BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0196.p (508 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773736.1| Fe-S oxidoreductase [Herbaspirillum seropedi... 1055 0.0 ref|ZP_05082367.1| Fe-S oxidoreductase [beta proteobacterium KB1... 825 0.0 ref|ZP_02736132.1| Fe-S oxidoreductase [Gemmata obscuriglobus UQ... 374 e-101 ref|YP_004672037.1| radical SAM domain-containing protein [Simka... 372 e-101 ref|YP_003797969.1| hypothetical protein NIDE2331 [Candidatus Ni... 368 1e-99 ref|YP_420154.1| Fe-S oxidoreductase [Magnetospirillum magneticu... 364 2e-98 ref|YP_001951043.1| radical SAM protein [Geobacter lovleyi SZ] >... 363 3e-98 ref|YP_003894587.1| Radical SAM domain-containing protein [Metha... 352 1e-94 ref|YP_003657093.1| radical SAM domain-containing protein [Arcob... 350 3e-94 gb|ABZ06234.1| putative Radical SAM superfamily protein [uncultu... 347 3e-93 >ref|YP_003773736.1| Fe-S oxidoreductase [Herbaspirillum seropedicae SmR1] gb|ADJ61828.1| Fe-S oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 508 Score = 1055 bits (2729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 504/508 (99%), Positives = 507/508 (99%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 MDALFV PDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS Sbjct: 1 MDALFVTPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME Sbjct: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 Query: 121 VLSHDCVDIVLLNEGVYALHNLLKSNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQSIVP 180 VLSHDCVDIVLLNEGVYALHNLLKSNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQSIVP Sbjct: 121 VLSHDCVDIVLLNEGVYALHNLLKSNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQSIVP 180 Query: 181 QERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDFCM 240 QERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDFCM Sbjct: 181 QERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDFCM 240 Query: 241 INIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLADLGVRTLRISDEMFFLNRKYYTP 300 INIVNRVDSSDGVSAAQSRGMRFWSPAWVAR+MRKLADLGVRTLRISDEMFFLNRKYYTP Sbjct: 241 INIVNRVDSSDGVSAAQSRGMRFWSPAWVARQMRKLADLGVRTLRISDEMFFLNRKYYTP 300 Query: 301 ILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKGSFK 360 ILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKGSFK Sbjct: 301 ILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKGSFK 360 Query: 361 EVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQALPGS 420 EVNIRDVCK+INDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQALPGS Sbjct: 361 EVNIRDVCKVINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQALPGS 420 Query: 421 PMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNPDYLSL 