BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0031.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum s... 702 0.0 ref|YP_001890410.1| 2-nitropropane dioxygenase NPD [Burkholderia... 382 e-104 ref|YP_004475193.1| 2-nitropropane dioxygenase [Pseudomonas fulv... 372 e-101 ref|YP_001579734.1| 2-nitropropane dioxygenase NPD [Burkholderia... 370 e-100 ref|ZP_03545659.1| 2-nitropropane dioxygenase NPD [Comamonas tes... 368 e-100 ref|YP_004485679.1| 2-nitropropane dioxygenase NPD [Delftia sp. ... 368 e-100 ref|YP_003276254.1| 2-nitropropane dioxygenase [Comamonas testos... 368 e-99 ref|YP_001561310.1| 2-nitropropane dioxygenase NPD [Delftia acid... 364 9e-99 ref|ZP_07047166.1| 2-nitropropane dioxygenase, NPD [Comamonas te... 363 2e-98 ref|YP_299320.1| 2-nitropropane dioxygenase [Ralstonia eutropha ... 358 6e-97 >ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] gb|ADJ64925.1| 2-nitropropane dioxygenase protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 702 bits (1812), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/356 (98%), Positives = 352/356 (98%) Query: 1 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL 60 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL Sbjct: 1 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL 60 Query: 61 QALTEKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQM 120 ALTEKPYN+NVFCHQPAVLDSERDRAWL+HLQPLLDEFGGRIAAPLKEIYQSFN DTQM Sbjct: 61 HALTEKPYNINVFCHQPAVLDSERDRAWLAHLQPLLDEFGGRIAAPLKEIYQSFNADTQM 120 Query: 121 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF 180 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF Sbjct: 121 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF 180 Query: 181 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMG 240 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDG GIAAALQLGAQAVQMG Sbjct: 181 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMG 240 Query: 241 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY 300 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY Sbjct: 241 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY 300 Query: 301 SAAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 AAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA Sbjct: 301 PAAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 >ref|YP_001890410.1| 2-nitropropane dioxygenase NPD [Burkholderia phytofirmans PsJN] gb|ACD21039.1| 2-nitropropane dioxygenase NPD [Burkholderia phytofirmans PsJN] Length = 367 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 200/330 (60%), Positives = 238/330 (72%) Query: 1 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL 60 M + T +Q +G HPIIQAPMAG ST +AAA SNAGALGS+A+GAS A+QARE + Sbjct: 1 MTSNTRFLQAIGAQHPIIQAPMAGISTPALAAAVSNAGALGSIAVGASTAQQARELIVAT 60 Query: 61 QALTEKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQM 120 +ALT KP+NVNVFCH+PA D R+ WL+HLQPL EFG R LKEIY SF D + Sbjct: 61 RALTSKPFNVNVFCHRPAYSDPLREATWLAHLQPLFAEFGARTPVALKEIYTSFVVDEAL 120 Query: 121 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF 180 QV++E PAVVSFHFGLP + I L +AGILL AT EARQIE AGLDAIVAQG Sbjct: 121 LQVLIEERPAVVSFHFGLPSREWINELHRAGILLFGCATTPHEARQIEQAGLDAIVAQGA 180 Query: 181 