BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0068.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 158 9e-39 PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 155 6e-38 ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escher... 154 1e-37 STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmon... 144 2e-34 YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersin... 136 4e-32 mlr5029 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 114 1e-25 ZyigK [E] COG1280 Putative threonine efflux protein EcZ Escheric... 103 3e-22 rhtB [E] COG1280 Putative threonine efflux protein Eco Escherich... 103 3e-22 AGc3421 [E] COG1280 Putative threonine efflux protein Atu Agroba... 102 5e-22 SMc01851 [E] COG1280 Putative threonine efflux protein Sme Sinor... 98 2e-20 >VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 205 Score = 158 bits (399), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 84/213 (39%), Positives = 120/213 (56%), Gaps = 13/213 (6%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 MD W A+ A V +++PGSG V S+S+GL+YG R + I+GL+I L +++ G G Sbjct: 1 MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+L+ S AF +IK GA YL++LG +WR R P + + + A+ A Sbjct: 61 IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKA----- 115 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 L N TNPK IVF+VA+ PQFID R W Q LVL +T V +D +VM GY A Sbjct: 116 ------VLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTALA 169 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGAGLFF 213 ++L R+ RS + N+ FG M +G G+ Sbjct: 170 AQLGRYIRSPNIMTRMNKLFGS--MFMGCGMLL 200 >PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 209 Score = 155 bits (392), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 92/209 (44%), Positives = 120/209 (57%), Gaps = 12/209 (5%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M TWFAFF A I++SPG+GA+ SMS GL YG R LGL+I L + + I AG Sbjct: 1 MLVSTWFAFFLACWAISLSPGAGAIASMSCGLQYGFARGYWNALGLQIGLALQIAIVAAG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 VG+LL S AF++IK G YL+YL QW+A Q + D R Sbjct: 61 VGALLATSALAFSLIKWFGVAYLVYLAVRQWQA----PPQALSTDGERPLGRPLT----- 111 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 + GFL NA+NPK ++FM+AVLPQFID +PL Q L++ TM+ VDL+VM GY A Sbjct: 112 -LVLRGFLVNASNPKAVIFMLAVLPQFIDPHQPLLAQYLIMGGTMIVVDLIVMAGYTGLA 170 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGA 209 +R+ R RS + + NR F + VGA Sbjct: 171 ARVLRVLRSPRQQKLVNRTFAS--LFVGA 197 >ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escherichia coli O157:H7 Length = 206 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 85/209 (40%), Positives = 127/209 (60%), Gaps = 11/209 (5%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M + WFA+ + ++++SPGSGA+ +M+ LN+G R A+I GL+ L + +++ G G Sbjct: 1 MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+L S AF V+K AGA YLI+LG QWRA A+D + A +R+ + Sbjct: 61 LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRA-------AGAID-LKSLASTQSRRHLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 QR + N TNPK IVF+ A+ PQFI +P +Q +VL VT + VD++VM GYA A Sbjct: 113 QRAV---FVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGA 209 R+ + + K ++A N+ FG + MLVGA Sbjct: 170 QRIALWIKGPKQMKALNKIFGSLFMLVGA 198 >STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmonella typhimurium LT2 Length = 206 Score = 144 bits (362), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 83/209 (39%), Positives = 127/209 (60%), Gaps = 11/209 (5%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M ++ WFA+ + ++++SPGSGA+ +M+ +N+G R A+I GL+ L + +++ G G Sbjct: 1 MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAVASIAGLQTGLGIHIVLVGVG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+L S AF ++K AGA YLI+LG QWRA A+D + A+ +R + Sbjct: 61 LGTLFSRSLLAFEILKWAGAAYLIWLGIQQWRA-------AGAID-LHTLAQTQSRGRLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 +R + N TNPK IVF+ A+ PQFI +P Q L+L VT + VD+VVM GYA A Sbjct: 113 KRAI---FVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMVVMTGYATLA 169 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGA 209 R+ + + K ++A N+ FG + MLVGA Sbjct: 170 QRIAAWIKGPKQMKALNKAFGSLFMLVGA 198 >YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 206 Score = 136 bits (342), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 81/209 (38%), Positives = 126/209 (60%), Gaps = 11/209 (5%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M W + ++++SPGSGA+ +MS +++G R A+I GL++ L V +++ G G Sbjct: 1 MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVVASIGGLQLGLAVHIVLVGVG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+L+ S AF ++K GA YLI+LG QWRA +LD +A A + R+ + Sbjct: 61 LGALVSQSLLAFEILKWLGAAYLIWLGIQQWRA-------AGSLD-LHALANSMPRRKLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 +R + N TNPK IVF+ A+ PQF+ +P Q L+L T V VD++VM GYA A Sbjct: 113 KRAV---FVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGA 209 +R+ R+ +S + ++ NR FGG+ ML+GA Sbjct: 170 TRIARWIKSPQQMKLLNRIFGGLFMLIGA 198 >mlr5029 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 207 Score = 114 bits (285), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 76/211 (36%), Positives = 110/211 (52%), Gaps = 13/211 (6%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M ++ W AF AA+ ++ + P +L +S+ L G R +G+ + + ++ G Sbjct: 1 MSFENWAAFAAASTILLVIPDPTILLVVSYALGQGWRTALPMAVGVALGDFTAMTLSMLG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+LL AS T F V+K+ GA YLIYLG +RA + + ++A AHA Sbjct: 61 IGALLAASATVFTVLKLIGAGYLIYLGVKLFRAGGALKAEPRTDVVSSAKMMAHA----- 115 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 +L A NPK I F VA LPQF+D W Q+L+ T +A+ GYA A Sbjct: 116 ------WLVTALNPKSITFFVAFLPQFLDRHADFWTQMLIFETTFLALAFANAFGYALIA 169 Query: 181 SRLQRFFRSQKAIRAQNRFFGGVLMLVGAGL 211 +R + R+ KAIR NR GG L LVGAG+ Sbjct: 170 ARARNVVRNPKAIRIFNR-TGGTL-LVGAGI 198 >ZyigK [E] COG1280 Putative threonine efflux protein EcZ Escherichia coli O157:H7 EDL933 Length = 138 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 61/141 (43%), Positives = 84/141 (59%), Gaps = 11/141 (7%) Query: 71 AFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTGFLTN 130 AF V+K AGA YLI+LG QWRA A+D + A +R+ +QR + N Sbjct: 3 AFEVLKWAGAAYLIWLGIQQWRA-------AGAIDL-KSLASTQSRRHLFQRAV---FVN 51 Query: 131 ATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAASRLQRFFRSQ 190 TNPK IVF+ A+ PQFI +P +Q +VL VT + VD++VM GYA A R+ + + Sbjct: 52 LTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLAQRIALWIKGP 111 Query: 191 KAIRAQNRFFGGVLMLVGAGL 211 K ++A N+ FG + MLVGA L Sbjct: 112 KQMKALNKIFGSLFMLVGALL 132 >rhtB [E] COG1280 Putative threonine efflux protein Eco Escherichia coli K12 Length = 138 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 61/141 (43%), Positives = 84/141 (59%), Gaps = 11/141 (7%) Query: 71 AFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTGFLTN 130 AF V+K AGA YLI+LG QWRA A+D + A +R+ +QR + N Sbjct: 3 AFEVLKWAGAAYLIWLGIQQWRA-------AGAIDL-KSLASTQSRRHLFQRAV---FVN 51 Query: 131 ATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAASRLQRFFRSQ 190 TNPK IVF+ A+ PQFI +P +Q +VL VT + VD++VM GYA A R+ + + Sbjct: 52 LTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLAQRIALWIKGP 111 Query: 191 KAIRAQNRFFGGVLMLVGAGL 211 K ++A N+ FG + MLVGA L Sbjct: 112 KQMKALNKIFGSLFMLVGALL 132 >AGc3421 [E] COG1280 Putative threonine efflux protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 273 Score = 102 bits (255), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 64/198 (32%), Positives = 96/198 (48%), Gaps = 8/198 (4%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M + W AF AA+ ++ PG +L +S+ L +G + ++AT+ G+ + + + G Sbjct: 62 MPLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLG 121 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+LL S F +K GA YLIYLG WR+ V GE D + R + + Sbjct: 122 LGALLATSAALFTGLKWIGAAYLIYLGIKLWRSPV----GGEGADGQGVTGRERPLKIF- 176 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 + ++ A NPK IVF VA LPQF+ P W Q+L+ T + + V Y A Sbjct: 177 ---LHAYIVTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVLATVNAALYGLLA 233 Query: 181 SRLQRFFRSQKAIRAQNR 198 S + R K R NR Sbjct: 234 SAARNTIRKPKVQRIVNR 251 >SMc01851 [E] COG1280 Putative threonine efflux protein Sme Sinorhizobium meliloti Length = 211 Score = 97.8 bits (242), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 70/214 (32%), Positives = 107/214 (50%), Gaps = 18/214 (8%) Query: 1 MDYQTWFAFFAAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAG 60 M ++ WFAF AA+ V+ PG +L +S+ L +G + AT+ G+ + + + G Sbjct: 1 MSFEHWFAFAAASAVLLAIPGPTILLVISYALGHGRKIAGATVAGVALGDFTAMTASMLG 60 Query: 61 VGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWW 120 +G+LL S F V+K GA YL++LG WRA V + G ++ + A Sbjct: 61 LGALLATSAAVFTVLKWIGAAYLVWLGIKLWRAPV-GNDSGSTVETSPA----------- 108 Query: 121 QRCMTGFL----TNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGY 176 +R + FL A NPK I+F VA LPQF+D RPL+ Q+ + T + + + Y Sbjct: 109 ERPLRIFLHTYAVTALNPKSILFFVAFLPQFLDLSRPLFAQMAIFETTFLILATINAALY 168 Query: 177 AFAASRLQRFFRSQKAIRAQNRFFGGVLMLVGAG 210 A+ A+ R R NR G +L+GAG Sbjct: 169 AWLAAAAGSTIRKPNIRRIVNRLGGS--LLIGAG 200