480 PMYYTAKKNGWALPDSYEGYAFLSYESQPLPTK+MSAAEVLKFRDEAWRTYFTNPDYLSL Sbjct: 421 PMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKYMSAAEVLKFRDEAWRTYFTNPDYLSL 480 Query: 481 VERRFGEQERKNVEDMTSIRLKRKLLGD 508 VERRFGEQERKNVEDMTSIRLKRKLLGD Sbjct: 481 VERRFGEQERKNVEDMTSIRLKRKLLGD 508 >ref|ZP_05082367.1| Fe-S oxidoreductase [beta proteobacterium KB13] gb|EDZ65054.1| Fe-S oxidoreductase [beta proteobacterium KB13] Length = 508 Score = 825 bits (2130), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/508 (76%), Positives = 444/508 (87%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 MD LFVNPDSS QAYQ LAK YSAIEPPTWSLLLAESCR+KG+ V ILDCDAERLT+ ++ Sbjct: 1 MDVLFVNPDSSKQAYQDLAKNYSAIEPPTWSLLLAESCRSKGYSVGILDCDAERLTIPKA 60 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 L RI+ KP+L+ VVYGQNPNSGTTSMIGA++LA++IK+ + + FVGSHTSALPM+ Sbjct: 61 LTRIDDCKPKLICFVVYGQNPNSGTTSMIGAISLAESIKEHNSEYTIAFVGSHTSALPMQ 120 Query: 121 VLSHDCVDIVLLNEGVYALHNLLKSNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQSIVP 180 VLS++CVDIVLLNEGVYALHNLLKSNL DLA IKGIGYKK+GP F P LN P+ +VP Sbjct: 121 VLSYNCVDIVLLNEGVYALHNLLKSNLKSDLANIKGIGYKKKGPGSFQTPHLNKPEIVVP 180 Query: 181 QERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDFCM 240 Q RMD DLPGYAWDLLP+ ++PLDLYRAHFWHA+F + KRTPFAAIYTSLGCSFGCDFCM Sbjct: 181 QSRMDNDLPGYAWDLLPFEKEPLDLYRAHFWHADFDYGKRTPFAAIYTSLGCSFGCDFCM 240 Query: 241 INIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLADLGVRTLRISDEMFFLNRKYYTP 300 INI+NR ++ DG+SAA SRGMRFWSP WV REM KL++LGV TLR+SDEMFFLN+K+Y P Sbjct: 241 INIINRNNNDDGISAADSRGMRFWSPDWVMREMEKLSNLGVETLRLSDEMFFLNKKFYEP 300 Query: 301 ILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKGSFK 360 ILQ +D F FNMW YSR+D+VR+D L+LFK+AGVNWLALGVEAGNQ VRQEVSKGSFK Sbjct: 301 ILQGIVDRGFKFNMWAYSRIDSVRKDYLDLFKKAGVNWLALGVEAGNQNVRQEVSKGSFK 360 Query: 361 EVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQALPGS 420 EVNIRDV K I ++DINIISNYIFGFP+DT +TMQETLDLALELNTEMANMYPCQALPGS Sbjct: 361 EVNIRDVSKTIQNSDINIISNYIFGFPEDTFDTMQETLDLALELNTEMANMYPCQALPGS 420 Query: 421 PMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNPDYLSL 480 PMY A +N W LPDSYEGYAFLSYES+PLPTK++S+AEVLKFRD+AW YFTNP+YL+L Sbjct: 421 PMYQKALQNNWKLPDSYEGYAFLSYESEPLPTKYLSSAEVLKFRDDAWNQYFTNPNYLNL 480 Query: 481 VERRFGEQERKNVEDMTSIRLKRKLLGD 508 VE +FG Q+RKN+EDM I+LKRKLLGD Sbjct: 481 VENKFGFQQRKNIEDMAKIKLKRKLLGD 508 >ref|ZP_02736132.1| Fe-S oxidoreductase [Gemmata obscuriglobus UQM 2246] Length = 529 Score = 374 bits (960), Expect = e-101, Method: Compositional matrix adjust. Identities = 204/512 (39%), Positives = 289/512 (56%), Gaps = 22/512 (4%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +D + +NP S Q YQ L +A+E P W+ L+A CR KG V I+D +AE L+ + Sbjct: 5 LDLVLINPSSRTQVYQSLGTELAAVENPVWAGLMANFCRQKGLSVEIIDAEAECLSPSEV 64 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 R+ L+P L +V YG P++ T M A+K A + V +G H +ALP Sbjct: 65 ADRVAYLRPVLTAVVAYGHQPSASTQIMTAVSRACSAVKVACSEQPVLLLGGHVAALPER 124 Query: 121 VLSHDCVDIVLLNEGVYALHNLLKS--NLADDLAQIKGIGYKKQGPA--GFAMPTLNAPQ 176 L + D V EGV+ L L+++ + D++ + G+ ++ G G A P L+ Sbjct: 125 TLREEEADFVAAGEGVHTLAGLVEALKSAVPDVSAVPGLYFRDNGRVRRGPAAPLLS--- 181 Query: 177 SIVPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGC 236 +D +LPG AWDLLP + YRAH WH HE R P+AA+YT+LGC + C Sbjct: 182 ------NLDTELPGIAWDLLP-----MPRYRAHNWHCLGGHE-RQPYAAVYTTLGCPYQC 229 Query: 237 DFCMINIVNRVDSSDGVSAAQS-RGMRFWSPAWVAREMRKLAD-LGVRTLRISDEMFFLN 294 FC I + + Q RFWS V ++ L + GVR ++I+DEMF LN Sbjct: 230 SFCCIQAPFKNGEVAPLGQKQPPNSYRFWSIDHVLGQIDTLVNKYGVRNIKIADEMFVLN 289 Query: 295 RKYYTPILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEV 354 +++ I I + N+W Y+RVDT++ L K AG NWLA+G+EAG VR +V Sbjct: 290 KRHVVGICDGIIARGYDLNIWAYTRVDTIKDGMLPKLKAAGFNWLAVGIEAGADRVRTDV 349 Query: 355 SKGSFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPC 414 K +F + + V + I A I++I NYIFG P+D TM+ TLDLAL+L E AN Y Sbjct: 350 DK-AFSQEQVYSVVREIQSAGISVIGNYIFGLPEDDHATMRATLDLALDLKCEFANFYSA 408 Query: 415 QALPGSPMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTN 474 A PGSP+Y TA + G LP + GY+ S +S PLPT+++ A EVLKFRD A+ Y+T+ Sbjct: 409 MAYPGSPLYATATQKGIPLPRRWTGYSQHSRDSLPLPTRYLPAREVLKFRDAAFTEYYTD 468 Query: 475 PDYLSLVERRFGEQERKNVEDMTSIRLKRKLL 506 P YL++VERRFG Q + + MT+I L+R LL Sbjct: 469 PGYLAMVERRFGAQSVEELRRMTAITLERDLL 500 >ref|YP_004672037.1| radical SAM domain-containing protein [Simkania negevensis Z] emb|CCB89546.1| radical SAM domain protein [Simkania negevensis Z] Length = 498 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 207/510 (40%), Positives = 295/510 (57%), Gaps = 29/510 (5%) Query: 2 DALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQSL 61 + LF+NP + YQ L SAIEPP+ + L A R KG AILD A L+ Q Sbjct: 3 EILFINPGAMHTVYQELGTSLSAIEPPSLAALFATYVRKKGAHAAILDAPALNLSPMQVA 62 Query: 62 VRIES-LKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 IE P LVV+VVYG P++ T +M+ A + IKK K+ G+H +ALP Sbjct: 63 QWIEKEYNPTLVVMVVYGFQPSASTQNMMAAGETCREIKKRNPSLKILMTGTHIAALPQR 122 Query: 121 VLSHDCVDIVLLNEG---VYALHNLLKSNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQS 177 + + VD V EG ++ ++ LK N + ++ + Y+K G A P P Sbjct: 123 TMEEEAVDFVCSGEGPQTIWGVYECLK-NGSTQFDRVPSLLYRK-GREIVATP--KGPL- 177 Query: 178 IVPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCD 237 E + E +PG AWDLLP ++ YRAH WH + ++R P+A+++TSLGC + C Sbjct: 178 ---LENLTEVMPGAAWDLLP-----MEKYRAHNWHCFENIDERQPYASLHTSLGCPYRCS 229 Query: 238 FCMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLAD-LGVRTLRISDEMFFLNRK 296 FC IN + R WSP V E+ L + GV+ ++ DEMF LN + Sbjct: 230 FCCIN-----------APFGGSSYRLWSPEAVISEIDLLVNRYGVKNIKFVDEMFVLNPR 278 Query: 297 YYTPILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSK 356 + I I+ ++G N+W Y+RVDTVR L+ KRAG+ WLALG+E+G++ VR V K Sbjct: 279 HVNSICDLLIERNYGLNIWAYARVDTVRDTFLDRLKRAGIRWLALGIESGSKHVRDGVEK 338 Query: 357 GSFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQA 416 G F +I V K I +A IN+I NYIFG PDDT ETM+ETLDLAL N E AN Y A Sbjct: 339 GRFGAEDILRVVKNIQNAGINVIGNYIFGLPDDTNETMKETLDLALSANCEFANFYCAMA 398 Query: 417 LPGSPMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNPD 476 PGS +Y A + GW LP + GY+ +YE+ PL T ++AAEVL+FRD+A+ TY+++P Sbjct: 399 YPGSKLYTLAIEKGWDLPTEWIGYSQHAYETLPLRTDTLTAAEVLEFRDKAFHTYYSDPR 458 Query: 477 YLSLVERRFGEQERKNVEDMTSIRLKRKLL 506 YLS ++ +FG++ ++++M I+L+RK++ Sbjct: 459 YLSFIQNKFGKKVLMHIKEMNKIKLRRKIV 488 >ref|YP_003797969.1| hypothetical protein NIDE2331 [Candidatus Nitrospira defluvii] emb|CBK42044.1| conserved protein of unknown function, Fe-S oxidoreductase [Candidatus Nitrospira defluvii] Length = 496 Score = 368 bits (944), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 207/509 (40%), Positives = 286/509 (56%), Gaps = 32/509 (6%) Query: 4 LFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQSLVR 63 L V P S VQ YQ L++ +AIEPP WS L+A R VAILD +AE L +Q+ R Sbjct: 2 LLVTPPSRVQVYQDLSRDLAAIEPPVWSGLIATFLRQHSCSVAILDAEAEGLNHQQTAER 61 Query: 64 IESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPMEVLS 123 I + PRL V V+YGQ P++ T M + + I D +G+H SALP L Sbjct: 62 IAVIAPRLAVFVIYGQQPSASTQCMPAGRTVCE-ILNTLADIPTLVMGTHPSALPKRTLL 120 Query: 124 HDCVDIVLLNEGVYALHNLLKSNLA--DDLAQIKGIGYKKQGPAGFAMPTLNAPQSIVPQ 181 + V EG + L+ + A L ++ G+ + ++G P NAP ++ Sbjct: 121 EEPYTYVCQGEGPSTILGLVIALRAPQHSLREVPGLWHMEKGE-----PVGNAPAQLL-- 173 Query: 182 ERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDFCMI 241 +D +LPG AWDLL + YRAH WH + E RTP+A++ TSLGC F C FC I Sbjct: 174 TNLDRELPGQAWDLLD-----MTRYRAHNWHCFGNLEARTPYASLQTSLGCPFTCSFCCI 228 Query: 242 NIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLA-DLGVRTLRISDEMFFLNRKYYTP 300 N S + +R WSP V ++ +L D G+ ++I DEMF LNR++ Sbjct: 229 N-----------SPFGTPMLRTWSPDNVIGQIDRLVRDYGISNIKIPDEMFVLNRRHVIG 277 Query: 301 ILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKGSFK 360 I + I+ + N+W Y+RVDTV+ + L RAG WL LG+E+G+Q VR V KG F Sbjct: 278 ICDRIIERGYRLNIWAYARVDTVQDEVLAKLARAGFTWLGLGIESGSQHVRDGVEKGRFG 337 Query: 361 EVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQALPGS 420 E +I I I++ +NYIFG PDDT E+M+ TLDLAL LNTE AN Y A PGS Sbjct: 338 ERDIVATVDKIRSYGIHVAANYIFGLPDDTMESMRATLDLALTLNTEWANFYCAMAYPGS 397 Query: 421 PMYYTAKKNGWALPD-----SYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNP 475 P+Y AK+ WALPD + GYA +Y+ +PLPT H++A +VL FRD A+ YFT+P Sbjct: 398 PLYTQAKQKQWALPDDQGGPGWIGYAQHAYDCRPLPTDHLTATQVLAFRDRAFIEYFTHP 457 Query: 476 DYLSLVERRFGEQERKNVEDMTSIRLKRK 504 YL+++ R FG +V DM +++R+ Sbjct: 458 QYLNMLHRTFGPHVATHVADMCRRQVRRR 486 >ref|YP_420154.1| Fe-S oxidoreductase [Magnetospirillum magneticum AMB-1] dbj|BAE49595.1| Fe-S oxidoreductase [Magnetospirillum magneticum AMB-1] Length = 498 Score = 364 bits (934), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 205/513 (39%), Positives = 296/513 (57%), Gaps = 31/513 (6%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +D L V P S ++ YQ L+ ++AIEPP W L+AE R++G VA+LD +A +LT EQ+ Sbjct: 4 LDVLIVTPPSRLEVYQNLSNDFAAIEPPVWGGLIAEFLRSRGVAVAMLDAEALKLTHEQT 63 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAI-KKAQLDTKVCFVGSHTSALPM 119 RI + +P + ++ +YGQ P++ T M A A+ + +K + +G+H SALP Sbjct: 64 AERIVAAQPLVTLITIYGQQPSASTQCMPAATKTARLVAEKGKGRLATIALGTHPSALPK 123 Query: 120 EVLSHDCVDIVLLNEGVYALHNLLKSNLAD-DLAQIKGIGYKKQGPAGFAMPTLNAPQSI 178 L + V EG Y L L+++ +D DL + G+ Y+ + N P + Sbjct: 124 RTLEEEPFTYVCQGEGPYTLLELVRALKSDGDLGAVPGLWYRDRD----GKVRSNTPAPL 179 Query: 179 VPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDF 238 + +D +LP A DLL + LYRAH WH + + R +A++ TSLGC F C F Sbjct: 180 ISD--LDAELPRQALDLLD-----MSLYRAHNWHCFGNLDSRDSYASLQTSLGCPFKCSF 232 Query: 239 CMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLA-DLGVRTLRISDEMFFLNRKY 297 C IN V+ +R WSP V ++ ++ GVR ++I DEMF LN K+ Sbjct: 233 CCINAPFGVNK-----------IRTWSPDNVIAQIDEMVLKYGVRNIKIPDEMFVLNPKH 281 Query: 298 YTPILQQAIDEDFGFNMWTYSRVDTVRRDA-LELFKRAGVNWLALGVEAGNQMVRQEVSK 356 I + I+ +G N+W Y+RVDT+ DA L+ K AG+NWL LG+E+G++ VR V+K Sbjct: 282 VLGICDKLIERGYGLNLWAYARVDTLNDDAMLDKLKAAGINWLGLGIESGSKYVRDGVTK 341 Query: 357 GSFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQA 416 G F ++I V + + D I + +NYIFG PDDT E+MQETLDLA+ LNTE AN Y A Sbjct: 342 GRFGNLDISAVVRRVRDKGIYVGANYIFGLPDDTLESMQETLDLAISLNTEWANFYCAMA 401 Query: 417 LPGSPMYYTAKKNGWALPD-----SYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTY 471 PGS +Y AK+ LPD + GYA S++ PLPT +SA +VL FRD+A+ Y Sbjct: 402 YPGSHLYEMAKEQNLPLPDDAGGPGWIGYAQHSFDCLPLPTATLSAQQVLDFRDQAFTRY 461 Query: 472 FTNPDYLSLVERRFGEQERKNVEDMTSIRLKRK 504 FTNPDYL ++ + FGE+ +V DMT L R+ Sbjct: 462 FTNPDYLEMIRKTFGERAMAHVRDMTGHTLPRR 494 >ref|YP_001951043.1| radical SAM protein [Geobacter lovleyi SZ] gb|ACD94523.1| Radical SAM domain protein [Geobacter lovleyi