BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0244.p (446 letters) Database: pfamseq 15,929,002 sequences; 5,169,768,107 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value D8IZC2.1 D8IZC2_HERSS tRNA modifying 2-methylthioadenine synthet... 918 0.0 A6SV24.1 MIAB_JANMA (Dimethylallyl)adenosine tRNA methylthiotran... 754 0.0 A4G267.1 MIAB_HERAR (Dimethylallyl)adenosine tRNA methylthiotran... 753 0.0 F2LAK3.1 F2LAK3_BURGA 2-methylthioadenine synthetase 701 0.0 B2JD88.1 MIAB_BURP8 (Dimethylallyl)adenosine tRNA methylthiotran... 696 0.0 F0G6S1.1 F0G6S1_9BURK (Dimethylallyl)adenosine tRNA methylthiotr... 696 0.0 B4EBG2.1 MIAB_BURCJ (Dimethylallyl)adenosine tRNA methylthiotran... 694 0.0 B1YWB9.1 MIAB_BURA4 (Dimethylallyl)adenosine tRNA methylthiotran... 693 0.0 Q0BC21.1 MIAB_BURCM (Dimethylallyl)adenosine tRNA methylthiotran... 693 0.0 B1FDJ2.1 B1FDJ2_9BURK tRNA-i(6)A37 thiotransferase enzyme MiaB E... 693 0.0 >D8IZC2.1 D8IZC2_HERSS tRNA modifying 2-methylthioadenine synthetase protein Length = 446 Score = 918 bits (2373), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/446 (100%), Positives = 446/446 (100%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF Sbjct: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY Sbjct: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH Sbjct: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQIIEVSITEAFPFSLRGEIVTQH Sbjct: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 >A6SV24.1 MIAB_JANMA (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 446 Score = 754 bits (1947), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/446 (79%), Positives = 401/446 (89%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKM DVLNAS+GLIKTD PE+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK NNP L+IGVGGCVASQEG+AI+KRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VL EVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAEL GIERIRFVTSH Sbjct: 181 VLAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLID YAKVPKL NHLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R+VRP+I Sbjct: 241 PKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 ++ SDFIVGFPGET+ DFEA+MKL+ D+G+DNSFSFIFS RPGTPAANL DDTP EVKL Sbjct: 301 SISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQ 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N R S VGTVQR+LVEG SK+ ELQGRTE+NRVVNF G +G R Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGAR 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQ+++V+I ++F ++LRGEI+ + Sbjct: 421 LIGQMVDVNIVQSFAYTLRGEIIVKQ 446 >A4G267.1 MIAB_HERAR (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 446 Score = 753 bits (1943), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/446 (79%), Positives = 400/446 (89%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKMADVLNASDGLIKTD+ E+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMADVLNASDGLIKTDKAEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK P L+IGVGGCVASQEG+AIIKRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKLLKPDLMIGVGGCVASQEGDAIIKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRF+D Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAE+ GIERIR+VTSH Sbjct: 181 VLTEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAEMEGIERIRYVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R VRP+I Sbjct: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLRAVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 ++ SDFIVGFPGET+EDFEA+MKL+ D+G+DNSFSFIFS RPGTPAANL DDTP EVKL Sbjct: 301 SISSDFIVGFPGETDEDFEAMMKLIDDIGYDNSFSFIFSPRPGTPAANLADDTPHEVKLK 360 Query: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N R S VG+VQR+LVEG SK+ ELQGRTE+NRVVNF GPN Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGSVQRILVEGPSKKDADELQGRTENNRVVNFSAGPNAAH 420 Query: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 LIGQ++ V+IT++ ++LRGEIV + Sbjct: 421 LIGQMVNVNITQSHAYTLRGEIVVKQ 446 >F2LAK3.1 F2LAK3_BURGA 2-methylthioadenine synthetase Length = 457 Score = 701 bits (1808), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/449 (73%), Positives = 385/449 (85%), Gaps = 3/449 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KK++IKTFGCQMNEYDSDKM DVLNAS+GL+KTDRPE+ADVIL NTCS+REKAQEKVF Sbjct: 1 MTKKIYIKTFGCQMNEYDSDKMVDVLNASEGLVKTDRPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ NP L++GVGGCVASQEG