BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0121.p (193 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777806.1| small-conductance mechanosensitive channel p... 385 e-105 ref|YP_001899317.1| small-conductance mechanosensitive channel-l... 258 4e-67 ref|YP_001566265.1| small-conductance mechanosensitive channel-l... 192 2e-47 ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicyclip... 191 4e-47 ref|YP_004486751.1| mechanosensitive ion channel MscS [Delftia s... 191 5e-47 ref|YP_987451.1| small-conductance mechanosensitive channel-like... 180 1e-43 ref|YP_002554040.1| small-conductance mechanosensitive channel-l... 179 2e-43 ref|YP_004146641.1| mechanosensitive ion channel MscS [Pseudoxan... 176 1e-42 ref|YP_003277034.1| hypothetical protein CtCNB1_0992 [Comamonas ... 175 4e-42 ref|ZP_03544782.1| small-conductance mechanosensitive channel-li... 174 6e-42 >ref|YP_003777806.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ65898.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 193 Score = 385 bits (989), Expect = e-105, Method: Compositional matrix adjust. Identities = 192/193 (99%), Positives = 193/193 (100%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA Sbjct: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI Sbjct: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR 180 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR Sbjct: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR 180 Query: 181 IVRRWSDGSGALF 193 IVRRWSDGSG+LF Sbjct: 181 IVRRWSDGSGSLF 193 >ref|YP_001899317.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] gb|ACD26885.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] Length = 187 Score = 258 bits (658), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 125/187 (66%), Positives = 146/187 (78%), Gaps = 1/187 (0%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 M+K LQ+IH+ LPQWLADWFD+LVP EV+LI GA +R+AR L+ +L TY P K Sbjct: 1 MNKLLQAIHESLPQWLADWFDILVPAIEVVLIAAGAWLAVRIARRLLRKLTNTYSLPGKV 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AAL+ RV+ VY A LWALERMGVS LW+AFTGFA VGAVAFFAAWSVLSNLFCA+ Sbjct: 61 AALSLRVVATIVYVAATLWALERMGVSGTVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH-EGGETTLQLPNSLFFQ 179 LIY T FR+GDV+E+LE G+KPG+KGRVLD+NLVYTTL E EG E LQLPNSL FQ Sbjct: 121 LIYVTGAFRIGDVVELLENGEKPGIKGRVLDVNLVYTTLLETTVEGQEVVLQLPNSLVFQ 180 Query: 180 RIVRRWS 186 R++RRW Sbjct: 181 RVLRRWK 187 >ref|YP_001566265.1| small-conductance mechanosensitive channel-like protein [Delftia acidovorans SPH-1] gb|ABX37880.1| small-conductance mechanosensitive channel-like protein [Delftia acidovorans SPH-1] Length = 226 Score = 192 bits (488), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 95/192 (49%), Positives = 127/192 (66%), Gaps = 2/192 (1%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 M + ++ LP W+ DW + ++P A+++LI+ A + R+ R LI R YG P + Sbjct: 1 MFEVMRRFQAHLPTWMQDWLEFIIPGAQILLIIGAAWLLNRLVRRLIARAGRHYGLPPEL 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 + V + + + LER+GVS+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+ Sbjct: 61 LVPLRGVFRWILIAATVPLVLERLGVSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFF 178 LI PFR+GD IEVL+ +KPG KGRV+DIN++YTTL + H +G LQ+PNSL F Sbjct: 121 LIVIVGPFRLGDYIEVLDTAEKPGAKGRVIDINMLYTTLQDAHAPDGSPNLLQIPNSLIF 180 Query: 179 QRIVRRWSDGSG 190 QR+VRRW G G Sbjct: 181 QRVVRRWKGGLG 192 >ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicycliphilus denitrificans BC] ref|YP_004387206.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] gb|ADV00883.