BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0001.p (247 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [Herbaspirillu... 478 e-133 ref|YP_001770730.1| hydratase/decarboxylase [Methylobacterium sp... 196 2e-48 ref|YP_004618283.1| 2-keto-4-pentenoate hydratase [Ramlibacter t... 196 3e-48 ref|YP_002942039.1| 2-oxopent-4-enoate hydratase [Variovorax par... 176 4e-42 ref|YP_729041.1| 2-oxopent-4-enoate hydratase [Ralstonia eutroph... 174 8e-42 ref|YP_002363660.1| fumarylacetoacetate (FAA) hydrolase [Methylo... 127 1e-27 ref|YP_004866897.1| 2-oxopent-4-enoate hydratase [Gluconacetobac... 123 2e-26 ref|ZP_07031697.1| conserved hypothetical protein [Acidobacteriu... 119 4e-25 ref|ZP_06834560.1| putative 2-keto-4-pentenoate hydratase-like p... 119 6e-25 ref|ZP_08316368.1| 2-keto-4-pentenoate hydratase 2 [Gluconacetob... 117 2e-24 >ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [Herbaspirillum seropedicae SmR1] gb|ADJ64831.1| 2-keto-4-pentenoate hydratase protein [Herbaspirillum seropedicae SmR1] Length = 247 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 243/247 (98%), Positives = 244/247 (98%) Query: 1 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT 60 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT Sbjct: 1 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT 60 Query: 61 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL 120 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL Sbjct: 61 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL 120 Query: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQVS 180 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPD+LSLRQIEASLSIDGQQVS Sbjct: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDLLSLRQIEASLSIDGQQVS 180 Query: 181 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGA 240 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCT MARGGRRVEAALHGIGA Sbjct: 181 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCTGLQMARGGRRVEAALHGIGA 240 Query: 241 VWLDFAG 247 VWLDFAG Sbjct: 241 VWLDFAG 247 >ref|YP_001770730.1| hydratase/decarboxylase [Methylobacterium sp. 4-46] gb|ACA18296.1| Hydratase/decarboxylase [Methylobacterium sp. 4-46] Length = 238 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 127/241 (52%), Positives = 151/241 (62%), Gaps = 6/241 (2%) Query: 7 TQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPL 66 LLL AR G + WR +A D AYAVQDA RLGPVGGWKVG+ P AP Sbjct: 2 ADLLLAAR-GGARVPWRAVAPADPAGAYAVQDAVAARLGPVGGWKVGAANPEAVPNAAPC 60 Query: 67 PASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVV 126 PA+ + SG L GP+WR+RG+E E+ LR+GRD D AEL +A+LP IEVV Sbjct: 61 PAACLRGSGAVLDGPEWRLRGIEAEVGLRLGRDLDGAAP---RAELAAAVEAVLPVIEVV 117 Query: 127 ETRLGHPPCTNPWAGMADLQSHGALVTGAPQAL-PDVLSLRQIEASLSIDGQQVSHTTGG 185 ETRL +P A +ADLQSHGAL+ G P L P L LR + A LS+DG+ V+ T GG Sbjct: 118 ETRLDGWGEADPLARLADLQSHGALILGPPSRLDPAALDLRTVAADLSVDGRAVAATRGG 177 Query: 186 NPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAVWLD 244 NPA D+W LL WLA H A RG+P RAGQ++TTGSCT