BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0024.p (285 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Z1787 [R] COG1266 Predicted metal-dependent membrane protease Ec... 112 1e-24 ECs1085 [R] COG1266 Predicted metal-dependent membrane protease ... 111 1e-24 VC1074 [R] COG1266 Predicted metal-dependent membrane protease V... 95 2e-19 YPO1140 [R] COG1266 Predicted metal-dependent membrane protease ... 94 2e-19 PA5132 [R] COG1266 Predicted metal-dependent membrane protease P... 84 3e-16 PA2884 [R] COG1266 Predicted metal-dependent membrane protease P... 84 5e-16 >Z1787 [R] COG1266 Predicted metal-dependent membrane protease EcZ Escherichia coli O157:H7 EDL933 Length = 274 Score = 112 bits (279), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 70/218 (32%), Positives = 113/218 (51%), Gaps = 8/218 (3%) Query: 69 HAWQRRSLLVLALLLALLMALHRWPGFINTLVVPPTHITADARPFMLYANFDKGVAGLLL 128 +AW + V +L A+ + H WPGF N +V+ + + P+ +Y NFDK + LL Sbjct: 62 NAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPFLL 121 Query: 129 LALLVPRCHARREWQDVLRLTLLPGL-VTIALVMGLGWLIGLISPNLKWPGFTPPFLAIN 187 + L ++E + + L L +++ L++ L G + P + +P + P F+ N Sbjct: 122 V--LCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILAN 179 Query: 188 LLLTVVAEEAFFRGVLQHRLQQAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVA 247 L +AEE+ FRG +Q RL + ++ALI++A+LFG H GG AT++ Sbjct: 180 LFFVSLAEESLFRGYIQSRLSEV-----TSPLVALIVAALLFGFYHYSGGALLVLFATLS 234 Query: 248 GAGYGWVFQRTGRIEAAIVLHFALNAVHFIGFTYPALA 285 G YG + +GR+ A + HF LN H + FTYP L Sbjct: 235 GVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLK 272 >ECs1085 [R] COG1266 Predicted metal-dependent membrane protease Ecs Escherichia coli O157:H7 Length = 272 Score = 111 bits (278), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 70/218 (32%), Positives = 113/218 (51%), Gaps = 8/218 (3%) Query: 69 HAWQRRSLLVLALLLALLMALHRWPGFINTLVVPPTHITADARPFMLYANFDKGVAGLLL 128 +AW + V +L A+ + H WPGF N +V+ + + P+ +Y NFDK + LL Sbjct: 60 NAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQSTPYTMYFNFDKALVPFLL 119 Query: 129 LALLVPRCHARREWQDVLRLTLLPGL-VTIALVMGLGWLIGLISPNLKWPGFTPPFLAIN 187 + L ++E + + L L +++ L++ L G + P + +P + P F+ N Sbjct: 120 V--LCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGLKPEIHFPEWLPEFILAN 177 Query: 188 LLLTVVAEEAFFRGVLQHRLQQAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVA 247 L +AEE+ FRG +Q RL + ++ALI++A+LFG H GG AT++ Sbjct: 178 LFFVSLAEESLFRGYIQSRLSEV-----TSPLVALIVAALLFGFYHYSGGALLVLFATLS 232 Query: 248 GAGYGWVFQRTGRIEAAIVLHFALNAVHFIGFTYPALA 285 G YG + +GR+ A + HF LN H + FTYP L Sbjct: 233 GVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLK 270 >VC1074 [R] COG1266 Predicted metal-dependent membrane protease Vch Vibrio cholerae Length = 276 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 77/199 (38%), Positives = 103/199 (51%), Gaps = 14/199 (7%) Query: 90 HRWPGFINTLVVPPTHITADARPFMLYANFDKG-VAGLLLLALLVPRCHARREWQDVLRL 148 H PGF N V+ +A + PF LY N DK V LLLA ++ L Sbjct: 88 HWLPGFSNLKVLDKVIASAHSTPFSLYLNLDKPLVFFALLLAFPALLGQSKTANIKATLL 147 Query: 149 TLLP--GLVTIALVMGLGWLIGLISPNLKWPGFTPPFLAINLLLTVVAEEAFFRGVLQHR 206 TL+P L+ IA+ WL G ++ P + F