BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0016.p (539 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc3078 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 370 e-102 SMb20650 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-aci... 326 1e-88 mll6742 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 318 2e-86 BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 256 8e-68 BH3104 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 206 8e-53 Rv2505c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 193 7e-49 MT2580 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 193 7e-49 BH3103 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 192 2e-48 RSc0442 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 191 3e-48 AF2368 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 190 9e-48 >AGc3078 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 510 Score = 370 bits (950), Expect = e-102, Method: Compositional matrix adjust. Identities = 231/539 (42%), Positives = 306/539 (56%), Gaps = 41/539 (7%) Query: 1 VTPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY 60 +TP + LLA A+A + AL ++ Q L Y A AAAL G G+RV +Y Sbjct: 1 MTPHFLLHHLLA-ARAASEDQALVHKEQSLTYREFAEAAAHCAAALHEAGTERGDRVVIY 59 Query: 61 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLS 120 L + E ++FG + A VFVP+N LLK Q+ HI+ DC +++++S L L+ Sbjct: 60 LPRGFEECWSIFGVSMASGVFVPVNALLKAQQIRHIVTDCGAKIVISSMAMMDELKAALT 119 Query: 121 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 P + T++L + A + PA P+ AA G D+AA Sbjct: 120 DLPDI-TILLAEEITARKSAPARPS---------------AAIG-----------EDLAA 152 Query: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 ILYTSGSTG PKGV+LSHRNL+AGA+ V YL +DR+L++LP SFDYGL+QL TA Sbjct: 153 ILYTSGSTGSPKGVMLSHRNLLAGARIVRTYLDITGKDRILSLLPFSFDYGLNQLLTAVE 212 Query: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQ-------QLAGLPWPQGCSLRYLT 293 GA+ + + +I+ + + ITGLAGVP +W LA P P LRYLT Sbjct: 213 QGATTIISTFRLGDEIVRDLRDQAITGLAGVPTIWAILTRAAPLLAKTPLPH---LRYLT 269 Query: 294 NSGGALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIM 353 NSGG +P+ + LR LP +++LMYGLTEAFRST+LPP E+DRRP SIG+AIP EI Sbjct: 270 NSGGRVPQETVKALREKLPDTKIYLMYGLTEAFRSTFLPPDEIDRRPTSIGKAIPECEIF 329 Query: 354 VVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGD 413 +V G PGE G LVHRG V+ GYWN P TA+ RP P AA G E +SGD Sbjct: 330 IVTDKGQRAKPGEPGILVHRGPTVSLGYWNRPEDTAKVLRPHPFIPAARGG-ETVCYSGD 388 Query: 414 SVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVV 473 DE G+ FV R D MIK SGYRISP EVEE L +TGL + AV+G+ P G+ V Sbjct: 389 LAVEDEDGFFSFVARNDAMIKSSGYRISPTEVEESLMSTGLFREVAVIGLPDPFAGEKVH 448 Query: 474 AVAP-SSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADH 531 AVA +S T + A + L +M+P A+ LP + NGK+D + + + D+ Sbjct: 449 AVATAASETIDVSAALKKAAEMLAPFMIPRAIE-LVERLPITANGKVDYRALVRERTDN 506 >SMb20650 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Sme Sinorhizobium meliloti Length = 515 Score = 326 bits (835), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 208/527 (39%), Positives = 290/527 (55%), Gaps = 