BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0135.p (263 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8ITH1.1 D8ITH1_HERSS Enoyl-CoA hydratase II protein EC=4.2.1.17 533 e-149 A6F422.1 A6F422_9ALTE Enoyl-CoA hydratase EC=4.2.1.17 377 e-102 E4PKI0.1 E4PKI0_MARAH Phenylacetate degradation probable enoyl-C... 369 e-100 A4TVC1.1 A4TVC1_9PROT Enoyl-CoA hydratase/isomerase 362 4e-98 A3JBF1.1 A3JBF1_9ALTE Enoyl-CoA hydratase EC=4.2.1.17 353 1e-95 A3YFA9.1 A3YFA9_9GAMM Enoyl-CoA hydratase EC=4.2.1.17 344 9e-93 Q4KBZ7.1 Q4KBZ7_PSEF5 Phenylacetic acid degradation protein PaaB 340 1e-91 Q70IM8.1 Q70IM8_9PSED Putative enoyl-CoA hydratase II 336 2e-90 B1J8Q5.1 B1J8Q5_PSEPW Phenylacetate degradation, enoyl-CoA hydra... 335 5e-90 B0KSI7.1 B0KSI7_PSEPG Phenylacetate degradation, enoyl-CoA hydra... 334 8e-90 >D8ITH1.1 D8ITH1_HERSS Enoyl-CoA hydratase II protein EC=4.2.1.17 Length = 263 Score = 533 bits (1372), Expect = e-149, Method: Compositional matrix adjust. Identities = 262/263 (99%), Positives = 262/263 (99%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDLGDRAVKPGDD VDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL Sbjct: 61 FCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG Sbjct: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG Sbjct: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 RSRDYREGVTAFLEKRAPQFTGQ Sbjct: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 >A6F422.1 A6F422_9ALTE Enoyl-CoA hydratase EC=4.2.1.17 Length = 263 Score = 377 bits (967), Expect = e-102, Method: Compositional matrix adjust. Identities = 177/263 (67%), Positives = 210/263 (79%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ PSILLE G+A +TLNRPD LNSF V MH ++R AI+ ++ D SVRVL++TGAGRG Sbjct: 1 MTQPSILLEIDQGVAQLTLNRPDNLNSFNVEMHEQMRKAISQVRKDSSVRVLVITGAGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR+V P DA DLGES++KYY P+++SLR LP PV+CAVNGVAAGAGAN+ L Sbjct: 61 FCAGQDLSDRSVAPDQDAPDLGESLEKYYNPMMRSLRDLPMPVLCAVNGVAAGAGANIAL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACDI LAARSASFV+ FCKLGLIPD+GGT+ LPR+ G ARA GMALLG+KI AEQAE WG Sbjct: 121 ACDITLAARSASFVQAFCKLGLIPDSGGTWTLPRIAGMARAKGMALLGDKIRAEQAENWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 +IW+CVDD+ LMEE +A HFA PTKGLA K L+AS ++T EQL+LERD R G Sbjct: 181 MIWRCVDDETLMEETMKLARHFATQPTKGLALIKRALHASSSNTFEEQLNLERDLQRMAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 RS DYREGV AF+EKR P F G+ Sbjct: 241 RSEDYREGVAAFMEKRTPTFKGK 263 >E4PKI0.1 E4PKI0_MARAH Phenylacetate degradation probable enoyl-CoA hydratase paaB Length = 263 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 170/263 (64%), Positives = 211/263 (80%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ PSILLE G+A++TLNRPD LNSF V MH +R+AI+T++ D SVRVL++TG+GRG Sbjct: 1 MTQPSILLEIDQGVALLTLNRPDNLNSFNVEMHERMREAISTVRKDESVRVLVITGSGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR+V P + DLG S++ YY PL++SLR LP PV+CAVNGVAAGAGAN+ L Sbjct: 61 FCAGQDLSDRSVSPDQEMPDLGASLENYYNPLMRSLRDLPMPVLCAVNGVAAGAGANIAL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACDI LAARSA+FV+ FCKLGL+PD+GGT+ LPR+ G ARA GMALLG+KISAEQAE WG Sbjct: 121 ACDITLAARSANFVQAFCKLGLVPDSGGTWTLPRVAGMARAKGMALLGDKISAEQAENWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 +IW+CVD++QLMEE +A HFA PTKGLA K L+AS ++T EQ++LERD R G Sbjct: 181 MIWRCVDNEQLMEETMKLARHFATQPTKGLALIKRALHASASNTFEEQINLERDLQRMAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 ++ DYREGV AF+EKR P F G+ Sbjct: 241 QTEDYREGVAAFMEKRTPNFKGK 263 >A4TVC1.1 A4TVC1_9PROT Enoyl-CoA hydratase/isomerase Length = 262 Score = 362 bits (928), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 