BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0005.p (564 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum serop... 1135 0.0 ref|YP_746170.1| pyruvate dehydrogenase [Granulibacter bethesden... 467 e-129 ref|YP_001424132.2| pyruvate dehydrogenase [cytochrome] [Coxiell... 467 e-129 ref|YP_003114119.1| thiamine pyrophosphate protein TPP binding d... 451 e-124 ref|ZP_06914413.1| pyruvate oxidase [Streptomyces sviceus ATCC 2... 448 e-123 ref|YP_003511560.1| thiamine pyrophosphate protein TPP binding d... 448 e-123 ref|YP_001990540.1| pyruvate dehydrogenase [Rhodopseudomonas pal... 447 e-123 ref|YP_483825.1| pyruvate dehydrogenase [Rhodopseudomonas palust... 442 e-122 ref|NP_822130.1| pyruvate dehydrogenase/oxidase FAD and thiamine... 442 e-122 ref|YP_004859336.1| thiamine pyrophosphate TPP-binding domain-co... 442 e-122 >ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ63768.1| pyruvate dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 564 Score = 1135 bits (2936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 559/564 (99%), Positives = 560/564 (99%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAA Q Sbjct: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL Sbjct: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE Sbjct: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL Sbjct: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLA 240 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 YDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR Sbjct: 241 SYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK Sbjct: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI Sbjct: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMP+F Sbjct: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF 480 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH Sbjct: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 Query: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 IKMEQIWRFGLAKVRESLIAMKGG Sbjct: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 >ref|YP_746170.1| pyruvate dehydrogenase [Granulibacter bethesdensis CGDNIH1] gb|ABI63247.1| pyruvate oxidase [Granulibacter bethesdensis CGDNIH1] Length = 577 Score = 467 bits (1201), Expect = e-129, Method: Compositional matrix adjust. Identities = 242/554 (43%), Positives = 344/554 (62%), Gaps = 3/554 (0%) Query: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQAKL 63 TVA+IL +TL IGVR+++GVVGD+LN +T+A+RR ++WI VRHEE AA AA ++L Sbjct: 5 TVAEILAETLQVIGVRRVYGVVGDSLNAITEALRRRPDMDWIHVRHEESAAFAAGADSQL 64 Query: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 TG++AVC G+ GPG HL+ GLY+A++ P+LAI+ +P A G++Y QE PE LFR+ Sbjct: 65 TGQIAVCAGSCGPGNLHLINGLYDAQRSRTPMLAIAAHIPTAEIGSNYFQETRPEALFRE 124 