30/527 (5%) Query: 10 LLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVL 69 L+ +A A TAL ++L YA L ++AAA G+ +RV V+++ E Sbjct: 7 LIRNAAANGAKTALVTDRRRLGYAELDDLSTRLAAAFAENGVKRNDRVLVFMDNCWEAAA 66 Query: 70 ALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVV 129 A+F AG F PIN K ++++I+ DC ++T P +A+ L+ P Sbjct: 67 AIFAILKAGATFSPINASTKADKLAYIVADCEAAAILTQAKLMPVVAEALALAPG----- 121 Query: 130 LVDAAAAAPATPAPPAPLGRL--ATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGS 187 AP + AP GR+ + L AAP P H ID D+ ++YTSGS Sbjct: 122 ------HAPFVASTAAPGGRIPDGAASFEECLTAAPAPVR---HGGIDVDLGMLIYTSGS 172 Query: 188 TGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTL 247 TG+PKGV+++HRN+ A ++S+ YL N P D +L VLPL+FDYGL QL A GA++ L Sbjct: 173 TGRPKGVMMTHRNIDAASESITTYLRNTPDDIILNVLPLAFDYGLYQLLMAIRLGATLVL 232 Query: 248 LNYLA-PRDILHAVASEGITGLAGVPPL---WQQLAGLPWPQGCSLRYLTNSGGALPRSV 303 A P+ I + +E +TG VP + Q+ L SLRYL+N+ ALP + Sbjct: 233 EKSFAFPQAIFDRIRTERVTGFPLVPTMAAMILQMRDLEPGFLPSLRYLSNTAAALPPAH 292 Query: 304 LAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCA 363 +A+LR P A+++ MYGLTE R TYLPP ELDRRP S+G AIPN E VV +G+ Sbjct: 293 IARLRELFPGARLYSMYGLTECKRCTYLPPEELDRRPGSVGIAIPNTEAFVVDDEGNRVP 352 Query: 364 PGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTP-ELAVWSGDSVRMDEQGY 422 PG GELV RG V QGYW + A T RP P P E + +GD R DE+G+ Sbjct: 353 PGVPGELVIRGPHVMQGYWRNDAATERMLRPGP-------NPWEKVLHTGDLFRTDEEGF 405 Query: 423 LYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVA-VAPSSCT 481 LYFVGR DD+IK G +++P EVE VLHA + V+GV P G A+ A V S T Sbjct: 406 LYFVGRKDDIIKTRGEKVAPKEVETVLHAHPGIAEAVVIGVPDPVLGAAIGALVVLSDPT 465 Query: 482 ASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTL 528 + + + + C HL +M+P + + ELP++ GK+ R+L +TL Sbjct: 466 LTEKDIIRHCSRHLEDFMVPKIVE-FRTELPKTDTGKVSRRLAAETL 511 >mll6742 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mlo Mesorhizobium loti Length = 511 Score = 318 bits (815), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 195/525 (37%), Positives = 282/525 (53%), Gaps = 26/525 (4%) Query: 5 LIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKR 64 ++HD L SA+ AL Q++ Y L A +A L A G+ G+RV ++ + Sbjct: 1 MLHDYLAHSARRLGDKVALVCGKQRVTYGELEARANAIATDLAASGVARGDRVMIFADNT 60 Query: 65 METVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPA 124 +ETV++ + A V +NPL K +V ++L DC L+T H + C + Sbjct: 61 VETVVSFWAVLKANAVVCIVNPLTKSDKVDYLLNDCRPAALITDAHLHAIFREPARGCQS 120 Query: 125 LQTVVLVDAAAAAPATPAPPAPLGRLATLDWN-AFLQAAPGPGSRRAHRRIDADIAAILY 183 L +++ + + P A + W+ A P +RR ID D+AAI+Y Sbjct: 121 LLRMIVSGSIDDPELSRLPRA-------VRWDTAVGNGGDAPPARRC---IDIDLAAIIY 170 Query: 184 TSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGA 243 TSGSTG+PKGV+L+HRN++ S+A YL + +L VLPL+FDYGL Q+ AF +GA Sbjct: 171 TSGSTGEPKGVMLTHRNMMTACTSIASYLELAEDEVILNVLPLAFDYGLYQMIMAFRTGA 230 Query: 244 SVTLLNYLA-PRDILHAVASEGITGLAGVPPLW---QQLAGLPWPQGCSLRYLTNSGGAL 299 + L A P +L + EGITG GVP ++ +L L S+RY+TN+ AL Sbjct: 231 RLVLERSFAFPAQVLGLIRQEGITGFPGVPTIFAALSELRSLKDQDFSSIRYVTNTAAAL 290 Query: 300 PRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDG 359 P + LR A+++ MYGLTE R TYLPP +L R+P S+G AIPN E+ +V Sbjct: 291 PLKHILMLRELFSSARIYSMYGLTECKRCTYLPPEDLKRKPLSVGIAIPNTEMWIVDEHD 350 Query: 360 SPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTP-ELAVWSGDSVRMD 418 PG VG+LV RGA V +GYW P TA + +P P P E +++GD RMD Sbjct: 351 RRVGPGVVGQLVIRGATVMKGYWGKPEATARKLKPGP-------LPGEQVLYTGDYCRMD 403 Query: 419 EQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVA--VA 476 +GYLYFVGR D++IK G +++P EVE VL + AV+GV GQAV A V Sbjct: 404 AEGYLYFVGRGDEIIKSRGEKVAPKEVENVLMDIPGVREAAVIGVPDELLGQAVKAFVVM 463 Query: 477 PSSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDR 521 + T + +Q C+ L ++M+P ++ LP + GKL + Sbjct: 464 ENGRTIGEKQLQMECQKRLENFMVPKSIV-VVASLPMTDTGKLKK 507 >BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 513 Score = 256 bits (655), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 161/524 (30%), Positives = 254/524 (48%), Gaps = 24/524 (4%) Query: 10 LLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVL 69 L +A +P + A R++ + Y L +++ A L G+ G+ +A+ L + ++ Sbjct: 7 LEETASEKPDSIACRFKDHMMTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFII 66 Query: 70 ALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVV 129 A FGA AG+V VPINPL P ++ ++L + V+ +V P P ++ V+ Sbjct: 67 AFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPKVELVI 126 Query: 130 LVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTG 189 L A P P R+ + L+ P +++ + D A ILYTSG+TG Sbjct: 127 LCQTGEAEPEAADPEV---RMKMTTFAKILR--PTSAAKQNQEPVPDDTAVILYTSGTTG 181 Query: 190 QPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTA-FLSGASVTLL 248 +PKG +L+H+NL + A VAGYLG +D ++ LP+ + L+ A +SGA+V + Sbjct: 182 KPKGAMLTHQNLYSNANDVAGYLGMDERDNVVCALPMCHVFCLTVCMNAPLMSGATVLIE 241 Query: 249 NYLAPRDILHAVASEGITGLAGVPPLWQQL---AGLPWPQGCSLRYLTNSGGALPRSVLA 305 +P + V + T AGVP ++ L S+R + G ++P ++L Sbjct: 242 PQFSPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKDDFSSIRLCISGGASMPVALLT 301 Query: 306 QLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPG 365 + YGL+EA T P + R+P SIG +I + E VV P G Sbjct: 302 AFEEKF-GVTILEGYGLSEASPVTCFNPFDRGRKPGSIGTSILHVENKVVDPLGRELPAH 360 Query: 366 EVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYF 425 +VGEL+ +G V +GY+ P T + +++GD R DE GY Y Sbjct: 361 QVGELIVKGPNVMKGYYKMPMETEHALKDG------------WLYTGDLARRDEDGYFYI 408 Query: 426 VGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVA-VAPSSCTASL 484 V R DMI V GY + P EVEEVL++ + V+GV PQ G+AV V P + Sbjct: 409 VDRKKDMIIVGGYNVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKRSGVTE 468 Query: 485 EAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTL 528 E + Q C HL Y PAA+ + ++P++ GK+ R+ ++ L Sbjct: 469 EDIMQHCEKHLAKYKRPAAI-TFLDDIPKNATGKMLRRALRDIL 511 >BH3104 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bha Bacillus halodurans Length = 566 Score = 206 bits (525), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 157/545 (28%), Positives = 251/545 (46%), Gaps = 46/545 (8%) Query: 6 IHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRM 65 + L +A+ P A+++ G+++ Y L +Q L LG+ G+RVA+ L Sbjct: 26 LQSYLKQAAEEFPSHIAVQFLGKEMTYRELYEQAKQFGNQLKTLGISKGDRVAIMLANSP 85 Query: 66 ETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPAL 125 +++++ +GA G V V NPL ++ H L D +V+V + P ++ ++ + L Sbjct: 86 QSIISYYGALMIGAVVVQTNPLYVERELEHQLVDSGAKVIVCLDLVFPRVS-KVRKATQL 144 Query: 126 QTVVLVDAAAAAPATPAPPAPLGRLA-------------TLDWNAFLQAAPGPGSRRAHR 172 + V++ P P + TL + FLQ Sbjct: 145 EYVIVTSIKDYLPFPKNVLYPFVQKKQTGMKIDITYNENTLSFKKFLQGGVTTEPSEDVS 204 Query: 173 RIDADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAG----YLGNHPQDRLLAVLPLSF 228 D D+A + YT G+TG KGV+L+H NLVA ++ G Y ++ +L VLP Sbjct: 205 PTD-DLALLQYTGGTTGPAKGVMLTHANLVAN--TIQGLHWMYKSKRAEEVILCVLPFFH 261 Query: 229 DYGLSQ-LSTAFLSGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC 287 YG+S ++ A ++ + +L P+D+L A+ +T G P ++ L P Q Sbjct: 262 VYGMSAGMNLAIMNAYKMVILPKFNPKDVLKAIEKHKVTLFPGAPTMYVGLINDPSIQDY 321 Query: 288 SLRYLTN--SGGA-LPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIG 344 +L + SG A LP V + + L ++ YGLTE P R+ SIG Sbjct: 322 NLTSVEACISGAAPLPLEVQQKFEQ-LTGGKLVEGYGLTETSPVAIATPIWGKRKQRSIG 380 Query: 345 RAIPNAEIMVVRPD-GSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALG 403 P+ ++M++ + G P EVGEL RG V +GYWN P TA+ F+ Sbjct: 381 IPWPDTDVMIISAETGEPADVNEVGELAVRGPQVMKGYWNRPEETAQVFKDD-------- 432 Query: 404 TPELAVW--SGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVV 461 W +GD MDE GY Y V R DMI G+ I P E+EE+L+ + V+ Sbjct: 433 ------WFLTGDMGYMDEDGYFYIVDRKKDMIIAGGFNIYPREIEEILYEHDAIKEAVVI 486 Query: 462 GVAHPQQGQAVVA--VAPSSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKL 519 G +G+ V A V + S E + CR HL +Y +P L+ + ELP++ GK+ Sbjct: 487 GAPDAYRGETVKAFIVLKEGRSVSEEELDAFCRKHLAAYKVP-RLYEFREELPKTLVGKI 545 Query: 520 DRKLM 524 R+++ Sbjct: 546 LRRVL 550 >Rv2505c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 547 Score = 193 bits (491), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 168/562 (29%), Positives = 256/562 (45%), Gaps = 71/562 (12%) Query: 3 PTLIHDTLLASAQARPQATALRYRGQQ----------LDYAGLAAGVEQVAAALLALGLG 52 P+L+ T+ A+ A TA RY ++ +Y+ L V ++A L+ G+G Sbjct: 21 PSLLESTIGANLAA----TAARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIG 76 Query: 53 PGERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRH 112 PG+RV ++ R E VL + A G + V INP + +V + L+ V +++ Sbjct: 77 PGDRVGIWAPNRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASFK 136 Query: 113 PA-----LADRLSQCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPG-PG 166 A LA+ +CP L V+L+++ W+A A P P Sbjct: 137 DADYAAMLAEVGPRCPDLADVILLESDR-------------------WDALAGAEPDLPA 177 Query: 167 SRRAHRRID-ADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLP 225 ++ R+D +D I YTSG+T PKGV LSHRN++ V LG QDR+ +P Sbjct: 178 LQQTAARLDGSDPVNIQYTSGTTAYPKGVTLSHRNILNNGYLVGELLGYTAQDRICIPVP 237 Query: 226 LSFDYGLSQLSTAFLSGASVTLLNY--LAPRDILHAVASEGITGLAGVPPLWQQLAGLP- 282 +G+ + A S + ++ P L AV E T L GVP ++ GLP Sbjct: 238 FYHCFGMVMGNLAATSHGAAMVIPAPGFDPAATLRAVQDERCTSLYGVPTMFIAELGLPD 297 Query: 283 ---WPQGCSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAE--LD 