BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0029.p (170 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8ISP2.1 D8ISP2_HERSS Protocatechuate 3,4-dioxygenase alpha chai... 348 2e-94 Q3KGU2.1 Q3KGU2_PSEPF Protocatechuate 3,4-dioxygenase alpha chai... 118 3e-25 Q4KH33.1 Q4KH33_PSEF5 Protocatechuate 3,4-dioxygenase, alpha sub... 113 7e-24 C3KAN6.1 C3KAN6_PSEFS Protocatechuate 3,4-dioxygenase alpha chai... 113 8e-24 F0C1J3.1 F0C1J3_9XANT Protocatechuate 3,4-dioxygenase, alpha sub... 111 4e-23 E2XN12.1 E2XN12_PSEFL Protocatechuate 3,4-dioxygenase, alpha sub... 111 4e-23 F0BFP5.1 F0BFP5_9XANT Protocatechuate 3,4-dioxygenase, alpha sub... 110 5e-23 Q8PDH0.1 Q8PDH0_XANCP Protocatechuate 3,4-dioxygenase alpha chain 110 7e-23 Q4UZQ8.1 Q4UZQ8_XANC8 Protocatechuate 3,4-dioxygenase alpha chain 110 7e-23 B0RMP0.1 B0RMP0_XANCB Protocatechuate 3,4-dioxygenase alpha chai... 110 7e-23 >D8ISP2.1 D8ISP2_HERSS Protocatechuate 3,4-dioxygenase alpha chain protein EC=1.13.11.3 Length = 170 Score = 348 bits (892), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 170/170 (100%), Positives = 170/170 (100%) Query: 1 MSNITTSQTIGPFPHEAWAWAVELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPG 60 MSNITTSQTIGPFPHEAWAWAVELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPG Sbjct: 1 MSNITTSQTIGPFPHEAWAWAVELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPG 60 Query: 61 SAPVESAHAIPGYRRVPTNEEGGFAFSISQPQAPAGKPVAYVTVFARGLVKHQFTAVFLE 120 SAPVESAHAIPGYRRVPTNEEGGFAFSISQPQAPAGKPVAYVTVFARGLVKHQFTAVFLE Sbjct: 61 SAPVESAHAIPGYRRVPTNEEGGFAFSISQPQAPAGKPVAYVTVFARGLVKHQFTAVFLE 120 Query: 121 DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI 170 DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI Sbjct: 121 DDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGARETVFFDYI 170 >Q3KGU2.1 Q3KGU2_PSEPF Protocatechuate 3,4-dioxygenase alpha chain EC=1.13.11.3 Length = 188 Score = 118 bits (296), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 77/188 (40%), Positives = 97/188 (51%), Gaps = 28/188 (14%) Query: 5 TTSQTIGPFPHEAWAW------AVELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWL 58 TTS T+GP+ H W A ELT +V I G ++DG+G +NDA E W Sbjct: 6 TTSHTVGPYYHIGLTWLNRENLANELTL-----GQRVAITGQVVDGNGDVVNDAMLEVWQ 60 Query: 59 PGSAPV----ESAHAIP------GYRRVPTNEEGGFAFSISQPQAPAG------KPVAYV 102 +A E A P G+ RVP + +G F F+ +P G P V Sbjct: 61 ANAAGKYDHPEDEQAKPLDPNFEGFGRVPVDADGRFRFTTIKPGTVEGLKGTTQAPHLVV 120 Query: 103 TVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGAR 162 VFARGLVKH T ++ E DPA LLE VPA+RRDTL+A+ G Y WN+ +QG Sbjct: 121 LVFARGLVKHLLTRIYFEGDPANVNDPLLECVPAERRDTLLAKPDAAGVYQWNVILQGTD 180 Query: 163 -ETVFFDY 169 ETVFFDY Sbjct: 181 AETVFFDY 188 >Q4KH33.1 Q4KH33_PSEF5 Protocatechuate 3,4-dioxygenase, alpha subunit EC=1.13.11.3 Length = 188 Score = 113 bits (283), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 72/183 (39%), Positives = 94/183 (51%), Gaps = 18/183 (9%) Query: 5 TTSQTIGPFPHEAWAWA-VELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPGSAP 63 TTS T+GP+ H W E + + +V I G ++DG+G +NDA E W +A Sbjct: 6 TTSHTVGPYYHIGLTWLNREDLTEEGTLGERVAITGQVVDGNGNFVNDAMLEVWQANAAG 65 Query: 64 V----ESAHAIP------GYRRVPTNEEGGFAFSISQPQAPAG------KPVAYVTVFAR 107 E P G+ RVP + EG F F+ +P G