BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0154.p (438 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004152589.1| major facilitator superfamily protein [Vario... 409 e-112 ref|YP_003452131.1| proline/betaine transporter [Azospirillum sp... 407 e-111 ref|YP_297491.1| major facilitator transporter [Ralstonia eutrop... 405 e-110 ref|YP_300000.1| major facilitator transporter [Ralstonia eutrop... 390 e-106 ref|YP_002981410.1| major facilitator superfamily protein [Ralst... 387 e-105 ref|YP_001898982.1| major facilitator superfamily protein [Ralst... 385 e-104 ref|YP_586640.1| General substrate transporter:Major facilitator... 382 e-104 ref|YP_587546.1| major facilitator superfamily permease [Cupriav... 382 e-104 ref|YP_001895794.1| major facilitator superfamily protein [Burkh... 370 e-100 ref|YP_336818.1| major facilitator family transporter [Burkholde... 368 1e-99 >ref|YP_004152589.1| major facilitator superfamily protein [Variovorax paradoxus EPS] gb|ADU34478.1| major facilitator superfamily MFS_1 [Variovorax paradoxus EPS] Length = 449 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 208/434 (47%), Positives = 292/434 (67%), Gaps = 2/434 (0%) Query: 3 PSSAPAFSDADA--ADKVVRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPA 60 P++A S AD A + + +FR+IAA ++GNALEM+DFT++SFFA++IGK FFP+ Sbjct: 16 PTAAAPSSPADTVQAKGKHKGKQTQFRLIAACSLGNALEMYDFTVYSFFALLIGKLFFPS 75 Query: 61 TTPLASLLMTMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPS 120 +P SLL+ +ATFG GFVMRPLGG+V+G+YADR GRK A+ L LM AGT I + P+ Sbjct: 76 DSPYGSLLLAVATFGIGFVMRPLGGVVIGSYADRKGRKAALTLTIGLMVAGTLCIALAPT 135 Query: 121 YDSIGVLAPLLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALV 180 Y S GV L+I+ GRLLQGFS+GGE+GA+T++L E+ RGF +SWQ+ASQG AA++ Sbjct: 136 YASAGVFGSLMILAGRLLQGFSLGGEIGAATSMLMESGGIRGRGFRVSWQLASQGIAAVL 195 Query: 181 GALTGYLLSTFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLV 240 GALTG L L Q L+SWGWRLPFLFGL++ P+G+ IR+ L ET + + + Sbjct: 196 GALTGAALYAALPQASLESWGWRLPFLFGLLIAPVGFYIRANLEETHQGGRHESSPMGRL 255 Query: 241 FSRFYHPLITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSP 300 ++ G+ +T GTA+M++VVF+MPTY++ LHMP S+S L C G + + Sbjct: 256 LREHGGTVLKGIFSITAGTAAMYLVVFFMPTYMIRVLHMPPSLSLLSGCATGITMFAVAL 315 Query: 301 FAGLLSDHLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGA 360 AG L+D L RKP VI S +LG +YPAFWL+NH L L + LG+ Sbjct: 316 LAGRLADRLPNRKPLVIGSLVLGVLAVYPAFWLINHYPSVPLVLCLSVLLTAIVNLGTTP 375 Query: 361 SFLLLVESFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIV 420 FL+L+E P VRA+G S++YS+GV++FGG + F+VTWL+ +G+PMSPA YM+ C ++ Sbjct: 376 LFLILLEMLPIGVRASGISVIYSVGVTVFGGSSQFIVTWLLATTGNPMSPAFYMMACGVL 435 Query: 421 SLIALIPMKEHSTE 434 S+ AL+ ++ + Sbjct: 436 SICALVSLRARRAD 449 >ref|YP_003452131.1| proline/betaine transporter [Azospirillum sp. B510] dbj|BAI75587.1| proline/betaine transporter [Azospirillum sp. B510] Length = 446 Score = 407 bits (1046), Expect = e-111, Method: Compositional matrix adjust. Identities = 210/408 (51%), Positives = 289/408 (70%), Gaps = 2/408 (0%) Query: 29 IAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMRPLGGLVM 88 I A+++GNA+E++DFTI+SFF++ IGK FFP T LL+ + TFG GFV+RPLGG+++ Sbjct: 19 ILALSIGNAVELYDFTIYSFFSLTIGKLFFPVQTAYGPLLLALLTFGVGFVVRPLGGIII 78 Query: 89 GAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGFSMGGEVG 148 GAYADR GRKPA++L SLMA GT +IG+ P+Y IGV AP+ IV GR+LQGFS GGE+G Sbjct: 79 GAYADRAGRKPALVLTISLMALGTLLIGLCPTYAQIGVAAPVAIVIGRVLQGFSAGGEIG 138 Query: 149 ASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWGWRLPFLF 208 +T +L E RG +SWQM SQGAAAL+GAL+GYLLST L + L++WGWRLPFL Sbjct: 139 VATTVLMEMGSPARRGLRVSWQMVSQGAAALLGALSGYLLSTHLPAEALEAWGWRLPFLA 198 Query: 209 GLMLGPLGYAIRSMLHETPPIESRKGHAV-RLVFSRFYHPLITGVLLMTGGTASMFIVVF 267 GL++ P+G +R +L ET + R G AV + S L GVL++ GGTAS ++ ++ Sbjct: 199 GLLILPVGLYLRRLLPETARPD-RSGRAVLAELLSDHRSRLAAGVLMIAGGTASTYVNIY 257 Query: 268 YMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFSRLLGASLI 327 YMPTYL+ + +P+ +FL C+AG +++ +P AGLLSD + RR+PFV +S L+ L Sbjct: 258 YMPTYLIRIVGLPSRTAFLTGCVAGVTMVLVAPLAGLLSDRIDRRRPFVAWSALISMCLT 317 Query: 328 YPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFSLVYSIGVS 387 YPAF +L+H AL G L +G+G+ LLL+E+FP VRA+G S+VYS GV+ Sbjct: 318 YPAFRVLSHGPGVPAALAVAGLLIAFNAIGAGSCLLLLMENFPARVRASGLSIVYSAGVA 377 Query: 388 IFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHSTEP 435 IFGGFA F+VTWLI ++GDP+SPA Y+ C++ +L+A M+E + P Sbjct: 378 IFGGFAQFIVTWLISITGDPISPAWYVSACALATLLACTRMREVRSLP 425 >ref|YP_297491.1| major facilitator transporter [Ralstonia eutropha JMP134] gb|AAZ62647.1| General substrate transporter:Major facilitator superfamily MFS_1 [Ralstonia eutropha JMP134] Length = 431 Score = 405 bits (1040), Expect = e-110, Method: Compositional matrix adjust. Identities = 201/411 (48%), Positives = 282/411 (68%) Query: 21 KSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVM 80 + +++FR+IAA ++GNALEMFDFT++SFF+++IGK FFP+ P SLL+ +ATFG GFVM Sbjct: 18 RPQSQFRLIAACSLGNALEMFDFTVYSFFSLLIGKLFFPSDNPYGSLLLAVATFGIGFVM 77 Query: 81 RPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQG 140 RPLGG+V+G+YADR GRK AM L LM AGT I + P Y S GV L+I+ GRLLQG Sbjct: 78 RPLGGVVIGSYADRRGRKAAMTLTIGLMVAGTLCIALAPPYASAGVFGSLMILAGRLLQG 137 Query: 141 FSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSW 200 FS+GGE+GASTA+L E+ RG+ +SWQ+ SQG +A++GALTG L L + L+SW Sbjct: 138 FSLGGEIGASTAMLMESGGVRGRGWRVSWQLGSQGISAMLGALTGASLYALLPTESLESW 197 Query: 201 GWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFSRFYHPLITGVLLMTGGTA 260 GWRLPFL GL++ P+G IR+ L ET + + +F + ++ G+L T GTA Sbjct: 198 GWRLPFLVGLLIAPVGLYIRANLDETHQADEHAPSPLGELFRNHWATVVKGILATTAGTA 257 Query: 261 SMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFSR 320 +M++VVF+MPTY++ L MP S+SFL C+ G L V S AG L+D L RKP V+ S Sbjct: 258 TMYLVVFFMPTYMIRVLKMPPSLSFLSGCVTGLTLTVVSLVAGRLADRLTNRKPLVVASL 317 Query: 321 LLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFSL 380 + +YPAFWL+N L L + LG+ FL+++E P VRA+G S+ Sbjct: 318 VFSMLAVYPAFWLINAHPSVPLVLCLSALLTAGINLGTTPMFLMMLEMLPAGVRASGLSV 377 Query: 381 VYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEH 431 +YS+GV++FGG + F+VTWL+ +G+P+SPA YM+VC ++LIA++ ++E Sbjct: 378 IYSVGVTVFGGSSQFIVTWLLSRTGNPLSPAWYMLVCGAITLIAVLSIRER 428 >ref|YP_300000.1| major facilitator transporter [Ralstonia eutropha JMP134] gb|AAZ65156.1| General substrate transporter:Major facilitator superfamily MFS_1 [Ralstonia eutropha JMP134] Length = 440 Score = 390 bits (1001), Expect = e-106, Method: Compositional matrix adjust. Identities = 211/415 (50%), Positives = 284/415 (68%), Gaps = 7/415 (1%) Query: 3 PSSAPAFSDADAADKVVRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATT 62 P S A +DA + + R I A ++GNALE+FDFT+FSFFA +IGK FFP Sbjct: 15 PGSPTARADATTFAR-------QRRAIVAASLGNALELFDFTVFSFFAGLIGKLFFPVEG 67 Query: 63 PLASLLMTMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYD 122 LL+ +ATFG GFVMRP+G +V+G YADR GRKPA+ L LMA GT IIG+ P+Y Sbjct: 68 EYGPLLLALATFGIGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAIIGLAPTYA 127 Query: 123 SIGVLAPLLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGA 182 IG+ APLL+VTGRLLQGFS GGE+GA+T+ L E+ R++RGFM+SWQMA QGA+AL GA Sbjct: 128 QIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLMESGTRSNRGFMVSWQMAGQGASALAGA 187 Query: 183 LTGYLLSTFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFS 242 L G LL L Q L+SWGWR+PFL GL++GP+G+ IR L ET +R+ A+ Sbjct: 188 LCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERSALSEAVQ 247 Query: 243 RFYHPLITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFA 302 + L+ G LL+ GGTA M++VVF+ P +LV HMP + S L C AGAI+L+ SP A Sbjct: 248 QHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIMLLVSPLA 307 Query: 303 GLLSDHLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASF 362 G+L+D L RRKP ++ R++ I P F L+ + ALV + + TLG+ A F Sbjct: 308 GVLADRLGRRKPLLLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLATLGAPAGF 367 Query: 363 LLLVESFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVC 417 L ++ESFP+ VRATG +++Y+ GV++FGGFA F+VTW++ V+G+P+SPA Y++ C Sbjct: 368 LFIMESFPRRVRATGLAIIYACGVTLFGGFAQFIVTWMLSVTGNPLSPAWYVLGC 422 >ref|YP_002981410.1| major facilitator superfamily protein [Ralstonia pickettii 12D] ref|ZP_07676760.1| citrate MFS transporter [Ralstonia sp. 5_7_47FAA] gb|ACS62738.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12D] gb|EFP64763.1| citrate MFS transporter [Ralstonia sp. 5_7_47FAA] Length = 434 Score = 387 bits (993), Expect = e-105, Method: Compositional matrix adjust. Identities = 200/415 (48%), Positives = 277/415 (66%), Gaps = 1/415 (0%) Query: 21 KSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVM 80 K + R+IAA ++GNALEM+DFT++SFFA+MIGK FFP+ + SLL+ +ATFG GFVM Sbjct: 20 KPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSDSAYGSLLLAVATFGIGFVM 79 Query: 81 RPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQG 140 RPLGG V+G YADR GRK AM L LM GT + V P+Y + G+L PL+I+ GRLLQG Sbjct: 80 RPLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTYATAGLLGPLVILAGRLLQG 139 Query: 141 FSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSW 200 FS+GGE+GASTA+L E+ RGF +SWQ+ SQG AAL GALT +L LS + L SW Sbjct: 140 FSLGGEIGASTAMLMESGGIRGRGFRVSWQLGSQGIAALCGALTAAILYGSLSPEALQSW 199 Query: 201 GWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFSRFYHPLITGVLLMTGGTA 260 GWR+PF GL++ P+G IRS L ET E+ + +F +I GVL + GGT Sbjct: 200 GWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLFRDHGGLVIKGVLTIIGGTV 259 Query: 261 SMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFSR 320 ++V+++MPTY++ LH+P S+S L C+ G S +G L+D L RRKP V+ + Sbjct: 260 GTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLLSGWLADRLPRRKPLVVGAT 319 Query: 321 LLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFSL 380 L+ + +YPAFWL+ H L L G L LG+ L+++E P VRA+G S+ Sbjct: 320 LVTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPMLLMMMELLPIKVRASGLSV 379 Query: 381 VYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKE-HSTE 434 +Y+IGV++FGG FVVTWL+ +G+P+SPA YM+ CS ++L+ ++ ++E H T+ Sbjct: 380 IYAIGVTVFGGSCQFVVTWLLARTGNPLSPAFYMMACSAITLLTVLSLREAHETD 434 >ref|YP_001898982.1| major facilitator superfamily protein [Ralstonia pickettii 12J] gb|ACD26550.1| major facilitator superfamily MFS_1 [Ralstonia pickettii 12J] Length = 434 Score = 385 bits (988), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/410 (48%), Positives = 274/410 (66%) Query: 21 KSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVM 80 K + R+IAA ++GNALEM+DFT++SFFA+MIGK FFP+ + SLL+ +ATFG GFVM Sbjct: 20 KPAGQARLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSDSAYGSLLLAVATFGIGFVM 79 Query: 81 RPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQG 140 RPLGG V+G YADR GRK AM L LM GT + V P+Y + G+L PL+I+ GRLLQG Sbjct: 80 RPLGGFVIGNYADRHGRKAAMTLTIGLMVIGTLCLAVAPTYATAGLLGPLVILAGRLLQG 139 Query: 141 FSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSW 200 FS+GGE+GASTA+L E+ RGF +SWQ+ SQG AAL GALT +L LS + L SW Sbjct: 140 FSLGGEIGASTAMLMESGGIRGRGFRVSWQLGSQGIAALCGALTAAILYGSLSPEALQSW 199 Query: 201 GWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFSRFYHPLITGVLLMTGGTA 260 GWR+PF GL++ P+G IRS L ET E+ + +F +I GVL + GGT Sbjct: 200 GWRVPFFLGLLIAPVGLYIRSHLDETHTAEAHAPSPLGTLFRDHGGLVIKGVLTIIGGTV 259 Query: 261 SMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFSR 320 ++V+++MPTY++ LH+P S+S L C+ G S +G L+D L RRKP V+ + Sbjct: 260 GTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFGASLLSGWLADRLPRRKPLVVGAT 319 Query: 321 LLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFSL 380 L+ + +YPAFWL+ H L L G L LG+ L+++E P VRA+G S+ Sbjct: 320 LVTVAALYPAFWLMTHYPSVPLVLALSALLTGTLYLGTTPMLLMMMELLPIKVRASGLSV 379 Query: 381 VYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKE 430 +Y+IGV++FGG FVVTWL+ +G+P+SPA YM+ CS ++L+ ++ ++E Sbjct: 380 IYAIGVTVFGGSCQFVVTWLLARTGNPLSPAFYMMACSAITLLTVLSLRE 429 >ref|YP_586640.1| General substrate transporter:Major facilitator superfamily MFS_1 [Cupriavidus metallidurans CH34] gb|ABF11371.1| General substrate transporter:Major facilitator superfamily MFS_1 [Cupriavidus metallidurans CH34] Length = 434 Score = 382 bits (982), Expect = e-104, Method: Compositional matrix adjust. Identities = 200/411 (48%), Positives = 272/411 (66%), Gaps = 2/411 (0%) Query: 20 RKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFV 79 R S+A R+IAA ++GNALEM+DFT++SFFA+MIGK FFP+ + SLL+ +ATFG GFV Sbjct: 21 RTSQA--RLIAACSIGNALEMYDFTVYSFFALMIGKLFFPSESAYGSLLLAVATFGIGFV 78 Query: 80 MRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQ 139 MRPLGG V+G YADR GRK AM L LM GT + V P+Y + G+L PL+I+ GRLLQ Sbjct: 79 MRPLGGFVIGNYADRHGRKAAMTLTIGLMVVGTLCLAVAPTYATAGLLGPLIILAGRLLQ 138 Query: 140 GFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDS 199 GFS+GGE+GASTA+L EA RGF +SWQ+ SQG AAL GALT +L LS + L S Sbjct: 139 GFSLGGEIGASTAMLMEAGGIRGRGFRVSWQLGSQGIAALCGALTAAVLYGSLSTEALQS 198 Query: 200 WGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFSRFYHPLITGVLLMTGGT 259 WGWR+PF GL++ P+G+ IRS L ET E+ + +F + GVL + GGT Sbjct: 199 WGWRVPFFLGLLIAPVGFYIRSHLDETHTAETHAPSPLGTLFREHGTLVAKGVLTIIGGT 258 Query: 260 ASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFS 319 ++V+++MPTY++ LH+P S+S L C+ G S +G L+D L RRKP V+ S