BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0419.p (222 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774554.1| HAD superfamily haloalkanoic acid dehalogena... 467 e-130 ref|YP_004750979.1| Haloacid dehalogenase, type II [Collimonas f... 434 e-120 ref|YP_002541869.1| haloacid dehalogenase, type II [Agrobacteriu... 332 2e-89 ref|YP_002822840.1| hydrolase [Sinorhizobium fredii NGR234] >gi|... 330 1e-88 ref|YP_546921.1| haloacid dehalogenase [Polaromonas sp. JS666] >... 330 1e-88 ref|ZP_00946701.1| Probable 2-haloacid halidohydrolase IVa [Rals... 326 1e-87 ref|YP_004551835.1| haloacid dehalogenase type II [Sinorhizobium... 325 2e-87 ref|YP_004685539.1| haloacid dehalogenase type II [Cupriavidus n... 325 2e-87 emb|CCA82055.1| 2-haloacid halidohydrolase type II [blood diseas... 325 3e-87 gb|AEG71955.1| 2-haloacid halidohydrolase type II [Ralstonia sol... 325 3e-87 >ref|YP_003774554.1| HAD superfamily haloalkanoic acid dehalogenase [Herbaspirillum seropedicae SmR1] gb|ADJ62646.1| HAD superfamily haloalkanoic acid dehalogenase protein [Herbaspirillum seropedicae SmR1] Length = 222 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 221/222 (99%), Positives = 221/222 (99%) Query: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP Sbjct: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 Query: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA Sbjct: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 Query: 121 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 DDSQIMHNVEQLGA FHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL Sbjct: 121 DDSQIMHNVEQLGAAFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 Query: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL Sbjct: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 >ref|YP_004750979.1| Haloacid dehalogenase, type II [Collimonas fungivorans Ter331] gb|AEK60156.1| Haloacid dehalogenase, type II [Collimonas fungivorans Ter331] Length = 345 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 199/222 (89%), Positives = 211/222 (95%) Query: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 MSTFKPKFITFDCYGTLTRFQM+ETA+K+YADRV+ DMPFFLRQFAAYRLDEVLDQWKP Sbjct: 124 MSTFKPKFITFDCYGTLTRFQMAETAKKLYADRVAEADMPFFLRQFAAYRLDEVLDQWKP 183 Query: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 YEQVCKNAI RTC RWG++T DEE G FYKAVPTWGPH DVPA L+KVAKE PLVIFSNA Sbjct: 184 YEQVCKNAIRRTCERWGVKTTDEEMGTFYKAVPTWGPHPDVPAALAKVAKEIPLVIFSNA 243 Query: 121 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPE+VLHVSSSLRYDL Sbjct: 244 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEEVLHVSSSLRYDL 303 Query: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 MPA+DMGIKNKVFVARGHEPSTP+YGYTEI D+SGLPGV+GL Sbjct: 304 MPASDMGIKNKVFVARGHEPSTPYYGYTEISDLSGLPGVVGL 345 >ref|YP_002541869.1| haloacid dehalogenase, type II [Agrobacterium