BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0149.p (411 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1008 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rso ... 579 e-165 mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo ... 435 e-121 AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Ag... 434 e-121 SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme... 434 e-121 CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr C... 390 e-108 RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Ri... 375 e-104 RC1043 Non COG 210 4e-54 >RSc1008 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rso Ralstonia solanacearum Length = 422 Score = 579 bits (1493), Expect = e-165, Method: Compositional matrix adjust. Identities = 274/411 (66%), Positives = 328/411 (79%), Gaps = 4/411 (0%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLYQLHE RA L+PM WA+ +AK F++P+SPL+ P +QR AAGYELLYRL KEYEKP Sbjct: 1 MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKP 60 Query: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATAL--KQPTVLVVAPLSGH 118 +FNI +V +G+ + IVE+ + EKPFCRL+ F++ + + +P VLV APLSGH Sbjct: 61 EFNIRSVRSNGRDIPIVEQTIIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGH 120 Query: 119 HSTLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPD-VHVI 177 HSTLLR+TVR LLQ+H VY+TDW DARMVPV G FHL DY+ Y+++FI +G D +HVI Sbjct: 121 HSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSDYIAYIREFIAHIGADKLHVI 180 Query: 178 SVCQPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTV 237 SVCQPTVPVLAAISLMAS + P +MTMMGGPIDARKSPTAVN LAT K ++WFEN V Sbjct: 181 SVCQPTVPVLAAISLMASDGEKT-PLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNV 239 Query: 238 IYSVPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYD 297 IY+VPANYPG GR+VYPGFLQHAGFVAMNP RH SH+D+Y+ L +GD AE H +FYD Sbjct: 240 IYTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYD 299 Query: 298 EYNAVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISG 357 EYNAVLDM AE+YL+TI+ VFQ+F+LA GTW ++G+ VRPQDIK ALFTIEGELDDISG Sbjct: 300 EYNAVLDMAAEYYLDTIRDVFQEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISG 359 Query: 358 SGQTQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIR 408 SGQT+AA +LCSSIPK RKQH T GHYGIFSGRRWRE V P++ +FIR Sbjct: 360 SGQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREFVYPQLRDFIR 410 >mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo Mesorhizobium loti Length = 425 Score = 435 bits (1118), Expect = e-121, Method: Compositional matrix adjust. Identities = 206/407 (50%), Positives = 280/407 (68%), Gaps = 3/407 (0%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 M YQL+ELN A L P +A+ +S+P++P+AHT + + +AAG EL R ++ Y KP Sbjct: 1 MFYQLYELNHAALQPARLYADAVRMFYSNPLNPIAHTHWGRSVAAGAELFERTTRRYGKP 60 Query: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHS 120 F +D VD KSV + E+ V KPFC L+ F + + A + P +L+VAP+SGH++ Sbjct: 61 AFGLDKTVVDWKSVEVTEKTVWSKPFCNLVRFERAMPAGRK---PDPKLLIVAPMSGHYA 117 Query: 121 TLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR TV A+L DV+ITDW DARMVP+ G+F L DY+ Y+ + LGPD HV++VC Sbjct: 118 TLLRGTVEAMLPYADVHITDWVDARMVPLVDGSFDLDDYIDYIIEMFHALGPDTHVMAVC 177 Query: 181 QPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYS 240 QP+VPVLAA++LM + DP +P +MT+MGGPID R++PTAVN LA EK WF + V+ Sbjct: 178 QPSVPVLAAVALMEAKGDPFVPSTMTLMGGPIDTRRNPTAVNLLAEEKGIDWFRDNVVMR 237 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 P PGFGR+VYPGFLQ +GF++MN RH +H DF+MHL D +AE+HR FYDEY Sbjct: 238 APWPVPGFGREVYPGFLQLSGFMSMNLDRHIIAHKDFFMHLVKNDGDNAEKHRDFYDEYM 297 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQ 360 AV+D+ AEFYL+T+ VF L +G + G + I++VALFT+EGE DDISG GQ Sbjct: 298 AVMDLTAEFYLQTVDTVFVRHALPKGEMKHRGAAIDTSAIRNVALFTVEGENDDISGLGQ 357 