Query: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHT-LRERT 182 + Y + ++ PDQ P + AI A +GVA + IP DV V S T L Sbjct: 125 CSVYCETVSDPDQMPRTLNTAIRAAIGHQGVAVVVIPGDVAMKTVPSPVVSTKTALCPEP 184 Query: 183 PLSPPAAD-IQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLVG 241 P++ P+ + + A L+ AAG V + G GCRGA DEV+ALA L+AP++H GK+ + Sbjct: 185 PVTSPSEESLNALTALLEAAGKVTLMCGRGCRGAHDEVVALAEHLKAPIVHALGGKEWIE 244 Query: 242 YDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGRR 301 YD+P+ +G GLIG + G A+ + +++LMLG+D+PY +F P ++K+ Q+D R LGRR Sbjct: 245 YDNPYDVGMTGLIGFSSGYYAMESCDMLLMLGTDFPYRQFFPEEAKIAQVDLRPENLGRR 304 Query: 302 APVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIKP 361 + LG+ G V+ + LL R++ K D A L H + LD A+ K I P Sbjct: 305 TRIDLGVVGDVSATISALLPRLKAKQDTAHLDTCRQHYLKARKGLDDLADGKAGRPPIHP 364 Query: 362 QSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGIQ 421 Q VAR IS+ A DDA F D G T+W +++ G +R+L SF + ++ +L QA G Q Sbjct: 365 QHVARLISEHASDDAVFACDVGTPTIWAARYLKMNGRRRLLGSFAHGSMANALPQAIGAQ 424 Query: 422 ALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSFE 481 ERQVV GDGGF ML+G+F+T V+HRLPVK+VVFNN G V +EM+ AG+ Sbjct: 425 VSAPERQVVSMSGDGGFAMLMGDFLTLVQHRLPVKIVVFNNGTLGFVEMEMKAAGLLE-S 483 Query: 482 GSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPHI 541 G NPDFAA ARACG V P +L+ ++ +L PGPA+LD ++ EL + P + Sbjct: 484 GVALDNPDFAAMARACGVYARRVEDPGKLDEAVRDVLNHPGPALLDAVVNRQELSMPPKV 543 Query: 542 KMEQIWRFGLAKVR 555 +EQ+ F L +R Sbjct: 544 SIEQVKGFSLYMLR 557 >ref|YP_001424132.2| pyruvate dehydrogenase [cytochrome] [Coxiella burnetii Dugway 5J108-111] gb|ABS76858.2| pyruvate dehydrogenase (cytochrome) [Coxiella burnetii Dugway 5J108-111] Length = 563 Score = 467 bits (1201), Expect = e-129, Method: Compositional matrix adjust. Identities = 240/558 (43%), Positives = 346/558 (62%), Gaps = 4/558 (0%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKR--IEWIGVRHEEGAALAAV 58 M V ++L+D L RQIFGV GDA+NPL +A+R DKR EWI VRHEE A AA Sbjct: 2 MKINVWEMLLDILDGCSNRQIFGVFGDAINPLANALRTDKRKRFEWIAVRHEETGAFAAG 61 Query: 59 CQAKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPE 118 QAKLTG+L +C GT GPGA HL+ GLY+A+ DHAPVLA++G VP GTDY QE + + Sbjct: 62 AQAKLTGKLGLCAGTVGPGAIHLLNGLYDAKMDHAPVLALTGQVPRDEIGTDYHQEVNLD 121 Query: 119 LLFRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTL 178 LF DV+AY++ + P+Q P + A+ A A++GVAHL+IP DV +K Sbjct: 122 RLFDDVSAYNESLIVPEQMPRLGLLAVQTAIARQGVAHLSIPTDVGPSKEINWSLDHPVF 181 Query: 179 RERTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKD 238 ++ P D++ A +N + V IF G G RGA E+L LA KL++PV+H +GK Sbjct: 182 QKYQQPVPAPQDLENIATALNRSDKVTIFIGWGARGATQEILDLAQKLKSPVIHALKGKA 241 Query: 239 LVGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVL 298 ++ ++P+W GGVG++G G +A+ + E++LMLG+D+PY +F P +IQID L Sbjct: 242 VIANENPYWAGGVGMLGSTCGMEAMESCELLLMLGTDFPYRQFYPKGKTIIQIDTLPEHL 301 Query: 299 GRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKK 358 G+R V +G+ G V +V LL V+ K L++ R+ W ++ A I+ Sbjct: 302 GKRCNVKMGVVGDVKASVTALLPMVKEKKSDKHLKRTQRKRKEWEERMLKRA-IRPLKNS 360 Query: 359 IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQAN 418 I+PQ+VA I+ A D+A F DTG +W I +G + +ASFN A++ + QA Sbjct: 361 IRPQAVAHLINHYASDNAVFTADTGETIIWMARQIEMKGERDFIASFNLASMANAFAQAI 420 Query: 419 GIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMP 478 G+QALDR RQVV GDGGFTML+G+F+T+V H+LP+K VFNN + GLV +EME +G P Sbjct: 421 GVQALDRSRQVVAMCGDGGFTMLMGDFITAVSHQLPIKAFVFNNGKLGLVKMEMEASGYP 480 Query: 479 SFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVM 538 + G NP+ A A+A GG+G V + ++L+ +++ GP ++DV +P+EL V Sbjct: 481 EW-GVSLHNPNLADCAKAMGGEGIRVESHDQLKNAVKRAFEIDGPVLVDVLTNPNELIVP 539 Query: 539 PHIKMEQIWRFGLAKVRE 556 P +K + W F ++K++E Sbjct: 540 PKLKPAEAWGFSISKMKE 557 >ref|YP_003114119.1| thiamine pyrophosphate protein TPP binding domain-containing protein [Catenulispora acidiphila DSM 44928] gb|ACU72278.1| thiamine pyrophosphate protein TPP binding domain protein [Catenulispora acidiphila DSM 44928] Length = 576 Score = 451 bits (1159), Expect = e-124, Method: Compositional matrix adjust. Identities = 242/571 (42%), Positives = 350/571 (61%), Gaps = 16/571 (2%) Query: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQAKL 63 TVA+ ++ L ++GVR ++GVVGDALNP+TDAIRR++RIEWIG RHEE AA AA QA+L Sbjct: 3 TVAEQIVTALADLGVRTVWGVVGDALNPVTDAIRREERIEWIGTRHEEAAAFAASAQAQL 62 Query: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 +G + VC GT GPG+ HL+ GLY+A+K HAPVLAI G VP+A G +Y QE + +FRD Sbjct: 63 SGTIGVCMGTVGPGSLHLLNGLYDAKKSHAPVLAICGQVPSAELGAEYFQEVDNDAVFRD 122 Query: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRERTP 183 VAA+ +TS Q P V QA+ AYA GV+ L +P D IG+ ++H R Sbjct: 123 VAAFRHTVTSASQMPRVLEQAVQTAYATPGVSVLTLPGD-IGSAEVAKDSAVHITRVPAR 181 Query: 184 LSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLVGYD 243 L+P +I A L++ A +V + G G R + VL LA +L AP++ T + K+ + D Sbjct: 182 LTPDDDEITRAVRLLDDAKTVTMLVGAGARESRASVLQLADRLAAPMVLTLKAKEGLEDD 241 Query: 244 HPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGRRAP 303 +P IG GLIG +A +A +LM+G+D+PY ++LP + +QID R +GRR P Sbjct: 242 NPFQIGQSGLIGNPATREAFESAGALLMIGTDFPYPDWLPRSTPTVQIDTRAGHIGRRTP 301 Query: 304 VTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERW--------NAKLDSA------ 349 V +G+ G ++ LL RV K D + L++A + E W + + D + Sbjct: 302 VDVGVVGDAGLSIAALLNRVRSKDDRSHLEKARSSYEDWQGHQRRLTDPEFDQSLVGKVR 361 Query: 350 AEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAA 409 + + KI+P+++A I A +D F DTG+ T+W + RG +R++ SFN + Sbjct: 362 SWLDNTEDKIRPEALATLIDTHAAEDTVFTTDTGMSTVWLARCVTMRGSRRLIGSFNLGS 421 Query: 410 VGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVH 469 + +L A G ALDR+RQVV GDGG TMLLG+ +T+V + LPVK++VF+N G+V Sbjct: 422 MANALPHALGAAALDRQRQVVAFCGDGGLTMLLGDVLTAVAYDLPVKLIVFDNGRLGMVK 481 Query: 470 LEMEEAGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVF 529 LE E+ G+P F G+E NPD AA A A G V PE LE +Q LA+PGP +LDV Sbjct: 482 LEQEQGGLPEF-GTELANPDLAAVATAMGMPAARVTEPEALEAAVQAALASPGPYLLDVV 540 Query: 530 IDPDELPVMPHIKMEQIWRFGLAKVRESLIA 560 +P+E+ + P ++Q W F +AK++E +++ Sbjct: 541 TNPEEIALPPKTSIDQAWGFAIAKMKEGIVS 571 >ref|ZP_06914413.1| pyruvate oxidase [Streptomyces sviceus ATCC 29083] gb|EDY59701.1| pyruvate oxidase [Streptomyces sviceus ATCC 29083] Length = 587 Score = 448 bits (1152), Expect = e-123, Method: Compositional matrix adjust. Identities = 250/580 (43%), Positives = 349/580 (60%), Gaps = 28/580 (4%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA +++D L E+GV Q+FGVVGDALNPLTDAIR + +EW+G RHEE AA AA Q Sbjct: 1 MARTVARVIVDALSELGVHQVFGVVGDALNPLTDAIRTTENLEWVGCRHEEAAAFAASAQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 ++L+G L VC GT GPG+ HL+ GLY+A K H PVLAI+G VP A GTDY QE + L Sbjct: 61 SQLSGTLGVCMGTVGPGSVHLLNGLYDAAKSHTPVLAIAGQVPLAELGTDYFQEVDNDAL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F DVA + ITSPDQ P + A+ A ++GVA L +P D+ + + P+ +L Sbjct: 121 FSDVAVFRATITSPDQLPQMLETAVRHALGRKGVAVLTVPGDLGERELTSDRPARFSL-- 178 Query: 181 RTPLS-PPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKD- 238 PLS P + ++ AA+L++ + V + G G R A ++VLALA +L AP++ T + K+ Sbjct: 179 NAPLSRPEESAVRRAAELLDRSERVTLLVGRGARAAREDVLALADRLAAPMVLTLKAKEG 238 Query: 239 LVGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVL 298 G +P +G GLIG AL A+ +L+LG+D+PY ++ P VIQ+D + Sbjct: 239 FEGDTNPFQVGQTGLIGNPAAASALQDADTLLLLGTDFPYRDWYPEGKTVIQVDTEAAHI 298 Query: 299 GRRAPVTLGITGSVAPAVQQLLERV-------EGKTDGAFLQQANAHRERW---NAKLDS 348 GRR PV +G+ G V+ LL + +G D + L++A E W A+L Sbjct: 299 GRRVPVDVGLVGDTGATVRDLLAHLAAEPPGTDGARDRSHLEKARERFEHWREGQARLAD 358 Query: 349 AAEIK------------RHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQR 396 A K R H I+P+++A + LADDDA F DTG+ T+W ++ R Sbjct: 359 PAHDKGLLGRMRSSLDNRTH-DIRPEALAAVVDRLADDDAVFTSDTGMATVWLSRFVEMR 417 Query: 397 GGQRILASFNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVK 456 G ++++ S+N ++ ++ QA G Q LDRERQVV GDGG +MLLG+ MT H+LPVK Sbjct: 418 GERQLIGSYNLGSMANAMPQALGAQCLDRERQVVAFCGDGGLSMLLGDLMTLKTHQLPVK 477 Query: 457 VVVFNNREWGLVHLEMEEAGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQ 516 +VVF+NR G+V LE E+ G+P F G+ NPDFAA A A G G V P +LE +++ Sbjct: 478 LVVFDNRRLGMVKLEQEQVGLPEF-GTVLDNPDFAAVATAMGIAGIRVTDPADLEKAVRR 536 Query: 517 LLAAPGPAVLDVFIDPDELPVMPHIKMEQIWRFGLAKVRE 556 PGP +LDV +PDE+ V +EQ W F +AKV+E Sbjct: 537 