BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0045.p (219 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8J0B1.1 D8J0B1_HERSS Nitrile hydratase subunit beta protein EC=... 452 e-125 P27763.1 NHAB_PSECL Nitrile hydratase subunit beta EC=4.2.1.84 364 5e-99 F0EAG9.1 F0EAG9_9PSED Nitrile hydratase 362 1e-98 C3KC40.1 C3KC40_PSEFS Putative low-molecular weight cobalt-conta... 362 1e-98 E2XQ67.1 E2XQ67_PSEFL Nitrile hydratase subunit beta 353 8e-96 A5W403.1 A5W403_PSEP1 Nitrile hydratase EC=4.2.1.84 353 1e-95 E4RFH7.1 E4RFH7_PSEPB Nitrile hydratase EC=4.2.1.84 352 2e-95 F0QD97.1 F0QD97_ACIAP Nitrile hydratase EC=4.2.1.84 347 1e-93 F2LLG1.1 F2LLG1_BURGA Nitrile hydratase 346 1e-93 Q0B186.1 Q0B186_BURCM Nitrile hydratase EC=4.2.1.84 343 7e-93 >D8J0B1.1 D8J0B1_HERSS Nitrile hydratase subunit beta protein EC=4.2.1.84 Length = 219 Score = 452 bits (1163), Expect = e-125, Method: Compositional matrix adjust. Identities = 218/219 (99%), Positives = 218/219 (99%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV Sbjct: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG Sbjct: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT Sbjct: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKAPQQGGRA 219 YHVQF NRDVWGDACDDGFVVVDLFEGYLDKAPQQGGRA Sbjct: 181 YHVQFSNRDVWGDACDDGFVVVDLFEGYLDKAPQQGGRA 219 >P27763.1 NHAB_PSECL Nitrile hydratase subunit beta EC=4.2.1.84 Length = 220 Score = 364 bits (934), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 168/213 (78%), Positives = 189/213 (88%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PHTINSL YK+VFKQDWEHLAY+LMF+G D +K +SVDEVRHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHTINSLSYKQVFKQDWEHLAYSLMFVGVDQLKKFSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ER++ RQ TQYYERY+IATA+LLVE GV+TQ ELD+ALGSHF+LA PA + GRPAITG Sbjct: 61 ERLDVRQHVGTQYYERYIIATATLLVETGVITQAELDQALGSHFKLANPAHATGRPAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 RPPFEVGDRV+VRDE V GHIR P YVRGKEGVVLHRT+EQWPFPD IGHGD SAA QPT Sbjct: 121 RPPFEVGDRVVVRDEYVAGHIRMPAYVRGKEGVVLHRTSEQWPFPDAIGHGDLSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKAP 213 YHV+FR +D+WGDA DDG+VVVDLFE YLDKAP Sbjct: 181 YHVEFRVKDLWGDAADDGYVVVDLFESYLDKAP 213 >F0EAG9.1 F0EAG9_9PSED Nitrile hydratase Length = 220 Score = 362 bits (930), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 170/217 (78%), Positives = 189/217 (87%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PH INSL YK+VFKQDWEHLAY+LMF+GADHMK +SVDE+RHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHRINSLSYKQVFKQDWEHLAYSLMFIGADHMKKFSVDEIRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIATA+LLVE GV+TQ ELDEALGSHF+LA PA +KGR AITG Sbjct: 61 ERIDVRQHVGTEYYERYVIATATLLVETGVITQAELDEALGSHFKLANPAHAKGRAAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 R PFEVGDRV+VRDE V GH+R PGYVRGK+GVVLHRTTEQWPFPD IGHGD+SAA QPT Sbjct: 121 RAPFEVGDRVIVRDEYVAGHVRMPGYVRGKQGVVLHRTTEQWPFPDAIGHGDQSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKAPQQGG 217 YHV+FR RD+WGDA DDG VVVDLFE YLDK G Sbjct: 181 YHVEFRVRDLWGDAADDGRVVVDLFESYLDKVEGSGA 217 >C3KC40.1 C3KC40_PSEFS Putative low-molecular weight cobalt-containing nitrile hydratase subunit beta EC=4.2.1.84 Length = 220 Score = 362 bits (930), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 166/212 (78%), Positives = 190/212 (89%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PHT+NSL Y+ VFKQDWEHLAY+LMF+GADH+K +SVDEVRHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHTVNSLSYQPVFKQDWEHLAYSLMFIGADHLKKFSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ER++ RQ TQYYERYVIATA+LLVE GV+TQ ELD+ALG+HF+LA PA ++GRPAITG Sbjct: 61 ERLDVRQHVGTQYYERYVIATATLLVETGVITQAELDQALGAHFKLANPAHAQGRPAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 RPPFEVGDRV+VRDE V GH+R P YVRGKEGVVLHRT+E+WPFPD IGHGD SAA QPT Sbjct: 121 RPPFEVGDRVVVRDEYVAGHVRMPAYVRGKEGVVLHRTSEKWPFPDAIGHGDLSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKA 212 YHV+FR +D+WGDA DDGFVVVDLFE YLDKA Sbjct: 181 YHVEFRVKDLWGDAADDGFVVVDLFESYLDKA 212 >E2XQ67.1 E2XQ67_PSEFL Nitrile hydratase subunit beta Length = 218 Score = 353 bits (907), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 163/212 (76%), Positives = 185/212 (87%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PHTINSL Y+ VFKQDWEHLAY+LMF+GADH+K +SVDEVRHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHTINSLSYQPVFKQDWEHLAYSLMFIGADHLKKFSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ER++ RQ TQYYERYVIATA+LLVE GV+TQ ELD+ALGSHF+LA PA + GRPAITG Sbjct: 61 ERLDVRQHVGTQYYERYVIATATLLVETGVITQAELDQALGSHFKLANPAHANGRPAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 R PFEVGDRV+VRDE V GH+R P YVRGK GVVLHRT++ WPFPD IGHGD SAA QPT Sbjct: 121 RAPFEVGDRVVVRDEYVAGHVRMPAYVRGKHGVVLHRTSQTWPFPDAIGHGDTSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKA 212 YHV+FR +D+WGDA D+GFVVVDLFE YLD A Sbjct: 181 YHVEFRVKDLWGDAADEGFVVVDLFESYLDHA 212 >A5W403.1 A5W403_PSEP1 Nitrile hydratase EC=4.2.1.84 Length = 220 Score = 353 bits (905), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 162/211 (76%), Positives = 184/211 (87%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PH INSL YK+VFKQDWEHLAY+LMF+G DH+ +SVDE+RHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHRINSLSYKQVFKQDWEHLAYSLMFIGVDHLNKFSVDEIRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIATA+LLVE GV+TQ ELDE LGSHF+LA PA ++GR AITG Sbjct: 61 ERIDVRQHVGTEYYERYVIATATLLVETGVITQAELDETLGSHFKLANPAHAQGRAAITG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 R PFEVGDRV+VRDE V GH+R P YVRGK+GVVLHRTTEQWPFPD IGHGD+SAA QPT Sbjct: 121 RAPFEVGDRVIVRDEYVAGHVRMPAYVRGKQGVVLHRTTEQWPFPDAIGHGDQSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDK 211 YHV+FR RD+WGDA DDG VVVDLFE YLD+ Sbjct: 181 YHVEFRVRDLWGDAADDGLVVVDLFESYLDR 211 >E4RFH7.1 E4RFH7_PSEPB Nitrile hydratase EC=4.2.1.84 Length = 220 Score = 352 bits (903), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 162/211 (76%), Positives = 184/211 (87%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PH INSL YK+VFKQDWEHLAY+LMF+G DH+ +SVDE+RHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHRINSLSYKQVFKQDWEHLAYSLMFIGVDHLNKFSVDEIRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIATA+LLVE