BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0285.p (311 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2716 [U] COG0341 Preprotein translocase subunit SecF Rso Rals... 393 e-109 NMB0608 [U] COG0341 Preprotein translocase subunit SecF Nme Neis... 343 3e-94 NMA0813 [U] COG0341 Preprotein translocase subunit SecF NmA Neis... 343 3e-94 VC0744 [U] COG0341 Preprotein translocase subunit SecF Vch Vibri... 222 1e-57 STM0408 [U] COG0341 Preprotein translocase subunit SecF Sty Salm... 214 1e-55 secF [U] COG0341 Preprotein translocase subunit SecF Eco Escheri... 214 2e-55 ZsecF [U] COG0341 Preprotein translocase subunit SecF EcZ Escher... 214 2e-55 ECs0460 [U] COG0341 Preprotein translocase subunit SecF Ecs Esch... 214 2e-55 HI0239 [U] COG0341 Preprotein translocase subunit SecF Hin Haemo... 212 1e-54 PA3820 [U] COG0341 Preprotein translocase subunit SecF Pae Pseud... 207 2e-53 >RSc2716 [U] COG0341 Preprotein translocase subunit SecF Rso Ralstonia solanacearum Length = 322 Score = 393 bits (1009), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/270 (71%), Positives = 228/270 (84%), Gaps = 7/270 (2%) Query: 1 MEFFRIKKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPAD 60 MEFFRI++DIPFM+HAL FNA+S LTF+AAVFFLF GLH S+EFTGGTVMEVAY + AD Sbjct: 1 MEFFRIRRDIPFMKHALAFNAVSFLTFLAAVFFLFHNGLHLSVEFTGGTVMEVAYTQAAD 60 Query: 61 LEKIRKSIEGLGYRDTLVQSFGTAQDVMIRLPAQAG-----VNASQQTEKVNAALKADDP 115 L K+R +E LGY D VQ+FGT+ DVMIRLP + G + ++ Q+++V ++L A P Sbjct: 61 LPKVRADVEKLGYADAQVQNFGTSHDVMIRLPLKNGPDGKPIASAVQSQQVMSSLIATTP 120 Query: 116 DVRLQRVEFVGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIII 175 DV+LQRVEFVGPQVG ELA DGLLAL VV+GIVIYL+ RFEWKFAVA +IANLHDI+II Sbjct: 121 DVKLQRVEFVGPQVGKELATDGLLALLFVVIGIVIYLSIRFEWKFAVAGIIANLHDIVII 180 Query: 176 LGFFAFFQWEFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAIT 235 LGFFAFFQWEFSL+VLAA+LAVLGYSVNESVV+FDR+REAFR R K++ EV++HAIT Sbjct: 181 LGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVIFDRIREAFRKYR--KMNTHEVIDHAIT 238 Query: 236 STMSRTVITHGSTEMMVLAMFFFGGPTLHY 265 ST+SRT+ITHGSTEMMVL+M FGGPTLHY Sbjct: 239 STISRTIITHGSTEMMVLSMLVFGGPTLHY 268 >NMB0608 [U] COG0341 Preprotein translocase subunit SecF Nme Neisseria meningitidis MC58 Length = 311 Score = 343 bits (880), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 163/264 (61%), Positives = 210/264 (79%) Query: 1 MEFFRIKKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPAD 60 ME F+IK+DIPFM + + IS +TF+AAVFFL ++GL+FS+EFTGGTVMEV Y + AD Sbjct: 1 MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVEFTGGTVMEVQYQQGAD 60 Query: 61 LEKIRKSIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQQTEKVNAALKADDPDVRLQ 120 + K+R+ ++ L D VQ+ GT + +MIRLP + GV ++Q + +V LK D PDV L+ Sbjct: 61 VNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQLSNQVMDLLKKDSPDVTLR 120 Query: 121 RVEFVGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFA 180 +VEF+GPQVG+EL +GL+ALG VV+GI+IYL+ RFEW+FAV+A+IAN+HDI+IILG FA Sbjct: 121 QVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRFAVSAIIANMHDIVIILGCFA 180 Query: 181 FFQWEFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSR 240 FFQWEFSLTVLA ILAVLGYSVNESVVVFDR+RE FR + PEV+++AIT+TMSR Sbjct: 181 FFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRKPAMRGHAVPEVIDNAITATMSR 240 Query: 241 TVITHGSTEMMVLAMFFFGGPTLH 264 T+ITHGSTE MV++M FGG LH Sbjct: 241 TIITHGSTEAMVVSMLVFGGAALH 264 >NMA0813 [U] COG0341 Preprotein translocase subunit SecF NmA Neisseria meningitidis Z2491 Length = 311 Score = 343 bits (880), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 163/264 (61%), Positives = 210/264 (79%) Query: 1 MEFFRIKKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPAD 60 ME F+IK+DIPFM + + IS +TF+AAVFFL ++GL+FS+EFTGGTVMEV Y + AD Sbjct: 1 MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVEFTGGTVMEVQYQQGAD 60 Query: 61 LEKIRKSIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQQTEKVNAALKADDPDVRLQ 120 + K+R+ ++ L D VQ+ GT + +MIRLP + GV ++Q + +V LK D PDV L+ Sbjct: 61 VNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQLSNQVMDLLKKDSPDVTLR 120 Query: 121 RVEFVGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFA 180 +VEF+GPQVG+EL +GL+ALG VV+GI+IYL+ RFEW+FAV+A+IAN+HDI+IILG FA Sbjct: 121 QVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRFAVSAIIANMHDIVIILGCFA 180 Query: 181 FFQWEFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSR 240 FFQWEFSLTVLA ILAVLGYSVNESVVVFDR+RE FR + PEV+++AIT+TMSR Sbjct: 181 FFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRKPAMRGHAVPEVIDNAITATMSR 240 Query: 241 TVITHGSTEMMVLAMFFFGGPTLH 264 T+ITHGSTE MV++M FGG LH Sbjct: 241 TIITHGSTEAMVVSMLVFGGAALH 264 >VC0744 [U] COG0341 Preprotein translocase subunit SecF Vch Vibrio cholerae Length = 315 Score = 222 bits (565), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 116/264 (43%), Positives = 180/264 (68%), Gaps = 3/264 (1%) