BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0099.p (502 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0367_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) ac... 181 5e-45 Z4856 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid l... 149 2e-35 ECs4331 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 149 2e-35 RSc0432_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) ac... 107 7e-23 XF1038 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 102 2e-21 Z4855 [S] COG4648 Predicted membrane protein EcZ Escherichia col... 88 4e-17 ECs4330 [S] COG4648 Predicted membrane protein Ecs Escherichia c... 88 4e-17 NMA1954 [S] COG4648 Predicted membrane protein NmA Neisseria men... 85 4e-16 RSp0368 Non COG 75 5e-13 >RSp0367_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Rso Ralstonia solanacearum Length = 438 Score = 181 bits (458), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 137/375 (36%), Positives = 180/375 (48%), Gaps = 19/375 (5%) Query: 53 QRMALFHHDTLQFAAALFAIWLAGKTAYLPSDALPDTCRQLQGEVDAFLGEFGAHCPALH 112 R AL D FA ALFA+ +GK +P+ + P + D+ L + Sbjct: 48 NRYALCIDDPFAFACALFALLASGKAVVIPASSAPAYLASIADAYDSALTDGDME----- 102 Query: 113 PAGDAAEGDTPDAWPALR--ADFAGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGA 170 G ++ P LR A A + +YTSGS+ +AI K L Q EV TLE +G Sbjct: 103 --GIISQAAQPPGAALLRPIAPHAPITLYTSGSSAAPKAIHKTLAQFDAEVRTLEDHWGE 160 Query: 171 IVGEAEVIATVSHQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRMAGARPW-ILLSSP 229 +G ++A+V H HIYGLLF++ W L +GR + P LQ R ++SSP Sbjct: 161 WIGPGAILASVPHHHIYGLLFRIFWPLASGRAFGRRTYAEPHALQTALTRRETAAIVSSP 220 Query: 230 AHLKRLPEQSTQPDARQL-RAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWR 288 A L R P L A+FSSGGPL AAA A G P EIYGS+ETGGIAWR Sbjct: 221 AQLARWPSLPGFDTLSPLPAAVFSSGGPLDEAAAATFASAHGTAPTEIYGSTETGGIAWR 280 Query: 289 QPQTDPLQRQEHWQAMPGVEVRLGAEGELEVRSPHLPDSAWFGMADRAALVPGQPGQFEL 348 + + WQA V VR A+G L VRSPHL W D A G+F L Sbjct: 281 R-----RNESDAWQAFANVAVRREADGALSVRSPHLGHPDWHRTDDAAEF--DDAGRFRL 333 Query: 349 RGRSDRIVKLEEKRISLTRIEQLLNASPLVEDVRVIVHQGRRARQFIAAFVVPSAPAHTM 408 RGR DRIVKL+ KR+SL IE L P V ++ G +R+ + ++ Sbjct: 334 RGRMDRIVKLDGKRVSLPEIENWLGLHPYVAQSAAVLLPG-ASRERLGVLSALTSAGVEA 392 Query: 409 LAAQGKLALTTVIER 423 L G++ L + R Sbjct: 393 LRQHGRIGLAKTLRR 407 >Z4856 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases EcZ Escherichia coli O157:H7 EDL933 Length = 453 Score = 149 bits (375), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 131/391 (33%), Positives = 185/391 (47%), Gaps = 24/391 (6%) Query: 23 IGWRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHHDTLQFAAALFAIWLAGKTAYLP 82 I W + T R ++ + +Q PG+R AL ++ F AL A AGKT LP Sbjct: 21 IAWLNESTWTLGDLRHDVAQLICRLQQQPGERWALCFENSYLFIVALLATLHAGKTPVLP 80 Query: 83 SDALPDTCRQLQGEVDAFLG-----EFGAHCPALHPAGDAAEGDTPDAWPALRADFAGLV 137 + QL + + F G E L A + A+ A A + Sbjct: 81 GH---NRVIQLNEQRELFDGVLSDSELNWQGSLLLVASSPQIATQSFTFAAI-APEAFIE 136 Query: 138 VYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEAEVIATVSHQHIYGLLFKVLWAL 197 ++TSGSTG + K + + E L GA + V+ +V QH+YGL F+V + Sbjct: 137 LFTSGSTGQPKRAIKPVHLLDREAELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPM 196 Query: 198 CAGRPVHAGSAFFPEDLQRMAGARPWILLSSPAHLKRLPEQSTQPDARQLRAIFSSGGPL 257 G P+HA ++ E ++ +I +SSPA LKRL Q + P ++ + S+GG L Sbjct: 197 ALGLPLHAAMLWYVEQFAALSHQHRYIFISSPAFLKRLDTQLSPPP---VQVLLSAGGEL 253 Query: 258 PAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEHWQAMPGVEVRLGAEGE- 316 P Q +A L P EIYGS+ETG IAWR + Q Q W +PG++ + AEG+ Sbjct: 254 PWQDVQHTASWLRVWPDEIYGSTETGVIAWRYRE----QEQRRWLPLPGMQFQ--AEGDA 307 Query: 317 LEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEEKRISLTRIEQ-LLNAS 375 + SP + + GM L + GQF L GR RIVK+EEKRISL +EQ LL Sbjct: 308 FRLFSPLIAEDN--GMLLDDILQFSEDGQFHLMGRRGRIVKIEEKRISLQEVEQRLLALD 365 Query: 376 PLVEDVRVIVHQGRRARQFIAAFVVPSAPAH 406 + E V V +G RQ I +V AH Sbjct: 366 GIREAAAVPVTRG--GRQSIGVLLVLDDEAH 394 >ECs4331 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Ecs Escherichia coli O157:H7 Length = 453 Score = 149 bits (375), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 131/391 (33%), Positives = 185/391 (47%), Gaps = 24/391 (6%) Query: 23 IGWRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHHDTLQFAAALFAIWLAGKTAYLP 82 I W + T R ++ + +Q PG+R AL ++ F AL A AGKT LP Sbjct: 21 IAWLNESTWTLGDLRHDVAQLICRLQQQPGERWALCFENSYLFIVALLATLHAGKTPVLP 80 Query: 83 SDALPDTCRQLQGEVDAFLG-----EFGAHCPALHPAGDAAEGDTPDAWPALRADFAGLV 137 + QL + + F G E L A + A+ A A + Sbjct: 81 GH---NRVIQLNEQRELFDGVLSDSELNWQGSLLLVASSPQIATQSFTFAAI-APEAFIE 136 Query: 138 VYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEAEVIATVSHQHIYGLLFKVLWAL 197 ++TSGSTG + K + + E L GA + V+ +V QH+YGL F+V + Sbjct: 137 LFTSGSTGQPKRAIKPVHLLDREAELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPM 196 Query: 198 CAGRPVHAGSAFFPEDLQRMAGARPWILLSSPAHLKRLPEQSTQPDARQLRAIFSSGGPL 257 G P+HA ++ E ++ +I +SSPA LKRL Q + P ++ + S+GG L Sbjct: 197 ALGLPLHAAMLWYVEQFAALSHQHRYIFISSPAFLKRLDTQLSPPP---VQVLLSAGGEL 253 Query: 258 PAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEHWQAMPGVEVRLGAEGE- 316 P Q +A L P EIYGS+ETG IAWR + Q Q W +PG++ + AEG+ Sbjct: 254 PWQDVQHTASWLRVWPDEIYGSTETGVIAWRYRE----QEQRRWLPLPGMQFQ--AEGDA 307 Query: 317 LEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEEKRISLTRIEQ-LLNAS 375 + SP + + GM L + GQF L GR RIVK+EEKRISL +EQ LL Sbjct: 308 FRLFSPLIAEDN--GMLLDDILQFSEDGQFHLMGRRGRIVKIEEKRISLQEVEQRLLALD 365 Query: 376 PLVEDVRVIVHQGRRARQFIAAFVVPSAPAH 406 + E V V +G RQ I +V AH Sbjct: 366 GIREAAAVPVTRG--GRQSIGVLLVLDDEAH 394 >RSc0432_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Rso Ralstonia solanacearum Length = 456 Score = 107 bits (267), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 122/392 (31%), Positives = 164/392 (41%), Gaps = 44/392 (11%) Query: 13 PFLAFAGAD-VIGWRDGVAVTHASWRAELRRWQHLVQTTP-GQRMALFHHDTLQFAAALF 70 P + A D I WRDG V ++ AE+ L P G + D +F L Sbjct: 22 PLVFHASPDQTIAWRDGAPVAVRTFLAEV---AQLAAALPAGAHVFNACTDRYRFTVGLC 78 Query: 71 AIWLAGKTAYLPSDALPDTCRQLQG-EVDAFLGEFGAHC----------PALHPAGDAAE 119 A +AGK + LP+ P+T R+L D F C L PAG Sbjct: 79 ATLVAGKISLLPASQTPETVRRLAAFAPDVFCLHDAPDCAIALPRFRFPEPLPPAG---- 134 Query: 120 GDTPDAWPALRADFAGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEA-EVI 178 D P P + A ++TSGSTG K G + V A G + A ++ Sbjct: 135 ADAPVDVPQIDASRVMAYIFTSGSTGTPVPHRKTWGALVEGVRASAARLGLLDAHACTLV 194 Query: 179 ATVSHQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRMAGA--RPWILLSSPAHLKRL- 235 TV QH+YG VL AL G F+P D++ A +P +L++SP HL+ L Sbjct: 195 GTVPPQHMYGFESTVLLALVGGLAFSNRLPFYPADIRAALEAVPQPRVLVTSPIHLRALL 254 Query: 236 -PEQSTQPDARQLRAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDP 294 E++ P A L A + PL ++A A A L +EIYGS+ETG IA R+ Sbjct: 255 SSERALPPAALVLSATAALPEPLASEAEARLAAPL----IEIYGSTETGQIATRR----- 305 Query: 295 LQRQEHWQAMPGV--EVRLGAEGELE----VRSPHLPDSAWFGMADRAALVPGQPGQFEL 348 + W PG+ E R G E HL A M D L+ F L Sbjct: 306 TAQGATWHLQPGIRLEARAGTPDEDGPVTWASGAHL--DAPVPMGDTLELI--DDTHFLL 361 Query: 349 RGRSDRIVKLEEKRISLTRIEQLLNASPLVED 380 GR ++ + KR SL + LNA P VED Sbjct: 362 HGRKADLINVAGKRTSLAYLNHQLNAIPGVED 393 >XF1038 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Xfa Xylella fastidiosa 9a5c Length = 503 Score = 102 bits (254), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 106/372 (28%), Positives = 169/372 (45%), Gaps = 39/372 (10%) Query: 25 WRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHHDTLQFAAALFAIWLAGKTAYLPSD 84 W G +T + A++R Q G+ + D +F A +A L G+ + LPS Sbjct: 80 WVHGKPITLGRFLAQVRGLA--AQLPSGRYVVNLCEDRYRFLLAFYACALRGQVSLLPSS 137 Query: 85 ALPDTCRQLQGEV-DAF-LGEFGA--------HCPALHPAGDAAEGDTPDAWPALRADFA 134 P ++ D++ LG+ H P + P D A P+L D Sbjct: 138 RAPGVVAEIYAAYPDSYCLGDRELSLESLRYWHLPEIFPELDGA-------IPSLSEDAV 190 Query: 135 GLVVYTSGSTGLAQAIPKQLGQMANEV----ATLEALFGAIVGEAEVIATVSHQHIYGLL 190 + +TSGSTG+ + K G + +L+G+ V V+ATV QH+YG+ Sbjct: 191 VAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWVAFHSLWGSQV--PTVVATVPPQHMYGME 248 Query: 191 FKVLWALCAGRPVHAGSAFFPEDL-QRMAGA-RPWILLSSPAHLKRLPEQSTQPDARQLR 