BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0112.p (261 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_581805.1| hydrolase or acyltransferase (alpha/beta hydrol... 340 9e-92 ref|ZP_02885012.1| alpha/beta hydrolase fold [Burkholderia grami... 325 3e-87 ref|YP_621110.1| alpha/beta hydrolase fold [Burkholderia cenocep... 309 2e-82 ref|YP_002947895.1| alpha/beta hydrolase fold protein [Variovora... 275 3e-72 ref|YP_001632811.1| 2-hydroxymuconic semialdehyde hydrolase [Bor... 259 4e-67 ref|YP_004614351.1| alpha/beta hydrolase fold protein [Mesorhizo... 211 7e-53 ref|YP_004330470.1| alpha/beta hydrolase fold protein [Pseudonoc... 163 2e-38 ref|YP_145578.1| putative B-ketoadipate enol-lactone hydrolase [... 163 3e-38 gb|EGN93887.1| hypothetical protein SERLA73DRAFT_156032 [Serpula... 150 1e-34 ref|XP_003047693.1| hypothetical protein NECHADRAFT_50720 [Nectr... 150 3e-34 >ref|YP_581805.1| hydrolase or acyltransferase (alpha/beta hydrolase superfamily) [Cupriavidus metallidurans CH34] gb|ABF12855.1| hydrolase or acyltransferase (alpha/beta hydrolase superfamily) [Cupriavidus metallidurans CH34] Length = 260 Score = 340 bits (873), Expect = 9e-92, Method: Compositional matrix adjust. Identities = 165/256 (64%), Positives = 198/256 (77%), Gaps = 2/256 (0%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 MAT I+ +AVE+ GDG P+LCIHGLGGSSNTWTP+LPA+ G KVIR+DLPGS RS L Sbjct: 1 MATEVINGLAVEIYGDGQ--PLLCIHGLGGSSNTWTPVLPAMEGFKVIRIDLPGSARSAL 58 Query: 61 PAQALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVA 120 P ++L+I YV + L AL + V + AHS+GTI+AQHFAV +P+ V SL LFGPL+A Sbjct: 59 PVESLSIAGYVAAIATTLDALAAQQVHVAAHSLGTIIAQHFAVTFPERVRSLALFGPLLA 118 Query: 121 PPEPARPAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPE 180 PPE RPAI ARA+LAR+G +Q+IADAIV GATS+ETK P L LVRESVMRQPPE Sbjct: 119 PPEQGRPAIHARAQLAREGISGMQQIADAIVNGATSRETKEQQPAVLALVRESVMRQPPE 178 Query: 181 GYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGH 240 GYAQSCEALA+A+ A +E+I+VPTLLVTGDQDGVAP A V +M +RI G+ V VL GCGH Sbjct: 179 GYAQSCEALAAAQPARVEQISVPTLLVTGDQDGVAPPAAVQSMGERIAGANVFVLTGCGH 238 Query: 241 WTTFEKTDACIKAIKE 256 WTTFEK C ++E Sbjct: 239 WTTFEKPMECSNLLRE 254 >ref|ZP_02885012.1| alpha/beta hydrolase fold [Burkholderia graminis C4D1M] gb|EDT09444.1| alpha/beta hydrolase fold [Burkholderia graminis C4D1M] Length = 261 Score = 325 bits (834), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 158/247 (63%), Positives = 190/247 (76%), Gaps = 3/247 (1%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 MAT + + +E +GDG APV+CIHGLGGSSN WTP+L A G++VIR+DLPGS RS+L Sbjct: 1 MATTLVQGLYIETQGDG--APVVCIHGLGGSSNNWTPVLGAFEGKRVIRIDLPGSARSEL 58 Query: 61 PAQALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVA 120 PAQ L+I+ YV + L+ +KIE D++AHSMGTIVAQH A+ +P V SL LFGPL+A Sbjct: 59 PAQKLSIDGYVDVIAAALREMKIEQADVVAHSMGTIVAQHLAIRHPQLVKSLALFGPLLA 118 Query: 121 PPEPARPAIRARAELAR-QGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPP 179 PP+ R +R RA LAR +G +QEIADAIV GATS ETKA P+ L LVRESVMRQ P Sbjct: 119 PPDAGREGMRQRAALARDKGVAGLQEIADAIVKGATSDETKAQRPVTLALVRESVMRQSP 178 Query: 180 EGYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCG 239 EGYAQSCEALA A++AP+E+I VPTLLVTGDQDGV V AM +RI G+KV+VL GCG Sbjct: 179 EGYAQSCEALAGAQAAPVEQIKVPTLLVTGDQDGVGKPEAVQAMGERIAGAKVVVLSGCG 238 Query: 240 HWTTFEK 246 HWTTFEK Sbjct: 239 HWTTFEK 245 >ref|YP_621110.1| alpha/beta hydrolase fold [Burkholderia cenocepacia AU 1054] ref|YP_840225.1| alpha/beta hydrolase fold [Burkholderia cenocepacia HI2424] gb|ABF76137.1| alpha/beta hydrolase fold protein [Burkholderia cenocepacia AU 1054] gb|ABK13332.1| alpha/beta hydrolase fold protein [Burkholderia cenocepacia HI2424] Length = 261 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 159/262 (60%), Positives = 187/262 (71%), Gaps = 3/262 (1%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 M T I D+AVE+ G+G PV+CIHGLGGSSN WTP+L A G +V+RLDLPGS RS L Sbjct: 1 MTTTLIRDIAVEITGEGQ--PVVCIHGLGGSSNNWTPVLEAFGGMRVVRLDLPGSARSAL 58 Query: 61 PAQALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVA 120 + L+IE YV V VL L IE I+AHSMGTIVAQH AV +P V SL LFGPL+A Sbjct: 59 ANEPLSIELYVSRVADVLSELGIEQAHIVAHSMGTIVAQHLAVQHPHLVRSLALFGPLLA 118 Query: 121 PPEPARPAIRARAELAR-QGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPP 179 PP+ RPAIRARA L R +G +QEIADAIV GATS ETK+ P+ + LVRESVMRQ P Sbjct: 119 PPDQGRPAIRARAALCRDRGIAGLQEIADAIVKGATSVETKSVRPVTVALVRESVMRQSP 178 Query: 180 EGYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCG 239 EGYAQSC AL+ A A I++I+VPTLLVTGD+D V A V M++RI GS + VL GCG Sbjct: 179 EGYAQSCLALSEASEAAIDQISVPTLLVTGDEDAVGQPALVKNMSERIDGSTLTVLRGCG 238 Query: 240 HWTTFEKTDACIKAIKEALSNN 261 HWTTFEK CI +K N+ Sbjct: 239 HWTTFEKPAECIAELKAFYDND 260 >ref|YP_002947895.1| alpha/beta hydrolase fold protein [Variovorax paradoxus S110] gb|ACS22629.1| alpha/beta hydrolase fold protein [Variovorax paradoxus S110] Length = 262 Score = 275 bits (704), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 154/257 (59%), Positives = 188/257 (73%), Gaps = 4/257 (1%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 M T ++D+ VE+ DGDG +LCIHGLGGSSN WTP+LPAL G + IR DLPGS RS L Sbjct: 1 MTTQVLNDLTVEI--DGDGTALLCIHGLGGSSNNWTPVLPALGGFRRIRPDLPGSARSPL 58 Query: 61 PA--QALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPL 118 PA +AL+IE YV + ++L L + SV ++AHSMGTIVAQH A+ P V SL LFGPL Sbjct: 59 PADGKALSIEHYVDAMLRLLSTLDVGSVHVMAHSMGTIVAQHLALRSPGMVKSLALFGPL 118 Query: 119 VAPPEPARPAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQP 178 APP+ R I ARA AR G +Q+IADAIV ATS +TK P L LVRESVMRQ Sbjct: 119 AAPPDATRSNIAARANAARGGVAAMQDIADAIVKAATSTQTKEQQPAVLALVRESVMRQS 178 Query: 179 PEGYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGC 238 PEGYAQSC ALA A++A +E I VPTLLVTGDQD VAP ANV+AM +RI GS+++VL+ C Sbjct: 179 PEGYAQSCAALADAEAAALEHIDVPTLLVTGDQDVVAPVANVHAMQERIVGSRLVVLDEC 238 Query: 239 GHWTTFEKTDACIKAIK 255 GHWTTFE+ C++ ++ Sbjct: 239 GHWTTFEQPQRCVQELE 255 >ref|YP_001632811.1| 2-hydroxymuconic semialdehyde hydrolase [Bordetella petrii DSM 12804] emb|CAP44543.1| 2-hydroxymuconic semialdehyde hydrolase [Bordetella petrii] Length = 259 Score = 259 bits (661), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 144/256 (56%), Positives = 179/256 (69%), Gaps = 1/256 (0%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 M++ I+ + VE