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMG 240 EAGGHRG+F+P D IG+ AL+R++ + + LPVIAAGGIMDGQ +A AL LGA AVQMG Sbjct: 181 EAGGHRGVFNPDDDAMIGTLALVRMIARQSTLPVIAAGGIMDGQSVAGALLLGASAVQMG 240 Query: 241 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY 300 TAF+L ES A+A+YR L + RA T VTAAISGRPARG+ NR ++D+P APP+P Y Sbjct: 241 TAFVLCPESGADAAYRTALTTQRAYRTAVTAAISGRPARGLSNRLYIDVDSPDAPPLPDY 300 Query: 301 SAAYDAGKQLHALAAARDVHEFGAHWAGQG 330 AYDA K L+A A A+ EF WAGQG Sbjct: 301 PIAYDAAKALNAAARAKGNTEFAVQWAGQG 330 >ref|YP_004475193.1| 2-nitropropane dioxygenase [Pseudomonas fulva 12-X] gb|AEF23099.1| 2-nitropropane dioxygenase [Pseudomonas fulva 12-X] Length = 350 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 190/343 (55%), Positives = 244/343 (71%), Gaps = 1/343 (0%) Query: 11 LGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEKPYNV 70 LG+ +PIIQAPMAG++T +AAA SNAG LGS+++ A AE R+ + + + T KP+N+ Sbjct: 6 LGIDYPIIQAPMAGTATPALAAAVSNAGGLGSISIAAVTAEAGRQMIRETRVATSKPFNI 65 Query: 71 NVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLEHAPA 130 NVFCH+ +V D+ ++AWL+HLQP EFG +++IY SF D QV+L+ P Sbjct: 66 NVFCHRLSVADAATEQAWLAHLQPYFAEFGAAAPEAIRDIYCSFQEDEGQLQVLLDERPP 125 Query: 131 VVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRGIFD 190 +VSFH GLPP + I AL+QAGI L+A+AT L+E + AG+D IVAQG EAGGHRG+FD Sbjct: 126 IVSFHLGLPPQERIDALKQAGITLLASATTLEEGLLAQDAGIDIIVAQGIEAGGHRGVFD 185 Query: 191 PTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLLTRESN 250 P D+ IG+ AL+R+LV+ + PVIAAGGIMDG GIAA + LGA A Q+GTAF+L ES Sbjct: 186 PAHDQGIGTLALVRILVKQLKRPVIAAGGIMDGAGIAAVMNLGACAAQLGTAFILCPESA 245 Query: 251 ANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYDAGKQL 310 AN +YR +L S RA+HT++T ISGRPARGIVNRFM E+ P P +P + AYDAGKQL Sbjct: 246 ANQAYRQMLGSERARHTQITRVISGRPARGIVNRFMAEVGAPGHPEVPGFGIAYDAGKQL 305 Query: 311 HALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQA 353 A AA EF AHWAGQGAAL R AELV +L +E+ A Sbjct: 306 IAAAAKAGNAEFAAHWAGQGAALART-MPAAELVAVLQREYLA 347 >ref|YP_001579734.1| 2-nitropropane dioxygenase NPD [Burkholderia multivorans ATCC 17616] ref|YP_001946151.1| 2-nitropropane dioxygenase [Burkholderia multivorans ATCC 17616] gb|ABX15237.1| 2-nitropropane dioxygenase NPD [Burkholderia multivorans ATCC 17616] dbj|BAG43615.1| 2-nitropropane dioxygenase [Burkholderia multivorans ATCC 17616] Length = 366 Score = 370 bits (951), Expect = e-100, Method: Compositional matrix adjust. Identities = 204/348 (58%), Positives = 251/348 (72%), Gaps = 2/348 (0%) Query: 7 LMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEK 66 L+++LG+ PIIQAPMAG ST +AAA SNAG LGSL +GA+NA+ AR+ + +ALT++ Sbjct: 10 LLRQLGIRVPIIQAPMAGVSTPALAAAVSNAGGLGSLGVGATNADGARKMIRDTRALTDR 69 Query: 67 PYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLE 126 P+N+NVFCHQPA D+ +RAWL L P E+G A L EIY SF D+ MQ +++E Sbjct: 70 PFNINVFCHQPARADAAIERAWLDWLAPAFREYGATPPASLSEIYTSFVADSAMQAMLVE 129 Query: 127 HAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHR 186 PAVVSFHFGLP ADVI AL++AG+ L A+ATNLDEARQI AG+DAIVAQG EAGGHR Sbjct: 130 EKPAVVSFHFGLPSADVIAALKRAGVTLFASATNLDEARQIADAGIDAIVAQGVEAGGHR 189 Query: 187 GIFDP-TKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLL 245 