radiobacter K84] gb|ACM30272.1| haloacid dehalogenase, type II [Agrobacterium radiobacter K84] Length = 222 Score = 332 bits (852), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 151/222 (68%), Positives = 180/222 (81%) Query: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 M+TF+PK+ITFDCYGTLT FQM+E AQKIY +++ M F++ FAAYRLDE+L WKP Sbjct: 1 MTTFRPKYITFDCYGTLTNFQMAEAAQKIYGEQLDQPRMAQFIKNFAAYRLDEILGDWKP 60 Query: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 Y +V NA+ RTC + G+ DE+ + Y+ VP+WGPHADVPAGL+KVAKE PLVI SNA Sbjct: 61 YNEVLHNAVERTCRKNGVTFRDEDARSIYETVPSWGPHADVPAGLAKVAKEIPLVILSNA 120 Query: 121 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 + QIMHNVE+LGAPFH VFTAQQA AYKPR +AFEYM D L CNPEDV+H SSS RYDL Sbjct: 121 MNEQIMHNVEKLGAPFHAVFTAQQANAYKPRFKAFEYMFDMLGCNPEDVMHCSSSFRYDL 180 Query: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 M A D+GIK+KV+V RGHEP+ P+Y YTEIKDISGLPGV+GL Sbjct: 181 MSAYDLGIKHKVWVNRGHEPANPYYEYTEIKDISGLPGVVGL 222 >ref|YP_002822840.1| hydrolase [Sinorhizobium fredii NGR234] gb|AAQ87553.1| 2-haloacid halidohydrolase IVa [Sinorhizobium fredii NGR234] gb|ACP22087.1| putative hydrolase [Sinorhizobium fredii NGR234] Length = 222 Score = 330 bits (845), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 150/222 (67%), Positives = 180/222 (81%) Query: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 M++F+PK+ITFDCYGTLT FQM+E A+ +Y++++ M F+R FAAYRLDE+L WKP Sbjct: 1 MASFRPKYITFDCYGTLTNFQMAEAARDLYSEQLDEPRMQEFIRNFAAYRLDEILGDWKP 60 Query: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 Y +V N++ RTC R GI+ DE Y+ VP WGPHADVPAGL+KVAKE PLVI SNA Sbjct: 61 YAEVVHNSLERTCKRNGIKFRDEAARVVYERVPNWGPHADVPAGLAKVAKEIPLVILSNA 120 Query: 121 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 +SQIM NVE+LGAPFH V+TA+QAQAYKPR +AFEYMLD L C PED+LH SSS RYDL Sbjct: 121 MNSQIMSNVEKLGAPFHAVYTAEQAQAYKPRFKAFEYMLDMLGCGPEDILHCSSSFRYDL 180 Query: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 M A D+GIKNKV+V RGHEP+ P+YGYTEIKDISGLPGV+GL Sbjct: 181 MSAHDLGIKNKVWVNRGHEPANPYYGYTEIKDISGLPGVVGL 222 >ref|YP_546921.1| haloacid dehalogenase [Polaromonas sp. JS666] gb|ABE42023.1| haloacid dehalogenase, type II [Polaromonas sp. JS666] Length = 221 Score = 330 bits (845), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 149/220 (67%), Positives = 176/220 (80%) Query: 3 TFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKPYE 62 TF+PKFITFDCYGTLTRF+M + A++++ADR+ A M F+ F+AYR DEVL WKPY Sbjct: 2 TFRPKFITFDCYGTLTRFRMGDMAREMFADRIPAAQMELFVADFSAYRFDEVLGAWKPYA 61 Query: 63 QVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNADD 122 V +NA+ RTC +W I+ D E A+Y AVPTWGPHADVPAGLS++A + PLVI SNA + Sbjct: 62 DVLRNALNRTCKKWNIKCTDAEGRAYYDAVPTWGPHADVPAGLSRIADKIPLVILSNASN 121 Query: 123 SQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDLMP 182 QI NV++LGAPFH+VFTAQQAQAYKPRL+AFEYMLD L