BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0121.p (193 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IVU0.1 D8IVU0_HERSS Small-conductance mechanosensitive channel... 385 e-105 B2UEB1.1 B2UEB1_RALPJ Small-conductance mechanosensitive channel... 258 4e-67 A9BNI5.1 A9BNI5_DELAS Small-conductance mechanosensitive channel... 192 2e-47 E8TYI5.1 E8TYI5_ALIDB MscS Mechanosensitive ion channel 191 4e-47 A1WAV0.1 A1WAV0_ACISJ Small-conductance mechanosensitive channel... 180 1e-43 B9MDN1.1 B9MDN1_ACIET Small-conductance mechanosensitive channel... 179 1e-43 E6WTB1.1 E6WTB1_PSEUU MscS Mechanosensitive ion channel 176 1e-42 D0J1J2.1 D0J1J2_COMT2 Putative uncharacterized protein 175 4e-42 D8D448.1 D8D448_COMTE Putative uncharacterized protein 175 4e-42 B7WS14.1 B7WS14_COMTE Small-conductance mechanosensitive channel... 174 5e-42 >D8IVU0.1 D8IVU0_HERSS Small-conductance mechanosensitive channel protein Length = 193 Score = 385 bits (989), Expect = e-105, Method: Compositional matrix adjust. Identities = 192/193 (99%), Positives = 193/193 (100%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA Sbjct: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI Sbjct: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR 180 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR Sbjct: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR 180 Query: 181 IVRRWSDGSGALF 193 IVRRWSDGSG+LF Sbjct: 181 IVRRWSDGSGSLF 193 >B2UEB1.1 B2UEB1_RALPJ Small-conductance mechanosensitive channel-like protein Length = 187 Score = 258 bits (658), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 125/187 (66%), Positives = 146/187 (78%), Gaps = 1/187 (0%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 M+K LQ+IH+ LPQWLADWFD+LVP EV+LI GA +R+AR L+ +L TY P K Sbjct: 1 MNKLLQAIHESLPQWLADWFDILVPAIEVVLIAAGAWLAVRIARRLLRKLTNTYSLPGKV 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AAL+ RV+ VY A LWALERMGVS LW+AFTGFA VGAVAFFAAWSVLSNLFCA+ Sbjct: 61 AALSLRVVATIVYVAATLWALERMGVSGTVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH-EGGETTLQLPNSLFFQ 179 LIY T FR+GDV+E+LE G+KPG+KGRVLD+NLVYTTL E EG E LQLPNSL FQ Sbjct: 121 LIYVTGAFRIGDVVELLENGEKPGIKGRVLDVNLVYTTLLETTVEGQEVVLQLPNSLVFQ 180 Query: 180 RIVRRWS 186 R++RRW Sbjct: 181 RVLRRWK 187 >A9BNI5.1 A9BNI5_DELAS Small-conductance mechanosensitive channel-like protein Length = 226 Score = 192 bits (488), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 95/192 (49%), Positives = 127/192 (66%), Gaps = 2/192 (1%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 M + ++ LP W+ DW + ++P A+++LI+ A + R+ R LI R YG P + Sbjct: 1 MFEVMRRFQAHLPTWMQDWLEFIIPGAQILLIIGAAWLLNRLVRRLIARAGRHYGLPPEL 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 + V + + + LER+GVS+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+ Sbjct: 61 LVPLRGVFRWILIAATVPLVLERLGVSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFF 178 LI PFR+GD IEVL+ +KPG KGRV+DIN++YTTL + H +G LQ+PNSL F Sbjct: 121 LIVIVGPFRLGDYIEVLDTAEKPGAKGRVIDINMLYTTLQDAHAPDGSPNLLQIPNSLIF 180 Query: 179 QRIVRRWSDGSG 190 QR+VRRW G G Sbjct: 181 QRVVRRWKGGLG 192 >E8TYI5.1 E8TYI5_ALIDB