BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0075.p (145 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8ISV4.1 D8ISV4_HERSS Large-conductance mechanosensitive channel 286 8e-76 A4G9H5.1 MSCL_HERAR Large-conductance mechanosensitive channel 199 9e-50 F1VX71.1 F1VX71_9BURK Large-conductance mechanosensitive channel 195 1e-48 E7S0Z2.1 E7S0Z2_9BURK Large conductance mechanosensitive channel... 181 4e-44 A2SE21.1 MSCL_METPP Large-conductance mechanosensitive channel 179 1e-43 A6T365.1 MSCL_JANMA Large-conductance mechanosensitive channel 175 2e-42 F0L609.1 F0L609_9RHIZ Large conductance mechanosensitive channel... 174 5e-42 Q8UHY0.1 MSCL_AGRT5 Large-conductance mechanosensitive channel 172 1e-41 B1Y2I5.1 MSCL_LEPCP Large-conductance mechanosensitive channel 172 2e-41 Q11M81.1 Q11M81_MESSB Large-conductance mechanosensitive channel 172 2e-41 >D8ISV4.1 D8ISV4_HERSS Large-conductance mechanosensitive channel Length = 145 Score = 286 bits (732), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 145/145 (100%), Positives = 145/145 (100%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP Sbjct: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA Sbjct: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 Query: 121 PAAPAPTPEDVLLLREIRDSLKKQG 145 PAAPAPTPEDVLLLREIRDSLKKQG Sbjct: 121 PAAPAPTPEDVLLLREIRDSLKKQG 145 >A4G9H5.1 MSCL_HERAR Large-conductance mechanosensitive channel Length = 137 Score = 199 bits (507), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 103/144 (71%), Positives = 120/144 (83%), Gaps = 8/144 (5%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 M M+++F+AFA+KGNVVDLAV VIIGGAFGKIV SLV+DIIMP+VGKIFGGLDF+NYYLP Sbjct: 1 MGMMQEFKAFAVKGNVVDLAVAVIIGGAFGKIVDSLVQDIIMPVVGKIFGGLDFSNYYLP 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 LN Q L L EA+K GAVFAYG+F+TILINFLILAFIIFQMVR + SK + + Sbjct: 61 LNNQDISLTLVEAKKLGAVFAYGSFITILINFLILAFIIFQMVR-------MLSKLKRSE 113 Query: 121 PAAPAP-TPEDVLLLREIRDSLKK 143 P A AP TPE+V+LLREIRD+LKK Sbjct: 114 PPAEAPATPEEVVLLREIRDALKK 137 >F1VX71.1 F1VX71_9BURK Large-conductance mechanosensitive channel Length = 139 Score = 195 bits (496), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 101/143 (70%), Positives = 118/143 (82%), Gaps = 4/143 (2%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 M M+++F+ FA+KGNV+DLAVGVIIG AFGKIV S+V+D+IMPIV IFGGLDFANYY+P Sbjct: 1 MGMMQEFKTFAVKGNVMDLAVGVIIGAAFGKIVDSVVQDLIMPIVSTIFGGLDFANYYIP 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 LNGQ L L EA+KAGAV AYGNFLTI INFLILAFIIFQM+R +N AR + + A Sbjct: 61 LNGQGMQLSLVEAKKAGAVLAYGNFLTISINFLILAFIIFQMIRLVNAAR----RKQPAP 116 Query: 121 PAAPAPTPEDVLLLREIRDSLKK 143 APA TPEDVLLLREIRD+L+K Sbjct: 117 APAPAVTPEDVLLLREIRDALRK 139 >E7S0Z2.1 E7S0Z2_9BURK Large conductance mechanosensitive channel protein Length = 146 Score = 181 bits (458), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 102/147 (69%), Positives = 116/147 (78%), Gaps = 7/147 (4%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 MSML +FR FA+KGNVVDLAVGVIIGGAFGKIV S+V+DIIMPIVG IFGGLDF++Y++P Sbjct: 1 MSMLTEFRDFALKGNVVDLAVGVIIGGAFGKIVDSMVKDIIMPIVGAIFGGLDFSSYFIP 60 Query: 61 LNGQ-AYGLPLA-EA-RKAG-AVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKH 116 L + GLP EA KAG A+ AYGNFLTI +NFLILAFIIFQMV NKA+ K Sbjct: 61 LTTKIPEGLPHTYEAYTKAGVAILAYGNFLTIFLNFLILAFIIFQMVNVFNKAKTALIKA 120 Query: 117 EEAAPAAPAPTPEDVLLLREIRDSLKK 143 E + PAAP PEDV+LLREIRD LKK Sbjct: 121 EASKPAAP---PEDVVLLREIRDELKK 144 >A2SE21.1 MSCL_METPP Large-conductance mechanosensitive channel Length = 137 Score = 179 bits (453), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 94/143 (65%), Positives = 116/143 (81%), Gaps = 6/143 (4%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 MS +F+ FA+KGNV+DLAVGVIIGGAFGKIV S+V D+IMPIV K+FGGLDF+NYY+ Sbjct: 1 MSFFSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSIVGDLIMPIVSKLFGGLDFSNYYVG 