12 MLFAGVTTVIAKHGLNGISGEMGVVLRSMYVALFVGAFAVLFVPAAEWQTLQRENLFWLA 71 Query: 61 LSGVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIA 120 +S TTA SW+++YKA+K GD++TV LIDKGS V+A+LLA I EAIT GA LI Sbjct: 72 ISSFTTAVSWIYFYKALKEGDIATVTLIDKGSTVIAILLAWLIFKEAITWRIALGAGLII 131 Query: 121 GGLV 124 GL+ Sbjct: 132 AGLL 135 >ref|ZP_03629755.1| protein of unknown function DUF6 transmembrane [bacterium Ellin514] gb|EEF59922.1| protein of unknown function DUF6 transmembrane [bacterium Ellin514] Length = 139 Score = 93.6 bits (231), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 52/127 (40%), Positives = 82/127 (64%) Query: 3 FAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLGLS 62 FAG T+++AK+G++GI ++L A+RT V +F A + P + L L+ + + +LGLS Sbjct: 13 FAGITAILAKIGVSGIDSNLATAVRTTVVLIFTWGIALSTSPIASLGGLSRRTWLFLGLS 72 Query: 63 GVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIAGG 122 G+ T SW+ Y++A++LG S VA +DK SVV A++LA IL+E +T + G I G Sbjct: 73 GLATGLSWLCYFRALQLGQASRVAPVDKLSVVFAIILAVLILHERLTWHQCVGGVFIVIG 132 Query: 123 LVVIARG 129 V++A G Sbjct: 133 AVIVAMG 139 >ref|YP_075940.1| hypothetical protein STH2111 [Symbiobacterium thermophilum IAM 14863] dbj|BAD41096.1| conserved hypothetical protein [Symbiobacterium thermophilum IAM 14863] Length = 138 Score = 92.4 bits (228), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 56/123 (45%), Positives = 75/123 (60%), Gaps = 5/123 (4%) Query: 3 FAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLGLS 62 FA TS++AK+G+ G+ A+L AIRT + A+ + LT N+ +L LS Sbjct: 12 FAALTSILAKIGIKGVDANLATAIRTTVILFLSWGIVFAIGEHHAIRSLTRHNWTFLILS 71 Query: 63 GVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIAGG 122 G+ T SW+FYYKAI LGDVS VA IDK S+V+ ++L+ IL E TP L+AGG Sbjct: 72 GLATGLSWLFYYKAIALGDVSKVAPIDKSSIVLTLILSYLILGEPFTP-----KTLLAGG 126 Query: 123 LVV 125 LVV Sbjct: 127 LVV 129 >ref|ZP_03757606.1| hypothetical protein CLOSTASPAR_01612 [Clostridium asparagiforme DSM 15981] gb|EEG56305.1| hypothetical protein CLOSTASPAR_01612 [Clostridium asparagiforme DSM 15981] Length = 285 Score = 91.7 bits (226), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 50/126 (39%), Positives = 81/126 (64%) Query: 3 FAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLGLS 62 FAG T+V++K+G+ + +D+G A+RT V VF + + ++ + F +L LS Sbjct: 12 FAGITAVLSKIGMQDMDSDVGTAVRTGVVLVFSWMMVFLTGSIGTVGEISGRTFLFLVLS 71 Query: 63 GVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAITPAKLAGAALIAGG 122 G+ T ASW+FY++A++LG+V+ VA IDK S V+ +LLA IL EA++P K+ LI G Sbjct: 72 GLATGASWLFYFRALQLGNVNKVAPIDKSSTVLTMLLAFLILGEALSPLKVVCMILIGAG 131 Query: 123 LVVIAR 128 +++ R Sbjct: 132 TLLMIR 137 Score = 73.6 bits (179), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 39/106 (36%), Positives = 61/106 (57%) Query: 3 FAGFTSVIAKLGLTGISADLGLAIRTCFVFVFVLLFAAAVVPASQLDMLTWKNFFWLGLS 62 FA T+++ K+G+ G+ ++LG AIRTC V V L ++ + KN +L LS Sbjct: 160 FASLTAILGKIGIQGVESNLGTAIRTCVVLVMAWLLVFVKGKQGEVRRINGKNGLFLVLS 219 Query: 63 GVTTAASWVFYYKAIKLGDVSTVALIDKGSVVVAVLLACWILNEAI 108 G+ T SW+ YY+A++ G S V IDK S++V +L + L E + Sbjct: 220 GLATGGSWLCYYRALQDGQASVVVPIDKLSILVTILFSRLFLGERL 265