G+FDP +D+++G+FAL RLLV+ LPVIAAGGIMDG+GIAA L LGAQA Q+GTAF+ Sbjct: 190 GVFDPAARDDRLGTFALTRLLVRECALPVIAAGGIMDGEGIAAVLALGAQAAQLGTAFVA 249 Query: 246 TRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYD 305 E++ + YR + S +HT TAAISGR ARGI NRF D P AP PAY AYD Sbjct: 250 CPETSIDDGYRRAILSDATRHTTFTAAISGRVARGIANRFTALGDDPRAPDTPAYPIAYD 309 Query: 306 AGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQA 353 AGK LHA A A+ +GA WAGQ A L+R AELV L +E +A Sbjct: 310 AGKALHAAAKAKGEFGYGAQWAGQAAPLVR-ALPAAELVATLERETRA 356 >ref|ZP_03545659.1| 2-nitropropane dioxygenase NPD [Comamonas testosteroni KF-1] gb|EED69945.1| 2-nitropropane dioxygenase NPD [Comamonas testosteroni KF-1] Length = 355 Score = 368 bits (945), Expect = e-100, Method: Compositional matrix adjust. Identities = 197/346 (56%), Positives = 247/346 (71%), Gaps = 2/346 (0%) Query: 8 MQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEKP 67 M LGL HPIIQAPMAG+ST +AAA SNA ALGS+ +GA EQAR+ + Q +ALT +P Sbjct: 8 MTGLGLQHPIIQAPMAGTSTPQLAAAVSNAYALGSVGIGAYGLEQARQHIEQTRALTGRP 67 Query: 68 YNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLEH 127 +NVN+FCH+ V D+ER+ WL +L P + +G + + L+ IY+S + ++Q ++LE Sbjct: 68 FNVNLFCHRAPVADAEREAQWLRYLAPEFERYGASVPSGLRCIYESALGNARLQAMLLEL 127 Query: 128 APAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRG 187 PAVVSFHFGLP + ALR AGI +A AT+LDEARQIE AG+D IVAQG EAGGHRG Sbjct: 128 RPAVVSFHFGLPEQSFVDALRAAGITTLACATSLDEARQIERAGVDVIVAQGMEAGGHRG 187 Query: 188 IFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLLTR 247 F P +D +G+ AL+RLL + +RLPV+AAGGIMDG GIAAALQLGA AVQMGTAF+L Sbjct: 188 AFVPEQDTLMGTLALVRLLARESRLPVVAAGGIMDGAGIAAALQLGACAVQMGTAFILCP 247 Query: 248 ESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYDAG 307 ES A+ +YRA L+ AQHT +TAAISGRPARG+ NR + ++ A +PAY YDAG Sbjct: 248 ESAASQAYRAELQGDSAQHTAITAAISGRPARGMFNR-LHQLGRDYAGALPAYPMVYDAG 306 Query: 308 KQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQA 353 K LH A+A+ ++ AHWAGQGA L R AELV L QE A Sbjct: 307 KALHQAASAQGSSDYAAHWAGQGAPLAR-AMPAAELVRTLAQELDA 351 >ref|YP_004485679.1| 2-nitropropane dioxygenase NPD [Delftia sp. Cs1-4] gb|AEF87324.1| 2-nitropropane dioxygenase NPD [Delftia sp. Cs1-4] Length = 357 Score = 368 bits (944), Expect = e-100, Method: Compositional matrix adjust. Identities = 200/352 (56%), Positives = 254/352 (72%), Gaps = 2/352 (0%) Query: 5 TPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALT 64 T M RLG+ HPIIQAPMAG+ST +AAA ++AG LGSL +GAS EQAR+ L Q +ALT Sbjct: 6 TAWMARLGMRHPIIQAPMAGTSTPALAAAVTDAGGLGSLGIGASGTEQARQQLVQTRALT 65 Query: 65 EKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVV 124 +NVN+FCH+PA D++R+ AWL+HL P FG A L EIY + ++ +Q ++ Sbjct: 66 SGAFNVNLFCHRPARADAQREAAWLAHLAPEFARFGAAPPATLNEIYATAVSNAALQAML 125 Query: 125 LEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGG 184 L+ PAVVSFHFGLP I ALR AG++ +A+AT+LDEARQ+E AG+DAIVAQG EAGG Sbjct: 126 LQERPAVVSFHFGLPEQGFIDALRAAGVVTLASATSLDEARQVEQAGVDAIVAQGVEAGG 185 Query: 185 HRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFL 244 HRG FDP +D +G+FAL+R+L ++T LPVIAAGGIMDG+GIAAAL LGA A Q+GTAF+ Sbjct: 186 HRGTFDPAQDRMMGTFALVRVLARNTALPVIAAGGIMDGEGIAAALGLGACAAQLGTAFI 245 Query: 245 LTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAY 304 L ES+A+ +YRA L+S RA HT +TAAISGRPARG+VNR + + P+P Y Y Sbjct: 246 LCPESSASPAYRAELRSERAHHTGITAAISGRPARGMVNR-LHALGASHGQPLPDYPRVY 304 Query: 305 DAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 DAGK LH A+A+ ++ AHWAGQGA L R+ A LV L E + QA Sbjct: 305 DAGKALHQAASAQGSSDYAAHWAGQGAPLARE-MPAAALVQTLAGELASAQA 355 >ref|YP_003276254.1| 2-nitropropane dioxygenase [Comamonas testosteroni CNB-2] gb|ACY30958.