174/260 (66%), Positives = 208/260 (80%), Gaps = 3/260 (1%) Query: 5 SILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPS--VRVLLLTGAGRGFC 62 +IL + +G+A +TLNRPD+LNSF MH EL+DA+ +Q DP+ VR LL+TGAGRGFC Sbjct: 3 TILFQIENGVATLTLNRPDRLNSFNTDMHAELKDALKQVQ-DPANKVRCLLITGAGRGFC 61 Query: 63 AGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLAC 122 AGQDL DRAV PG+DA DLGESIDK Y PL+++LRAL PVICAVNGVAAGAGANL AC Sbjct: 62 AGQDLSDRAVAPGNDAPDLGESIDKRYNPLIRTLRALEMPVICAVNGVAAGAGANLAFAC 121 Query: 123 DIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLI 182 DIVLAARSASF++ FCK+GL+PD+GGT+ LPRLVG ARA+ +++LG+K+SAEQAE WG+I Sbjct: 122 DIVLAARSASFIQAFCKIGLVPDSGGTWTLPRLVGQARAVALSMLGDKVSAEQAEAWGMI 181 Query: 183 WKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRS 242 WKCVDDDQL EA +AA A PTKGLA K L AS +TL +QLDLERD R GR+ Sbjct: 182 WKCVDDDQLQAEAGKLAAQLATQPTKGLALIKRALAASSTNTLDQQLDLERDLQRLAGRT 241 Query: 243 RDYREGVTAFLEKRAPQFTG 262 RDY+EGV AF+EKR P F G Sbjct: 242 RDYQEGVAAFMEKRVPTFQG 261 >A3JBF1.1 A3JBF1_9ALTE Enoyl-CoA hydratase EC=4.2.1.17 Length = 263 Score = 353 bits (907), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 170/263 (64%), Positives = 206/263 (78%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ S+LL+ GIA +TLNRPD LNSF V MH ++R A+ +++ D SVRV+LLTG GRG Sbjct: 1 MTEQSVLLDIDQGIAKVTLNRPDSLNSFNVEMHKQMRAALKSIRNDASVRVMLLTGNGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR VKPG DLG SI++YY PL+++LR LP PVICAVNGVAAGAGAN+ L Sbjct: 61 FCAGQDLSDRTVKPGAQVPDLGISIEQYYNPLLQALRDLPMPVICAVNGVAAGAGANIAL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACDI LAARSA+FV+ FCK+GLIPD GGT+ LPR VG ARA G+ALLG K+SAE+AE+WG Sbjct: 121 ACDITLAARSANFVQAFCKIGLIPDCGGTWTLPRAVGMARAKGLALLGGKLSAEKAEQWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 +IW+CV+DD L +EA +A HFA PTKGLA K L+AS +T EQ +ERD R G Sbjct: 181 MIWQCVEDDALQDEAMNLARHFATQPTKGLAMIKRALHASANNTFEEQSLVERDLQRLAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 RS DYREGV+AF EKRAP+FTG+ Sbjct: 241 RSDDYREGVSAFTEKRAPKFTGK 263 >A3YFA9.1 A3YFA9_9GAMM Enoyl-CoA hydratase EC=4.2.1.17 Length = 268 Score = 344 bits (882), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 161/259 (62%), Positives = 200/259 (77%) Query: 5 SILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAG 64 +IL GIA+ITLNRPD+LNSF MH E+R A+ ++ D SVRVL++TGAGRGFCAG Sbjct: 10 TILFSINKGIALITLNRPDQLNSFNSQMHDEMRSALKQVEKDMSVRVLVITGAGRGFCAG 69 Query: 65 QDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDI 124 QDL DR V PG DLG+S++K Y P+V+ ++ALP PVICAVNGVAAGAGAN+ LACDI Sbjct: 70 QDLSDRNVAPGAHTPDLGDSLEKRYNPMVRKIKALPMPVICAVNGVAAGAGANIALACDI 129 Query: 125 VLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWK 184 VLAA+SASF++ FCKLGLIPD+GGT+ LP+ +G ARA G+ALLG+K+SAEQAE WG+IW+ Sbjct: 130 VLAAKSASFIQAFCKLGLIPDSGGTWSLPKTLGMARAKGLALLGDKVSAEQAEAWGMIWR 189 Query: 185 CVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRD 244 +D++ M+E MA HF+ PTKGLA K L+AS + EQLDLERD R GRS D Sbjct: 190 AIDNESFMDEVMQMAHHFSTQPTKGLALIKRALHASEETSFDEQLDLERDLQRLAGRSSD 249 Query: 245 YREGVTAFLEKRAPQFTGQ 263 Y+EGV AF+EKR FTG+ Sbjct: 250 YQEGVAAFIEKRPANFTGK 268 >Q4KBZ7.1 Q4KBZ7_PSEF5 Phenylacetic acid degradation protein PaaB Length = 263 Score = 340 bits (873), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 164/262 (62%), Positives = 205/262 (78%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ +IL +G+A+++LNRP++LNSF MH E++ A+ ++ DP VRVLLLTG GRG Sbjct: 1 MNFTTILFSISEGVALLSLNRPEQLNSFNPTMHREVQAALDLVRNDPQVRVLLLTGEGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDLG+R V PG A DLG SI+ +Y PLV++LR LP PVICAVNGVAAGAGANLPL Sbjct: 61 