BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0218.p (187 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO3115 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 159 3e-39 SMb21416 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphory... 157 9e-39 alr2825 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 151 9e-37 STM2092 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 150 2e-36 slr0983 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 148 5e-36 BMEII0827 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphor... 129 5e-30 BS_yfnH [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 127 1e-29 AGl170 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryla... 123 2e-28 SMb21059 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphory... 86 6e-17 >YPO3115 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Ype Yersinia pestis Length = 261 Score = 159 bits (402), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 91/235 (38%), Positives = 126/235 (53%), Gaps = 51/235 (21%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSI 60 M+ IG PILWHIMK Y+S+G NDFV+C GYK +VIK++F NY H D T + + + Sbjct: 29 MVEIGGKPILWHIMKLYSSYGINDFVICCGYKGYVIKEYFANYFMHMSDITFCM-RDNEM 87 Query: 61 HFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDD-FMLTYGDGLGDINL------ 113 H + E PW VTL +TG D+MTG R++ ++ Y+ DD+ F TYGDG+ D+N+ Sbjct: 88 IVHQKRVE-PWNVTLVDTGEDSMTGGRLRRVKDYVKDDEAFCFTYGDGVSDVNIAELIEF 146 Query: 114 ---------------PG---------------SGRPAG-----------LRARTXDYLDQ 132 PG +P G L + D +D Sbjct: 147 HKSHGKQATLTATYPPGRFGALDIKDKQVRSFKEKPKGDGALINGGYFVLSPKVIDLID- 205 Query: 133 DDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 D+ +EQEP+ L A +LM +EH GFWQPMDT R+ L+ L+E+G+APW W Sbjct: 206 GDKSTWEQEPLMTLAAQGELMAFEHAGFWQPMDTLRDKIYLHELWEEGRAPWKVW 260 >SMb21416 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Sme Sinorhizobium meliloti Length = 255 Score = 157 bits (398), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 90/234 (38%), Positives = 125/234 (53%), Gaps = 51/234 (21%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSI 60 MI IG PILWHIMK Y+ +G +DFV+CLGY+ ++IK++F NY H+ D T ++ G Sbjct: 25 MIEIGGRPILWHIMKIYSHYGFSDFVICLGYRGYMIKEYFSNYVLHSSDVTFDMA-TGET 83 Query: 61 HFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDDFMLTYGDGLGDINL------- 113 +HT E PWRVTL +TG ++MTG R+K + Y+ D F LTYGDG+ D+++ Sbjct: 84 AYHTKSAE-PWRVTLVDTGPESMTGGRLKRVAGYL-GDTFCLTYGDGVADVDVRALTEFH 141 Query: 114 --------------PG---------------SGRPAG-----------LRARTXDYLDQD 133 PG + +PAG L D + D Sbjct: 142 LRHGREATVTSVVPPGRYGALATEAGQVMSFTEKPAGDNGRINGGFFVLNRSVLDRIAGD 201 Query: 134 DRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 D + FE P+ L DRQLM + HDGFW+PMDT R+ L L+++ +APW W Sbjct: 202 D-VAFESAPLEGLARDRQLMAFPHDGFWRPMDTLRDKTQLEELWQQNRAPWKIW 254 >alr2825 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Nos Nostoc sp. PCC 7120 Length = 257 Score = 151 bits (381), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 86/236 (36%), Positives = 122/236 (51%), Gaps = 52/236 (22%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSI 60 M+ IG PILWHIMK Y+S G NDF++C GYK +VIK++F NY H D T ++ + + Sbjct: 25 