337 + G SLR +G A P V+ ++ + V + YG+TE + A+ +D Sbjct: 298 FTDYELG-SLRTGIMAGAACPVEVMRKVISRMHMPGVSICYGMTETSPVSTQTRADDSVD 356 Query: 338 RRPDSIGRAIPNAEIMVVRPDGSPCAP-GEVGELVHRGALVAQGYWNDPARTAERFRPAP 396 RR ++GR P+ EI VV P P G VGE RG V GYWNDP +TAE A Sbjct: 357 RRVGTVGRVGPHLEIKVVDPATGETVPRGVVGEFCTRGYSVMAGYWNDPQKTAEVI-DAD 415 Query: 397 GREAALGTPELAVW--SGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGL 454 G W +GD MD GY+ GR D++ G ISP E+EE+LH Sbjct: 416 G------------WMHTGDLAEMDPSGYVRIAGRIKDLVVRGGENISPREIEELLHTHPD 463 Query: 455 ADQFAVVGVAHPQQGQAVVAVAPSSCTA---SLEAVQQACRAHLPSYMLPAALHRW-PGE 510 V+GV + G+ ++AV A ++E +++ C + + +P L W E Sbjct: 464 IVDGHVIGVPDAKYGEELMAVVKLRNDAPELTIERLREYCMGRIARFKIPRYL--WIVDE 521 Query: 511 LPRSPNGKLDRKLMQQTLADHF 532 P + GK+ + M+Q ++ Sbjct: 522 FPMTVTGKVRKVEMRQQALEYL 543 >MT2580 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II MtC Mycobacterium tuberculosis CDC1551 Length = 547 Score = 193 bits (491), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 168/562 (29%), Positives = 256/562 (45%), Gaps = 71/562 (12%) Query: 3 PTLIHDTLLASAQARPQATALRYRGQQ----------LDYAGLAAGVEQVAAALLALGLG 52 P+L+ T+ A+ A TA RY ++ +Y+ L V ++A L+ G+G Sbjct: 21 PSLLESTIGANLAA----TAARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIG 76 Query: 53 PGERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRH 112 PG+RV ++ R E VL + A G + V INP + +V + L+ V +++ Sbjct: 77 PGDRVGIWAPNRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASFK 136 Query: 113 PA-----LADRLSQCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPG-PG 166 A LA+ +CP L V+L+++ W+A A P P Sbjct: 137 DADYAAMLAEVGPRCPDLADVILLESDR-------------------WDALAGAEPDLPA 177 Query: 167 SRRAHRRID-ADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLP 225 ++ R+D +D I YTSG+T PKGV LSHRN++ V LG QDR+ +P Sbjct: 178 LQQTAARLDGSDPVNIQYTSGTTAYPKGVTLSHRNILNNGYLVGELLGYTAQDRICIPVP 237 Query: 226 LSFDYGLSQLSTAFLSGASVTLLNY--LAPRDILHAVASEGITGLAGVPPLWQQLAGLP- 282 +G+ + A S + ++ P L AV E T L GVP ++ GLP Sbjct: 238 FYHCFGMVMGNLAATSHGAAMVIPAPGFDPAATLRAVQDERCTSLYGVPTMFIAELGLPD 297 Query: 283 ---WPQGCSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAE--LD 337 + G SLR +G A P V+ ++ + V + YG+TE + A+ +D Sbjct: 298 FTDYELG-SLRTGIMAGAACPVEVMRKVISRMHMPGVSICYGMTETSPVSTQTRADDSVD 356 Query: 338 RRPDSIGRAIPNAEIMVVRPDGSPCAP-GEVGELVHRGALVAQGYWNDPARTAERFRPAP 396 RR ++GR P+ EI VV P P G VGE RG V GYWNDP +TAE A Sbjct: 357 RRVGTVGRVGPHLEIKVVDPATGETVPRGVVGEFCTRGYSVMAGYWNDPQKTAEVI-DAD 415 Query: 397 GREAALGTPELAVW--SGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGL 454 G W +GD MD GY+ GR D++ G ISP E+EE+LH Sbjct: 416 G------------WMHTGDLAEMDPSGYVRIAGRIKDLVVRGGENISPREIEELLHTHPD 463 Query: 455 ADQFAVVGVAHPQQGQAVVAVAPSSCTA---SLEAVQQACRAHLPSYMLPAALHRW-PGE 510 V+GV + G+ ++AV A ++E +++ C + + +P L W E Sbjct: 464 IVDGHVIGVPDAKYGEELMAVVKLRNDAPELTIERLREYCMGRIARFKIPRYL--WIVDE 521 Query: 511 LPRSPNGKLDRKLMQQTLADHF 532 P + GK+ + M+Q ++ Sbjct: 522 FPMTVTGKVRKVEMRQQALEYL 543 >BH3103 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bha Bacillus halodurans