AFDTPGPVLLDVLTNPDEIAVPAKPTVEQGWGFAVAKVKE 576 >ref|YP_003511560.1| thiamine pyrophosphate protein TPP binding domain-containing protein [Stackebrandtia nassauensis DSM 44728] gb|ADD42467.1| thiamine pyrophosphate protein TPP binding domain protein [Stackebrandtia nassauensis DSM 44728] Length = 576 Score = 448 bits (1152), Expect = e-123, Method: Compositional matrix adjust. Identities = 251/574 (43%), Positives = 342/574 (59%), Gaps = 20/574 (3%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TV++ L+D L G+ ++GVVGDALN +TDAIRR I+WI RHEE AA AA Sbjct: 1 MAKTVSETLVDGLVGCGITHVYGVVGDALNSVTDAIRRTDGIDWIATRHEEVAAFAAGAA 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+LTGRL VC GT GPGA HL+ GLY+A K HAPVLAI+G VP G++Y QE + L Sbjct: 61 AQLTGRLGVCAGTVGPGAIHLLNGLYDAAKSHAPVLAITGQVPLPELGSNYFQEVDNDHL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FRDV+ +++ ITSP Q P + QAI A ++RGVA L++P DV + SG P + E Sbjct: 121 FRDVSVFNRTITSPGQVPQLIEQAIEAAVSRRGVAVLSLPGDVGPQQVSGK-PKVRVFNE 179 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 P + AA LIN V I AG G GA D VL LA L AP++ T +GK+ Sbjct: 180 PAASVPSDKLLDQAAKLINGRDRVTILAGAGASGARDAVLGLAGSLAAPIVVTLKGKETF 239 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 +D+P+ +G GLIG AL E++LM+G+D+PY ++ P V+QID +GR Sbjct: 240 DWDNPYQVGQTGLIGNPAAVAALEDCELLLMIGTDFPYRDWYPEGPTVVQIDAVSEHIGR 299 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWN----------------A 344 R V LG+ G A V+ L R+ G L +A +W+ Sbjct: 300 RTRVDLGLVGDAALTVEALAPRLRGNESRRHLDKAVKAFRKWHVGQERLAAPDHDREPVG 359 Query: 345 KLDSAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILAS 404 +L S + HH I+P+SVA A+++LADD A F DTG+ T+W +++ R GQR+L S Sbjct: 360 ELRSLVDNPDHH--IRPESVALAVNELADDTAIFTADTGMSTVWLSRFVQFRQGQRLLGS 417 Query: 405 FNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNRE 464 FN ++ ++ QA G +RQV+ GDGG TMLLG+ +T+V +RLPVK++VF+N Sbjct: 418 FNLGSMANAMPQAIGAATAFPDRQVIAFCGDGGLTMLLGDILTAVRYRLPVKLIVFDNHR 477 Query: 465 WGLVHLEMEEAGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPA 524 G+V LE E+AG+P F G+ PNPD AA A A G +G V P EL GL + AA GP Sbjct: 478 LGMVKLEQEQAGLPEF-GTVLPNPDLAAVAEAMGAKGIRVTEPGELHKGLAEAFAADGPV 536 Query: 525 VLDVFIDPDELPVMPHIKMEQIWRFGLAKVRESL 558 ++DV +P+E+ V + Q W F +AKV+E+L Sbjct: 537 LVDVLTNPEEVSVPGKVLPGQAWGFAIAKVKETL 570 >ref|YP_001990540.1| pyruvate dehydrogenase [Rhodopseudomonas palustris TIE-1] gb|ACF00065.1| thiamine pyrophosphate protein TPP binding domain protein [Rhodopseudomonas palustris TIE-1] Length = 576 Score = 447 bits (1151), Expect = e-123, Method: Compositional matrix adjust. Identities = 243/557 (43%), Positives = 339/557 (60%), Gaps = 4/557 (0%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 MP VAD +++TL++ GVR+IFGVVGD+LN LT+A+R I+W+ VRHEE AA AA + Sbjct: 1 