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans BC] gb|AEB83690.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] Length = 220 Score = 191 bits (486), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 109/190 (57%), Positives = 132/190 (69%), Gaps = 3/190 (1%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MS L+ + LP W DW DV+VP ++ LIVL AL + + R LI R + Y FP++ Sbjct: 1 MSPMLERLKTILPPWAHDWLDVIVPGMQIALIVLAALLLHYIVRRLIRRASDHYQFPHEL 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 V+ + + GGA L ALER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA Sbjct: 61 LTPVNAVVRWLILGGAALLALERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAF 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFF 178 LI T PFRVGD IE+L+ +KPG GRVLDINL+YTTL A E G T LQ+PN+L F Sbjct: 121 LILTVGPFRVGDHIELLDTAEKPGALGRVLDINLLYTTLEDATAPEPG-TLLQIPNALIF 179 Query: 179 QRIVRRWSDG 188 QR+VRRW G Sbjct: 180 QRVVRRWRAG 189 >ref|YP_004486751.1| mechanosensitive ion channel MscS [Delftia sp. Cs1-4] gb|AEF88396.1| MscS Mechanosensitive ion channel [Delftia sp. Cs1-4] Length = 222 Score = 191 bits (485), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 94/181 (51%), Positives = 123/181 (67%), Gaps = 2/181 (1%) Query: 12 LPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALTQRVLGFF 71 LP W+ DW + ++P A+++LI+ A + R+ R LI R YG P + + V + Sbjct: 8 LPTWMQDWLEFIIPGAQILLIIGAAWLLNRLVRRLIARAGRHYGLPPELLVPLRGVFRWI 67 Query: 72 VYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYTTQPFRVG 131 + + LER+GVS+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI PFR+G Sbjct: 68 LIAATVPLVLERLGVSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVIVGPFRLG 127 Query: 132 DVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIVRRWSDGS 189 D IEVL+ +KPG KGRV+DIN++YTTL + H +G LQ+PNSL FQR+VRRW G Sbjct: 128 DYIEVLDTAEKPGAKGRVIDINMLYTTLQDAHAPDGSPNLLQIPNSLIFQRVVRRWKGGL 187 Query: 190 G 190 G Sbjct: 188 G 188 >ref|YP_987451.1| small-conductance mechanosensitive channel-like protein [Acidovorax sp. JS42] gb|ABM43375.1| Small-conductance mechanosensitive channel-like protein [Acidovorax sp. JS42] Length = 222 Score = 180 bits (456), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 102/188 (54%), Positives = 130/188 (69%), Gaps = 3/188 (1%) Query: 4 FLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAAL 63 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 1 MLPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLTP 60 Query: 64 TQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIY 123 V+ + + G A L LER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA LI+ Sbjct: 61 INAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIF 120 Query: 124 TTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFFQRI 181 T PFRVGD IE+L+ DKPG GRV+DINL+Y TL A E G T LQ+PN+L FQR+ Sbjct: 121 TVGPFRVGDHIELLDTADKPGALGRVMDINLLYITLEDATAPEPG-TLLQIPNTLIFQRV 179 Query: 182 VRRWSDGS 189 VRRW G+ Sbjct: 180 VRRWRAGT 187 >ref|YP_002554040.1| small-conductance mechanosensitive channel-like protein [Acidovorax ebreus TPSY] gb|ACM34040.1| small-conductance mechanosensitive channel-like protein [Acidovorax ebreus TPSY] Length = 222 Score = 179 bits (455), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 102/188 (54%), Positives = 130/188 (69%), Gaps = 3/188 (1%) Query: 4 FLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAAL 63 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 1 MLPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLIP 60 Query: 64 TQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIY 123 V+ + + G A L