BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0178.p (168 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774087.1| Type IV pilin A protein [Herbaspirillum sero... 331 2e-89 emb|CBJ39093.1| Type 4 fimbrial pilin signal peptide protein [Ra... 112 2e-23 ref|NP_518679.1| type 4 fimbrial pilin signal peptide protein [R... 105 3e-21 ref|NP_903879.1| type IV pilin [Chromobacterium violaceum ATCC 1... 105 3e-21 ref|ZP_03584319.1| putative type IV pilin protein PilA [Burkhold... 102 2e-20 ref|YP_002230113.1| putative type IV pilin protein [Burkholderia... 101 3e-20 ref|ZP_03573201.1| putative type IV pilin protein PilA [Burkhold... 100 7e-20 emb|CAQ56871.1| type 4 fimbrial pilin protein [Ralstonia solanac... 100 1e-19 ref|YP_001120583.1| general secretion pathway protein H [Burkhol... 100 1e-19 emb|CBX00209.1| Tfp pilus assembly protein, major type IV pilin ... 99 2e-19 >ref|YP_003774087.1| Type IV pilin A protein [Herbaspirillum seropedicae SmR1] gb|ADJ62179.1| Type IV pilin A protein [Herbaspirillum seropedicae SmR1] Length = 168 Score = 331 bits (848), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 167/168 (99%), Positives = 167/168 (99%) Query: 1 MKAAVTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVT 60 MKAAVTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVT Sbjct: 1 MKAAVTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVT 60 Query: 61 EQISSNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGGTLTLT 120 EQISSNNGVFTVAQLGLPAFTPTANVT ISVSPMQPRGVGGVITITYSAQVGEGGTLTLT Sbjct: 61 EQISSNNGVFTVAQLGLPAFTPTANVTGISVSPMQPRGVGGVITITYSAQVGEGGTLTLT 120 Query: 121 PTIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCRG 168 PTIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCRG Sbjct: 121 PTIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCRG 168 >emb|CBJ39093.1| Type 4 fimbrial pilin signal peptide protein [Ralstonia solanacearum CMR15] Length = 163 Score = 112 bits (280), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 69/173 (39%), Positives = 97/173 (56%), Gaps = 25/173 (14%) Query: 5 VTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQIS 64 + + Q+GFTL+ELM+ +AIVGILA + YQDY RARV+EG +LA K V E + Sbjct: 6 LNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAA 65 Query: 65 SNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGGTLTLTPT-- 122 + + G AF PT NV++++++ G I +T+++ V G TL + P+ Sbjct: 66 NAQSDLSA---GSSAFVPTKNVSNLTINSSN-----GTIQLTFTSAVSAGATLNMIPSSS 117 Query: 123 ---IASGT-----IQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 +A+G+ I W CAAAG T P TG AG+L AKYAPA CR Sbjct: 118 GVALATGSAPGAAIMWTCAAAGKTSIPV-------TGASAGSLAAKYAPAECR 163 >ref|NP_518679.1| type 4 fimbrial pilin signal peptide protein [Ralstonia solanacearum GMI1000] emb|CAD14088.1| type 4 fimbrial pilin signal peptide protein [Ralstonia solanacearum GMI1000] Length = 168 Score = 105 bits (261), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 73/176 (41%), Positives = 98/176 (55%), Gaps = 27/176 (15%) Query: 5 VTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQIS 64 + + Q+GFTL+ELM+ +AIVGILA + YQDY RARV+EG +LA K V+E + Sbjct: 7 LNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAA 66 Query: 65 SNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVG---GVITITYSAQVGEGGTLTLTP 121 + +V G FTPT NV +++++ G G G IT+TY+ G GGTL L P Sbjct: 