BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0028.p (230 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC2408 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 256 3e-68 Cgl2347 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 238 8e-63 RSc1441 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 208 9e-54 AGl656 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 194 9e-50 SMb20577 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subuni... 194 1e-49 PA0153 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 191 8e-49 mlr7208 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 190 2e-48 BMEII0636 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subun... 186 4e-47 CC2409 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 103 3e-22 RSc1442 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 92 9e-19 >CC2408 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Ccr Caulobacter vibrioides Length = 235 Score = 256 bits (654), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 125/217 (57%), Positives = 157/217 (72%), Gaps = 2/217 (0%) Query: 12 PELIYPPYKSTVKRGPTQAPLRVRDEIATGTN-LFASPKLILPHDMDLTKQGRGEPLGEK 70 P ++ PY STV R P Q +R+ + T A P+L+ DLT Q + P G++ Sbjct: 13 PPSLHAPYASTVPRSPRQPLVRIPHTLTETTGPSNAWPRLMGGAMADLTTQHKAMPQGQR 72 Query: 71 IVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTDDNGRY 130 I+V+GRV+DEDG+PV N+++E+WQ NAAGRY HKKDQ DAPLDPNFTG G+++TDD GRY Sbjct: 73 IIVSGRVLDEDGRPVPNTVMEIWQANAAGRYIHKKDQWDAPLDPNFTGAGRVITDDEGRY 132 Query: 131 RFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFAYDPIFQSIPD 190 +FVSI+PG YPWGNHH AWRPAHIH SL G +A RLVTQMYFP+DPL DPI ++P Sbjct: 133 QFVSIRPGAYPWGNHHNAWRPAHIHLSLLGPAFATRLVTQMYFPDDPLIEIDPIANAVP- 191 Query: 191 EAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNATP 227 RQRL+SRF +E T + LGY FD+VLRGR+ TP Sbjct: 192 LPYRQRLVSRFDIEATKPNWALGYLFDVVLRGRDQTP 228 >Cgl2347 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 230 Score = 238 bits (607), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 115/220 (52%), Positives = 152/220 (69%), Gaps = 1/220 (0%) Query: 9 GVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTKQGRGEPLG 68 G Y L +P Y++T+KR P+ + V + T + + D D+TK GE +G Sbjct: 11 GEYSPLHFPEYRTTIKRNPSNDLIMVPSRLGESTGPVFGDRDLGDIDNDMTKVNGGEAIG 70 Query: 69 EKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTDDNG 128 ++I V GRV+ DGKPV ++L+E WQ NAAGRY HK D APLDP+F G + LTD +G Sbjct: 71 QRIFVHGRVLGFDGKPVPHTLVEAWQANAAGRYRHKNDSWPAPLDPHFNGVARTLTDKDG 130 Query: 129 RYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFAYDPIFQSI 188 +Y F ++ PG YPWGNHH AWRPAHIHFSL+G + +RLVTQMYFPNDPLF DPI+ ++ Sbjct: 131 QYHFWTVMPGNYPWGNHHNAWRPAHIHFSLYGRQFTERLVTQMYFPNDPLFFQDPIYNAV 190 Query: 189 PDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNATPM 228 P + AR+R+I+ F +ET ++ LGY+FDIVLRGRNATP Sbjct: 191 P-KGARERMIATFDYDETRENFALGYKFDIVLRGRNATPF 229 >RSc1441 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Rso Ralstonia solanacearum Length = 246 Score = 208 bits (529), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 109/227 (48%), Positives = 145/227 (63%), Gaps = 2/227 (0%) Query: 2 QFDAIEPGVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTN-LFASPKL-ILPHDMDLT 59 +F + ++P + P YK++V R P A + + ++ T +F + L L +D+ L Sbjct: 10 EFAQRDTALHPPALTPGYKTSVLRSPRNALISTLNTLSETTAPVFHADDLGSLDNDLILN 69 Query: 60 KQGRGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGF 119 G P+GE+I+V G V DE G+PV N+L+EVWQ NA GRY HKKDQ+ AP+DPNF G