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni CNB-2] Length = 348 Score = 368 bits (944), Expect = e-99, Method: Compositional matrix adjust. Identities = 196/345 (56%), Positives = 247/345 (71%), Gaps = 2/345 (0%) Query: 8 MQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEKP 67 M LGL HPIIQAPMAG+ST +AAA SNA ALGS+ +GA EQAR+ + Q +ALT +P Sbjct: 1 MTGLGLQHPIIQAPMAGTSTPQLAAAVSNAYALGSVGIGAYGLEQARQHIEQTRALTNRP 60 Query: 68 YNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLEH 127 +NVN+FCH+ V D+ER+ WL +L P + +G + + L+ IY+S + ++Q ++LE Sbjct: 61 FNVNLFCHRAPVADAEREAQWLRYLAPEFERYGASVPSGLRCIYESALGNERLQAMLLEL 120 Query: 128 APAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRG 187 PAVVSFHFGLP + ALR AGI +A AT+LDEARQIE AG+D IVAQG EAGGHRG Sbjct: 121 RPAVVSFHFGLPEQGFVDALRAAGITTLACATSLDEARQIEQAGVDVIVAQGMEAGGHRG 180 Query: 188 IFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLLTR 247 F P +D +G+ AL+RLL + +RLPV+AAGGIMDG GIAAALQLGA AVQMGTAF+L Sbjct: 181 AFVPEQDTLMGTLALVRLLARESRLPVVAAGGIMDGAGIAAALQLGACAVQMGTAFILCP 240 Query: 248 ESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYDAG 307 ES A+ +YRA L+ AQHT +TAAISGRPARG+ NR + ++ A +PAY YDAG Sbjct: 241 ESAASQAYRAELQGDSAQHTAITAAISGRPARGMFNR-LHQLGRDYAGALPAYPMVYDAG 299 Query: 308 KQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQ 352 K LH A+A+ ++ AHWAGQGA L R AELV L QE + Sbjct: 300 KALHQAASAQGSSDYAAHWAGQGAPLAR-AMPAAELVRTLAQELE 343 >ref|YP_001561310.1| 2-nitropropane dioxygenase NPD [Delftia acidovorans SPH-1] gb|ABX32925.1| 2-nitropropane dioxygenase NPD [Delftia acidovorans SPH-1] Length = 357 Score = 364 bits (935), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 198/352 (56%), Positives = 253/352 (71%), Gaps = 2/352 (0%) Query: 5 TPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALT 64 T M RLG+ HPIIQAPMAG+ST +AAA + AG LGSL +GAS EQAR+ + Q +ALT Sbjct: 6 TAWMARLGMRHPIIQAPMAGTSTPALAAAVTEAGGLGSLGIGASGTEQARQQIVQTRALT 65 Query: 65 EKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVV 124 +NVN+FCH+PA D++R+ AWL+HL P FG + A L EIY + + +Q ++ Sbjct: 66 SGAFNVNLFCHRPARADAQREAAWLAHLAPEFARFGAKPPAGLNEIYATAVGNAALQAML 125 Query: 125 LEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGG 184 L+ PAVVSFHFGLP I ALR AG++ +A+AT+LDEARQ+E AG+DAIVAQG EAGG Sbjct: 126 LQERPAVVSFHFGLPEQGFIDALRAAGVVTLASATSLDEARQVEQAGVDAIVAQGVEAGG 185 Query: 185 HRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFL 244 HRG FDP +D +G+FAL+R+L ++T LPVIAAGGIMDG+GIAAAL LGA A Q+GTAF+ Sbjct: 186 HRGTFDPAQDRMMGTFALVRVLARNTALPVIAAGGIMDGEGIAAALGLGACAAQLGTAFI 245 Query: 245 LTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAY 304 L ES+A+ ++RA L+S RA HT +TAAISGRPARG+VNR + + P+P Y Y Sbjct: 246 LCPESSASPAFRAELRSERAHHTGITAAISGRPARGMVNR-LHALGASHGQPLPDYPRVY 304 Query: 305 DAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 DAGK LH A+A+ ++ AHWAGQGA L R+ A LV L E + QA Sbjct: 305 DAGKALHQAASAQGSSDYAAHWAGQGAPLARE-MPAAALVQTLAGELASAQA 355 >ref|ZP_07047166.