SZ] Length = 489 Score = 363 bits (933), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 203/511 (39%), Positives = 295/511 (57%), Gaps = 28/511 (5%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +D + +NP Q YQGL +AIEPP W ++A R +G VAI+D +AE ++ Sbjct: 4 VDIVLINPGDRKQVYQGLGVELAAIEPPFWVAVIAAYLRQEGFRVAIIDANAENSAPAET 63 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 R +L+P L +VVYG +P++ T +M A A+ +A+ A+ +V G H SALP Sbjct: 64 AQRAAALQPLLSCVVVYGSHPSASTQNMTIAGAICRALT-AESAGRVALSGLHPSALPER 122 Query: 121 VLSHDCVDIVLLNEGVYALHNLLK--SNLADDLAQIKGIGYKKQGPAGFAMPTLNAPQSI 178 + + +D V+ EG L LL + A DL+++ G+ Y++ T AP Sbjct: 123 TMREEAIDFVVEGEGPDTLRTLLGELAAPAPDLSRVPGLWYRE---GELLRSTPRAPLI- 178 Query: 179 VPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGCDF 238 +D LP AWDLLP + +YRAH WH R+P+ A+YTSLGC + C F Sbjct: 179 ---SDLDRYLPIAAWDLLP-----MHVYRAHNWHCFDDIGHRSPYGAVYTSLGCPYNCIF 230 Query: 239 CMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLA-DLGVRTLRISDEMFFLNRKY 297 C IN + G+R+ SP V E+ LA D GV+ L+I DE+F L ++ Sbjct: 231 CCIN-----------APFGKPGIRYRSPERVVEEIALLANDYGVKNLKIVDELFVLKEQH 279 Query: 298 YTPILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVSKG 357 Y I+ I+ N+W Y+RVDT++ L K+AG+NWLALG+E+ + VR K Sbjct: 280 YMAIVDGIIERGLDLNIWAYARVDTIKTANLARMKQAGINWLALGIESASPDVRDGADK- 338 Query: 358 SFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQAL 417 + +I++V + I + I II N+IFG PDDTRETM+ETL LA+ELN E N Y A Sbjct: 339 RMQARDIKEVVRSIREQGIRIIGNFIFGLPDDTRETMEETLQLAMELNCEFINFYCAMAY 398 Query: 418 PGSPMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNPDY 477 PGS +Y A + GW LP ++ G++ Y+ PLPT+ ++A EVL+FRD+A+ YF NP Y Sbjct: 399 PGSRLYDLAVEQGWELPVAWHGFSQHGYDMLPLPTRTLAAREVLQFRDDAFHRYFANPVY 458 Query: 478 LSLVERRFGEQERKNVEDMTSIRLKRKLLGD 508 L +VE+ FG R+++ +M++ RLKRKLLG+ Sbjct: 459 LDMVEQAFGAGVREHLVEMSATRLKRKLLGN 489 >ref|YP_003894587.1| Radical SAM domain-containing protein [Methanoplanus petrolearius DSM 11571] gb|ADN36149.1| Radical SAM domain protein [Methanoplanus petrolearius DSM 11571] Length = 490 Score = 352 bits (902), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 202/509 (39%), Positives = 281/509 (55%), Gaps = 30/509 (5%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +D L +NP + Q Y L S IEPP W LLA R G+ V ILD +AE L+ ++ Sbjct: 5 LDLLLINPGARNQVYGKLGDSLSGIEPPLWCGLLAGFIRENGYSVRILDAEAENLSPHET 64 Query: 61 LVRIESLKPRLVVLVVYGQNPN-SGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPM 119 RI LKP L ++ G NP+ S T M A KA+K+ + K G H SALP Sbjct: 65 AKRIADLKPILAGIIALGSNPSASSTPKMTAAGETLKALKQENPNIKTLLGGLHPSALPE 124 Query: 120 EVLSHDCVDIVLLNEGVYALHNLLKSNLADDLAQ---IKGIGYKKQGPAGFAMPTLNAPQ 176 L+ + VD V EG + LLK D+ ++ + G+ YKK+G L+ P Sbjct: 125 RTLTEEEVDFVCQGEGFETVLQLLKILRTDNESEDFIVNGLWYKKKGS------VLSNPP 178 Query: 177 SIVPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGC 236 + P + +DE LP AWDLLP +D+YRAH WH + KR +A IYTSLGC F C Sbjct: 179 A-EPIQNLDE-LPFVAWDLLP-----MDIYRAHNWHCFSNLNKRGHYAVIYTSLGCPFNC 231 Query: 237 DFCMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLAD-LGVRTLRISDEMFFLNR 295 ++C I+ + G+RF SP V E+ L + L+I DE+F LN Sbjct: 232 NYCNIH-----------ALYGKPGIRFRSPEKVIEEIDFLVKKYQIENLKIIDELFVLNE 280 Query: 296 KYYTPILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQEVS 355 K + ID ++ N+W Y+RVDT+ K AG+NWLA G+E+ ++ VR V+ Sbjct: 281 KRVKKLCDLIIDREYNLNIWAYARVDTINESLANKMKSAGINWLAFGIESASKDVRDGVT 340 Query: 356 KGSFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMYPCQ 415 K F + I +I A I I+ N+IFG PDD +TMQETLDLA ELN E N Y Sbjct: 341 K-RFDQNKIEKAIEIAQKAGIFIMGNFIFGLPDDNNQTMQETLDLAKELNLEYINFYTAM 399 Query: 416 ALPGSPMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRTYFTNP 475 A PGS +Y+ A +NG LPD + G+A E+ PLPTK++S+AEVL+FRD A+ Y +NP Sbjct: 400 AYPGSKLYFEAIQNGIKLPDKWHGFAQYGEETLPLPTKYLSSAEVLRFRDYAFDNYLSNP 459 Query: 476 DYLSLVERRFGEQERKNVEDMTSIRLKRK 504 YL ++ +FG + ++++ M ++KRK Sbjct: 460 KYLKMIREKFGIEVEEHIKKMLEHKIKRK 488 >ref|YP_003657093.1| radical SAM domain-containing protein [Arcobacter nitrofigilis DSM 7299] gb|ADG94586.1| Radical SAM domain protein [Arcobacter nitrofigilis DSM 7299] Length = 486 Score = 350 bits (898), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 193/518 (37%), Positives = 294/518 (56%), Gaps = 43/518 (8%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +D +F+NP +Q L K +AIEPP +L A + + V ILD +AE +T E++ Sbjct: 2 IDIIFINPGDRKVIFQDLGKDITAIEPPYLTLSFATYLKNQNINVKILDANAENITPEET 61 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDTKVCFVGSHTSALPME 120 +++ L P+LV L+VYG P++ T +M + +A IK + ++ + G H SALP Sbjct: 62 AQKVKELNPKLVALIVYGNQPSASTQNMSISGKIATTIK-SIINVPIVMGGLHPSALPKR 120 Query: 121 VLSHDCVDIVLLNEGVYALHNLLKSNLAD-DLAQIKGIGYK-----KQGPAGFAMPTLNA 174 L + +D V+ E L L+K + D ++++G+ Y K P G + L Sbjct: 121 TLEEEDIDFVIEGEEQIPLQELIKEIKNNKDYSKVEGLWYYESNEIKNNPKGKLISNL-- 178 Query: 175 PQSIVPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSF 234 D+ +P WD+LP +D YRAH WH E R P+A+IYTSLGC + Sbjct: 179 ----------DDYMPIADWDMLP-----MDKYRAHNWHCFDDIENRMPYASIYTSLGCPY 223 Query: 235 GCDFCMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKL-ADLGVRTLRISDEMFFL 293 C FC IN + +R+ SP + E+ L ++ ++ DEMF L Sbjct: 224 KCTFCCIN-----------APFGKSTIRYRSPEIIVNELELLNTKYKIKNIKFIDEMFVL 272 Query: 294 NRKYYTPILQQAIDEDFGFNMWTYSRVDTVRRDALELFKRAGVNWLALGVEAGNQMVRQE 353 + ++Y I+ I+++ N+W