Sbjct: 70 YAKDGLPIGERIIVHGYVRDEFGRPVPNALVEVWQANAGGRYRHKKDQYIAPIDPNFGGC 129 Query: 120 GKMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLF 179 G+MLTD NG Y + +IKPGPYPW N WRP+HIH+SL G+ +AQRL+TQMYF DPL Sbjct: 130 GRMLTDANGYYAYRTIKPGPYPWRNRINDWRPSHIHYSLCGDGWAQRLITQMYFEGDPLI 189 Query: 180 AYDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 A PI ++IP+EA + LI+ V Y FDI LRGR AT Sbjct: 190 AQCPIIRTIPNEAQVRGLIALLDTANHVPLDARCYRFDITLRGRRAT 236 >AGl656 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 246 Score = 194 bits (494), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 102/220 (46%), Positives = 140/220 (63%), Gaps = 3/220 (1%) Query: 10 VYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTKQGR--GE-P 66 ++P P YK+++ R P +A + + + T ++ P D DL GE P Sbjct: 18 IHPLAYAPGYKTSILRSPQRALISLEGTKSEITGPVFGHGVLNPLDNDLILNYARPGEMP 77 Query: 67 LGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTDD 126 +G +I+V GRV+DE G+ V +L+E WQ NA GRY HKK+ + A +DPNF G G+ +TD+ Sbjct: 78 VGPRILVHGRVLDERGRGVDGALVEFWQANAGGRYRHKKESYLAAIDPNFGGVGRTITDE 137 Query: 127 NGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFAYDPIFQ 186 NG Y F +I+PG YPW N WRPAHIHFS+FG+ +AQRL+TQMYF DPL PI + Sbjct: 138 NGYYWFKTIQPGAYPWPNGVNDWRPAHIHFSVFGHGFAQRLITQMYFEGDPLIWKCPIVK 197 Query: 187 SIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 +IPDE A +RLI+ + + ML Y+FDIVLRGR +T Sbjct: 198 TIPDEDAIKRLIAPLDMNAALPMDMLAYKFDIVLRGRRST 237 >SMb20577 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Sme Sinorhizobium meliloti Length = 253 Score = 194 bits (493), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 104/227 (45%), Positives = 137/227 (60%), Gaps = 3/227 (1%) Query: 3 FDAIEPGVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTKQ- 61 F A + +P P YK++V R P +A L + I+ T ++ D DL Sbjct: 18 FFARDRAWHPPAFAPGYKTSVLRSPQKALLSLDGTISEITGPVFGHSMLGELDNDLIHNF 77 Query: 62 -GRGEP-LGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGF 119 GE +GE+I+V GRV+DE G PV L+E WQ NA GRY HKK+ + APLDPNF G Sbjct: 78 ASPGESAIGERIIVHGRVLDERGHPVHGVLVEFWQANAGGRYRHKKESYLAPLDPNFGGC 137 Query: 120 GKMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLF 179 G+ +TD+ GRY F +++PG YPW N WRPAHIHFS+FG+ +AQRL+TQMYF DP+ Sbjct: 138 GRTITDEEGRYWFRTVRPGAYPWPNGVNDWRPAHIHFSVFGHGFAQRLITQMYFEGDPMI 197 Query: 180 AYDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 PI ++PD A ++LI+ TV Y FDIVLRGR +T Sbjct: 198 WKCPIVGTVPDRRAIEQLIAALDWGNTVPMDARAYRFDIVLRGRRST 244 >PA0153 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Pae Pseudomonas aeruginosa Length = 239 Score = 191 bits (486), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 102/218 (46%), Positives = 132/218 (60%), Gaps = 2/218 (0%) Query: 11 YPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTKQ--GRGEPLG 68 +P+ + P YK++V R P+QA + + ++ + S + HD DL G P G Sbjct: 17 HPKALTPDYKTSVPRSPSQALVSIPQSLSETSGPDFSHLRLGRHDNDLLLNFDHGGLPQG 76 Query: 69 EKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTDDNG 128 E+I++ GRV D+ GKPV ++L+E+WQ NA GRY HK D++ APLDPNF G G+ LTD G Sbjct: 77 ERIIMFGRVFDQYGKPVPHTLVEMWQANAGGRYRHKNDRYLAPLDPNFGGVGRTLTDSEG 136 Query: 129 RYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFAYDPIFQSI 188 Y F +IKPGPYPW N WRPAHIH S+ G A RLVTQMYF DPL PI +++ Sbjct: 137 HYYFRTIKPGPYPWRNGPNDWRPAHIHVSVSGPAIAARLVTQMYFEGDPLIPKCPIIRTL 196 Query: 189 PDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 D A Q LI R + L Y FDIVLRG+ T Sbjct: 197 ADPEAAQSLIGRLDMSMANPMDCLAYRFDIVLRGQRKT 234 >mlr7208 