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni S44] gb|EFI59197.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni S44] Length = 355 Score = 363 bits (933), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 196/346 (56%), Positives = 245/346 (70%), Gaps = 2/346 (0%) Query: 8 MQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEKP 67 M LGL HPIIQAPMAG+ST +AAA SNA ALGS+ +GA EQAR+ + Q +ALT++P Sbjct: 8 MTGLGLQHPIIQAPMAGTSTPQLAAAVSNAYALGSIGIGAYGLEQARQHIEQTRALTDRP 67 Query: 68 YNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLEH 127 +NVN+FCH+ V D+ER+ WL +L P + +G L+ IY+S + ++Q ++LE Sbjct: 68 FNVNLFCHRAPVADAEREAQWLRYLAPEFERYGASAPPSLRCIYESALGNERLQAMLLEL 127 Query: 128 APAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRG 187 PAVVSFHFGLP + AL AGI +A AT+LDEARQIE AG+D IVAQG EAGGHRG Sbjct: 128 RPAVVSFHFGLPEQGFVDALCAAGITTLACATSLDEARQIEQAGVDVIVAQGMEAGGHRG 187 Query: 188 IFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLLTR 247 F P +D +G+ AL+RLL + +RLPV+AAGGIMDG GIAAALQLGA AVQMGTAF+L Sbjct: 188 AFVPEQDRLMGTLALVRLLARESRLPVVAAGGIMDGAGIAAALQLGACAVQMGTAFILCP 247 Query: 248 ESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYDAG 307 ES A+ +YRA L+ AQHT +TAAISGRPARG+ NR + ++ A +PAY YDAG Sbjct: 248 ESAASQAYRAELQGDSAQHTAITAAISGRPARGMFNR-LHQLGRDYAGALPAYPMVYDAG 306 Query: 308 KQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQA 353 K LH A+A+ ++ AHWAGQGA L R AELV L QE A Sbjct: 307 KALHQAASAQGSSDYAAHWAGQGAPLAR-AMPAAELVRTLAQELDA 351 >ref|YP_299320.1| 2-nitropropane dioxygenase [Ralstonia eutropha JMP134] gb|AAZ64476.1| 2-nitropropane dioxygenase, NPD [Ralstonia eutropha JMP134] Length = 376 Score = 358 bits (920), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 200/347 (57%), Positives = 247/347 (71%), Gaps = 2/347 (0%) Query: 7 LMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALTEK 66 L+ L +A PIIQAPMAG ST MAAA S AG LGSL +GA +AE AR+A+ +ALT K Sbjct: 13 LLSLLNIATPIIQAPMAGVSTPAMAAAVSEAGGLGSLGVGAMDAEGARKAIRDTRALTGK 72 Query: 67 PYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVVLE 126 P+N+N+FCH PAV D+ R++AWL +L P E+G A L+EIY+SF D M Q++LE Sbjct: 73 PFNINLFCHVPAVADAAREKAWLDYLAPRFAEYGATPPASLREIYKSFVVDEAMFQMLLE 132 Query: 127 HAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHR 186 PAVVSFHFGLP + I AL AGI+L+A+AT+L+EARQIEAAG+DAIVAQG EAGGHR Sbjct: 133 EKPAVVSFHFGLPAQEKIDALHAAGIVLLASATSLEEARQIEAAGIDAIVAQGIEAGGHR 192 Query: 187 GIFDPT-KDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLL 245 G+FDP DE +G+ AL+R+LV+HTRLPVIAAGGIMDG GIAA L LGAQA Q+GTAF+ Sbjct: 193 GVFDPAGYDEGLGTLALVRILVRHTRLPVIAAGGIMDGAGIAAVLALGAQAAQLGTAFVG 252 Query: 246 TRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAAYD 305 ES+A+A+YRA + + +T AISGR ARG NRF E + P AP +P Y YD Sbjct: 253 CEESSADAAYRAAFSAQPVRPPTLTRAISGRAARGFTNRFTELGEAPDAPAVPDYPTTYD 312 Query: 306 AGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQ 352 AGK LHA A A+ ++ A W+GQ A L R AELV L E + Sbjct: 313 AGKALHAAAKAKGNSDYAAQWSGQAAPLARQ-LPAAELVGQLQAELR 358