Sbjct: 259 VGTYLVIYFMPTYMIRELHLPASLSLLAGCVTGLTSFAASLCSGWLADRLQRRKPLVVAS 318 Query: 320 RLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFS 379 L +YPAFWL+ H L L L LG+ L+++E P VRA+G S Sbjct: 319 ILSTVVALYPAFWLMTHYPSVPLVLSLSALLTASLYLGTTPLLLMMMEMLPIKVRASGLS 378 Query: 380 LVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKE 430 ++Y+IGV++FGG FVVTWL+ +G+ +SPA YM+ CS V+L+ ++ ++E Sbjct: 379 VIYAIGVTVFGGSCQFVVTWLLARTGNQLSPAFYMMACSAVTLLTVLTLRE 429 >ref|YP_587546.1| major facilitator superfamily permease [Cupriavidus metallidurans CH34] gb|ABF12277.1| Permease of the major facilitator superfamily (MFS); putative citrate transporter [Cupriavidus metallidurans CH34] Length = 434 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 216/422 (51%), Positives = 282/422 (66%), Gaps = 3/422 (0%) Query: 12 ADAADKVVR--KSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLM 69 +DAA +VV S R + A +GN LE++DFT++SFFA IG+ FFPA++PLASLL+ Sbjct: 10 SDAAARVVAPASSTQGRRAVIAAAIGNGLEIYDFTVYSFFAATIGRLFFPASSPLASLLL 69 Query: 70 TMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAP 129 + ATFGAGFVMRPLG +++G ADR GRK A+ L LM AGT ++ TPSY SIGV A Sbjct: 70 SFATFGAGFVMRPLGAVLIGNMADRRGRKAALTLTIGLMTAGTALLAFTPSYASIGVTAT 129 Query: 130 LLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLS 189 L+V GRLLQG S GGEVGA++ALL E+ + R F +SWQ ASQGAAAL+GALTG +S Sbjct: 130 ALVVVGRLLQGLSAGGEVGAASALLMESTGKQRRCFAVSWQGASQGAAALLGALTGAAVS 189 Query: 190 TFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRLVFSRFYHPLI 249 LS + L SWGWR+PF+ GL++ P+G IR L ET E+ + V LI Sbjct: 190 ALLSPEALQSWGWRVPFMLGLLIAPVGIYIRRHLEET-QTENTAHAGLMTVVREHRRTLI 248 Query: 250 TGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHL 309 GVLLMT T+SM+I+VFYMPTYLV +HMP +FLGAC+AG +LV SP GLL+D L Sbjct: 249 LGVLLMTSSTSSMYIMVFYMPTYLVNTVHMPPVTAFLGACVAGLTMLVMSPLFGLLADRL 308 Query: 310 ARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESF 369 RRKP +I + ++ S++ P F LL+ L I ++G GA L++ES Sbjct: 309 PRRKPLLIANSIVAISVMVPNFALLSSGPGLPLTLACIALSVTLSSVGGGAGSALMMESL 368 Query: 370 PQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMK 429 P+ RATG S++YS+GV++FGGFAP VVTWLI +G M+P YM+ S++S AL+ Sbjct: 369 PRHHRATGMSIMYSVGVTVFGGFAPLVVTWLIATTGSRMAPVGYMLCASLISFTALLLFP 428 Query: 430 EH 431 H Sbjct: 429 SH 430 >ref|YP_001895794.1| major facilitator superfamily protein [Burkholderia phytofirmans PsJN] gb|ACD16570.1| major facilitator superfamily MFS_1 [Burkholderia phytofirmans PsJN] Length = 440 Score = 370 bits (950), Expect = e-100, Method: Compositional matrix adjust. Identities = 204/441 (46%), Positives = 285/441 (64%), Gaps = 11/441 (2%) Query: 5 SAPAFSDA--DAADKVVRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATT 62 +P+ S + D + R + + +I A T+GNA E FD T++SFFAV++GK FFP + Sbjct: 2 KSPSLSSSLHDVSHGTTRGASTR-GLIVATTIGNAFEFFDLTVYSFFAVLLGKLFFPLAS 60 Query: 63 PLASLLMTMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYD 122 LL+++ TFG GFV RPLGG++ G ADRFGR AM L MA GTG+IGV P+Y Sbjct: 61 SQQQLLLSVGTFGIGFVARPLGGVLFGVLADRFGRIAAMNLTLVTMAVGTGLIGVMPTYA 120 Query: 123 SIGVLAPLLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGA 182 IG+ APL IV RLLQGFS GGEVG +T LLAE A RGF+ SWQ ASQGAAAL G+ Sbjct: 121 QIGIAAPLAIVAARLLQGFSAGGEVGVATTLLAERASTGRRGFITSWQFASQGAAALAGS 180 Query: 183 LTGYLLSTFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSML------HETPPIESRKGHA 236 L G+LLS +L + L++WGWR+PFL G+++ PLG +RS L + P +R Sbjct: 181 LVGFLLSHYLDKASLEAWGWRIPFLAGILIAPLGLYLRSRLGAEGDKSDAPADAARDSAG 240 Query: 237 V-RLVFSRFYHPLITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAIL 295 V + V +R + ++ LL+ GGT++ FIV++YM TY + LH+P V+ LAGA++ Sbjct: 241 VLKTVMTRDWRLVLVAFLLVIGGTSAHFIVIYYMSTYAIKVLHLPMDVALWCGILAGAVM 300 Query: 296 LVGSPFAGLLSDHLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLT 355 LV SP G+ SD R++ I SRLL LI P F+L+ H + + + LA + Sbjct: 301 LVMSPVGGIWSDMYGRKRVTFI-SRLLLTLLILPGFYLIEHAPSVSTLYLVVCGLAALHS 359 Query: 356 LGSGASFLLLVESFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMI 415 + +GA+ LL E FP++ RATG S+ Y+ GVSIFGGFA F+VTWLI+ +G+P +PA Y+I Sbjct: 360 INAGANGTLLPEMFPRSTRATGLSIAYAAGVSIFGGFAQFIVTWLIQSTGNPAAPAWYVI 419 Query: 416 VCSIVSLIALIPMKEHSTEPL 436 C ++SLIAL+ + + + + + Sbjct: 420 ACGVLSLIALLFVVDRTRQDI 440 >ref|YP_336818.1| major facilitator family transporter [Burkholderia pseudomallei 1710b] ref|ZP_03794250.1| major facilitator family transporter [Burkholderia pseudomallei Pakistan 9] ref|ZP_04889441.1| major facilitator family transporter [Burkholderia pseudomallei 1655] ref|ZP_04954745.1| major facilitator family transporter [Burkholderia pseudomallei 1710a] gb|ABA51203.1| major facilitator family transporter [Burkholderia pseudomallei 1710b] gb|EDU10425.1| major facilitator family transporter [Burkholderia pseudomallei 1655] gb|EEH25149.1| major facilitator family transporter [Burkholderia pseudomallei Pakistan 9] gb|EET04267.1| major facilitator family transporter [Burkholderia pseudomallei 1710a] Length = 437 Score = 368 bits (944), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 207/417 (49%), Positives = 269/417 (64%), Gaps = 6/417 (1%) Query: 25 KFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMRPLG 84 K R+IAA VGNALE +DFT++SFFA++IGK FFP +P L++ +A+FG GFV RPLG Sbjct: 21 KRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG 80 Query: 85 GLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGFSMG 144 GLV+G YADR GRK AM+ +MA GT I V P+Y IG+ APLL+V RLLQGF+ G Sbjct: 81 GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASG 140 Query: 145 GEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWGWRL 204 GEVGAST LL E A + RGF S+Q +SQG AAL GALTG LL++ LS +L+SWGWR+ Sbjct: 141 GEVGASTTLLIEQAPQARRGFYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRV 200 Query: 205 PFLFGLMLGPLGYAIRSMLHETP-PIESRKGHAVRL----VFSRFYHPLITGVLLMTGGT 259 PF+ G + PLGY +R + E P P + +G A L V + G+ + GGT Sbjct: 201 PFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPLPLADVLRHHGKAVFAGLGITIGGT 260 Query: 260 ASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIFS 319 + +I+VFYM Y V LH+P+ +S C+AGAIL V +P G LSD R + V ++ Sbjct: 261 SIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNR-IVWWT 319 Query: 320 RLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVESFPQAVRATGFS 379 R+ IYPAF LN A L I LA + GA+ +L E FP+AVRATG + Sbjct: 320 RVALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGATGAMLGELFPRAVRATGGA 379 Query: 380 LVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHSTEPL 436 LVYS+GV+IFGGFA F VTWLI +G+ +PA Y I C ++L AL M + + +PL Sbjct: 380 LVYSVGVAIFGGFAQFFVTWLIAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPL 436