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 L GQA GLPLAEA+KAGAVFAYGNF+T+ +NF ILAFIIF M++ IN+ + ++ Sbjct: 61 LAGQAAGLPLAEAKKAGAVFAYGNFITVALNFAILAFIIFLMIKQINRLK------KDEP 114 Query: 121 PAAPAPTPEDVLLLREIRDSLKK 143 A PAP ED++LLREIRD+LKK Sbjct: 115 AAPPAPPAEDIVLLREIRDALKK 137 >A6T365.1 MSCL_JANMA Large-conductance mechanosensitive channel Length = 139 Score = 175 bits (443), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 95/143 (66%), Positives = 114/143 (79%), Gaps = 4/143 (2%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 M M+++F+ FA+KGNVVDLAV VI+G AFGKIV SLV+D+IMP+VGKIFGGLDF+NYY+ Sbjct: 1 MGMMQEFKEFAVKGNVVDLAVAVIVGAAFGKIVDSLVQDVIMPVVGKIFGGLDFSNYYIA 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 LN Q L L EA+K GAV AYGNF+TI +NF+ILAFIIFQMVR +NK + + E A Sbjct: 61 LNNQDPNLTLVEAKKLGAVLAYGNFITIALNFIILAFIIFQMVRLLNKLK----RTEPPA 116 Query: 121 PAAPAPTPEDVLLLREIRDSLKK 143 P PA PED+ LLREIRDSLKK Sbjct: 117 PEEPAAVPEDIALLREIRDSLKK 139 >F0L609.1 F0L609_9RHIZ Large conductance mechanosensitive channel protein Length = 142 Score = 174 bits (440), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 87/142 (61%), Positives = 104/142 (73%) Query: 3 MLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLPLN 62 ML +F+ F +GNV+DLAVGVIIG AF KIV S+V D+IMPIVG IFGG DF+NY+LPL+ Sbjct: 1 MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLS 60 Query: 63 GQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAAPA 122 LA AR GAVFAYGNFLT+LINFLILA+IIF MV+ +NK R + + + Sbjct: 61 SSVTASSLAAARDQGAVFAYGNFLTVLINFLILAWIIFLMVKGVNKLRASVDRKKIEEKS 120 Query: 123 APAPTPEDVLLLREIRDSLKKQ 144 PAP PEDV LL EIRD LK + Sbjct: 121 EPAPPPEDVKLLTEIRDLLKTR 142 >Q8UHY0.1 MSCL_AGRT5 Large-conductance mechanosensitive channel Length = 142 Score = 172 bits (437), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 90/143 (62%), Positives = 107/143 (74%), Gaps = 2/143 (1%) Query: 3 MLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLPLN 62 ML +F+ F +GNV+DLAVGVIIG AF KIV S+V D+IMPIVG IFGG DF+NY+LPL+ Sbjct: 1 MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLS 60 Query: 63 GQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHE-EAAP 121 LA AR+ GAVFAYGNFLT+LINFLILA+IIF MV+ +NK RD + + E P Sbjct: 61 SNVNATSLAAAREQGAVFAYGNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKP 120 Query: 122 AAPAPTPEDVLLLREIRDSLKKQ 144 A AP PEDV LL EIRD LK + Sbjct: 121 DA-APPPEDVKLLTEIRDLLKTR 142 >B1Y2I5.1 MSCL_LEPCP Large-conductance mechanosensitive channel Length = 140 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 94/143 (65%), Positives = 116/143 (81%), Gaps = 3/143 (2%) Query: 1 MSMLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLP 60 MS+L +F+AFA+KGNVVDLAVGVIIGGAFGKIV SLV D+IMPIV KIFGGLDF+NY++P Sbjct: 1 MSVLSEFKAFAVKGNVVDLAVGVIIGGAFGKIVESLVGDVIMPIVSKIFGGLDFSNYFIP 60 Query: 61 LNGQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAA 120 L GQ L EA+KAGAV AYG+F+T+ INF+ILAFIIF M++ IN+ + ++ A Sbjct: 61 LAGQT-ATTLVEAKKAGAVLAYGSFITVAINFMILAFIIFMMIKQINRLQ--SAPAPAPA 117 Query: 121 PAAPAPTPEDVLLLREIRDSLKK 143 PA P P ED++LLREIRDSLK+ Sbjct: 118 PAEPPPPAEDIVLLREIRDSLKR 140 >Q11M81.1 Q11M81_MESSB Large-conductance mechanosensitive channel Length = 142 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 86/142 (60%), Positives = 107/142 (75%), Gaps = 4/142 (2%) Query: 3 MLKDFRAFAIKGNVVDLAVGVIIGGAFGKIVSSLVEDIIMPIVGKIFGGLDFANYYLPLN 62 ML +FR F +GNV+DLAVGVIIGGAFG IVSSL +DIIMPIVG +FGG DF+NY++PL Sbjct: 5 MLNEFREFIARGNVIDLAVGVIIGGAFGGIVSSLTDDIIMPIVGAVFGGFDFSNYFVPLA 64 Query: 63 GQAYGLPLAEARKAGAVFAYGNFLTILINFLILAFIIFQMVRAINKARDLASKHEEAAPA 122 LA+AR+ GAV AYGNF+T+L+NFLILA+IIF MVRA+N+ R + + PA Sbjct: 65 SGVTADTLAQAREQGAVLAYGNFITVLLNFLILAWIIFLMVRAVNRLR----RRQAEQPA 120 Query: 123 APAPTPEDVLLLREIRDSLKKQ 144 PA P+DV LL EIRD L ++ Sbjct: 121 EPAAPPQDVQLLTEIRDLLARR 142