MVEIGGKPILWHIMKTYSSHGINDFIICCGYKGYVIKEYFANYFLHMSDVTFDM-RFNQM 83 Query: 61 HFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDDFMLTYGDGLGDINLP------ 114 + H+ E PWRVTL TG + MTG R+K +++++ +D F TYGDG+ DIN+ Sbjct: 84 NIHSGYAE-PWRVTLVNTGDNTMTGGRLKRVREHLGNDTFCFTYGDGVCDINITELIKFH 142 Query: 115 ---------GSGRPAG----------------------------------LRARTXDYLD 131 + +PAG L D L Sbjct: 143 REQKSLATLTAVQPAGRFGAISLGYEQTKITSFREKPEGDGAWINGGYFILEPEVID-LI 201 Query: 132 QDDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 DD V+E+EP+ KL +L ++H+GFWQPMDT R+ L L++ +APW W Sbjct: 202 ADDATVWEKEPLEKLADMEELSAFKHNGFWQPMDTLRDKNYLEELWKNNQAPWKVW 257 >STM2092 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Sty Salmonella typhimurium LT2 Length = 257 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 87/235 (37%), Positives = 120/235 (51%), Gaps = 51/235 (21%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSI 60 M+ IG PILWHIMK Y+ G DF++C GYK +VIK++F NY H D T + + + Sbjct: 25 MVEIGGKPILWHIMKMYSVHGIKDFIICCGYKGYVIKEYFANYFLHMSDVTFHMAEN-RM 83 Query: 61 HFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDD-FMLTYGDGLGDINLPGS--- 116 H + E PW VTL +TG +MTG R+K + +Y+ DD+ F+ TYGDG+ D+++ + Sbjct: 84 EVHHKRVE-PWNVTLVDTGDSSMTGGRLKRVAEYVKDDEAFLFTYGDGVADLDIKATIDF 142 Query: 117 ----GRPAGLRA----------------------------------------RTXDYLDQ 132 G+ A L A D +D Sbjct: 143 HKAHGKKATLTATFPPGRFGALDIRAGQVRSFQEKPKGDGAMINGGFFVLNPSVIDLID- 201 Query: 133 DDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 +D +EQEP+ L +LM +EH GFWQPMDT R+ L L+EKGKAPW W Sbjct: 202 NDATTWEQEPLMTLAQQGELMAFEHPGFWQPMDTLRDKVYLEGLWEKGKAPWKTW 256 >slr0983 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Syn Synechocystis Length = 256 Score = 148 bits (374), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 87/235 (37%), Positives = 125/235 (53%), Gaps = 51/235 (21%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSI 60 MI IG PILWHI+K Y+++G NDF++C GYK +VIK++F NY H D T ++ I Sbjct: 25 MIDIGGRPILWHILKLYSAYGVNDFIICCGYKGYVIKEYFANYFLHMSDVTFDMVSNEMI 84 Query: 61 HFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDD-FMLTYGDGLGDINL------ 113 H + E PW+VTL +TG +++TG R+K I+ +I++D+ F TYGDGL +IN+ Sbjct: 85 -VHQRKAE-PWKVTLVDTGENSLTGGRLKRIKTFIENDNAFCFTYGDGLANINIQELIAF 142 Query: 114 ---------------PG---------------SGRPAG-----------LRARTXDYLDQ 132 PG + +P G L R D++ Sbjct: 143 HQSHGRLATVTAVVPPGRYGALKLENSKVFGFTEKPPGDGGLINGGFFVLSPRCLDFIGG 202 Query: 133 DDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 D +E EP+ +L +LM +EH GFWQPMDT R+ L L++ G+APW W Sbjct: 203 DMD-SWEGEPLTELSGKGELMAFEHRGFWQPMDTLRDKNHLEELWQSGQAPWKVW 256 >BMEII0827 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Bme Brucella melitensis Length = 295 Score = 129 bits (323), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 83/234 (35%), Positives = 121/234 (51%), Gaps = 55/234 (23%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHT-KDFTI-ELGKKG 58 MIP+G PIL H+M+YY+ +GHNDFVLCLGYK++V+KDFFL T D + + G+ Sbjct: 51 MIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVVKDFFLTIRPQTFADCVVSDGGRNV 110 Query: 59 SIHFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYI-DDDDFMLTYGDGLGDINLP--- 114 + D+D W VTL +TG+ G R+ + + ++ ++D F+ Y DGL