BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0001.p (247 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J248.1 D8J248_HERSS 2-keto-4-pentenoate hydratase protein EC=4... 478 e-133 B0UH43.1 B0UH43_METS4 Hydratase/decarboxylase 196 2e-48 C5CW75.1 C5CW75_VARPS 2-oxopent-4-enoate hydratase 176 4e-42 Q0K2U8.1 Q0K2U8_CUPNH 2-Oxopent-4-enoate hydratase EC=4.2.1.80 174 8e-42 B8ES83.1 B8ES83_METSB Fumarylacetoacetate (FAA) hydrolase 127 1e-27 D6UUS2.1 D6UUS2_9BACT Putative uncharacterized protein 119 3e-25 D5QFA2.1 D5QFA2_GLUHA Putative 2-keto-4-pentenoate hydratase-lik... 119 6e-25 B2JQ34.1 B2JQ34_BURP8 2-keto-4-pentenoate hydratase-like protein 112 4e-23 B2IFR2.1 B2IFR2_BEII9 Putative 2-keto-4-pentenoate hydratase-lik... 112 6e-23 E8YM69.1 E8YM69_9BURK Fumarylacetoacetate (FAA) hydrolase 111 8e-23 >D8J248.1 D8J248_HERSS 2-keto-4-pentenoate hydratase protein EC=4.2.1.80 Length = 247 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 243/247 (98%), Positives = 244/247 (98%) Query: 1 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT 60 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT Sbjct: 1 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT 60 Query: 61 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL 120 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL Sbjct: 61 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL 120 Query: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQVS 180 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPD+LSLRQIEASLSIDGQQVS Sbjct: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDLLSLRQIEASLSIDGQQVS 180 Query: 181 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGA 240 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCT MARGGRRVEAALHGIGA Sbjct: 181 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCTGLQMARGGRRVEAALHGIGA 240 Query: 241 VWLDFAG 247 VWLDFAG Sbjct: 241 VWLDFAG 247 >B0UH43.1 B0UH43_METS4 Hydratase/decarboxylase Length = 238 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 127/241 (52%), Positives = 151/241 (62%), Gaps = 6/241 (2%) Query: 7 TQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPL 66 LLL AR G + WR +A D AYAVQDA RLGPVGGWKVG+ P AP Sbjct: 2 ADLLLAAR-GGARVPWRAVAPADPAGAYAVQDAVAARLGPVGGWKVGAANPEAVPNAAPC 60 Query: 67 PASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVV 126 PA+ + SG L GP+WR+RG+E E+ LR+GRD D AEL +A+LP IEVV Sbjct: 61 PAACLRGSGAVLDGPEWRLRGIEAEVGLRLGRDLDGAAP---RAELAAAVEAVLPVIEVV 117 Query: 127 ETRLGHPPCTNPWAGMADLQSHGALVTGAPQAL-PDVLSLRQIEASLSIDGQQVSHTTGG 185 ETRL +P A +ADLQSHGAL+ G P L P L LR + A LS+DG+ V+ T GG Sbjct: 118 ETRLDGWGEADPLARLADLQSHGALILGPPSRLDPAALDLRTVAADLSVDGRAVAATRGG 177 Query: 186 NPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAVWLD 244 NPA D+W LL