G RV A L GIGAV + Sbjct: 178 NPAEDVWRLLAWLADHAAARGRPLRAGQVVTTGSCTGMLFCAQGTRVAAELAGIGAVAMR 237 Query: 245 F 245 F Sbjct: 238 F 238 >ref|YP_004618283.1| 2-keto-4-pentenoate hydratase [Ramlibacter tataouinensis TTB310] gb|AEG92264.1| 2-keto-4-pentenoate hydratase-like protein [Ramlibacter tataouinensis TTB310] Length = 250 Score = 196 bits (498), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 115/245 (46%), Positives = 146/245 (59%), Gaps = 2/245 (0%) Query: 3 IDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPG 62 + A + L +AR G + + A AYA+QDA L LGP GGWKVG+ P Sbjct: 6 FETAGRALAEARRGGGKVPHASIPAAQATDAYAIQDATLRVLGPHGGWKVGAASAQAMPN 65 Query: 63 CAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPA 122 CAPLP+SG+LP G TLA P +RG+E+E+A+R+GRD P ++ AE+ DA+LPA Sbjct: 66 CAPLPSSGLLPGGATLARPGAALRGIELEVAVRLGRDLLPDGRVLPAAEIVAAVDALLPA 125 Query: 123 IEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQAL-PDVLSLRQIEASLSIDGQQVSH 181 IEVV+TRL + P +ADLQSH LVTGA AL P L L + L DG + Sbjct: 126 IEVVDTRLAEGRQSPPPVQLADLQSHDVLVTGAASALKPARLDLASLRCRLWFDGSPAAD 185 Query: 182 TTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGA 240 T GG+P D+W LL WLA+HC RG P RAGQ++TTGSCT A RV + G+G Sbjct: 186 TVGGHPTRDIWSLLAWLAVHCEARGLPLRAGQVVTTGSCTGLLFAPASARVRGEIDGLGE 245 Query: 241 VWLDF 245 V L F Sbjct: 246 VALSF 250 >ref|YP_002942039.1| 2-oxopent-4-enoate hydratase [Variovorax paradoxus S110] gb|ACS16773.1| 2-oxopent-4-enoate hydratase [Variovorax paradoxus S110] Length = 254 Score = 176 bits (445), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 102/215 (47%), Positives = 130/215 (60%), Gaps = 2/215 (0%) Query: 33 AYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEVEL 92 AYA+QDA L +GPVGGWKVG++ G P CAPLPA G+LP G L GP WR+RG+E E+ Sbjct: 40 AYAIQDATLAAIGPVGGWKVGARAPGQEPTCAPLPAEGLLPGGVRLQGPAWRLRGIEAEV 99 Query: 93 ALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALV 152 ++G D P A++ +++LP IEVVETRL + A +ADL SHGALV Sbjct: 100 GFQLGADLPPRAAPYTLADVAGAVESVLPVIEVVETRLADWFGAHANALLADLLSHGALV 159 Query: 153 TGAPQAL-PDVLSLRQIEASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRA 210 G Q P L++ EA+L DGQ V+HT G +P+ D LL WLA HCA RG +A Sbjct: 160 LGQRQPFAPAWFDLQRAEAALHFDGQTVAHTVGEHPSPDAGALLVWLANHCAARGAGLKA 219 Query: 211 GQIITTGSCTCHTMARGGRRVEAALHGIGAVWLDF 245 GQI+T+GSCT A G V + + V L F Sbjct: 220 GQIVTSGSCTGMLFASAGTAVRVEIGELAPVALSF 254 >ref|YP_729041.1| 2-oxopent-4-enoate hydratase [Ralstonia eutropha H16] emb|CAJ95676.1| 2-Oxopent-4-enoate hydratase [Ralstonia eutropha H16] Length = 254 Score = 174 bits (442), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 109/243 (44%), Positives = 144/243 (59%), Gaps = 4/243 (1%) Query: 5 AATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCA 64 A Q+L AR G +D L +YAVQDA L +GP+GGWKVG++G G P CA Sbjct: 12 ALAQMLCVARTRGERVDPALLHPLAHGGSYAVQDATLAMIGPIGGWKVGARGPGQEPACA 71 Query: 65 PLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIE 124 PLPA+G+LP G L GP WR+RG+EVE+A +G P + + ++ ++PAIE Sbjct: 72 