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 T+AAQ+LC +IP +K H+ P GHYG+F+G R+R + P+I +FI Sbjct: 358 TKAAQDLCINIPADKKAHYMQPAVGHYGVFNGSRFRSEIVPRIVDFI 404 >AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 418 Score = 434 bits (1116), Expect = e-121, Method: Compositional matrix adjust. Identities = 202/407 (49%), Positives = 285/407 (70%), Gaps = 3/407 (0%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 + Y L+E+N A + P+ A+ + ++P++PL+ T F + + AG+E+ RL++ Y KP Sbjct: 9 VFYHLYEMNHAAMAPLRAGADMMRQACNNPLNPLSSTAFGRSLDAGFEVFERLTRRYVKP 68 Query: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHS 120 +F++ + VDG++V + +E V +PFC L+HF +DL + T P VL+VAP+SGH++ Sbjct: 69 EFDLGSTSVDGQTVVVTDETVWSRPFCSLVHFNRDL---EPTRKPDPKVLLVAPMSGHYA 125 Query: 121 TLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR TV ALL D+YITDW DAR+VP +G F L DY+ YV + +R +GP HV++VC Sbjct: 126 TLLRGTVEALLPSADIYITDWADARIVPATEGTFDLDDYIDYVIEMLRHIGPGAHVVAVC 185 Query: 181 QPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYS 240 QP+VPVLAA+SLM + D P SMT+MGGPID R +PTAVN LAT KP WF + V+ Sbjct: 186 QPSVPVLAAVSLMEADGDTFAPASMTLMGGPIDTRINPTAVNGLATAKPIEWFRDNVVMQ 245 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VP P FGR VYPGFLQ +GF++MN +H +H DFY++L D SAE+HR+FYDEY Sbjct: 246 VPWPQPAFGRYVYPGFLQLSGFMSMNLDKHMTAHKDFYLNLVKNDGDSAEKHREFYDEYL 305 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQ 360 AV+D+ AEFYL+T++ VF D L +G G+ V P I +VALFT+EGE DDISG GQ Sbjct: 306 AVMDLTAEFYLQTVETVFIDHALPKGNMLHRGRAVDPTAIHNVALFTVEGENDDISGVGQ 365 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 T+AA +LC +IP+ ++ H+ P GHYG+F+G R+R+ + P++ +FI Sbjct: 366 TKAAHDLCRNIPEDKRAHYMQPDVGHYGVFNGSRFRKEIVPRMLDFI 412 >SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme Sinorhizobium meliloti Length = 424 Score = 434 bits (1115), Expect = e-121, Method: Compositional matrix adjust. Identities = 208/411 (50%), Positives = 279/411 (67%), Gaps = 3/411 (0%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 M YQL+E+N A + P A+ FS+P++P++HT F + AAG E+ R ++ Y KP Sbjct: 1 MFYQLYEMNHAMMAPWRTAADAMRLAFSNPMNPVSHTYFGRAAAAGLEVFERATRRYGKP 60 Query: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHS 120 +F + +DG+ V + E +V +PFC LIHF + L +A P VL+VAP+SGH++ Sbjct: 61 EFGLPETEIDGQPVPVRERIVWREPFCNLIHFERSLPRDRA---PDPKVLIVAPMSGHYA 117 Query: 121 TLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVC 180 TLLR TV ALL DVYITDW DARMVP+ +G F L DY+ YV + LGPD HVI VC Sbjct: 118 TLLRGTVEALLPHSDVYITDWIDARMVPLSEGTFDLDDYIDYVIQMLHFLGPDAHVIGVC 177 Query: 181 QPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYS 240 QP VPVLAA++LM +A DP+ P +MT+MGGPID R +PTAVN+LA E+P WF + V+ Sbjct: 178 QPAVPVLAAVALMEAAEDPLAPSTMTLMGGPIDTRINPTAVNELAKERPIEWFRDNVVMP 237 Query: 241 VPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYN 300 VP PGF R VYPGFLQ +GF++MN RH +H +F+ HL D +AE+HR FYDEY Sbjct: 238 VPWPQPGFMRMVYPGFLQLSGFMSMNLDRHLIAHKEFFAHLVKNDGDAAEKHRDFYDEYL 297 Query: 301 AVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQ 360 AV+D+ AEFYL+T++VVF L +G G+ V P I+ VAL T+EGE DDISG GQ Sbjct: 298 AVMDLTAEFYLQTVQVVFMQHALPKGEMIHRGKRVDPTAIRRVALLTVEGENDDISGLGQ 357 Query: 361 TQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIRANA 411 T+AAQ +C+ IP + +QH+ P GHYG+F+G R+R + P+I F R ++ Sbjct: 358 TKAAQTICTGIPDSMRQHYMQPDVGHYGVFNGSRFRREIAPRIVAFQRRHS 408 >CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr Caulobacter vibrioides Length = 427 Score = 390 bits (1001), Expect = e-108, Method: Compositional matrix adjust. Identities = 195/410 (47%), Positives = 267/410 (65%), Gaps = 2/410 (0%) Query: 1 MLYQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKP 60 MLY LHE PM A + + P++P A + +R+ AG +L +++ Y KP Sbjct: 8 MLYALHEAAYYASTPMRLAALAARDFWGSPLNPAADSAVGRRMHAGADLFANVTRRYGKP 67 Query: 61 QFNIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLS-ARQATALK-QPTVLVVAPLSGH 118 ++NID V V V + VV E P+ RL+ F +D++ R+A P VL+VAPLSGH Sbjct: 68 KWNIDKVKVGQVDVRVRPTVVWESPWARLVQFDRDMADMRRAGKFSLDPAVLIVAPLSGH 127 Query: 119 HSTLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVIS 178 ++TLLR TV A L +H V+I DW +AR V V +G F HDY+ +V + LGP +V++ Sbjct: 128 YATLLRGTVEAFLPDHAVFIVDWVNAREVSVLEGRFDFHDYIDHVIQMLEVLGPRPNVVA 187 Query: 179 VCQPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVI 238 VCQP PVLAA +LMA N P P SMT MG PIDAR SPT N LA EKP++WF++ +I Sbjct: 188 VCQPGPPVLAAAALMAEQNHPSRPASMTFMGSPIDARLSPTVTNQLAEEKPFTWFQSNMI 247 Query: 239 YSVPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDE 298 Y+VP Y G GR+VYPGF+Q A F++MN RH ++H ++ HL GD SA++H +FYDE Sbjct: 248 YNVPPPYAGAGRRVYPGFVQLASFMSMNADRHQEAHRRYFNHLVSGDGDSADKHLEFYDE 307 Query: 299 YNAVLDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGS 358 Y +VLD+ EFYL+TI +VFQ + L +G G V+P+ I + L T+EGE DDISG Sbjct: 308 YLSVLDLTEEFYLQTIDIVFQQYLLPKGELVHRGTRVKPEVITDIGLMTVEGENDDISGI 367 Query: 359 GQTQAAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFIR 408 GQTQAA LC++IP+ K + P GHYG+F+GRR+RE + P++ +FIR Sbjct: 368 GQTQAAHGLCANIPEDMKLDYVQPSVGHYGVFNGRRFREEIYPRVRKFIR 417 >RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Rickettsia prowazekii Length = 423 Score = 375 bits (964), Expect = e-104, Method: Compositional matrix adjust. Identities = 187/405 (46%), Positives = 263/405 (64%), Gaps = 7/405 (1%) Query: 3 YQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKPQF 62 Y + E RA + PM + +L+ PL F++ I A L R++++Y KP+F Sbjct: 12 YYILEWLRAQIAPMHLGIKILKELYEH--EPLEDNGFARIIHAYLTLCERMTRKYTKPEF 69 Query: 63 NIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHSTL 122 NI ++ K+ I E+V+ +KPFC L HF+K ++ P +L+VAP++GHH+TL Sbjct: 70 NILETIINDKTYNINEQVIFKKPFCELRHFQKIGFKKEL-----PKLLIVAPMAGHHATL 124 Query: 123 LRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVCQP 182 LR TV ALL D+YITDWT+A VP+E G F + DY+ Y+ +FI +G ++H ++VCQP Sbjct: 125 LRSTVHALLPYTDIYITDWTEANYVPLEAGHFDMDDYIDYLIEFINFMGQNIHTMAVCQP 184 Query: 183 TVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVP 242 TVP+LAAISLM+ N P +P SM ++GGPIDARK+PT VN+ A K WF + VP Sbjct: 185 TVPLLAAISLMSENNSPNVPSSMILIGGPIDARKNPTVVNEFALSKSLEWFCEMLTMQVP 244 Query: 243 ANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAV 302 NYPG+GRKVYPGFLQ GF+++N RH SH + + L + D A+ KFYDEY + Sbjct: 245 PNYPGYGRKVYPGFLQLTGFISLNLLRHIDSHLELWQSLLNSDYQKADYIIKFYDEYLSG 304 Query: 303 LDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQ 362 +DMPAEFYL+TI VFQ F+LARG + E + + + I AL IEGELDDI+ GQT+ Sbjct: 305 MDMPAEFYLQTIDEVFQQFSLARGKFISEKRPIDLKHITKCALLGIEGELDDIAAVGQTK 364 Query: 363 AAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 AA +LC +I ++ K++ GHYG+FSG ++++ + P I EFI Sbjct: 365 AALKLCVNISESMKRYHLQNGVGHYGVFSGSKFKQFIVPLIKEFI 409 >RC1043 Non COG Length = 197 Score = 210 bits (535), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 106/204 (51%), Positives = 137/204 (67%), Gaps = 9/204 (4%) Query: 115 LSGHHSTLLRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDV 174 ++GHH+TLLR TV+ LL +YITDWT+A VP+E G F + DY+ YV + I +GP+V Sbjct: 3 MAGHHATLLRTTVQELLLYTGIYITDWTEASYVPLEAGHFDMDDYIDYVMECINFIGPNV 62 Query: 175 HVISVCQPTVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFE 234 H ++VCQPTVP+LAAISLM+ +N P +P SM +MGGPIDARK+PTAVN+ A K WF Sbjct: 63 HTMAVCQPTVPLLAAISLMSESNSPNVPSSMILMGGPIDARKNPTAVNEFAQSKSLEWFC 122 Query: 235 NTVIYSVPANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRK 294 V+ VP N YPGFLQ A F+ +N RH SH + + L + D A+ K Sbjct: 123 QMVM-QVPPN--------YPGFLQLAVFMGLNLCRHIDSHLELWQSLLNSDYKKADHTIK 173 Query: 295 FYDEYNAVLDMPAEFYLETIKVVF 318 FY EY A ++MPAEFYL+TI VF Sbjct: 174 FYYEYLAGMNMPAEFYLQTIDEVF 197