BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0244.p (446 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774150.1| tRNA modifying 2-methylthioadenine synthetas... 918 0.0 ref|YP_004754255.1| tRNA-i(6)A37 methylthiotransferase [Collimon... 787 0.0 ref|YP_001352121.1| tRNA modifying enzyme [Janthinobacterium sp.... 754 0.0 ref|YP_001098733.1| (dimethylallyl)adenosine tRNA methylthiotran... 753 0.0 ref|YP_004361962.1| 2-methylthioadenine synthetase [Burkholderia... 701 0.0 ref|YP_001856651.1| (dimethylallyl)adenosine tRNA methylthiotran... 696 0.0 gb|EGD02810.1| (dimethylallyl)adenosine tRNA methylthiotransfera... 696 0.0 ref|YP_002230065.1| (dimethylallyl)adenosine tRNA methylthiotran... 694 0.0 ref|YP_001809303.1| (dimethylallyl)adenosine tRNA methylthiotran... 693 0.0 ref|YP_774636.1| (dimethylallyl)adenosine tRNA methylthiotransfe... 693 0.0 >ref|YP_003774150.1| tRNA modifying 2-methylthioadenine synthetase [Herbaspirillum seropedicae SmR1] gb|ADJ62242.1| tRNA modifying 2-methylthioadenine synthetase protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 918 bits (2373), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/446 (100%), Positives = 446/446 (100%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF Sbjct: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY Sbjct: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH Sbjct: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQIIEVSITEAFPFSLRGEIVTQH Sbjct: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 >ref|YP_004754255.1| tRNA-i(6)A37 methylthiotransferase [Collimonas fungivorans Ter331] gb|AEK63432.1| tRNA-i(6)A37 methylthiotransferase [Collimonas fungivorans Ter331] Length = 450 Score = 787 bits (2032), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/443 (83%), Positives = 409/443 (92%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPE+ADVILLNTCS+REKAQEKVF Sbjct: 5 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEDADVILLNTCSVREKAQEKVF 64 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK+NNP LLIGVGGCVASQEG AI+KRAP+VDMVFGPQTLHRLP+MIS+RR Sbjct: 65 SDLGRLRELKRNNPDLLIGVGGCVASQEGAAIVKRAPYVDMVFGPQTLHRLPEMISQRRA 124 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +G QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 125 TGHSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 184 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGLA+QGVKEI LLGQNVNA+RG M DGEIADFALLIEYIAE+PGIERIRFVTSH Sbjct: 185 VLTEVAGLAEQGVKEITLLGQNVNAFRGAMADGEIADFALLIEYIAEIPGIERIRFVTSH 244 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR L+AMKRGYT LEYKSVIRR+R+VRP+I Sbjct: 245 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILSAMKRGYTVLEYKSVIRRLRQVRPNI 304 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 T+ SDFIVGFPGET+ DFEALMKL+ D+G+DNSFSFIFS RPGTPAANL DDTP EVKL Sbjct: 305 TISSDFIVGFPGETDADFEALMKLITDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLK 364 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQ LQA + N A+ISAA VG+VQR+LVEG SK+ ELQGRTE+NRVVNF+ GPNG R Sbjct: 365 RLQHLQATVEANMAKISAAMVGSVQRILVEGPSKKNPAELQGRTENNRVVNFNAGPNGAR 424 Query: 421 LIGQIIEVSITEAFPFSLRGEIV 443 LIG++I+V+ITE ++LRGEIV Sbjct: 425 LIGELIDVTITETLTYTLRGEIV 447 >ref|YP_001352121.1| tRNA modifying enzyme [Janthinobacterium sp. Marseille] sp|A6SV24.1|MIAB_JANMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ABR88458.1| tRNA modifying enzyme [Janthinobacterium sp. Marseille] Length = 446 Score = 754 bits (1947), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/446 (79%), Positives = 401/446 (89%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKM DVLNAS+GLIKTD PE+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK NNP L+IGVGGCVASQEG+AI+KRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VL EVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAEL GIERIRFVTSH Sbjct: 181 VLAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLID YAKVPKL NHLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R+VRP+I Sbjct: 241 PKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 ++ SDFIVGFPGET+ DFEA+MKL+ D+G+DNSFSFIFS RPGTPAANL DDTP EVKL Sbjct: 301 SISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQ 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N R S VGTVQR+LVEG SK+ ELQGRTE+NRVVNF G +G R Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGAR 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQ+++V+I ++F ++LRGEI+ + Sbjct: 421 LIGQMVDVNIVQSFAYTLRGEIIVKQ 446 >ref|YP_001098733.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Herminiimonas arsenicoxydans] sp|A4G267.1|MIAB_HERAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase emb|CAL60604.1| putative tRNA-i(6)A37 thiotransferase enzyme MiaB [Herminiimonas arsenicoxydans] Length = 446 Score = 753 bits (1943), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/446 (79%), Positives = 400/446 (89%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKMADVLNASDGLIKTD+ E+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMADVLNASDGLIKTDKAEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK P L+IGVGGCVASQEG+AIIKRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKLLKPDLMIGVGGCVASQEGDAIIKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRF+D Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAE+ GIERIR+VTSH Sbjct: 181 VLTEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAEMEGIERIRYVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R VRP+I