Length = 564 Score = 192 bits (488), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 161/552 (29%), Positives = 249/552 (45%), Gaps = 41/552 (7%) Query: 6 IHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRM 65 +HD + +A P AL + G+ + Y V++ AA L LGL GERV++ L Sbjct: 28 LHDLIKEAATTYPHHVALHFLGKTMTYQLFYEQVQRFAANLQQLGLQKGERVSIMLPNCP 87 Query: 66 ETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPAL 125 + V+A +G AG V V NPL +++H L D R +V + +P + P Sbjct: 88 QAVIAYYGVLVAGGVVVQTNPLYVERELNHQLIDSGARFIVCLDVLYPRVIKSREGAPLE 147 Query: 126 QTVVLVDAAAAAPATPAP----------PAPLGRLAT-LDWNAFLQAAPGPGSRRAHRRI 174 +V A P P P P G++ + F+ A + RI Sbjct: 148 HVIV----TAIKDYLPFPKNMIYPFIQKPKPKGKIGYGSHVHRFVPMAQSGSTNPTPVRI 203 Query: 175 D--ADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAG--YLGNHPQDRLLAVLPLSFDY 230 D D+A + YT G+TG KGV+L+H NLVA Y Q+R LAVLP Y Sbjct: 204 DPKEDLALLQYTGGTTGVAKGVMLTHYNLVANTIQCRKWMYKWRDGQERTLAVLPFFHVY 263 Query: 231 GL-SQLSTAFLSGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQG--- 286 G+ + ++ ++G+ + ++ P +L + + IT G P ++ L P + Sbjct: 264 GMTTSMNLTLMTGSELHIVPRFDPLQVLKMIDKKKITMFPGAPTMYIALINHPDLKKYDL 323 Query: 287 CSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRA 346 S+ + +LP V Q L ++ YGLTEA T +R+ SIG Sbjct: 324 SSIEACISGSSSLPAEV-QQTFEQLTGGRLVEGYGLTEASPVTNCNLVWGERKDQSIGVP 382 Query: 347 IPNAEIMVVRPDGSPCAP-GEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTP 405 P+ ++ + + P GE+GE+V RG V +GYWN P T + Sbjct: 383 WPDTDVHIRSLETGESLPSGEIGEVVVRGPQVMKGYWNRPEETNATLQNG---------- 432 Query: 406 ELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAH 465 +++GD DE+GY Y V R DMI G+ I P EVEEVL+A + V+GV Sbjct: 433 --WLYTGDMGYKDEEGYFYIVDRKKDMIIAGGFNIYPREVEEVLYAHAKVQEAVVIGVPD 490 Query: 466 PQQGQAVVA--VAPSSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGK-LDRK 522 +G+ V A V + S + + CR HL +Y +P + + +LP++ GK L R Sbjct: 491 EYRGETVKAFIVVKEGESVSEKELNDYCRTHLAAYKVPRK-YEFRDDLPKTMVGKVLKRV 549 Query: 523 LMQQTLADHFMK 534 L+++ +K Sbjct: 550 LIEEETKKQGIK 561 >RSc0442 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Rso Ralstonia solanacearum Length = 571 Score = 191 bits (486), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 165/562 (29%), Positives = 249/562 (44%), Gaps = 61/562 (10%) Query: 3 PTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLE 62 P L+ DT+ A QA R +G + + A V+ +AA L ALGL GER+ ++ Sbjct: 31 PALLADTVRRCPDA--QAVVFREQGVRCTWRAFAEQVDALAAGLHALGLQRGERIGIWSP 88 Query: 63 KRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQC 122 R E VL F A GL+ V INP + ++ + L + +V +E A + S+ Sbjct: 89 NRYEWVLTQFATARLGLILVNINPAYRLTELEYALNKVGCKAIVVAE------AFKTSRY 142 Query: 123 PALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRI-------- 174 + + + A A P +L L W + PG R I Sbjct: 143 LEMLQTLAPELATAQPGA----LRAAKLPALRWVIRMGEGATPGMIRFDEVIARGQGVPA 198 Query: 175 ---DADIAA--------ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAV 223 DA AA I +TSG+TG PKG L+H N+V A+ VA + DRL Sbjct: 199 GTLDAITAALSPDDAINIQFTSGTTGAPKGATLTHVNIVNNARFVAMAMNLQGTDRLCVP 258 Query: 224 