WLA H A RG+P RAGQ++TTGSCT G RV A L GIGAV + Sbjct: 178 NPAEDVWRLLAWLADHAAARGRPLRAGQVVTTGSCTGMLFCAQGTRVAAELAGIGAVAMR 237 Query: 245 F 245 F Sbjct: 238 F 238 >C5CW75.1 C5CW75_VARPS 2-oxopent-4-enoate hydratase Length = 254 Score = 176 bits (445), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 102/215 (47%), Positives = 130/215 (60%), Gaps = 2/215 (0%) Query: 33 AYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEVEL 92 AYA+QDA L +GPVGGWKVG++ G P CAPLPA G+LP G L GP WR+RG+E E+ Sbjct: 40 AYAIQDATLAAIGPVGGWKVGARAPGQEPTCAPLPAEGLLPGGVRLQGPAWRLRGIEAEV 99 Query: 93 ALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALV 152 ++G D P A++ +++LP IEVVETRL + A +ADL SHGALV Sbjct: 100 GFQLGADLPPRAAPYTLADVAGAVESVLPVIEVVETRLADWFGAHANALLADLLSHGALV 159 Query: 153 TGAPQAL-PDVLSLRQIEASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRA 210 G Q P L++ EA+L DGQ V+HT G +P+ D LL WLA HCA RG +A Sbjct: 160 LGQRQPFAPAWFDLQRAEAALHFDGQTVAHTVGEHPSPDAGALLVWLANHCAARGAGLKA 219 Query: 211 GQIITTGSCTCHTMARGGRRVEAALHGIGAVWLDF 245 GQI+T+GSCT A G V + + V L F Sbjct: 220 GQIVTSGSCTGMLFASAGTAVRVEIGELAPVALSF 254 >Q0K2U8.1 Q0K2U8_CUPNH 2-Oxopent-4-enoate hydratase EC=4.2.1.80 Length = 254 Score = 174 bits (442), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 109/243 (44%), Positives = 144/243 (59%), Gaps = 4/243 (1%) Query: 5 AATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCA 64 A Q+L AR G +D L +YAVQDA L +GP+GGWKVG++G G P CA Sbjct: 12 ALAQMLCVARTRGERVDPALLHPLAHGGSYAVQDATLAMIGPIGGWKVGARGPGQEPACA 71 Query: 65 PLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIE 124 PLPA+G+LP G L GP WR+RG+EVE+A +G P + + ++ ++PAIE Sbjct: 72 PLPAAGLLPDGVGLRGPAWRLRGIEVEVAFVLGASLPPRGRPYERRDIEAALALVVPAIE 131 Query: 125 VVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALP-DVLSLRQIEASLSIDGQQVSHTT 183 VVE+RL A +ADL SHGALVTG P + L L +EA+L + V+HT Sbjct: 132 VVESRLEDWLGAGSDAQLADLLSHGALVTGRPTPFAREWLHLGALEATLRFGRRTVAHTV 191 Query: 184 GGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIG--A 240 GG+ + D+ LL WLA HCA RG +AGQ++TTG+CT A G RV A + G+G A Sbjct: 192 GGHQSPDVGALLVWLANHCAARGAGLKAGQVVTTGTCTGLLFASAGTRVSAGIEGLGTLA 251 Query: 241 VWL 243 W Sbjct: 252 AWF 254 >B8ES83.1 B8ES83_METSB Fumarylacetoacetate (FAA) hydrolase Length = 260 Score = 127 bits (320), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 90/250 (36%), Positives = 122/250 (48%), Gaps = 12/250 (4%) Query: 1 MHIDAATQL---LLDARHSG-----LALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKV 52 M D AT L L+DA+ +G L ++ R + E AY VQ A RLGP+ GWKV Sbjct: 8 MECDEATALAALLIDAQETGASIGELPVNAR---ISSREEAYRVQSALARRLGPIVGWKV 64 Query: 53 GSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAEL 112 G K APL A+ PSG +R+ LE ELA R P + E+ Sbjct: 65 GRKAADAPASRAPLFANRTHPSGAKFERDAFRLFLLEAELAFRFRSGLPPIGRPYARDEV 