BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0047.p (257 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Rals... 346 3e-95 PA3743 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pae Pseud... 261 8e-70 PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Paste... 261 1e-69 HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemo... 260 2e-69 VC0563 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Vch Vibri... 257 2e-68 NMB0590 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Nme Neis... 254 1e-67 NMA0793 [J] COG0336 tRNA-(guanine-N1)-methyltransferase NmA Neis... 251 1e-66 YPO3293 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Ype Yers... 250 2e-66 trmD [J] COG0336 tRNA-(guanine-N1)-methyltransferase Eco Escheri... 246 3e-65 ZtrmD [J] COG0336 tRNA-(guanine-N1)-methyltransferase EcZ Escher... 246 3e-65 >RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Ralstonia solanacearum Length = 262 Score = 346 bits (888), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 170/247 (68%), Positives = 200/247 (80%), Gaps = 3/247 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 MQFDV++LFPEMF ALT GIT RA +QQ + L+ WNPR+FTTDN+RTVDDRPYGGGPGM Sbjct: 1 MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 VMLAKPL+AA++A ++ Q A V+ LSPQG PLTH RV+ L+Q LLCGRYE Sbjct: 61 VMLAKPLEAALDAIREAQAP---APSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYE 117 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 AIDQRL+D+ VDEEIS+GDFVLSGGE+ AMA++DAV+R LPG L D+ SAV+DSFVNGLL Sbjct: 118 AIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLL 177 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLIIRAREQGLLSKA 240 D PHYTRPE YEG VP +LLGGHHAEI+KWRRQ+AL T KRPDLI AR QGLLS++ Sbjct: 178 DCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRS 237 Query: 241 DEKFLGS 247 DE FL + Sbjct: 238 DEVFLAA 244 >PA3743 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pae Pseudomonas aeruginosa Length = 252 Score = 261 bits (668), Expect = 8e-70, Method: Compositional matrix adjust. Identities = 133/239 (55%), Positives = 171/239 (71%), Gaps = 7/239 (2%) Query: 5 VVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGMVMLA 64 VV++FPEMF A++ GIT RA++Q L WNPR++T D H+TVDDRP+GGGPGMVM Sbjct: 9 VVSIFPEMFRAISDYGITSRAVKQGLLTLTCWNPRDYTEDRHQTVDDRPFGGGPGMVMKI 68 Query: 65 KPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYEAIDQ 124 KPL+ A+ A+Q A G K +VIYLSPQG LT V L+++ +L+ GRYE ID+ Sbjct: 69 KPLEGALADARQ---AAGGRKAKVIYLSPQGRQLTQAGVRELAEEEALILIAGRYEGIDE 125 Query: 125 RLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLLDSPH 184 R +++ VDEE SIGD+VLSGGELPAM L+DAV RLLPGAL + SA EDSF +GLLD PH Sbjct: 126 RFIEEHVDEEWSIGDYVLSGGELPAMVLVDAVTRLLPGALGHADSAEEDSFTDGLLDCPH 185 Query: 185 YTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI----IRAREQGLLSK 239 YTRPEVY + VP +LL G+H I++WR Q+AL T +R DL+ + EQ LL++ Sbjct: 186 YTRPEVYADKRVPEVLLSGNHEHIRRWRLQQALGRTWERRADLLDSRSLSGEEQKLLAE 244 >PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Pasteurella multocida Length = 245 Score = 261 bits (667), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 126/228 (55%), Positives = 167/228 (73%), Gaps = 4/228 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M +++LFPEMF A+T+ G+T RA++Q ++ WNPR+FT D H+TVDDRPYGGGPGM Sbjct: 1 MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI+AA+ +G +VIYLSPQG L V L+Q +LLCGRYE Sbjct: 61 LMMVQPLRDAIQAAKAE---VG-EGAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 ID+RL++ VDEE S+GD+VL+GGELPAM L+DAV R +PG L ASA EDSF GLL Sbjct: 117 GIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVLGKQASADEDSFAEGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI 228 D PHYTRPEV +G VP +L+ G+H EI+KWR +++LE T +RP+L+ Sbjct: 177 DCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELL 224 >HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemophilus influenzae Length = 246 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 124/228 (54%), Positives = 163/228 (71%), Gaps = 4/228 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M V++LFPEMF A+T+ G+T RA++ ++ WNPR+FT D H+TVDDRPYGGGPGM Sbjct: 1 MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI A+ +VIYLSPQG L V L+Q+ +L+CGRYE Sbjct: 61 LMMVQPLRDAIHTAKAAAGE----GAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 ID+RL+ +DEE SIGD+VL+GGELPAM L+DAV R +PG L ASA EDSF +GLL Sbjct: 117 GIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVLGKQASAEEDSFADGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI 228 D PHYTRPEV EG VP +L+ GHH EI+KWR +++L+ T +RP+L+ Sbjct: 177 DCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELL 224 >VC0563 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Vch Vibrio cholerae Length = 247 Score = 257 bits (657), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 124/254 (48%), Positives = 176/254 (69%), Gaps = 8/254 (3%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M +V+LFPEMF ++T G+T +A+++ ++ WNPR+F D RTVDD+PYGGGPGM Sbjct: 1 MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI AA+Q K +VIYLSPQG L + V L+Q+ +L+CGRYE Sbjct: 61 LMMVQPLRDAIHAAKQASPG----KTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 +D+R+++ VDEE SIGDFV++GGELPAM L+D+V R +PG L D ASA EDSF NGLL Sbjct: 117 GVDERIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVLGDFASAEEDSFANGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLIIRAREQGLLSKA 240 D PHYTRPEV +G+ VP++L G+H +I++WR +++L T +RP+L+ E L+ Sbjct: 177 DCPHYTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELL----ENLALTDE 232 Query: 241 DEKFLGSLPQQAAH 254 E+ L ++ H Sbjct: 233 QEQLLTEYIKETRH 246 >NMB0590 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Nme Neisseria meningitidis MC58 Length = 249 Score = 254 bits (649), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 129/252 (51%), Positives = 173/252 (68%), Gaps = 10/252 (3%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M VT+FPEMF ++T+ G+T RA Q W + NPR+F + +DDRP+GGGPGM Sbjct: 1 MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQ-QRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRY 119 +M+A PL AAIE A+ Q QA +VIYLSPQG PLTH++ V L++ +LLCGRY Sbjct: 61 IMMAPPLHAAIEHAKTQSSQA-----AKVIYLSPQGKPLTHQKAVELAELPHLILLCGRY 115 Query: 120 EAIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGL 179 E ID+RLL VDEEISIGDFV+SGGELPAM LMDAV+RL+PG L D SA +DSF +G+ Sbjct: 116 EGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVLGDMQSAEQDSFSSGI 175 Query: 180 LDSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLIIRAREQGLLSK 239 LD PHYT+P ++G VP +L G+H I +WR +++L T +RPDL+ E+ +L Sbjct: 176 LDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLL----EKRVLIP 231 Query: 240 ADEKFLGSLPQQ 251 + + L ++ Q+ Sbjct: 232 KESRLLETIRQE 243 >NMA0793 [J] COG0336 tRNA-(guanine-N1)-methyltransferase NmA Neisseria meningitidis Z2491 Length = 249 Score = 251 bits (640), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 128/252 (50%), Positives = 171/252 (67%), Gaps = 10/252 (3%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M VT+FPEMF ++T+ G+T RA Q W + NPR+F + +DDRP+GGGPGM Sbjct: 1 MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADNRLGYIDDRPFGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQ-QRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRY 119 +M+A PL AAIE A+ Q QA +VIYLSPQG PLTH + L++ +LLCGRY Sbjct: 61 IMMAPPLHAAIEHAKAQSSQA-----AKVIYLSPQGKPLTHPKAAELAELPHLILLCGRY 115 Query: 120 EAIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGL 179 E ID+RLL VDEEISIGDFV+SGGELPAM LMDAV+RL+PG L D SA +DSF +G+ Sbjct: 116 EGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVLGDMQSAEQDSFSSGI 175 Query: 180 LDSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLIIRAREQGLLSK 239 LD PHYT+P ++G VP +L G+H I +WR +++L T +RPDL+ E+ +L Sbjct: 176 LDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLERRPDLL----EKRVLIP 231 Query: 240 ADEKFLGSLPQQ 251 + + L ++ Q+ Sbjct: 232 KESRLLETIRQE 243 >YPO3293 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Ype Yersinia pestis Length = 246 Score = 250 bits (639), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 124/228 (54%), Positives = 164/228 (71%), Gaps = 4/228 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M V++LFPEMF A+T G+T RA++ +Q W+PR+FT D HRTVDDRPYGGGPGM Sbjct: 1 MWIGVISLFPEMFRAITDYGVTGRAVKNGLLSVQCWSPRDFTYDRHRTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI AA+ +VIYLSPQG L + V L+ + +L+CGRYE Sbjct: 61 LMMVQPLREAIHAAKAAAGE----GAKVIYLSPQGRKLDQQGVCELAMNQKMILVCGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 +D+R++ +DEE SIGD+VLSGGELPAM L+D+V R +PG L ASA EDSFV+GLL Sbjct: 117 GVDERVIKTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHHASAEEDSFVDGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI 228 D PHYTRPEV EG VP +LL G+HAEI++WR +++L T +RP+L+ Sbjct: 177 DCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELL 224 >trmD [J] COG0336 tRNA-(guanine-N1)-methyltransferase Eco Escherichia coli K12 Length = 255 Score = 246 bits (629), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 123/228 (53%), Positives = 161/228 (70%), Gaps = 4/228 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M +++LFPEMF A+T G+T RA++ +Q W+PR+FT D HRTVDDRPYGGGPGM Sbjct: 1 MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI AA+ +VIYLSPQG L V L+ + +L+CGRYE Sbjct: 61 LMMVQPLRDAIHAAKAAAGE----GAKVIYLSPQGRKLDQAGVSELATNQKLILVCGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 ID+R++ +DEE SIGD+VLSGGELPAM L+D+V R +PG L ASA EDSF GLL Sbjct: 117 GIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI 228 D PHYTRPEV EG VP +LL G+HAEI++WR +++L T +RP+L+ Sbjct: 177 DCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELL 224 >ZtrmD [J] COG0336 tRNA-(guanine-N1)-methyltransferase EcZ Escherichia coli O157:H7 EDL933 Length = 255 Score = 246 bits (629), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 123/228 (53%), Positives = 161/228 (70%), Gaps = 4/228 (1%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 M +++LFPEMF A+T G+T RA++ +Q W+PR+FT D HRTVDDRPYGGGPGM Sbjct: 1 MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQALGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 +M+ +PL+ AI AA+ +VIYLSPQG L V L+ + +L+CGRYE Sbjct: 61 LMMVQPLRDAIHAAKAAAGE----GAKVIYLSPQGRKLDQAGVSELATNQKLILVCGRYE 116 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 ID+R++ +DEE SIGD+VLSGGELPAM L+D+V R +PG L ASA EDSF GLL Sbjct: 117 GIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLL 176 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLI 228 D PHYTRPEV EG VP +LL G+HAEI++WR +++L T +RP+L+ Sbjct: 177 DCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELL 224