248 F VL L A V+AG FFPED+ Q +A P +L+++P HL+ L + + ++ Sbjct: 249 FSVLLPLLAPVAVYAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLR--FPEVA 306 Query: 249 AIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEHWQAMPGVE 308 I S+ PL A + G E++GS+ET A R+ ++ W GV Sbjct: 307 GIVSATAPLTHDLAVAAEACFGCELREVFGSTETCTFAVRR-----TVYEQAWTRFDGVS 361 Query: 309 VRLGAEGELEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEEKRISLTRI 368 + + A+ V + HLP A +AD + + G+FE+ GR ++++ KR SL + Sbjct: 362 IEVNADVTW-VYARHLP--APMAIADFVDVY--EDGRFEVLGRQADLLEIAGKRASLGEL 416 Query: 369 EQLLNASPLVED 380 + L A P V D Sbjct: 417 NRCLLAIPGVVD 428 >Z4855 [S] COG4648 Predicted membrane protein EcZ Escherichia coli O157:H7 EDL933 Length = 193 Score = 88.2 bits (217), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 42/83 (50%), Positives = 59/83 (71%) Query: 420 VIERMARLGSPDLPLFVIAYTRRVTQVWCLFFAINGSIALGTALWCSPAVWSMYTGVISY 479 ++ER+ARL PDLP + YTR VTQ+WC FF ING IAL TAL+ ++W+ + G+I+Y Sbjct: 111 IVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSLWTAWNGMIAY 170 Query: 480 IAMGLLFAGEYLVRLYVRRQHHA 502 + MG L AGE+L+R V ++ A Sbjct: 171 LLMGTLMAGEWLLRRQVMKRDRA 193 >ECs4330 [S] COG4648 Predicted membrane protein Ecs Escherichia coli O157:H7 Length = 193 Score = 88.2 bits (217), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 42/83 (50%), Positives = 59/83 (71%) Query: 420 VIERMARLGSPDLPLFVIAYTRRVTQVWCLFFAINGSIALGTALWCSPAVWSMYTGVISY 479 ++ER+ARL PDLP + YTR VTQ+WC FF ING IAL TAL+ ++W+ + G+I+Y Sbjct: 111 IVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSLWTAWNGMIAY 170 Query: 480 IAMGLLFAGEYLVRLYVRRQHHA 502 + MG L AGE+L+R V ++ A Sbjct: 171 LLMGTLMAGEWLLRRQVMKRDRA 193 >NMA1954 [S] COG4648 Predicted membrane protein NmA Neisseria meningitidis Z2491 Length = 144 Score = 85.1 bits (209), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 45/80 (56%), Positives = 56/80 (70%), Gaps = 10/80 (12%) Query: 419 TVIERMARLGSPDLPLFVIAYTRRVTQVWCLFFAINGSIA-----LGTALWCSPAVWSMY 473 TVIER+ARL PDLP + YTRRVTQVWC+FF NG++A LG W W++Y Sbjct: 64 TVIERLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTLAALLARLGRYDW-----WAVY 118 Query: 474 TGVISYIAMGLLFAGEYLVR 493 TGVI+Y+ MG+ FAGE+L R Sbjct: 119 TGVIAYVLMGMPFAGEWLYR 138 >RSp0368 Non COG Length = 205 Score = 74.7 bits (182), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 38/82 (46%), Positives = 51/82 (62%) Query: 419 TVIERMARLGSPDLPLFVIAYTRRVTQVWCLFFAINGSIALGTALWCSPAVWSMYTGVIS 478 +++E+ ARL +P+LP + YTR VTQVWC FFA+N IA AL+ S W++Y G I+ Sbjct: 117 SMVEKFARLRTPELPPRAVRYTRHVTQVWCGFFAVNALIAALVALYGSRQAWALYNGAIA 176 Query: 479 YIAMGLLFAGEYLVRLYVRRQH 500 Y + LL GE R V R H Sbjct: 177 YALVCLLIVGEIAYRNLVVRPH 198