124 Query: 113 RTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASL 172 T ++ A E++++R P P +ADL SHGA+V G+ +PD S SL Sbjct: 125 LTAISEVVVAFEIIDSRFRDWPTVAPELLLADLLSHGAMVVGSGVPMPDHPSFINTSVSL 184 Query: 173 SIDGQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRV 231 ++D + + GGNPA D+ L+ WLA G RAG ++TTGS TC G Sbjct: 185 AVDDRMIISRDGGNPAGDVIDLVVWLANDLLDVGAALRAGDLVTTGSYTCMERLAPGSSA 244 Query: 232 EAALHGIGAV 241 EA+ G+G V Sbjct: 245 EASFAGVGTV 254 >D6UUS2.1 D6UUS2_9BACT Putative uncharacterized protein Length = 254 Score = 119 bits (298), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 88/248 (35%), Positives = 118/248 (47%), Gaps = 4/248 (1%) Query: 2 HIDAATQLLLDARHSG--LALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGG 59 ++AA LLLDAR + +A L + + YAVQD G +GGWK+G+ Sbjct: 8 QLEAAANLLLDARRTNTPIADLPEELRPTNLDEVYAVQDMIAAAYGEIGGWKIGAPNPEA 67 Query: 60 TPGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAI 119 TP AP+PA V SG+ + G +W RGLE E+A VG D P E + Sbjct: 68 TPMYAPMPAQWVATSGSVIDGDRW-YRGLEAEIAFLVGEDLPPRATPYSREEAVAAMASC 126 Query: 120 LPAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQV 179 P IEV+E L + + +ADLQ HG + G + + +L++DG Sbjct: 127 HPVIEVLEPGLSDSTKADRNSTLADLQMHGGFIYGPAFDNWQSVDFNTEKVTLAVDGVIR 186 Query: 180 SHTTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGI 238 TG N + DL LL WLA A R R GQ ITTGS T +T A G + + Sbjct: 187 VERTGSNTSGDLVKLLPWLANEGAARTGGLRKGQWITTGSWTGNTQASAGSAADVVFSTV 246 Query: 239 GAVWLDFA 246 G+V L FA Sbjct: 247 GSVSLRFA 254 >D5QFA2.1 D5QFA2_GLUHA Putative 2-keto-4-pentenoate hydratase-like protein Length = 253 Score = 119 bits (297), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 81/217 (37%), Positives = 107/217 (49%), Gaps = 11/217 (5%) Query: 31 ETAYAVQDAQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQWRMRGLEV 90 E AY +QD LGP+ GWKVG++ P CAP+ AS + G T+ R G+E Sbjct: 37 EDAYRIQDEVAQHLGPIVGWKVGAQTPSSEPFCAPIHASTLFNDGATVPHGLCRHLGVEA 96 Query: 91 ELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGA 150 E+A R + P E+ E+ I P IE+++TR P + + AD QSHGA Sbjct: 97 EIAYRFAQALPPRERDWTRDEVMDRIGTIHPVIEILDTRFETPGSQHKFLHTADQQSHGA 156 Query: 151 LVTGAPQALPDVLSLRQIE-----ASLSIDGQQVSHTTGGNPA-DLWPLLGWLALHCARR 204 L+ G P V R+ LSI+ + V+ GGN A D LL WLA H +RR Sbjct: 157 LIVG-----PGVAEWRKYNPVVEHVRLSINDETVAEHVGGNSAGDPLRLLVWLANHASRR 211 Query: 205 GQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAV 241 AG I+TTGS T G RV A+ IG+V Sbjct: 212 NLGIDAGSIVTTGSTTGTIFVDHGARVSASFPAIGSV 248 >B2JQ34.1 B2JQ34_BURP8 2-keto-4-pentenoate hydratase-like protein Length = 257 Score = 112 bits (280), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 81/239 (33%), Positives = 111/239 (46%), Gaps = 5/239 (2%) Query: 8 QLLLDAR--HSGLALDWRRLAVGDAETAYAVQDAQLLRLGP-VGGWKVGSKGDGGTPGCA 