Sbjct: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLRAVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 ++ SDFIVGFPGET+EDFEA+MKL+ D+G+DNSFSFIFS RPGTPAANL DDTP EVKL Sbjct: 301 SISSDFIVGFPGETDEDFEAMMKLIDDIGYDNSFSFIFSPRPGTPAANLADDTPHEVKLK 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N R S VG+VQR+LVEG SK+ ELQGRTE+NRVVNF GPN Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGSVQRILVEGPSKKDADELQGRTENNRVVNFSAGPNAAH 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQ++ V+IT++ ++LRGEIV + Sbjct: 421 LIGQMVNVNITQSHAYTLRGEIVVKQ 446 >ref|YP_004361962.1| 2-methylthioadenine synthetase [Burkholderia gladioli BSR3] gb|AEA62006.1| 2-methylthioadenine synthetase [Burkholderia gladioli BSR3] Length = 457 Score = 701 bits (1808), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/449 (73%), Positives = 385/449 (85%), Gaps = 3/449 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KK++IKTFGCQMNEYDSDKM DVLNAS+GL+KTDRPE+ADVIL NTCS+REKAQEKVF Sbjct: 1 MTKKIYIKTFGCQMNEYDSDKMVDVLNASEGLVKTDRPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ NP L++GVGGCVASQEG AI+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEANPNLIVGVGGCVASQEGAAIVARAPYVDVVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTE+AGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+AELPGIERIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGALTQGSEEIADFATLIEYVAELPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEFSQRL+D YA+VPKL NHL+LP QHGSDR L AMKRGYT LEYKS+IR++R +RP Sbjct: 241 SHPKEFSQRLLDVYARVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D I+GFPGETE DF+ M+LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDIIIGFPGETEADFDKTMQLVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARISA+ VG ++R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISASMVGRIERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIVTQH 446 RLIGQ+I+V I A+P SLRGE+V H Sbjct: 421 HARLIGQMIDVRINHAYPHSLRGELVLAH 449 >ref|YP_001856651.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Burkholderia phymatum STM815] sp|B2JD88.1|MIAB_BURP8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ACC69605.1| tRNA-i(6)A37 thiotransferase enzyme MiaB [Burkholderia phymatum STM815] Length = 456 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/449 (72%), Positives = 384/449 (85%), Gaps = 3/449 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVL A++GL+KTD PE+ADVIL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ NP LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEANPNLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGRPQVDI+FPEIEKFDHLPPAR++G +A+VSIMEGCSKYCSYCVVPYTRGEEVSR +D Sbjct: 121 SGRPQVDITFPEIEKFDHLPPARIDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTE+AGLADQGV+E+ LLGQNVNAYRG M G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGAMTLGATEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M+LV D+ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMQLVHDMSYDTSFSFIYSPRPGTPAANLQDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNG 418 L RLQ LQA + +N RIS A VG V+R+LVE +++ +EL GRTE+NRVVNF Sbjct: 361 LRRLQHLQATIEENVQRISQAMVGKVERILVERPARKDPNELAGRTENNRVVNFPAPVET 420 Query: 419 Q-RLIGQIIEVSITEAFPFSLRGEIVTQH 446 RLIGQ+I+V I A+P SLRGE+V H Sbjct: 421 HARLIGQMIDVKIVHAYPHSLRGELVMVH 449 >gb|EGD02810.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Burkholderia sp. TJI49] Length = 457 Score = 696 bits (1796), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/446 (73%), Positives = 382/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDI+FPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL NHL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV D+ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHDMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE+V Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELV 446 >ref|YP_002230065.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Burkholderia cenocepacia J2315] sp|B4EBG2.1|MIAB_BURCJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase emb|CAR51217.1| putative tRNA thiotransferase protein MiaB [Burkholderia cenocepacia J2315] Length = 457 Score = 694 bits (1790), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDI+FPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL NHL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETE+DF+ +M LV ++G+D SFSFI+S RPGTPAANL DDTP++VK Sbjct: 301 DLSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPRDVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE+V Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELV 446 >ref|YP_001809303.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Burkholderia ambifaria MC40-6] sp|B1YWB9.1|MIAB_BURA4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ACB65087.1| tRNA-i(6)A37 thiotransferase enzyme MiaB [Burkholderia ambifaria MC40-6] Length = 457 Score = 693 bits (1789), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV+IKTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYIKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE++ Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELL 446 >ref|YP_774636.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Burkholderia ambifaria AMMD] sp|Q0BC21.1|MIAB_BURCM RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ABI88302.1| tRNA-i(6)A37 thiotransferase enzyme MiaB [Burkholderia ambifaria AMMD] Length = 457 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE++ Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELL 446