67 NAQSDLSV---GSSVFTPTKNVANLTIA-----GTGTVPGQITVTYTTAAG-GGTLALVP 117 Query: 122 TIA----------SGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 T A SG I W C A QA + + A +G + +L AKYAPA CR Sbjct: 118 TAAGTALPVSSAPSGPIMWTCYAQNKAQAAS---SVAPSGTM--SLAAKYAPAECR 168 >ref|NP_903879.1| type IV pilin [Chromobacterium violaceum ATCC 12472] gb|AAQ61869.1| type IV pilin [Chromobacterium violaceum ATCC 12472] Length = 155 Score = 105 bits (261), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 67/162 (41%), Positives = 89/162 (54%), Gaps = 18/162 (11%) Query: 10 QQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQISSNNGV 69 QQGFTL+ELM+ +AIVGILA + YQDY RARVSEG LA+ K AVTE ++NN Sbjct: 7 QQGFTLIELMIVVAIVGILAAIAIPAYQDYTKRARVSEGLALADAAKTAVTEYYATNNAW 66 Query: 70 FTVAQLGLPAFTPT----ANVTSISVSPMQPRGVGGVITITYSAQVGEGGTLTLTPTIAS 125 + G F T A++T SVS + GV+ I Y++ V G + L PT+++ Sbjct: 67 AS----GTAPFNTTYGLAASITGNSVSSINVLA-NGVVQIAYNSTVSNGALVDLVPTVSA 121 Query: 126 GTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 G++QW C G G TA A L A + P+ CR Sbjct: 122 GSVQWYCTYTG-------SGTTAIAS--ASQLSANWVPSTCR 154 >ref|ZP_03584319.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD1] gb|EEE01169.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD1] Length = 199 Score = 102 bits (255), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 70/186 (37%), Positives = 92/186 (49%), Gaps = 29/186 (15%) Query: 2 KAAVTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTE 61 + A R+ + GFTL+ELM+ LAIVG++A YQDY+AR+RV EG LA +LAV + Sbjct: 22 RPAPLRAWRAGFTLIELMIVLAIVGVVAAYAIPAYQDYLARSRVGEGLALASSARLAVAD 81 Query: 62 QISSNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGG--TLTL 119 +S G+ G A T NV SI + G IT+ Y+A+V G TL L Sbjct: 82 NAASGTGL----DGGYSAPAATRNVESIRID-----AETGQITVAYTARVAAAGANTLVL 132 Query: 120 TPT------------------IASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKY 161 P+ + +G I W+C AAG + P A TLPAKY Sbjct: 133 VPSAPDKPDTPTARVPLKKGEMQAGAIAWECFAAGKDASSLPAPGAGPLPGTAATLPAKY 192 Query: 162 APANCR 167 APA CR Sbjct: 193 APAECR 198 >ref|YP_002230113.1| putative type IV pilin protein [Burkholderia cenocepacia J2315] emb|CAR51266.1| putative type IV pilin protein [Burkholderia cenocepacia J2315] Length = 198 Score = 101 bits (252), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 70/181 (38%), Positives = 90/181 (49%), Gaps = 29/181 (16%) Query: 7 RSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQISSN 66 R GFTL+ELM+ LAIVG++A YQDY+AR+RV EG LA +LAV + +S Sbjct: 26 RWRPAGFTLIELMIVLAIVGVVAAYAIPAYQDYLARSRVGEGLALASSARLAVADNAASG 85 Query: 67 NGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGGTLTL------- 119 + G T NV S+++ GV G IT+ ++ +V GT TL Sbjct: 86 TSL----DGGYSPPAATRNVESVAID-----GVTGQITVAFTTRVAAAGTNTLVLVPSAP 136 Query: 120 ----TP---------TIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANC 166 TP TI +GTI W+C AAG + P A TLPAKYAPA C Sbjct: 137 DKADTPTARVPLKKGTIQAGTIAWECFAAGKDASSLPAPGAGPLPSNAATLPAKYAPAEC 196 Query: 167 R 167 R Sbjct: 197 R 197 >ref|ZP_03573201.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD2M] ref|ZP_03579701.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD2] gb|EEE05779.