C PED+LHVSSSLRYDLM Sbjct: 122 DQIHKNVDKLGAPFHRVFTAQQAQAYKPRLQAFEYMLDQLGCGPEDILHVSSSLRYDLMS 181 Query: 183 AADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 A D+GIKNKVFV RGH P P Y Y EI+DI GLPGV+GL Sbjct: 182 ADDLGIKNKVFVNRGHGPGNPAYNYVEIQDIGGLPGVVGL 221 >ref|ZP_00946701.1| Probable 2-haloacid halidohydrolase IVa [Ralstonia solanacearum UW551] ref|YP_002257773.1| hydrolase protein [Ralstonia solanacearum IPO1609] gb|EAP70810.1| Probable 2-haloacid halidohydrolase IVa [Ralstonia solanacearum UW551] emb|CAQ36894.1| hydrolase protein [Ralstonia solanacearum MolK2] emb|CAQ59660.1| putative hydrolase protein [Ralstonia solanacearum IPO1609] Length = 221 Score = 326 bits (836), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 152/220 (69%), Positives = 174/220 (79%) Query: 3 TFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKPYE 62 TF+PK++TFDCYGTLTRF+M E ++++ADR+ A M F+ F AYR DEVL WKPYE Sbjct: 2 TFRPKYVTFDCYGTLTRFRMGELTRELFADRIPAAHMEPFIADFTAYRFDEVLGDWKPYE 61 Query: 63 QVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNADD 122 V KNA+ R C +W IQ D + A+Y AVPTW PHADVPAGL+KVA+E PLVI SNA D Sbjct: 62 VVLKNAVRRLCKKWRIQYYDADGQAYYDAVPTWDPHADVPAGLAKVAREIPLVILSNASD 121 Query: 123 SQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDLMP 182 QI NV LGAPFH+V+TAQQAQAYKPRL+AFEYMLD+L CNPEDVLHVSSSLRYDLM Sbjct: 122 DQIQKNVAMLGAPFHRVYTAQQAQAYKPRLKAFEYMLDSLGCNPEDVLHVSSSLRYDLMS 181 Query: 183 AADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 A D+GI NKVFV RGH P P Y YTEI+DI GLPGVLGL Sbjct: 182 ADDIGIVNKVFVNRGHGPGNPAYRYTEIQDIGGLPGVLGL 221 >ref|YP_004551835.1| haloacid dehalogenase type II [Sinorhizobium meliloti AK83] gb|AEG57712.1| haloacid dehalogenase, type II [Sinorhizobium meliloti AK83] Length = 222 Score = 325 bits (834), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 147/222 (66%), Positives = 178/222 (80%) Query: 1 MSTFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKP 60 MS F+PK+ITFDCYGTLT FQM+E A+ +Y++++ M F++ FAAYRLDE+L WKP Sbjct: 1 MSIFRPKYITFDCYGTLTNFQMAEAARDLYSEQLDEARMAEFIKNFAAYRLDEILGDWKP 60 Query: 61 YEQVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNA 120 Y +V N++ RTC R GI+ +E Y+ VPTWGPHADVPAGL++VAKE PLVI SNA Sbjct: 61 YAEVVHNSLERTCKRNGIEFREEAARMVYERVPTWGPHADVPAGLARVAKEIPLVILSNA 120 Query: 121 DDSQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDL 180 +SQIM NVE+LGAPFH V+TA+QAQAYKPR +AFEYMLD L C PED+LH SSS RYDL Sbjct: 121 MNSQIMSNVEKLGAPFHAVYTAEQAQAYKPRFKAFEYMLDMLGCGPEDILHCSSSFRYDL 180 Query: 181 MPAADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 M A D+GIKNKV+V RGHEP+ P+YGY EI DISGLPGV+GL Sbjct: 181 MSAHDLGIKNKVWVNRGHEPANPYYGYVEIADISGLPGVVGL 222 >ref|YP_004685539.1| haloacid dehalogenase type II [Cupriavidus necator N-1] gb|AEI77058.1| haloacid dehalogenase type II [Cupriavidus necator N-1] Length = 221 Score = 325 bits (834), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 150/220 (68%), Positives = 175/220 (79%) Query: 3 TFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKPYE 62 TF+PK++TFDCYGTLTRF+M E ++++ADR+ AE M F+ F+AYR DEVL W+PYE Sbjct: 2 TFRPKYVTFDCYGTLTRFRMGEMTRELFADRIPAEQMEQFIADFSAYRFDEVLGDWQPYE 61 Query: 63 QVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNADD 122 V KNA+ R C +W IQ D + +Y AVPTWGPH DVPAGL+KVAKE PLVI SNA D Sbjct: 62 VVLKNAVRRLCKKWKIQYFDTDGQQYYDAVPTWGPHEDVPAGLAKVAKEIPLVILSNASD 121 Query: 123 SQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDLMP 182 +QI NV LGAPFH+V+TAQQAQAYKPRL+AFEYMLD+L CNPEDVLHVSSSLRYDLM Sbjct: 122 AQIQKNVAMLGAPFHRVYTAQQAQAYKPRLKAFEYMLDSLGCNPEDVLHVSSSLRYDLMS 181 Query: 183 AADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 A D+GI NKVFV RGH P Y YTEI+DI GLPGV+GL Sbjct: 182 ADDIGIVNKVFVNRGHGPGNAAYRYTEIQDIGGLPGVVGL 221 >emb|CCA82055.1| 2-haloacid halidohydrolase type II [blood disease bacterium R229] Length = 221 Score = 325 bits (833), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 152/220 (69%), Positives = 174/220 (79%) Query: 3 TFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKPYE 62 TF+PK++TFDCYGTLTRF+M E ++++ADR+ AE M F+ F AYR DEVL W+PYE Sbjct: 2 TFRPKYVTFDCYGTLTRFRMGELTRELFADRIPAEHMEPFIADFTAYRFDEVLGDWQPYE 61 Query: 63 QVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNADD 122 V KNA+ R C +W IQ D + A+Y AVPTW PHADVPAGL+KVAKE PLVI SNA D Sbjct: 62 VVLKNAVRRLCKKWRIQYYDADGQAYYDAVPTWDPHADVPAGLAKVAKEIPLVILSNAAD 121 Query: 123 SQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDLMP 182 QI NV LGAPFH+V+TAQQAQAYKPRL+AFEYMLD+L CNPEDVLHVSSSLRYDLM Sbjct: 122 DQIQKNVAMLGAPFHRVYTAQQAQAYKPRLKAFEYMLDSLGCNPEDVLHVSSSLRYDLMS 181 Query: 183 AADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 A D+GI NKVFV RGH P P Y YTEI DI GLPGV+GL Sbjct: 182 ADDIGIVNKVFVNRGHGPGNPAYRYTEIPDIGGLPGVVGL 221 >gb|AEG71955.1| 2-haloacid halidohydrolase type II [Ralstonia solanacearum Po82] Length = 221 Score = 325 bits (833), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 151/220 (68%), Positives = 174/220 (79%) Query: 3 TFKPKFITFDCYGTLTRFQMSETAQKIYADRVSAEDMPFFLRQFAAYRLDEVLDQWKPYE 62 TF+PK++TFDCYGTLTRF+M E ++++ADR+ A M F+ F AYR DEVL WKPYE Sbjct: 2 TFRPKYVTFDCYGTLTRFRMGELTRELFADRIPAAHMEPFIADFTAYRFDEVLGDWKPYE 61 Query: 63 QVCKNAITRTCARWGIQTNDEETGAFYKAVPTWGPHADVPAGLSKVAKEFPLVIFSNADD 122 V KNA+ R C +W IQ D + A+Y AVPTW PHADVPAGL+KVA+E PLVI SNA D Sbjct: 62 VVLKNAVRRLCKKWRIQYYDADGQAYYDAVPTWDPHADVPAGLAKVAREIPLVILSNASD 121 Query: 123 SQIMHNVEQLGAPFHKVFTAQQAQAYKPRLRAFEYMLDNLNCNPEDVLHVSSSLRYDLMP 182 QI NV LGAPFH+V+TAQQAQAYKPRL+AFEYMLD+L CNPEDVLHVSSSLRYDLM Sbjct: 122 DQIQKNVAMLGAPFHRVYTAQQAQAYKPRLKAFEYMLDSLGCNPEDVLHVSSSLRYDLMS 181 Query: 183 AADMGIKNKVFVARGHEPSTPFYGYTEIKDISGLPGVLGL 222 A D+GI NKVFV RGH P P Y YTEI+DI GLPGV+GL Sbjct: 182 ADDIGIVNKVFVNRGHGPGNPAYRYTEIQDIGGLPGVVGL 221