P V VFAR Sbjct: 66 KYRHPEDDQDKPLDPNFQGFGRVPVDAEGRFRFTTIKPGTVPGLKGTTQAPHLVVLVFAR 125 Query: 108 GLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGAR-ETVF 166 GLVKH T ++ E +PA A LL VP +RR TL+A+ DG Y WN+ +QG ETVF Sbjct: 126 GLVKHLLTRIYFEGEPANASDPLLACVPEERRGTLLAKADADGHYQWNVILQGTDAETVF 185 Query: 167 FDY 169 FDY Sbjct: 186 FDY 188 >C3KAN6.1 C3KAN6_PSEFS Protocatechuate 3,4-dioxygenase alpha chain EC=1.13.11.3 Length = 188 Score = 113 bits (283), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 71/185 (38%), Positives = 96/185 (51%), Gaps = 18/185 (9%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVDSS-APQVKINGAILDGDGVPINDAWAEAWLPGS 61 N TTS T+GP+ H W V ++ +V I+G ++DG+G +NDA E W + Sbjct: 4 NATTSHTVGPYYHIGLTWLNREDLTVAATLGERVAISGQVVDGNGDVVNDAMLEVWQANA 63 Query: 62 A-----PVESAHA-----IPGYRRVPTNEEGGFAFSISQPQAPAG------KPVAYVTVF 105 A P + G+ RVP + EG F F+ +P A G P V VF Sbjct: 64 AGKYDHPEDDQDKALDPNFEGFGRVPVDAEGRFRFTTIKPGAVPGLKGTTQAPHLVVLVF 123 Query: 106 ARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGA-RET 164 ARGLVKH T ++ E + LL VPA+RR TLIA+ DG + WN+ +QG +ET Sbjct: 124 ARGLVKHLLTRIYFEGEALNGDDPLLACVPAERRGTLIAKTDADGVHQWNVILQGTDKET 183 Query: 165 VFFDY 169 VFFDY Sbjct: 184 VFFDY 188 >F0C1J3.1 F0C1J3_9XANT Protocatechuate 3,4-dioxygenase, alpha subunit Length = 187 Score = 111 bits (277), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 75/190 (39%), Positives = 103/190 (54%), Gaps = 29/190 (15%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVDSSAP------QVKINGAILDGDGVPINDAWAEA 56 + T SQT+GP+ + +E R D AP QV+I+G + DG G P+ DA E Sbjct: 4 HTTPSQTVGPY----YRLGLEPLYR-DQLAPAQATGTQVQISGCVFDGAGTPVADAVLEL 58 Query: 57 WLPGSAPVESAHA-----------IPGYRRVPTNEEGGFAFSISQP---QAPAGKPVA-- 100 W +A + AHA G+ RVPT+ +G F+FS +P P GK A Sbjct: 59 WQADAAGI-YAHAADTRFETHDPGFDGWGRVPTDAQGCFSFSTIKPGQVAGPDGKLQAAH 117 Query: 101 -YVTVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQ 159 V VF RGL++ T ++ DDP L +L VPA+RR TL+A+ + G+Y+W+IHMQ Sbjct: 118 LTVLVFMRGLLRGVSTRLYFADDPQLRSDPILALVPAERRATLLAQSRGSGNYVWDIHMQ 177 Query: 160 GARETVFFDY 169 G ETVFF Y Sbjct: 178 GDAETVFFHY 187 >E2XN12.1 E2XN12_PSEFL Protocatechuate 3,4-dioxygenase, alpha subunit EC=1.13.11.3 Length = 188 Score = 111 bits (277), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 72/184 (39%), Positives = 97/184 (52%), Gaps = 20/184 (10%) Query: 5 TTSQTIGPFPHEAWAWA--VELTSRVDSSAPQVKINGAILDGDGVPINDAWAEAWLPGSA 62 TTS T+GP+ H W +LTS + +V I+G ++DG+G +NDA E W +A Sbjct: 6 TTSHTVGPYYHIGLTWLNREDLTSAA-TLGERVAISGQVVDGNGDAVNDAMLEVWQANAA 64 Query: 63 PV------ESAHAI----PGYRRVPTNEEGGFAFSISQPQAPAG------KPVAYVTVFA 106 E A+ G+ RVP + EG F F+ +P + G P V VFA Sbjct: 65 GKYDHPEDEQDKAVDPNFEGFGRVPVDAEGRFRFTTIKPGSVPGLKGTTQAPHLVVLVFA 124 Query: 107 RGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGA-RETV 165 RGLVKH T ++ + + LL VPA RR TLIA+ DG Y WN+ +QG +ETV Sbjct: 125 RGLVKHLLTRIYFDGEALNGDDPLLACVPAQRRGTLIAKPGDDGVYQWNVILQGTDKETV 184 Query: 166 FFDY 169 FFDY Sbjct: 185 FFDY 188 >F0BFP5.1 F0BFP5_9XANT Protocatechuate 3,4-dioxygenase, alpha subunit Length = 187 Score = 110 bits (276), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 74/189 (39%), Positives = 98/189 (51%), Gaps = 27/189 (14%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVDSSAP------QVKINGAILDGDGVPINDAWAEA 