MP-KVADRIVETLHQAGVRRIFGVVGDSLNGLTEALREHGGIQWVHVRHEEVAAFAAAGE 59 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 +++TG LAVC G+ GPG HL+ GLY+A++ PVLAI+ +P+A G Y QE P+ L Sbjct: 60 SQITGELAVCAGSCGPGNLHLINGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNL 119 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVI--GAKTSGSVPSLHTL 178 FR+ + Y +++T P Q V AI A +RGVA + IP DV A + G P L Sbjct: 120 FRECSVYCEMVTDPHQFDYVIENAIRAAIGRRGVAVVVIPGDVALHEASSRGVSPVSGLL 179 Query: 179 RERTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKD 238 ++P +I+A A L+N AG V +F G GC GA DE++ALA L++P++H GK+ Sbjct: 180 PPAPVVTPGEPEIEALATLLNDAGKVTLFCGRGCAGAHDELMALAEALKSPIVHALGGKE 239 Query: 239 LVGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVL 298 V Y +P+ +G G IG + G +A+HA +V++MLG+D+PY +FLP K+ Q+D R L Sbjct: 240 YVEYGNPYDVGMTGFIGFSSGYEAMHACDVLVMLGTDFPYKQFLPGDVKIAQVDLRAEHL 299 Query: 299 GRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKK 358 GRR + LG+ G V ++ LL ++ KTD L AH LD A+ HK Sbjct: 300 GRRCKLDLGVVGGVRETIEALLPKLTTKTDRGHLDHCVAHYVSAREGLDDLAKGTPGHKP 359 Query: 359 IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQAN 418 I PQ +A+ ISD A +DA F D G T+W +++ G +R++ S + ++ +L QA Sbjct: 360 IHPQYLAKLISDSAAEDAVFSFDVGTPTIWAARYLKMNGRRRLIGSLAHGSMANALPQAI 419 Query: 419 GIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMP 478 G+QA RQV+ GDGGFTML+G+ +T + +LPVKVV+FNN G V LEM+ AG Sbjct: 420 GVQAAQPGRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFV 479 Query: 479 SFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVM 538 G++ NPDFAA ARA G G V P +L + + LA GPAVLDV EL + Sbjct: 480 EL-GTDLQNPDFAAMARAVGIHGVRVEDPGDLPAAVAEFLAHDGPAVLDVVTAKQELSMP 538 Query: 539 PHIKMEQIWRFGLAKVR 555 P I EQ+ F L VR Sbjct: 539 PTIGAEQVKGFSLWLVR 555 >ref|YP_483825.1| pyruvate dehydrogenase [Rhodopseudomonas palustris HaA2] gb|ABD04914.1| pyruvate dehydrogenase (cytochrome) [Rhodopseudomonas palustris HaA2] Length = 576 Score = 442 bits (1138), Expect = e-122, Method: Compositional matrix adjust. Identities = 239/555 (43%), Positives = 336/555 (60%), Gaps = 3/555 (0%) Query: 3 ATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQAK 62 + VAD +++TL++ GV +IFGVVGD+LN LT+A+R+ IEW+ VRHEE AA AA +++ Sbjct: 2 SNVADRIVETLHQAGVERIFGVVGDSLNGLTEALRQHGSIEWVHVRHEEVAAFAAAGESQ 61 Query: 63 LTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFR 122 +TG+LAVC G+ GPG HL+ GLY+A++ PVLAI+ +P+A G Y QE P+ LFR Sbjct: 62 ITGKLAVCAGSCGPGNLHLINGLYDAQRTRTPVLAIAAQIPSAEIGGGYFQETHPQNLFR 121 Query: 123 DVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVI--GAKTSGSVPSLHTLRE 180 + + Y ++++ P Q V AI A QRGVA + IP DV A T G P L Sbjct: 122 ECSVYCELVSDPHQFDYVLENAIRAAVGQRGVAVVVIPGDVALREASTRGVTPVAGLLPP 181 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 ++P + A A L+N AG V +F G GC GA ++ALA L++P++H GK+ V Sbjct: 182 TPIVTPAEPQLDALAALLNGAGRVTLFCGRGCAGAHAPLMALAEALKSPIVHALGGKEHV 241 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 YD+P+ +G G IG A G +A+HA +V+LMLG+D+PY +FLPT +++ Q+D R LGR Sbjct: 242 EYDNPYDVGMTGFIGYASGYEAMHACDVLLMLGTDFPYKQFLPTGARIAQVDIRAENLGR 301 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 R + LG+ G V ++ LL ++ KTD L + A LD A+ K I Sbjct: 302 RCKLALGLVGGVHETIEALLPKLTTKTDRGHLDHSVARYVASRQGLDDLAKGTPGRKPIH 361 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQ +A+ ISD A DDA F D G T+W +++ G +R++ S + ++ +L A G+ Sbjct: 362 PQYLAKLISDGAADDAVFSFDVGTPTIWAARYLKMNGSRRLVGSLVHGSMANALPHAIGV 421 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 QA RQV+ GDGGFTML+G+ +T + +LPVKVV+FNN G V LEM+ AG Sbjct: 422 QAAQPSRQVISLSGDGGFTMLMGDLITLTQMKLPVKVVIFNNGVLGFVALEMKAAGFVEL 481 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 G++ NPDFAA ARA G G V P +L + +LA GPAVLDV EL + P Sbjct: 482 -GTDLQNPDFAAMARAMGIHGVRVEDPGDLPAAVADVLAHDGPAVLDVVTATQELSMPPT 540 Query: 541 IKMEQIWRFGLAKVR 555 I EQ+ F L +R Sbjct: 541 IGAEQVKGFSLWLLR 555 >ref|NP_822130.1| pyruvate dehydrogenase/oxidase FAD and thiamine PPi cofactors [Streptomyces avermitilis MA-4680] dbj|BAC68665.1| putative pyruvate dehydrogenase/oxidase FAD and thiamine PPi cofactors [Streptomyces avermitilis MA-4680] Length = 587 Score = 442 bits (1138), Expect = e-122, Method: Compositional matrix adjust. Identities = 247/581 (42%), Positives = 348/581 (59%), Gaps = 30/581 (5%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA +++D L E+GVRQ+FGVVGDALNP+TDAIR + +EW+G RHEE AA AA Q Sbjct: 1 MARTVARVIVDALSELGVRQVFGVVGDALNPVTDAIRTTEDVEWVGCRHEEAAAFAASAQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 ++L+G L VC GT GPG+ HL+ GLY+A K PVLAI+G VP A G+DY QE + L Sbjct: 61 SQLSGTLGVCMGTVGPGSVHLLNGLYDAAKSRTPVLAIAGQVPLAELGSDYFQEVDNDAL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F DVA + ITSPDQ P + A+ A ++GVA L +P D+ + S P+ +L Sbjct: 121 FSDVAVFRATITSPDQLPQLLETAVRNALGRKGVAVLTVPGDLGDRELSADRPTRFSL-- 178 Query: 181 RTPLS-PPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKD- 238 TP+S P + ++ AA+L++ A + + G G R A +VL LA +L AP++ T + K+ Sbjct: 179 STPVSRPEESAVRRAAELLDRAERITLLVGRGARAARADVLTLADRLAAPMVLTLKAKEG 238 Query: 239 LVGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVL 298 G +P +G GLIG AL A+ +L+LG+D+PY ++ P VIQ+D + Sbjct: 239 FEGEANPFQVGQTGLIGNPAAASALQDADTLLLLGTDFPYRDWYPDGRTVIQVDSEAAHI 298 Query: 299 GRRAPVTLGITGSVAPAVQQLLERV-------EGKTDGAFLQQANAHRERWNA------- 344 GRR PV +G+ G V+ LL + EG D + L+ A +W A Sbjct: 299 GRRVPVEVGLVGDTGATVRDLLGHLATAPPGTEGARDRSHLEDARKSFGKWRASQARLAD 358 Query: 345 ---------KLDSAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQ 395 ++ SA + + H I+P+++A + +AD++A F