64 Q L DAR H+ LA L D AYA+Q A L G +GGWK+G+K G A Sbjct: 14 QQLADARADHTTLASLPPELIPADLSAAYAIQHALLATRGARIGGWKIGAKSADGAIQGA 73 Query: 65 PLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIE 124 PLPA+ + G L ++ GLE+E+A R GR ++P E E+ +++ IE Sbjct: 74 PLPATDLHADGARLPREHYKPLGLELEVAFRFGRSFEPAANAYHEVEVLDAIESMAATIE 133 Query: 125 VVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQVSHTTG 184 +V +R P + A +ADLQ+HGAL+ G S S +GQ V T Sbjct: 134 IVASRYAQWPNVDKLAQLADLQNHGALIVGEFTHYRRDFPFVSPALSFSFEGQDVVKATP 193 Query: 185 GNPA-DLWPLLGWLALHCARRGQ-PWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAV 241 NP D LL WL HC + + I+TTGS T + + G+G V Sbjct: 194 SNPCGDPRRLLTWLVNHCTQHHRVAVTPDMIVTTGSYTGMFFPQHAGTARGHIEGLGPV 252 >B2IFR2.1 B2IFR2_BEII9 Putative 2-keto-4-pentenoate hydratase-like protein Length = 253 Score = 112 bits (279), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 84/227 (37%), Positives = 106/227 (46%), Gaps = 11/227 (4%) Query: 28 GDAETAYAVQD----AQLLRLGPVGGWKVGSKGDGGTPGCAPLPASGVLPSGTTLAGPQ- 82 + E AY VQ A + R GP+ WKVGS P AP+ A+ V TT+ GP Sbjct: 30 SNEEAAYDVQQQVTTALIKRDGPIIAWKVGSATPTAEPFAAPIHAATVF---TTMPGPSP 86 Query: 83 --WRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWA 140 +R G+E ELA R R + AI IE+V+TR P P Sbjct: 87 SFFRYIGVEAELAYRFDRTPIVTSDGCTNEAITEALGAIHAVIEIVDTRFSKPGIAEPLV 146 Query: 141 GMADLQSHGALVTGAPQALPDVLSLRQIEASLSIDGQQVSHTTGGNPA-DLWPLLGWLAL 199 AD Q HGAL+ G + +S Q L I+G+ + GGN A D LL WLA+ Sbjct: 147 HRADQQGHGALIIGPGRRDWQAVSPLQERVKLMINGRTAADHEGGNSAGDPMRLLVWLAV 206 Query: 200 HCARRGQPWRAGQIITTGSCTCHTMARGGRRVEAALHGIGAVWLDFA 246 H A+RG P AG I+TTGS T RVEA IG + LD + Sbjct: 207 HAAQRGMPLTAGTIVTTGSTTGTIWVAANTRVEAEFASIGRLTLDLS 253 >E8YM69.1 E8YM69_9BURK Fumarylacetoacetate (FAA) hydrolase Length = 253 Score = 111 bits (278), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 85/244 (34%), Positives = 119/244 (48%), Gaps = 9/244 (3%) Query: 10 LLDARHSGLALDW--RRLAVGDAETAYAVQDAQLLRLG-PVGGWKVGSKGDGGTPGCAPL 66 L++AR +D L DA TAYA+Q A + L G WK+G+K GG AP+ Sbjct: 10 LVEARRQHRPIDGLPSELMPPDAATAYAIQQAVITGLADTTGAWKIGAKAPGGPTSGAPI 69 Query: 67 PASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAILPAIEVV 126 PAS VLPS +A + +E+E+A R +P + E+ + +LPAIE+V Sbjct: 70 PASLVLPSPARVAHGDFFRVLVELEIAFRFAEAIEPRSRAYTRDEVLGLVGVVLPAIEIV 129 Query: 127 ETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDV---LSLRQIEASLSIDGQQVSHTT 183 ++R P P A +AD Q++GAL+TG P A + E LS DGQ + Sbjct: 130 DSRFAEWPNVAPLAQLADAQNNGALITGHPVAYAGLARGFDFVSPELELSFDGQSLVPDA 189 Query: 184 GGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCT-CHTMARGGRRVEAALHGIGAV 241 GNPA D LL W HCA G +TTGS H + + G V + G+G V Sbjct: 190 TGNPAGDPRELLVWFVNHCAAMGITIEPEWTLTTGSYVGAHKVGKPG-IVRGHIDGLGEV 248 Query: 242 WLDF 245 ++ Sbjct: 249 EIEL 252