GV+TQ ELDEALGSHF+LA PA ++GR AI G Sbjct: 61 ERIDVRQHVGTEYYERYVIATATLLVETGVITQAELDEALGSHFKLANPAHAQGRAAIIG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 R PFEVGDRV+VRDE V GH+R P YVRGK+GVVLHRTTEQWPFPD IGHGD+SAA QPT Sbjct: 121 RAPFEVGDRVIVRDEYVAGHVRMPAYVRGKQGVVLHRTTEQWPFPDAIGHGDQSAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDK 211 YHV+FR RD+WGDA DDG VVVDLFE YLD+ Sbjct: 181 YHVEFRVRDLWGDAADDGLVVVDLFESYLDR 211 >F0QD97.1 F0QD97_ACIAP Nitrile hydratase EC=4.2.1.84 Length = 233 Score = 347 bits (889), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 162/211 (76%), Positives = 181/211 (85%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG++PHTINSLDY+KVFK+DWEHL Y L+FLGA H++ +S+DEVRHAV Sbjct: 1 MDGFHDLGGFQGFGKVPHTINSLDYQKVFKEDWEHLGYCLLFLGAAHLQKFSIDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIA A+LLVE GVLTQQELDEALGS F+LA PA S GR A+TG Sbjct: 61 ERIDVRQHVGTRYYERYVIAVATLLVETGVLTQQELDEALGSPFKLANPAHSPGRAAVTG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 R PFEVGDRV+VRDE +GHIRCP YVRGK+GVVLHRTTE+WPFPD IGHGD SA QPT Sbjct: 121 RAPFEVGDRVVVRDEYFSGHIRCPAYVRGKQGVVLHRTTEKWPFPDAIGHGDASAEHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDK 211 YHVQF RD+WG A DDG VVVDLFE YLDK Sbjct: 181 YHVQFSVRDLWGSAADDGLVVVDLFESYLDK 211 >F2LLG1.1 F2LLG1_BURGA Nitrile hydratase Length = 230 Score = 346 bits (888), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 162/215 (75%), Positives = 183/215 (85%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFG +PHTINSL YK VFK+DWEHLAY+LMF+G DH+K +SVDEVRHAV Sbjct: 1 MDGFHDLGGFQGFGPVPHTINSLSYKPVFKEDWEHLAYSLMFVGVDHLKKFSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIATA+LLVE GVLTQ ELD ALGSHF+LA P+ S GRPA G Sbjct: 61 ERIDVRQHVGTRYYERYVIATATLLVETGVLTQAELDAALGSHFKLANPSHSTGRPAPVG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 FEVG+RV+VRDE V+GHIR PGYVRGKEGVVLHRTTE WPFPDTIGHGD A QPT Sbjct: 121 GRHFEVGERVIVRDEYVSGHIRAPGYVRGKEGVVLHRTTESWPFPDTIGHGDTRAIHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKAPQQ 215 YHV+FR +D+WGDA DDG+VV DLFEGYL++AP + Sbjct: 181 YHVEFRTKDLWGDAADDGYVVADLFEGYLNRAPAR 215 >Q0B186.1 Q0B186_BURCM Nitrile hydratase EC=4.2.1.84 Length = 217 Score = 343 bits (881), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 163/214 (76%), Positives = 181/214 (84%) Query: 1 MDGFHDLGGVQGFGRIPHTINSLDYKKVFKQDWEHLAYTLMFLGADHMKLYSVDEVRHAV 60 MDGFHDLGG QGFGR+PHTINSL YK VFK+DWEHLAY+LMF+G D +K +SVDEVRHAV Sbjct: 1 MDGFHDLGGFQGFGRVPHTINSLGYKPVFKEDWEHLAYSLMFVGVDQLKKFSVDEVRHAV 60 Query: 61 ERIEFRQQANTQYYERYVIATASLLVELGVLTQQELDEALGSHFQLARPAQSKGRPAITG 120 ERI+ RQ T+YYERYVIATA+LLVE GVLTQ ELD ALGSHF+LA P+ S GR A G Sbjct: 61 ERIDVRQHVGTRYYERYVIATATLLVETGVLTQDELDRALGSHFRLANPSHSVGRAAPAG 120 Query: 121 RPPFEVGDRVLVRDERVTGHIRCPGYVRGKEGVVLHRTTEQWPFPDTIGHGDKSAAMQPT 180 FEVGDRV+VRDE V+GHIR PGYVRGK GVVLHRTTE WPFPD IGHGD AA QPT Sbjct: 121 ATRFEVGDRVVVRDEYVSGHIRAPGYVRGKTGVVLHRTTESWPFPDAIGHGDTGAAHQPT 180 Query: 181 YHVQFRNRDVWGDACDDGFVVVDLFEGYLDKAPQ 214 YHV+FR RD+WGDA DDG+VV DLFEGYLD+AP+ Sbjct: 181 YHVEFRVRDLWGDAADDGYVVADLFEGYLDRAPE 214