LER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA LI+ Sbjct: 61 INAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIF 120 Query: 124 TTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFFQRI 181 T PFRVGD IE+L+ DKPG GRV+DINL+Y TL A E G T LQ+PN+L FQR+ Sbjct: 121 TVGPFRVGDHIELLDTADKPGALGRVMDINLLYITLEDATAPEPG-TLLQIPNTLIFQRV 179 Query: 182 VRRWSDGS 189 VRRW G+ Sbjct: 180 VRRWRAGT 187 >ref|YP_004146641.1| mechanosensitive ion channel MscS [Pseudoxanthomonas suwonensis 11-1] gb|ADV27410.1| MscS Mechanosensitive ion channel [Pseudoxanthomonas suwonensis 11-1] Length = 204 Score = 176 bits (446), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 102/183 (55%), Positives = 129/183 (70%), Gaps = 7/183 (3%) Query: 8 IHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALTQRV 67 + LP+WL W +VP + +LIVL A + ++ R L+ RL G P + + RV Sbjct: 1 MKHHLPEWLQGWLGTIVPIGQALLIVLAAWLLQQLVRRLVRRLMDRRGLPPEMGVVLHRV 60 Query: 68 LGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYTTQP 127 + GALL LER+GVS LW+AFT FAAVGAVAFFAAWSVLSN+FC +LI+TT+P Sbjct: 61 SAALIGFGALLMVLERLGVSGTVLWTAFTSFAAVGAVAFFAAWSVLSNIFCTVLIFTTRP 120 Query: 128 FRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAE-----EHEGGETTLQLPNSLFFQRIV 182 FR+ D IEV+E G+KPGLKGRV+DINL+YTTL E EH G ++LQ+PNSLFFQR+V Sbjct: 121 FRLYDYIEVVENGEKPGLKGRVVDINLIYTTLQEVPRDPEHAG--SSLQVPNSLFFQRVV 178 Query: 183 RRW 185 RRW Sbjct: 179 RRW 181 >ref|YP_003277034.1| hypothetical protein CtCNB1_0992 [Comamonas testosteroni CNB-2] ref|ZP_07044085.1| hypothetical protein CTS44_07790 [Comamonas testosteroni S44] gb|ACY31738.1| conserved hypothetical protein [Comamonas testosteroni CNB-2] gb|EFI62128.1| hypothetical protein CTS44_07790 [Comamonas testosteroni S44] Length = 218 Score = 175 bits (443), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 89/186 (47%), Positives = 125/186 (67%), Gaps = 2/186 (1%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 ++ + LP W+ DW ++L+P ++ LI+L A I + + +I R Y P++ Sbjct: 1 MRRLQMHLPDWMQDWLEILIPGVQIFLIILVAWLINWLIKRIISRAGKAYDLPHELLMPL 60 Query: 65 QRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYT 124 + V + + A+L LER+G+S+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI T Sbjct: 61 RGVFRWIIIAAAILAILERLGMSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVT 120 Query: 125 TQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIV 182 PFR+GD IEVL+ +KPG KGRV+D+N++Y TL ++ G LQ+PNSL FQR++ Sbjct: 121 VGPFRLGDYIEVLDTAEKPGAKGRVIDLNMLYITLQDDSAPAGMPNLLQIPNSLIFQRVI 180 Query: 183 RRWSDG 188 RRW G Sbjct: 181 RRWKGG 186 >ref|ZP_03544782.1| small-conductance mechanosensitive channel-like protein [Comamonas testosteroni KF-1] gb|EED69068.1| small-conductance mechanosensitive channel-like protein [Comamonas testosteroni KF-1] Length = 218 Score = 174 bits (441), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 89/186 (47%), Positives = 125/186 (67%), Gaps = 2/186 (1%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 ++ + LP W+ DW ++L+P ++ LI+L A I + + +I R Y P++ Sbjct: 1 MRRLQMHLPDWMQDWLEILIPGIQIFLIILVAWLINWLIKRIISRAGKAYDLPHELLMPL 60 Query: 65 QRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYT 124 + V + + A+L LER+G+S+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI T Sbjct: 61 RGVFRWIIIAAAVLAILERLGMSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVT 120 Query: 125 TQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIV 182 PFR+GD IEVL+ +KPG KGRV+D+N++Y TL ++ G LQ+PNSL FQR++ Sbjct: 121 VGPFRLGDYIEVLDTAEKPGAKGRVIDLNMLYITLQDDSAPAGMPNLLQIPNSLIFQRVI 180 Query: 183 RRWSDG 188 RRW G Sbjct: 181 RRWKGG 186