NLL T VAEEA FRG++Q + Sbjct: 148 TLVPLFALLPIAV-----WL-GALAFEWSLPEWWWIFALNNLLFTCVAEEALFRGLIQQK 201 Query: 207 LQQAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVAGAGYGWVFQRTGRIEAAIV 266 QQ + GA+ L++++ LFG AH GG A +AG GYG VF TGR+ ++ Sbjct: 202 AQQQF-----GAIAGLLIASALFGMAHFAGGPLLMIFAALAGLGYGLVFHFTGRLWVSVG 256 Query: 267 LHFALNAVHFIGFTYPALA 285 +HF N H + FTYP LA Sbjct: 257 VHFLFNFAHLLFFTYPMLA 275 >YPO1140 [R] COG1266 Predicted metal-dependent membrane protease Ype Yersinia pestis Length = 272 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 69/194 (35%), Positives = 102/194 (52%), Gaps = 6/194 (3%) Query: 89 LHRWPGFINTLVVPPTHITADARPFMLYANFDKGVAGLLLLALLVPRCHARREWQDVLRL 148 LH PGF N V+ + PF +Y N DK + +LLA L P ++ V R+ Sbjct: 80 LHLVPGFHNLKVLDKVRTGPLSAPFTMYYNLDKALVPFILLACL-PTLFKVKKHPSVGRM 138 Query: 149 TLLPGLVTIALVMGLGWLIGLISPNLKWPGFTPPFLAINLLLTVVAEEAFFRGVLQHRLQ 208 + ++++ ++ L +G + L P + F+ NL +AEEA FRG LQ RL Sbjct: 139 GWVVLILSVPALLLLAVALGGLKIELHTPVWIGSFIIANLFFVCLAEEALFRGYLQQRLG 198 Query: 209 QAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVAGAGYGWVFQRTGRIEAAIVLH 268 Q W G + AL+++A+LFG+AH GG A +AG YG + +GR+ A+ H Sbjct: 199 Q-WLG----SYPALVITALLFGSAHFAGGPLLMLFAALAGVIYGLAWLWSGRLWVAVAFH 253 Query: 269 FALNAVHFIGFTYP 282 FALN +H + FTYP Sbjct: 254 FALNLMHLLFFTYP 267 >PA5132 [R] COG1266 Predicted metal-dependent membrane protease Pae Pseudomonas aeruginosa Length = 272 Score = 84.3 bits (207), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 76/199 (38%), Positives = 101/199 (50%), Gaps = 16/199 (8%) Query: 89 LHRWPGFINTLVVPPTHITADARPFMLYANFDKGVAGLLLLALLVP--RCHAR---REWQ 143 LH PGF N ++ + + PF +Y NFDK + LLLA L R AR R W Sbjct: 80 LHLLPGFANPSILDKAVVGPQSLPFTMYFNFDKALVPFLLLACLPSLFRDEARAPGRPWH 139 Query: 144 DVLRLTLLPGLVTIALVMGLGWLIGLISPNLKWPGFTPPFLAINLLLTVVAEEAFFRGVL 203 +L + +P L+ +A+ +GL + P L P + F+ NL +AEEA FRG L Sbjct: 140 WLLLVAAVPALLLLAVGVGL------LRPELHAPAWLWQFILANLFFVSLAEEALFRGYL 193 Query: 204 QHRLQQAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVAGAGYGWVFQRTGRIEA 263 Q RL Q W G AL L++ LFG AH GG A +AG YG + +GR+ Sbjct: 194 QQRLGQ-WLG----PWPALALASALFGLAHFAGGPLLMLFAGLAGLIYGLAWLWSGRLWV 248 Query: 264 AIVLHFALNAVHFIGFTYP 282 A + HF LN H + FTYP Sbjct: 249 ATLFHFGLNLTHLLLFTYP 267 >PA2884 [R] COG1266 Predicted metal-dependent membrane protease Pae Pseudomonas aeruginosa Length = 254 Score = 83.6 bits (205), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 69/199 (34%), Positives = 106/199 (53%), Gaps = 12/199 (6%) Query: 86 LMALHRWPGFINTLVVPPTHITADARPFMLYANFDKGVAGLLLLALLVPRCHARREWQDV 145 L+A+H PGFI + P +A M++ +DK + L LLA + RR Q + Sbjct: 60 LIAIHYLPGFIPLRLFPWDLASASPPIQMIWLPWDKALVALTLLAWWL-----RRPKQPL 114 Query: 146 --LRLTLLPGLVTIALVMGLGWLIGLISPNLKWPGFTPPFLAINLLLTVVAEEAFFRGVL 203 L +T L +T +V L + S KWP +L +N+ + +AEEAFFRG+L Sbjct: 115 VSLDITALAFCLTFFVVPLLSIMTNAASWQPKWPDAFWWWLVLNVGVVSLAEEAFFRGLL 174 Query: 204 QHRLQQAWQGLRGGAVLALILSAVLFGAAHLGGGLAYAALATVAGAGYGWVFQRTGRIEA 263 Q +L + W G G +I++ + + A HL YA LA +AG GYG V +GR+ Sbjct: 175 QSQLIR-WFGAWPG----IIVATLAYAALHLLVNPVYALLAGIAGLGYGMVLHFSGRLSL 229 Query: 264 AIVLHFALNAVHFIGFTYP 282 A++LH ++N +HF+ +YP Sbjct: 230 AVLLHASINTLHFLLLSYP 248