G G G VLCIHGLGG+SN W+PIL A R+VIR DL G+GRS Sbjct: 1 MSSARINGLWVEDSG-GSGEAVLCIHGLGGTSNFWSPILSAFGERRVIRPDLAGAGRSPE 59 Query: 61 PAQALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVA 120 L+I +V ++ VL AL IE ++AHSMGTI+AQH AVA+P V SL LFGPL A Sbjct: 60 APDGLSIAHHVESLVGVLDALGIEQAHVVAHSMGTIIAQHLAVAHPGRVRSLALFGPLAA 119 Query: 121 PPEPARPAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPE 180 P + AR R RAELAR G +QEIADAIV ATS ETKA P+AL LVRESVMRQPPE Sbjct: 120 PADAAREPTRKRAELARSGPAALQEIADAIVKAATSAETKAERPVALALVRESVMRQPPE 179 Query: 181 GYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGH 240 GYA++CEALA A++A IE + +P LLVTGD+DGV A A+A R+ ++ VL+GCGH Sbjct: 180 GYARNCEALAEAQAAAIEPLKIPVLLVTGDEDGVGTPAGTAALAGRLTDARSYVLQGCGH 239 Query: 241 WTTFEKTDACIKAIKE 256 WTT+EK I+ + + Sbjct: 240 WTTYEKPFESIRILTD 255 >ref|YP_004614351.1| alpha/beta hydrolase fold protein [Mesorhizobium opportunistum WSM2075] gb|AEH90257.1| alpha/beta hydrolase fold protein [Mesorhizobium opportunistum WSM2075] Length = 259 Score = 211 bits (538), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 115/246 (46%), Positives = 148/246 (60%), Gaps = 4/246 (1%) Query: 1 MATLFIDDVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAGRKVIRLDLPGSGRSKL 60 M T+ + V EV G+G V+ IHGLGG+SN + P + AL+G +VIRLDLPGSGRS Sbjct: 1 MTTVTVGQVVAEVVGEGTA--VVMIHGLGGTSNMFQPQMAALSGYRVIRLDLPGSGRSPR 58 Query: 61 PAQALTIEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVA 120 P + LTIE V + + + + S + HSMGTIV Q A A P V SL LFG L Sbjct: 59 PIEPLTIEGMSEAVIRAMAGMGVTSAHFVGHSMGTIVCQQIAAAQPSLVASLALFGALAE 118 Query: 121 PPEPARPAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPE 180 P E R + RA LAR G + ++AD IVA A S T+ ++P A+ VRES+ RQ PE Sbjct: 119 PAEATRQGLANRARLARSGG--IADVADQIVANAISAHTRETSPAAVAFVRESITRQDPE 176 Query: 181 GYAQSCEALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGH 240 YA++CEALA A + I+ PTLLVTGD D V P A+A +I+G+ L+ CGH Sbjct: 177 SYARTCEALAKATAVDARRISAPTLLVTGDADTVNPPGVGQALADKIKGAIFSSLDRCGH 236 Query: 241 WTTFEK 246 W T E Sbjct: 237 WATVES 242 >ref|YP_004330470.1| alpha/beta hydrolase fold protein [Pseudonocardia dioxanivorans CB1190] gb|AEA22617.1| alpha/beta hydrolase fold protein [Pseudonocardia dioxanivorans CB1190] Length = 253 Score = 163 bits (413), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 100/239 (41%), Positives = 140/239 (58%), Gaps = 6/239 (2%) Query: 8 DVAVEVEGDGDGAPVLCIHGLGGSSNTWTPILPALAG-RKVIRLDLPGSGRSKLPAQALT 66 ++AVEVEG G VL +HGLGG+SN + ALA V+R+D G+GRS L A+ ++ Sbjct: 7 ELAVEVEGSG--PAVLLVHGLGGTSNFYQVQADALAATHTVVRVDSAGAGRSDL-AEGIS 63 Query: 67 IEAYVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVAPPEPAR 126 IE++ + V+ L IES ++ HSMGT+V + FA +P+ V SL L G + P + R Sbjct: 64 IESHAEDLLGVMDELGIESAAVVGHSMGTLVVRTFAARHPERVRSLALLGTVREPADAGR 123 Query: 127 PAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSC 186 A R RA + R A+ +A +VA S+ T+ +P VRE VMRQ PEGYA++C Sbjct: 124 QAQRDRAAVLR--ADGTAAVAPGVVANGLSETTRTQHPEIAAFVRELVMRQDPEGYARNC 181 Query: 187 EALASAKSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFE 245 EALA+A + ++P LL+TGD+D V P +A + V VL G GHWT E Sbjct: 182 EALAAATDPGPVDPSLPLLLITGDEDKVGPPDASRELADAHGSATVDVLPGVGHWTALE 