DTG+ T+W ++ Sbjct: 359 PAHDKGLVGRVRSALDNRAH--DIRPEALAAVVDRVADENAVFTSDTGMATVWLSRFVEM 416 Query: 396 RGGQRILASFNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPV 455 RG +R+L S+N ++ ++ QA G Q LDR RQVV GDGG +MLLG+ MT RLPV Sbjct: 417 RGDRRLLGSYNLGSMANAMPQALGAQCLDRGRQVVAFCGDGGLSMLLGDLMTLKTSRLPV 476 Query: 456 KVVVFNNREWGLVHLEMEEAGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQ 515 K+VVF+NR G+V LE E+AG+P F G+ NPDFAA A A G G V P ELE ++ Sbjct: 477 KLVVFDNRRLGMVKLEQEQAGLPEF-GTVLDNPDFAAVATAMGITGIRVTDPAELEESVR 535 Query: 516 QLLAAPGPAVLDVFIDPDELPVMPHIKMEQIWRFGLAKVRE 556 + A PGP +LDV +PDE+ V +EQ W F +AKV+E Sbjct: 536 RAFATPGPVLLDVLTNPDEIAVPAKPTVEQGWGFAVAKVKE 576 >ref|YP_004859336.1| thiamine pyrophosphate TPP-binding domain-containing protein [Bacillus coagulans 36D1] gb|AEP00556.1| thiamine pyrophosphate TPP-binding domain-containing protein [Bacillus coagulans 36D1] Length = 574 Score = 442 bits (1137), Expect = e-122, Method: Compositional matrix adjust. Identities = 228/558 (40%), Positives = 340/558 (60%), Gaps = 1/558 (0%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M T+AD+LID+L GV++I+G+VGD+LN + D+IRR RIEW+GVRHEE AA AA + Sbjct: 1 MAKTIADLLIDSLLNAGVKRIYGIVGDSLNAVLDSIRRSGRIEWVGVRHEEVAAFAAGAE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A L +AVC G++GPG HL+ GLY+ + PVLAI+ +P++ G++Y Q+ PEL+ Sbjct: 61 ATLNNSIAVCAGSSGPGNLHLINGLYDCNRSRVPVLAIAAHIPSSEIGSEYFQQTRPELI 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F+D A + + + P+Q P + + A+ QA A +GV+ L +P DV G + +VP + Sbjct: 121 FKDCAHFIETVQRPEQMPLMVNMAMQQAVANQGVSVLVLPGDVAGLDDNVAVPQVPVHVA 180 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 + + P D+ A +N V + G GC+GA DE++ L +KL++P++ RGK+ + Sbjct: 181 KPVVKPSEEDLTQLASYLNDGKKVTLLCGAGCQGAHDEIMELCNKLKSPMVVALRGKEFL 240 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 YD+P++ G GLIG + G DA+ + +LMLG+D+PY +F P +KV+Q+D R LGR Sbjct: 241 EYDNPYFAGLTGLIGYSSGYDAMMECDTLLMLGTDFPYRQFYPKNAKVLQVDIRSEHLGR 300 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 R P+TLG+ G V V+ LL ++ K + L++ ++ LD A K K I Sbjct: 301 RTPLTLGVVGDVKDTVEALLPMIQEKDNSKHLEKHVKAYQKVREDLDELAVGKPGRKPIH 360 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQ + + +SDLAD+DA F D G TLW +++ G +R+L SFN+ + +L QA G Sbjct: 361 PQYLTKLVSDLADEDAIFTCDVGTPTLWAARYVQMNGKRRLLGSFNHGTMANALPQAIGA 420 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 +QVV GDGG +ML+G+ +T +H+LPVKVVVFNN V LEM+ AG P Sbjct: 421 AVAQPGKQVVSLSGDGGLSMLMGDMLTLRQHKLPVKVVVFNNTALSFVELEMKAAGYPE- 479 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 G+ +P++AA A A G +G V P +LEG L++ GPAV+DV ++ EL + P Sbjct: 480 TGTGLDSPNYAAIAEAMGMKGIRVEDPADLEGALRESFEHDGPAVIDVAVNRQELSLPPK 539 Query: 541 IKMEQIWRFGLAKVRESL 558 I EQ F L ++ L Sbjct: 540 ITYEQAHGFTLWMLKAVL 557