D++L Sbjct: 111 QLLEEVDRD---WSVTLLDTGIWRNIGERLWSARSHVMNEDIFLANYSDGLSDVDLDNMT 167 Query: 115 ----GSGRPAGLRA---------------------RTX-------------------DYL 130 SG+ A A RT DY+ Sbjct: 168 QRFRASGKVACFLAVRPPLTYHLADIAEGGDVRAFRTSNTSDIWINGGYFLFLKEIFDYM 227 Query: 131 DQDDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPW 184 + + LV EP +L+A+ QLM Y++DGFW+ MDT R++Q L + EKG PW Sbjct: 228 REGEELVL--EPFSRLIAENQLMAYKYDGFWRSMDTLRDWQTLEDMVEKGDMPW 279 >BS_yfnH [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Bsu Bacillus subtilis Length = 254 Score = 127 bits (320), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 76/230 (33%), Positives = 109/230 (47%), Gaps = 49/230 (21%) Query: 4 IGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHTKDFTIELGKKGSIHFH 63 IG PILWHIMK Y +G N+F+L LGYK IK++FL+Y+ T++ G + Sbjct: 28 IGGKPILWHIMKIYQYYGVNEFILLLGYKGEKIKEYFLDYEWKHNSLTLD-SSTGEVQM- 85 Query: 64 TDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDDFMLTYGDGLGDINL-------PGS 116 Q E+ W++T ETG+D +T RI + YI D+ F+LTYGDGL +INL Sbjct: 86 LGQPET-WKITFLETGVDTLTAGRILQAKDYIGDETFLLTYGDGLANINLFHLISYHQTK 144 Query: 117 GRPAG---------------------------------------LRARTXDYLDQDDRLV 137 G A L + DYL +D + Sbjct: 145 GAAATVTGIDKVSQFGTLTVEDGMAKTFSEKTSSDGIINGGFFVLSPKVFDYLPKDGNTM 204 Query: 138 FEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 FE EP++ L D +L Y H GFW +DT++ +N ++ +G+ W W Sbjct: 205 FEDEPLKNLAKDGELAVYRHYGFWTAIDTYKNLLEVNKMWNQGQQVWKVW 254 >AGl170 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 270 Score = 123 bits (309), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 74/234 (31%), Positives = 118/234 (50%), Gaps = 54/234 (23%) Query: 1 MIPIGRFPILWHIMKYYASFGHNDFVLCLGYKSHVIKDFFLNYDAHT-KDFTIELGKKGS 59 +IP+G PI+ H+M+YYA +GH DF+LCLGYK+HVIK+FFLN + D I + Sbjct: 25 LIPLGSQPIMRHVMQYYARYGHEDFILCLGYKAHVIKEFFLNSRPESYADCVISSPGRVE 84 Query: 60 IHFHTDQDESPWRVTLAETGLDAMTGARIKAIQKYIDDDD-FMLTYGDGLGDINLPG--- 115 + +D WRV+L +TG+ G R+ A++ ++ + F+ Y DGL D++L Sbjct: 85 LLEELPRD---WRVSLIDTGIWRNIGERLWAVRHHLKGEKMFLANYSDGLTDVDLDDMIA 141 Query: 116 ---------------------------SGRPAGLRARTX-----------------DYLD 131 SG+ R+ DY+ Sbjct: 142 QFEKSDKLACFLAVRPPLTYHLAEIDESGKVREFRSSETSDMWINGGYFLMRPEIFDYMR 201 Query: 132 QDDRLVFEQEPMRKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWV 185 + + LV EP +L+++ +LM Y+H+GFW+ MDT R++Q L ++ E+G PW+ Sbjct: 202 EGEELVL--EPFSRLISEDKLMAYKHEGFWRSMDTLRDWQTLEAMVERGDMPWI 253 >SMb21059 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Sme Sinorhizobium meliloti Length = 174 Score = 85.5 bits (210), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 53/164 (32%), Positives = 75/164 (45%), Gaps = 48/164 (29%) Query: 71 WRVTLAETGLDAMTGARIKAIQKYIDDDDFMLTYGDGLGDINLP---------------G 115 W V L ETGL +TG RIK + ++++ FMLT+GDG+ +I+L Sbjct: 11 WSVDLVETGLKTLTGGRIKRLIDWMNNGTFMLTWGDGVSNIDLDELVAFHRSHGKLATMT 70 Query: 116 SGRPAG--------------------------------LRARTXDYLDQDDRLVFEQEPM 143 + RP L DY+ D+ + FE PM Sbjct: 71 AVRPPARYGHIQFDGNRVVEFTEKPQASEGWINGAFFVLEPGVRDYIGGDEDM-FEHAPM 129 Query: 144 RKLVADRQLMTYEHDGFWQPMDTHREYQLLNSLYEKGKAPWVRW 187 ++L +D QLM Y H+ FWQ MDT RE +L +L++ G APW W Sbjct: 130 QRLASDGQLMAYRHETFWQCMDTLREKHILQTLWDSGNAPWKTW 173