240 >ref|YP_145578.1| putative B-ketoadipate enol-lactone hydrolase [Ralstonia metallidurans CH34] emb|CAI11230.1| putative B-ketoadipate enol-lactone hydrolase [Cupriavidus metallidurans CH34] Length = 120 Score = 163 bits (412), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 78/114 (68%), Positives = 91/114 (79%) Query: 143 VQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCEALASAKSAPIEEIAV 202 +Q+IADAIV GATS+ETK P L LVRESVMRQPPEGYAQSCEALA+A+ A +E+I+V Sbjct: 1 MQQIADAIVNGATSRETKEQQPAVLALVRESVMRQPPEGYAQSCEALAAAQPARVEQISV 60 Query: 203 PTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFEKTDACIKAIKE 256 PTLLVTGDQDGVAP A V +M +RI G+ V VL GCGHWTTFEK C ++E Sbjct: 61 PTLLVTGDQDGVAPPAAVQSMGERIAGANVFVLTGCGHWTTFEKPMECSNLLRE 114 >gb|EGN93887.1| hypothetical protein SERLA73DRAFT_156032 [Serpula lacrymans var. lacrymans S7.3] gb|EGO19252.1| hypothetical protein SERLADRAFT_411758 [Serpula lacrymans var. lacrymans S7.9] Length = 285 Score = 150 bits (380), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 84/237 (35%), Positives = 130/237 (54%), Gaps = 5/237 (2%) Query: 21 PVLCIHGLGGSSNTWTPILPALA---GRKVIRLDLPGSGRSKLPAQALTIEAYVLTVRQV 77 P++ +HGLGGSS + P++ R+VI DL G G S L ++IE + ++ + Sbjct: 47 PIVFVHGLGGSSTNYGPVIQTSGLTREREVITFDLEGHGLSPLSGGNISIEGFADSIALI 106 Query: 78 LKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVAPPEPARPAIRARAELAR 137 L++ KI+ I+ HSMG I+ A YP V L+L GP+ A PE A+ ARA R Sbjct: 107 LQSAKIDKAVIVGHSMGGIIVTTLAAKYPAIVDKLILIGPVKAFPEAGVKALTARAASVR 166 Query: 138 QGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCEALASAKSAPI 197 + + + IAD +VA TS+ T++S P+A VR S++ P E YA +C AL A Sbjct: 167 E--QGMSGIADTVVAAGTSQITQSSRPLAKYAVRASLLTTPAEAYALACLALTKAADPDY 224 Query: 198 EEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFEKTDACIKAI 254 I +P L++ G +D P A V +++++ +KV+ E GHW FE+ + + I Sbjct: 225 SLITMPVLILAGTEDKTCPEATVIFLSEKMPQAKVVTFENVGHWHLFEEVEGVSREI 281 >ref|XP_003047693.1| hypothetical protein NECHADRAFT_50720 [Nectria haematococca mpVI 77-13-4] gb|EEU41980.1| hypothetical protein NECHADRAFT_50720 [Nectria haematococca mpVI 77-13-4] Length = 590 Score = 150 bits (378), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 92/246 (37%), Positives = 139/246 (56%), Gaps = 11/246 (4%) Query: 22 VLCIHGLGGSSNTWTPILP--ALAGRKVIRL-DLPGSGRSKL-PAQALTIEAYVLTVRQV 77 ++ +HGLGG+ + WTP++ +LA + L DL G G S P L++E++ ++ + Sbjct: 346 IVFVHGLGGTLDYWTPLISRLSLAEENTLHLFDLEGHGLSTTHPLSTLSLESFASDIKSI 405 Query: 78 LKALKIES---VDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVAP-PEPARPAIRARA 133 A + + + AHS+G + + F + P V+ LVL GP +P PE A ARA Sbjct: 406 FDAAGVTTSAPATLFAHSLGCLASLKFTIDNPGLVSKLVLVGPPPSPLPEAASKGAYARA 465 Query: 134 ELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCEALASA- 192 L R ++ ++ + DA+V S +K +NP A T VR S++ Q PE YA++ ALA A Sbjct: 466 ALVR--SKGIRAVVDAVVDAGISSHSKKANPTASTAVRLSLLGQDPESYAKATWALAGAT 523 Query: 193 KSAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFEKTDACIK 252 + +E I TL+VTGD+D V+P A V A RI+GSK +VL+ GHW FE D + Sbjct: 524 QKLNVEAIEAKTLIVTGDEDRVSPPALVEEYAGRIKGSKAVVLKNVGHWHVFEDVDGVAE 583 Query: 253 AIKEAL 258 A+ E L Sbjct: 584 AVGEFL 589