PLPAAGLLPDGVGLRGPAWRLRGIEVEVAFVLGASLPPRGRPYERRDIEAALALVVPAIE 131 Query: 125 VVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALP-DVLSLRQIEASLSIDGQQVSHTT 183 VVE+RL A +ADL SHGALVTG P + L L +EA+L + V+HT Sbjct: 132 VVESRLEDWLGAGSDAQLADLLSHGALVTGRPTPFAREWLHLGALEATLRFGRRTVAHTV 191 Query: 184 GGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIG--A 240 GG+ + D+ LL WLA HCA RG +AGQ++TTG+CT A G RV A + G+G A Sbjct: 192 GGHQSPDVGALLVWLANHCAARGAGLKAGQVVTTGTCTGLLFASAGTRVSAGIEGLGTLA 251 Query: 241 VWL 243 W Sbjct: 252 AWF 254 >ref|YP_002363660.1| fumarylacetoacetate (FAA) hydrolase [Methylocella silvestris BL2] gb|ACK52298.1| fumarylacetoacetate (FAA) hydrolase [Methylocella silvestris BL2] Length = 260 Score = 127 bits (320), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 90/250 (36%), Positives = 122/250 (48%), Gaps = 12/250 (4%) Query: 1 MHIDAATQL---LLDARHSG-----LALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKV 52 M D AT L L+DA+ +G L ++ R + E AY VQ A RLGP+ GWKV Sbjct: 8 MECDEATALAALLIDAQETGASIGELPVNAR---ISSREEAYRVQSALARRLGPIVGWKV 64 Query: 53 GSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAEL 112 G K APL A+ PSG +R+ LE ELA R P + E+ Sbjct: 65 GRKAADAPASRAPLFANRTHPSGAKFERDAFRLFLLEAELAFRFRSGLPPIGRPYARDEV 124 Query: 113 RTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASL 172 T ++ A E++++R P P +ADL SHGA+V G+ +PD S SL Sbjct: 125 LTAISEVVVAFEIIDSRFRDWPTVAPELLLADLLSHGAMVVGSGVPMPDHPSFINTSVSL 184 Query: 173 SIDGQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRV 231 ++D + + GGNPA D+ L+ WLA G RAG ++TTGS TC G Sbjct: 185 AVDDRMIISRDGGNPAGDVIDLVVWLANDLLDVGAALRAGDLVTTGSYTCMERLAPGSSA 244 Query: 232 EAALHGIGAV 241 EA+ G+G V Sbjct: 245 EASFAGVGTV 254 >ref|YP_004866897.1| 2-oxopent-4-enoate hydratase [Gluconacetobacter xylinus NBRC 3288] dbj|BAK82527.1| 2-oxopent-4-enoate hydratase [Gluconacetobacter xylinus NBRC 3288] Length = 253 Score = 123 bits (309), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 82/219 (37%), Positives = 108/219 (49%), Gaps = 1/219 (0%) Query: 24 RLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQW 83 RL E AY +QD LGP+ GWKVG++ P CAP+ AS + G T+ Sbjct: 30 RLVPLSIEEAYRIQDEVAQHLGPIVGWKVGAQTPSSEPFCAPIHASTLFSDGATVPHGLC 89 Query: 84 RMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMA 143 R G+E E+A R + P E+ E+ I P IE+++TR P + + A Sbjct: 90 RHLGVEAEIAYRFAQALPPRERDWTRDEVMDRIGTIHPVIEILDTRFETPGSQHKFLHTA 149 Query: 144 DLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCA 202 D QSHGAL+ G A + + LSI+ + V GGN A D LL WLA H + Sbjct: 150 DQQSHGALIVGPGSAEWQKYNPVEEHVRLSINDETVVEHVGGNSAGDPLRLLVWLANHAS 209 Query: 203 RRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAV 241 RR AG I+TTGS T G RV A+ GIG+V Sbjct: 210 RRNLGIDAGSIVTTGSTTGTIFVDHGARVSASFSGIGSV 248 >ref|ZP_07031697.1| conserved hypothetical protein [Acidobacterium sp. MP5ACTX8] gb|EFI55923.1| conserved hypothetical protein [Acidobacterium sp. MP5ACTX8] Length = 254 Score = 119 bits (298), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 88/248 (35%), Positives = 118/248 (47%), Gaps = 4/248 (1%) Query: 2 HIDAATQLLLDARHSG--LALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGG 59 ++AA LLLDAR + +A L + + YAVQD G +GGWK+G+ Sbjct: 8 QLEAAANLLLDARRTNTPIADLPEELRPTNLDEVYAVQDMIAAAYGEIGGWKIGAPNPEA 67 Query: 60 TPGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAI 119 TP AP+PA V SG+ + G +W RGLE E+A VG D P E + Sbjct: 68 TPMYAPMPAQWVATSGSVIDGDRW-YRGLEAEIAFLVGEDLPPRATPYSREEAVAAMASC 126 Query: 120 LPAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQV 179 P IEV+E L + + +ADLQ HG + G + + +L++DG Sbjct: 127 HPVIEVLEPGLSDSTKADRNSTLADLQMHGGFIYGPAFDNWQSVDFNTEKVTLAVDGVIR 186 Query: 180 SHTTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGI 238 TG N + DL LL WLA A R R GQ ITTGS T +T A G + + Sbjct: 187 VERTGSNTSGDLVKLLPWLANEGAARTGGLRKGQWITTGSWTGNTQASAGSAADVVFSTV 246 Query: 239 GAVWLDFA 246 G+V L FA Sbjct: 247 GSVSLRFA 254 >ref|ZP_06834560.1| putative 2-keto-4-pentenoate hydratase-like protein [Gluconacetobacter hansenii ATCC 23769] gb|EFG84264.1| putative 2-keto-4-pentenoate hydratase-like protein [Gluconacetobacter hansenii ATCC 23769] Length = 253 Score = 119 bits (297), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 81/217 (37%), Positives = 107/217 (49%), Gaps = 11/217 (5%) Query: 31 ETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEV 90 E AY +QD LGP+ GWKVG++ P CAP+ AS + G T+ R G+E Sbjct: 37 EDAYRIQDEVAQHLGPIVGWKVGAQTPSSEPFCAPIHASTLFNDGATVPHGLCRHLGVEA 96 Query: 91 ELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGA 150 E+A R + P E+ E+ I P IE+++TR P + + AD QSHGA Sbjct: 97 EIAYRFAQALPPRERDWTRDEVMDRIGTIHPVIEILDTRFETPGSQHKFLHTADQQSHGA 156 Query: 151 LVTGAPQALPDVLSLRQIE-----ASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCARR 204 L+ G P V R+ LSI+ + V+ GGN A D LL WLA H +RR Sbjct: 157 LIVG-----PGVAEWRKYNPVVEHVRLSINDETVAEHVGGNSAGDPLRLLVWLANHASRR 211 Query: 205 GQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAV 241 AG I+TTGS T G RV A+ IG+V Sbjct: 212 NLGIDAGSIVTTGSTTGTIFVDHGARVSASFPAIGSV 248 >ref|ZP_08316368.1| 2-keto-4-pentenoate hydratase 2 [Gluconacetobacter sp. SXCC-1] gb|EGG77115.1| 2-keto-4-pentenoate hydratase 2 [Gluconacetobacter sp. SXCC-1] Length = 253 Score = 117 bits (292), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 85/215 (39%), Positives = 105/215 (48%), Gaps = 1/215 (0%) Query: 33 AYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEVEL 92 AYAVQDA LGP+ GWKVG+ G P AP+ A V +G T+ R G+E E+ Sbjct: 39 AYAVQDAVGRGLGPIVGWKVGASGPEAEPAAAPIHAGTVFGTGATVPHGLCRHLGVEGEI 98 Query: 93 ALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALV 152 R R + AE+ I P IE+V+TR P + +AD QSHGAL+ Sbjct: 99 GYRFARALPARPRAYARAEVLAAIGTIHPVIEIVDTRFEKPASQHHLLHLADQQSHGALI 158 Query: 153 TGAPQALPDVLSLRQIEASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRAG 211 G Q ++ LSIDG+ V+ TGGN A D LL WLA H ARRG AG Sbjct: 159 VGPGQTAWQAVNPVAERVVLSIDGRVVADHTGGNAAGDPLRLLVWLANHAARRGIGIGAG 218 Query: 212 QIITTGSCTCHTMARGGRRVEAALHGIGAVWLDFA 246 ++TTGS T G EA IG V A Sbjct: 219 CLVTTGSATGTVFVAHGTDAEARFPNIGQVTAHLA 253