AI+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEANPNLIVGVGGCVASQEGAAIVARAPYVDVVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTE+AGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+AELPGIERIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGALTQGSEEIADFATLIEYVAELPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEFSQRL+D YA+VPKL NHL+LP QHGSDR L AMKRGYT LEYKS+IR++R +RP Sbjct: 241 SHPKEFSQRLLDVYARVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D I+GFPGETE DF+ M+LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDIIIGFPGETEADFDKTMQLVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARISA+ VG ++R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISASMVGRIERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIVTQH 446 RLIGQ+I+V I A+P SLRGE+V H Sbjct: 421 HARLIGQMIDVRINHAYPHSLRGELVLAH 449 >B2JD88.1 MIAB_BURP8 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 456 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/449 (72%), Positives = 384/449 (85%), Gaps = 3/449 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVL A++GL+KTD PE+ADVIL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ NP LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEANPNLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGRPQVDI+FPEIEKFDHLPPAR++G +A+VSIMEGCSKYCSYCVVPYTRGEEVSR +D Sbjct: 121 SGRPQVDITFPEIEKFDHLPPARIDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTE+AGLADQGV+E+ LLGQNVNAYRG M G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGAMTLGATEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M+LV D+ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMQLVHDMSYDTSFSFIYSPRPGTPAANLQDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNG 418 L RLQ LQA + +N RIS A VG V+R+LVE +++ +EL GRTE+NRVVNF Sbjct: 361 LRRLQHLQATIEENVQRISQAMVGKVERILVERPARKDPNELAGRTENNRVVNFPAPVET 420 Query: 419 Q-RLIGQIIEVSITEAFPFSLRGEIVTQH 446 RLIGQ+I+V I A+P SLRGE+V H Sbjct: 421 HARLIGQMIDVKIVHAYPHSLRGELVMVH 449 >F0G6S1.1 F0G6S1_9BURK (Dimethylallyl)adenosine tRNA methylthiotransferase Length = 457 Score = 696 bits (1796), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/446 (73%), Positives = 382/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDI+FPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL NHL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV D+ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHDMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE+V Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELV 446 >B4EBG2.1 MIAB_BURCJ (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 457 Score = 694 bits (1790), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDI+FPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL NHL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETE+DF+ +M LV ++G+D SFSFI+S RPGTPAANL DDTP++VK Sbjct: 301 DLSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPRDVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE+V Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELV 446 >B1YWB9.1 MIAB_BURA4 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 457 Score = 693 bits (1789), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV+IKTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYIKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE++ Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELL 446 >Q0BC21.1 MIAB_BURCM (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 457 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE++ Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELL 446 >B1FDJ2.1 B1FDJ2_9BURK tRNA-i(6)A37 thiotransferase enzyme MiaB EC=1.3.1.74 Length = 457 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/446 (73%), Positives = 383/446 (85%), Gaps = 3/446 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 M KKV++KTFGCQMNEYDSDKM DVLNA++GL KTD PE+AD+IL NTCS+REKAQEKVF Sbjct: 1 MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P LLIGVGGCVASQEG +I+ RAP+VD+VFGPQTLHRLPQMI +RR Sbjct: 61 SDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTEVAGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+A++PGIERIR+ T Sbjct: 181 VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEF+QRLIDTYAKVPKL +HL+LP QHGSDR L AMKRGYT LEYKSVIR++R +RP Sbjct: 241 SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVK 358 D+++ +D IVGFPGETEEDF+ +M LV ++ +D SFSFI+S RPGTPAANL DDTP+EVK Sbjct: 301 DLSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N ARIS + VG V+R+LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGQIIEVSITEAFPFSLRGEIV 443 RLIGQ+I+V I A+P SLRGE++ Sbjct: 421 HPRLIGQMIDVKINHAYPHSLRGELL 446