56 + T SQT+GP+ + +E R D AP QV I+G + DG G P+ DA E Sbjct: 4 HATPSQTVGPY----YRLGLEPLYR-DKIAPAHATGTQVHISGCVFDGAGTPVADAVLEL 58 Query: 57 WLPGSAPV----------ESAHAIPGYRRVPTNEEGGFAFSISQPQA---PAGKPVA--- 100 W +A + + G+ RVPT+ +G F FS +P A P GK A Sbjct: 59 WQADAAGIYAHAADARSAAHDASFDGWGRVPTDAQGRFRFSTVKPGAVAGPEGKAQAAHL 118 Query: 101 YVTVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQG 160 V VF RGL++ T ++ DDP L +L VPA RRDTL+A+ +G Y W+IHMQG Sbjct: 119 TVLVFMRGLLRCASTRLYFADDPQLGSDPILALVPAARRDTLLAQPTGNGVYAWDIHMQG 178 Query: 161 ARETVFFDY 169 ETVFF Y Sbjct: 179 EMETVFFRY 187 >Q8PDH0.1 Q8PDH0_XANCP Protocatechuate 3,4-dioxygenase alpha chain Length = 187 Score = 110 bits (275), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 70/188 (37%), Positives = 103/188 (54%), Gaps = 25/188 (13%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVD-----SSAPQVKINGAILDGDGVPINDAWAEAW 57 + T SQT+GP+ + +E R ++ QV+I+G + DG G+P++DA E W Sbjct: 4 HATPSQTVGPY----YRLGLEPLYRAQIAPEHAAGTQVQISGCVFDGAGMPVSDAVLELW 59 Query: 58 LPGSA--------PVESAH--AIPGYRRVPTNEEGGFAFSISQP---QAPAGKPVA---Y 101 +A P +AH + G+ RVPT+ +G + S +P P G+P A Sbjct: 60 QADAAGIYAHAHDPRCAAHDPSFDGWGRVPTDAQGRYRLSTIRPGPVAGPNGQPQAAHLV 119 Query: 102 VTVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGA 161 V VF RGL++ T ++ DDP L+ +L VP +RR TL+AR + G Y+W+IHMQG Sbjct: 120 VLVFMRGLLRGVATRLYFADDPQLSGDPILALVPPERRGTLLARARSAGDYVWDIHMQGD 179 Query: 162 RETVFFDY 169 ETVFF Y Sbjct: 180 AETVFFSY 187 >Q4UZQ8.1 Q4UZQ8_XANC8 Protocatechuate 3,4-dioxygenase alpha chain Length = 187 Score = 110 bits (275), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 70/188 (37%), Positives = 103/188 (54%), Gaps = 25/188 (13%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVD-----SSAPQVKINGAILDGDGVPINDAWAEAW 57 + T SQT+GP+ + +E R ++ QV+I+G + DG G+P++DA E W Sbjct: 4 HATPSQTVGPY----YRLGLEPLYRAQIAPEHAAGTQVQISGCVFDGAGMPVSDAVLELW 59 Query: 58 LPGSA--------PVESAH--AIPGYRRVPTNEEGGFAFSISQP---QAPAGKPVA---Y 101 +A P +AH + G+ RVPT+ +G + S +P P G+P A Sbjct: 60 QADAAGIYAHAHDPRCAAHDPSFDGWGRVPTDAQGRYRLSTIRPGPVAGPNGQPQAAHLV 119 Query: 102 VTVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGA 161 V VF RGL++ T ++ DDP L+ +L VP +RR TL+AR + G Y+W+IHMQG Sbjct: 120 VLVFMRGLLRGVATRLYFADDPQLSGDPILALVPPERRGTLLARARSAGDYVWDIHMQGD 179 Query: 162 RETVFFDY 169 ETVFF Y Sbjct: 180 AETVFFSY 187 >B0RMP0.1 B0RMP0_XANCB Protocatechuate 3,4-dioxygenase alpha chain EC=1.13.11.3 Length = 187 Score = 110 bits (275), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 70/188 (37%), Positives = 103/188 (54%), Gaps = 25/188 (13%) Query: 3 NITTSQTIGPFPHEAWAWAVELTSRVD-----SSAPQVKINGAILDGDGVPINDAWAEAW 57 + T SQT+GP+ + +E R ++ QV+I+G + DG G+P++DA E W Sbjct: 4 HATPSQTVGPY----YRLGLEPLYRAQIAPEHAAGTQVQISGCVFDGAGMPVSDAVLELW 59 Query: 58 LPGSA--------PVESAH--AIPGYRRVPTNEEGGFAFSISQP---QAPAGKPVA---Y 101 +A P +AH + G+ RVPT+ +G + S +P P G+P A Sbjct: 60 QADAAGIYAHAHDPRCAAHDPSFDGWGRVPTDAQGRYRLSTIRPGPVAGPNGQPQAAHLV 119 Query: 102 VTVFARGLVKHQFTAVFLEDDPALAQSALLEQVPADRRDTLIARKQPDGSYLWNIHMQGA 161 V VF RGL++ T ++ DDP L+ +L VP +RR TL+AR + G Y+W+IHMQG Sbjct: 120 VLVFMRGLLRGVATRLYFADDPQLSGDPILALVPPERRGTLLARARSAGDYVWDIHMQGD 179 Query: 162 RETVFFDY 169 ETVFF Y Sbjct: 180 AETVFFSY 187