FCAGQDLGERTVAPGGAAPDLGASIETFYNPLVRALRDLPLPVICAVNGVAAGAGANLPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACD+VLAARSASF++ FCKLGL+PD+GGT+ LPRLVG ARA +ALLG ++SAEQAE+WG Sbjct: 121 ACDLVLAARSASFIQAFCKLGLVPDSGGTWLLPRLVGMARAKALALLGNRLSAEQAEQWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LI++CVDD QL ++A ++A H A PT GL K +L AS ++ EQLDLERD R G Sbjct: 181 LIYQCVDDAQLRDQALSLARHLATQPTYGLGLIKRSLNASLHNSFDEQLDLERDLQRLAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTG 262 S DYREGV+AF+EKR+P F G Sbjct: 241 CSEDYREGVSAFMEKRSPVFKG 262 >Q70IM8.1 Q70IM8_9PSED Putative enoyl-CoA hydratase II Length = 263 Score = 336 bits (862), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 160/262 (61%), Positives = 200/262 (76%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 MS IL G+A ++LNRP++LNSF MH E+R+A+ T+Q + VRVLLLTG GRG Sbjct: 1 MSYQHILFSIEAGVATLSLNRPEQLNSFNTLMHQEVREALKTVQNNSDVRVLLLTGEGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR V PG + DLG+SI+++Y PL+++LR LP PVICAVNGVAAGAGAN+PL Sbjct: 61 FCAGQDLSDRNVAPGAEMPDLGQSIERFYNPLIRTLRELPIPVICAVNGVAAGAGANIPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACD+VLAARSASF++ FCK+GLIPD+ GT+ LPRLVG ARA + LLGE++SAEQA WG Sbjct: 121 ACDLVLAARSASFIQAFCKIGLIPDSAGTWALPRLVGMARAKALTLLGERLSAEQALEWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LI++ VDD +L ++A A+A A PT GL K +L+AS ++ +QL LERD R G Sbjct: 181 LIYRVVDDAELRDQAIALARQLATQPTYGLGLIKRSLHASLDNSFEQQLTLERDLQRLAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTG 262 RS DYREGV AF+EKRAPQF G Sbjct: 241 RSEDYREGVAAFMEKRAPQFKG 262 >B1J8Q5.1 B1J8Q5_PSEPW Phenylacetate degradation, enoyl-CoA hydratase paaB Length = 263 Score = 335 bits (858), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 162/262 (61%), Positives = 200/262 (76%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ IL DG+A+++LNRPD+LNSF MH E+R+A+ ++ DP VRVLLLTG GRG Sbjct: 1 MAFEHILFSIEDGVALLSLNRPDQLNSFNTQMHKEVREALKQVRQDPQVRVLLLTGEGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR V PG DLGESI+ +Y PLV++LR LP PVICAVNGVAAGAGAN+PL Sbjct: 61 FCAGQDLSDRNVAPGAQVPDLGESIEAFYNPLVRALRELPMPVICAVNGVAAGAGANIPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACD+VLAARSASF++ FCK+GL+PD+GGT+ LPRLVG ARA +ALLG+++SAE+AE+WG Sbjct: 121 ACDLVLAARSASFIQAFCKIGLVPDSGGTWQLPRLVGMARAKALALLGDRLSAEKAEQWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LI++ V+D L +EA A+A A PT GLA K L AS + +QL+LERD R G Sbjct: 181 LIYQVVEDAALRDEALALARQLATQPTYGLALIKRALNASFDNHFDQQLELERDLQRLAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTG 262 RS DYREGV AF+ KRAP F G Sbjct: 241 RSEDYREGVAAFMAKRAPAFKG 262 >B0KSI7.1 B0KSI7_PSEPG Phenylacetate degradation, enoyl-CoA hydratase paaB Length = 263 Score = 334 bits (856), Expect = 8e-90, Method: Compositional matrix adjust. Identities = 160/263 (60%), Positives = 200/263 (76%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 M+ IL DG+A+++LNRP++LNSF AMH+E+R+A+ ++ RVLLLT GRG Sbjct: 1 MTFQHILFSIEDGVALLSLNRPEQLNSFNTAMHMEVREALKQVRQSSEARVLLLTAEGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDL DR V PG + DLGESIDK+Y PLV++LR LP PVICAVNGVAAGAGAN+PL Sbjct: 61 FCAGQDLSDRNVAPGAEIPDLGESIDKFYNPLVRTLRDLPLPVICAVNGVAAGAGANIPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACD+VLAARSASF++ FCK+GL+PD+GGT+ LPRLVG ARA +A+LGE++ AEQA++WG Sbjct: 121 ACDLVLAARSASFIQAFCKIGLVPDSGGTWLLPRLVGMARAKALAMLGERLGAEQAQQWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LI + VDD L +EA A+A A PT GLA K +L AS + EQL+LERD R G Sbjct: 181 LIHRVVDDAALRDEALALARQLATQPTYGLALIKRSLNASFDNGFDEQLELERDLQRLAG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 RS DYREGV+AF+ KR P F G+ Sbjct: 241 RSEDYREGVSAFMNKRTPVFKGR 263