LPLSFDYG--LSQLSTAFLSGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGL 281 +PL +G +S L+ A V P L AVA E T L GVP ++ +A L Sbjct: 259 VPLYHCFGMVMSVLACAATGACMVFPGEAFDPLATLRAVAEERCTALHGVPTMF--IAQL 316 Query: 282 PWPQG-----CSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTE----AFRSTYLP 332 P+ SLR +G P V+ ++ + ++ + YG+TE +F+S Sbjct: 317 DHPEFPSFDVSSLRGGIMAGSPCPIEVMKRVVAEMHLREITIAYGMTETSPVSFQSAVTD 376 Query: 333 PAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERF 392 P LD+R ++GR P+ ++ +V G GE GEL RG V GYW+D A+TAE Sbjct: 377 P--LDKRVTTVGRVQPHLQVKLVDGAGEVVPVGEKGELCTRGYSVMLGYWDDEAKTAESI 434 Query: 393 RPAPGREAALGTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHAT 452 + R +GD D GY VGR DM+ G + P E+EE L Sbjct: 435 QDGWMR------------TGDLATFDADGYCNIVGRVKDMLIRGGENVYPREIEEFLFRH 482 Query: 453 GLADQFAVVGVAHPQQGQAVVA--VAPSSCTASLEAVQQACRAHLPSYMLPAALHRWPGE 510 V GV P+ G+ V A V A+ E +++ C+ + Y +P + R+ E Sbjct: 483 PKVQAVNVFGVPDPKYGEEVCAWIVLKPGQQATQEEIREFCQGQIAHYKIPRYI-RFVTE 541 Query: 511 LPRSPNGKLDRKLMQQTLADHF 532 +P + GK+ + +M++ + + Sbjct: 542 MPMTVTGKVQKFVMRERMIEEL 563 >AF2368 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Afu Archaeoglobus fulgidus Length = 562 Score = 190 bits (482), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 149/536 (27%), Positives = 239/536 (44%), Gaps = 40/536 (7%) Query: 7 HDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRME 66 + L +A+ + G Q+ Y ++VA L +G+ G+RV L Sbjct: 44 YRILEMAAEKYGNKVGTIFFGNQITYRQAKEASDKVAGFLFDIGIEKGDRVIFALPNTPH 103 Query: 67 TVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQ 126 + G G + V NPL ++ + +D + + + + ++ L L Sbjct: 104 AAPIMAGILKVGGIIVQCNPLYTQREIKFLAEDSGAKTMFCMDMLYSNVSPLLDDG-TLD 162 Query: 127 TVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSG 186 TV+ P P R W ++A RRA D+A + YT G Sbjct: 163 TVITCSLQDFMVGATMPDPPEKRKGLYSWKNVMEAEKT--ERRAEINPKEDVAMLQYTGG 220 Query: 187 STGQPKGVVLSHRNLVAGAQSVAGYLGNHPQ----DRLLAVLPLSFDYGLSQLSTAFLSG 242 +TG PKGV+L+H N+VA A A + P+ D + +LP+ YG++ ++ L G Sbjct: 221 TTGFPKGVMLTHFNIVANAYQAANW---DPKASSADVGIGMLPVFHSYGMTMMNVGILVG 277 Query: 243 ASVTLLNYLAPRD---ILHAVASEGITGLAGVPPLWQQLAGLPWPQG---CSLRYLTNSG 296 A T++ PR+ +L A+ + +T LA VP ++ + L G SLR T+ Sbjct: 278 A--TIIPMPDPRNYPMVLQAIQTFKVTTLAAVPTMYIGMLKLLEENGYDLSSLRVCTSGA 335 Query: 297 GALPRSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVR 356 LP V + L ++ YGL+EA T+ P + S+G P+ +V+ Sbjct: 336 APLPIEVKRRWEE-LTGKRITEGYGLSEASPITHGNPIYGLNKEGSVGLPYPDTIAVVID 394 Query: 357 PDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVW--SGDS 414 +G+ PGEVGELV RG V +GYWN T + W +GD Sbjct: 395 DEGNILPPGEVGELVIRGPQVMKGYWNRKEETENTL--------------INGWLLTGDM 440 Query: 415 VRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAV-- 472 +MDE G+ Y V R DMI G+ I P E+EEVL+ + AVVG+ P +G+ V Sbjct: 441 AKMDEDGFFYIVDRKKDMIIAGGFNIYPREIEEVLYEHPAVAEAAVVGIPDPYRGETVKA 500 Query: 473 -VAVAPS-SCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQ 526 + + P + E + + C+ L Y +P L + ELP+S GK+ R+++++ Sbjct: 501 FIELKPGWKGKVTEEDIIKFCKERLAPYKVP-KLVEFRDELPKSLVGKVLRRVLRE 555