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD2] gb|EEE12801.1| putative type IV pilin protein PilA [Burkholderia multivorans CGD2M] Length = 199 Score = 100 bits (249), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 68/178 (38%), Positives = 88/178 (49%), Gaps = 29/178 (16%) Query: 10 QQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQISSNNGV 69 + GFTL+ELM+ LAIVG++A YQDY+AR+RV EG LA +LAV + +S G+ Sbjct: 30 RAGFTLIELMIVLAIVGVVAAYAIPAYQDYLARSRVGEGLALASSARLAVADNAASGTGL 89 Query: 70 FTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGG--TLTLTPT----- 122 G A T NV SI + G IT+ Y+A+V G TL L P+ Sbjct: 90 ----DGGYSAPAATRNVESIRIDAET-----GQITVAYTARVAAAGANTLVLVPSAPDKP 140 Query: 123 -------------IASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 + +G I W+C AAG + P A TLPAKYAPA CR Sbjct: 141 DTPTARVPLKKGEMQAGAIAWECFAAGKDASSLPAPGAGPLPGTAATLPAKYAPAECR 198 >emb|CAQ56871.1| type 4 fimbrial pilin protein [Ralstonia solanacearum MolK2] Length = 168 Score = 100 bits (248), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 71/176 (40%), Positives = 93/176 (52%), Gaps = 27/176 (15%) Query: 5 VTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQIS 64 + + Q+GFTL+ELM+ +AIVGILA + YQDY RARV+EG +LA K V+E + Sbjct: 7 LNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAA 66 Query: 65 SNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGV---ITITYSAQVGEGGTLTLTP 121 + +V G FT T NV +++++ G GGV IT+TY+ G GGTL L P Sbjct: 67 NAQSDLSV---GSSVFTATKNVANLTIA-----GTGGVPGQITVTYTTAAG-GGTLALVP 117 Query: 122 TIA----------SGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 T A SG I W C A A + + TL AKYAPA CR Sbjct: 118 TAAGSALPISSAPSGPIMWTCYAQNKAAAASSVSPSGTM-----TLAAKYAPAECR 168 >ref|YP_001120583.1| general secretion pathway protein H [Burkholderia vietnamiensis G4] gb|ABO55748.1| general secretion pathway protein H [Burkholderia vietnamiensis G4] Length = 198 Score = 99.8 bits (247), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/183 (37%), Positives = 88/183 (48%), Gaps = 29/183 (15%) Query: 5 VTRSSQQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQIS 64 V R GFTL+ELM+ LAIVG++A YQDY+AR+RV EG LA +LAV + + Sbjct: 24 VPRWHPAGFTLIELMIVLAIVGVIAAYAIPAYQDYLARSRVGEGLALASSARLAVADNAA 83 Query: 65 SNNGVFTVAQLGLPAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGG--TLTLTP- 121 S + G A T NV S+ + G G IT+ ++A+V G TL L P Sbjct: 84 SGAAL----DGGYSAPAATRNVESVRID-----GDTGQITVAFTARVAAAGANTLVLVPS 134 Query: 122 -----------------TIASGTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPA 164 T+ +G I W+C A G + P A TLPAKYAPA Sbjct: 135 APDKADAPTARVPLKKGTMQAGAIAWECFAGGKDASSLPAPGAGPMPAEAATLPAKYAPA 194 Query: 165 NCR 167 CR Sbjct: 195 ECR 197 >emb|CBX00209.1| Tfp pilus assembly protein, major type IV pilin class A [Legionella pneumophila 130b] Length = 140 Score = 99.0 bits (245), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 75/162 (46%), Positives = 95/162 (58%), Gaps = 33/162 (20%) Query: 10 QQGFTLLELMVTLAIVGILAMLGASQYQDYIARARVSEGFNLAEPYKLAVTEQISSNNGV 69 Q+GFTL+ELM+ +AI+GILA + YQDY RARV+EG LA+ KLAV+E +NN + Sbjct: 7 QKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTLADSAKLAVSETAITNNAL 66 Query: 70 -FTVAQLGL--PAFTPTANVTSISVSPMQPRGVGGVITITYSAQVGEGGTLTLTPTI-AS 125 T A G PA TP NV SI++ G GVITITY+A G GGT+ +TPT+ A+ Sbjct: 67 PATQAATGYVSPAATP--NVQSIAI------GANGVITITYTAAAG-GGTIIMTPTLQAN 117 Query: 126 GTIQWQCAAAGVTQAPTPQGATANTGFVAGTLPAKYAPANCR 167 G + W C AGTL AKY PA+CR Sbjct: 118 GDVTWTC--------------------TAGTLLAKYRPASCR 139