Y+RVDT++ L+ K+AG+NWLALG+E+ N VR Sbjct: 273 HEEHYMKIVDLIIEKNLDLNIWCYARVDTIKPYTLKRMKQAGINWLALGIESANPNVRD- 331 Query: 354 VSKGSFKEVNIRDVCKIIND---ADINIISNYIFGFPDDTRETMQETLDLALELNTEMAN 410 G+ K++ ++D+ + +ND I +I NYIFG DDT E+MQETLD+A ELN E AN Sbjct: 332 ---GASKKMRVKDIKQQVNDIQSVGIRVIGNYIFGLQDDTIESMQETLDMAKELNCEFAN 388 Query: 411 MYPCQALPGSPMYYTAKKNGWALPDSYEGYAFLSYESQPLPTKHMSAAEVLKFRDEAWRT 470 Y A PGSP+Y A K LPD + GY+ SY QPLP+K+++A E++KFRDEA+ Sbjct: 389 FYCAMAYPGSPLYNIALKESLELPDVWHGYSQHSYVMQPLPSKYVTAKEIVKFRDEAFHE 448 Query: 471 YFTNPDYLSLVERRFGEQERKNVEDMTSIRLKRKLLGD 508 YF + YL+++E +FG +K++ ++T RLKRK+L D Sbjct: 449 YFESSTYLNMLENKFGVDVKKHMVEITQTRLKRKILDD 486 >gb|ABZ06234.1| putative Radical SAM superfamily protein [uncultured marine microorganism HF4000_007I05] Length = 505 Score = 347 bits (889), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 192/517 (37%), Positives = 290/517 (56%), Gaps = 36/517 (6%) Query: 1 MDALFVNPDSSVQAYQGLAKVYSAIEPPTWSLLLAESCRAKGHEVAILDCDAERLTLEQS 60 +DAL + P S ++ YQGL+ Y+AIEPP WS+L+A +G+ ILD +AE LT +Q+ Sbjct: 4 VDALLITPPSRIEVYQGLSDDYAAIEPPVWSMLIANYLIQRGYNAQILDAEAENLTHDQT 63 Query: 61 LVRIESLKPRLVVLVVYGQNPNSGTTSMIGALALAKAIKKAQLDT-KVCFVGSHTSALPM 119 +I + P+L V +VYGQ P++ T M G + + ++ K VG+H SALP Sbjct: 64 AEKILKINPKLAVFMVYGQQPSASTQCMPGGKRTCDKLNELSSNSIKTIVVGTHASALPK 123 Query: 120 EVLSHDCVDIVLLNEGVYALHNLLKS--NLADDLAQIKGIG-YKKQGPAGFAMPTLNAPQ 176 + L + + V EG + L+++ N L +I G+ Y K F AP Sbjct: 124 KTLEEEPYNFVCQGEGPITITKLIENIKNGKFKLEEIPGLWFYDKDKEIKFNQ---RAPM 180 Query: 177 SIVPQERMDEDLPGYAWDLLPYREKPLDLYRAHFWHAEFSHEKRTPFAAIYTSLGCSFGC 236 + +D LPG AW LL ++ Y+AH WH E R +A++ TSLGC F C Sbjct: 181 FL----NLDLSLPGQAWKLLDMKK-----YKAHNWHTFGKLETRNRYASLQTSLGCPFKC 231 Query: 237 DFCMINIVNRVDSSDGVSAAQSRGMRFWSPAWVAREMRKLA-DLGVRTLRISDEMFFLNR 295 FC IN + + +RFW+P + +++ + D + ++I DEMF LN Sbjct: 232 TFCCIN-----------APFEKNTIRFWTPKHIINQIKIIVEDYNIFNIKIPDEMFVLNP 280 Query: 296 KYYTPILQQAIDEDFG--FNMWTYSRVDTVR-RDALELFKRAGVNWLALGVEAGNQMVRQ 352 K + I + I+ +G N W Y+R+DT+ + L+ ++G WLALG+E+ ++ VR Sbjct: 281 KQVSEICDEIINSGYGSKLNFWAYARIDTLEDNEMLKKMIKSGFKWLALGIESSSKHVRD 340 Query: 353 EVSKGSFKEVNIRDVCKIINDADINIISNYIFGFPDDTRETMQETLDLALELNTEMANMY 412 V KG F +I D+ K + D + +NYIFG PDD ++M+ETLDL+L +N+E AN Y Sbjct: 341 GVVKGRFNNYDIEDIVKKVRDMGFFVGANYIFGLPDDNNDSMKETLDLSLRINSEWANFY 400 Query: 413 PCQALPGSPMYYTAKKNGWALPD-----SYEGYAFLSYESQPLPTKHMSAAEVLKFRDEA 467 A PGS +Y AKK GW LPD + GY+ +YES PL T+H+ +EVL+FRD+A Sbjct: 401 SGMAYPGSQLYPMAKKKGWTLPDDKVGPGWIGYSQHAYESLPLRTEHVKGSEVLEFRDKA 460 Query: 468 WRTYFTNPDYLSLVERRFGEQERKNVEDMTSIRLKRK 504 + YF + DYLS++ + FG++ ++ M S +LKRK Sbjct: 461 FDIYFKSSDYLSMITKTFGKETTDHITKMASHKLKRK 497