MscS Mechanosensitive ion channel Length = 220 Score = 191 bits (486), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 109/190 (57%), Positives = 132/190 (69%), Gaps = 3/190 (1%) Query: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 MS L+ + LP W DW DV+VP ++ LIVL AL + + R LI R + Y FP++ Sbjct: 1 MSPMLERLKTILPPWAHDWLDVIVPGMQIALIVLAALLLHYIVRRLIRRASDHYQFPHEL 60 Query: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 V+ + + GGA L ALER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA Sbjct: 61 LTPVNAVVRWLILGGAALLALERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAF 120 Query: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFF 178 LI T PFRVGD IE+L+ +KPG GRVLDINL+YTTL A E G T LQ+PN+L F Sbjct: 121 LILTVGPFRVGDHIELLDTAEKPGALGRVLDINLLYTTLEDATAPEPG-TLLQIPNALIF 179 Query: 179 QRIVRRWSDG 188 QR+VRRW G Sbjct: 180 QRVVRRWRAG 189 >A1WAV0.1 A1WAV0_ACISJ Small-conductance mechanosensitive channel-like protein Length = 222 Score = 180 bits (456), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 102/188 (54%), Positives = 130/188 (69%), Gaps = 3/188 (1%) Query: 4 FLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAAL 63 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 1 MLPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLTP 60 Query: 64 TQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIY 123 V+ + + G A L LER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA LI+ Sbjct: 61 INAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIF 120 Query: 124 TTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFFQRI 181 T PFRVGD IE+L+ DKPG GRV+DINL+Y TL A E G T LQ+PN+L FQR+ Sbjct: 121 TVGPFRVGDHIELLDTADKPGALGRVMDINLLYITLEDATAPEPG-TLLQIPNTLIFQRV 179 Query: 182 VRRWSDGS 189 VRRW G+ Sbjct: 180 VRRWRAGT 187 >B9MDN1.1 B9MDN1_ACIET Small-conductance mechanosensitive channel-like protein Length = 222 Score = 179 bits (455), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 102/188 (54%), Positives = 130/188 (69%), Gaps = 3/188 (1%) Query: 4 FLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAAL 63 L + LP W+ DW D++VP +++LIVL AL + R+ +I R + Y FP++ Sbjct: 1 MLPRLKTALPVWMHDWLDIIVPGVQIVLIVLAALLLQRLLLRIIMRASDHYQFPHELLIP 60 Query: 64 TQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIY 123 V+ + + G A L LER+GVS+A LW+AFTGFA VGAVAFFAAWSVLSNLFCA LI+ Sbjct: 61 INAVVRWLILGSAGLLVLERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAFLIF 120 Query: 124 TTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTL--AEEHEGGETTLQLPNSLFFQRI 181 T PFRVGD IE+L+ DKPG GRV+DINL+Y TL A E G T LQ+PN+L FQR+ Sbjct: 121 TVGPFRVGDHIELLDTADKPGALGRVMDINLLYITLEDATAPEPG-TLLQIPNTLIFQRV 179 Query: 182 VRRWSDGS 189 VRRW G+ Sbjct: 180 VRRWRAGT 187 >E6WTB1.1 E6WTB1_PSEUU MscS Mechanosensitive ion channel Length = 204 Score = 176 bits (446), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 102/183 (55%), Positives = 129/183 (70%), Gaps = 7/183 (3%) Query: 8 IHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALTQRV 67 + LP+WL W +VP + +LIVL A + ++ R L+ RL G P + + RV Sbjct: 1 MKHHLPEWLQGWLGTIVPIGQALLIVLAAWLLQQLVRRLVRRLMDRRGLPPEMGVVLHRV 60 Query: 68 LGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYTTQP 127 + GALL LER+GVS LW+AFT FAAVGAVAFFAAWSVLSN+FC +LI+TT+P Sbjct: 61 SAALIGFGALLMVLERLGVSGTVLWTAFTSFAAVGAVAFFAAWSVLSNIFCTVLIFTTRP 120 Query: 128 FRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAE-----EHEGGETTLQLPNSLFFQRIV 182 FR+ D IEV+E G+KPGLKGRV+DINL+YTTL E EH G ++LQ+PNSLFFQR+V Sbjct: 121 FRLYDYIEVVENGEKPGLKGRVVDINLIYTTLQEVPRDPEHAG--SSLQVPNSLFFQRVV 178 Query: 183 RRW 185 RRW Sbjct: 179 RRW 181 >D0J1J2.1 D0J1J2_COMT2 Putative uncharacterized protein Length = 218 Score = 175 bits (443), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 89/186 (47%), Positives = 125/186 (67%), Gaps = 2/186 (1%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 ++ + LP W+ DW ++L+P ++ LI+L A I + + +I R Y P++ Sbjct: 1 MRRLQMHLPDWMQDWLEILIPGVQIFLIILVAWLINWLIKRIISRAGKAYDLPHELLMPL 60 Query: 65 QRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYT 124 + V + + A+L LER+G+S+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI T Sbjct: 61 RGVFRWIIIAAAILAILERLGMSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVT 120 Query: 125 TQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIV 182 PFR+GD IEVL+ +KPG KGRV+D+N++Y TL ++ G LQ+PNSL FQR++ Sbjct: 121 VGPFRLGDYIEVLDTAEKPGAKGRVIDLNMLYITLQDDSAPAGMPNLLQIPNSLIFQRVI 180 Query: 183 RRWSDG 188 RRW G Sbjct: 181 RRWKGG 186 >D8D448.1 D8D448_COMTE Putative uncharacterized protein Length = 218 Score = 175 bits (443), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 89/186 (47%), Positives = 125/186 (67%), Gaps = 2/186 (1%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 ++ + LP W+ DW ++L+P ++ LI+L A I + + +I R Y P++ Sbjct: 1 MRRLQMHLPDWMQDWLEILIPGVQIFLIILVAWLINWLIKRIISRAGKAYDLPHELLMPL 60 Query: 65 QRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYT 124 + V + + A+L LER+G+S+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI T Sbjct: 61 RGVFRWIIIAAAILAILERLGMSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVT 120 Query: 125 TQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIV 182 PFR+GD IEVL+ +KPG KGRV+D+N++Y TL ++ G LQ+PNSL FQR++ Sbjct: 121 VGPFRLGDYIEVLDTAEKPGAKGRVIDLNMLYITLQDDSAPAGMPNLLQIPNSLIFQRVI 180 Query: 183 RRWSDG 188 RRW G Sbjct: 181 RRWKGG 186 >B7WS14.1 B7WS14_COMTE Small-conductance mechanosensitive channel-like protein Length = 218 Score = 174 bits (441), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 89/186 (47%), Positives = 125/186 (67%), Gaps = 2/186 (1%) Query: 5 LQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKAAALT 64 ++ + LP W+ DW ++L+P ++ LI+L A I + + +I R Y P++ Sbjct: 1 MRRLQMHLPDWMQDWLEILIPGIQIFLIILVAWLINWLIKRIISRAGKAYDLPHELLMPL 60 Query: 65 QRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYT 124 + V + + A+L LER+G+S+ LW+AFTGFA VGAVAFFAAWSVLSNLFCA+LI T Sbjct: 61 RGVFRWIIIAAAVLAILERLGMSATVLWTAFTGFATVGAVAFFAAWSVLSNLFCALLIVT 120 Query: 125 TQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEH--EGGETTLQLPNSLFFQRIV 182 PFR+GD IEVL+ +KPG KGRV+D+N++Y TL ++ G LQ+PNSL FQR++ Sbjct: 121 VGPFRLGDYIEVLDTAEKPGAKGRVIDLNMLYITLQDDSAPAGMPNLLQIPNSLIFQRVI 180 Query: 183 RRWSDG 188 RRW G Sbjct: 181 RRWKGG 186