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Mlo Mesorhizobium loti Length = 251 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 101/219 (46%), Positives = 135/219 (61%), Gaps = 3/219 (1%) Query: 11 YPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTKQGR--GE-PL 67 +P + P YKS+V R P +A L + ++ ++ D DL GE + Sbjct: 24 HPPALTPGYKSSVLRSPQKALLSFDNSLSEIAGPVFGHAMLGELDADLIHNFARPGESAI 83 Query: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTDDN 127 GE+I+V GRV+DE G V L+E WQ NA GRY HKKD + APLDPNF G G+ +T ++ Sbjct: 84 GERIIVHGRVLDERGVGVPGVLIEFWQANAGGRYRHKKDGYLAPLDPNFGGCGRTITGED 143 Query: 128 GRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFAYDPIFQS 187 G Y F ++KPGPYPW N WRPAHIHFS+FG+ +AQRL+TQMYF DPL PI + Sbjct: 144 GGYAFRTVKPGPYPWPNGPNDWRPAHIHFSVFGHGFAQRLITQMYFEGDPLIWKCPIVRG 203 Query: 188 IPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 I D+AA + L + + T+ L Y+FDIVLRGR ++ Sbjct: 204 IADKAAIEALTAILDTQATIPMDALAYKFDIVLRGRRSS 242 >BMEII0636 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Bme Brucella melitensis Length = 189 Score = 186 bits (472), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 92/176 (52%), Positives = 123/176 (69%), Gaps = 1/176 (0%) Query: 52 LPHDMDLTKQGRGE-PLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDA 110 L +D+ L GE P+G +I+V GRV+DE +PV +LLE WQ NA GRY HKK+ + A Sbjct: 5 LDNDLILNYARPGEMPVGPRILVHGRVLDEGNRPVPGALLEFWQANAGGRYRHKKETYLA 64 Query: 111 PLDPNFTGFGKMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQ 170 +DPNF G G+ +TD+NG Y F +I+PG YPW N WRPAHIHFS+FG+ +AQRL+TQ Sbjct: 65 AIDPNFGGVGRTITDENGYYWFKTIQPGAYPWPNGVNDWRPAHIHFSVFGHGFAQRLITQ 124 Query: 171 MYFPNDPLFAYDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNAT 226 MYF DPL PI ++IPD++A +RLI+ + T+ M ++FDIVLRGR +T Sbjct: 125 MYFEGDPLIWRCPIVKTIPDKSAIERLIAPLDMNATLPMDMRAFKFDIVLRGRRST 180 >CC2409 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Ccr Caulobacter vibrioides Length = 216 Score = 103 bits (258), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 67/186 (36%), Positives = 99/186 (53%), Gaps = 22/186 (11%) Query: 42 TNLFASPKLI-LPHDMDLTKQGRGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGR 100 + + A P+L H + + +GE GE IV+ G V+D DGK V ++++E+WQ +AAGR Sbjct: 47 SEIGARPELFPAEHYVLASPTAKGEVAGEPIVIEGAVLDADGKAVSDAMIEIWQADAAGR 106 Query: 101 YFHKKDQHDAPLDPNFTGFGKMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFG 160 Y D F G+G+ T ++G YRFV+I+PG + +A HI +FG Sbjct: 107 YV------DGSSHSGFIGWGRASTGEDGVYRFVTIRPGRV---DDQQA---PHIAIGVFG 154 Query: 161 NVYAQRLVTQMYFPNDPLFAYDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVL 220 +RL T++YF +D A DPI +P E RQ LI+R + + FDIVL Sbjct: 155 RGVIKRLATRLYFEDDAANAADPILALVPAE-RRQTLIARKVGQ--------AWRFDIVL 205 Query: 221 RGRNAT 226 +G T Sbjct: 206 QGDRET 211 >RSc1442 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Rso Ralstonia solanacearum Length = 216 Score = 92.0 bits (227), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 55/135 (40%), Positives = 73/135 (54%), Gaps = 4/135 (2%) Query: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFGKMLTD-D 126 GE I + GRV D G VR+ L+E+WQ NA G+Y H D+ D P+DP F G+G+ D D Sbjct: 53 GEHIKLEGRVHDGSGTLVRDVLIEIWQANADGKYAHPGDRQDKPIDPAFRGWGRTGADFD 112 Query: 127 NGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDP-LFAYDPIF 185 G YRF +IKPG A + HI LF L T++YF ++ + DP+ Sbjct: 113 TGIYRFETIKPGAV--AGRGAAVQAPHICMVLFARGINLGLHTRVYFSDEAEANSRDPVL 170 Query: 186 QSIPDEAARQRLISR 200 I E RQ LI+R Sbjct: 171 TGIEWEVRRQTLIAR 185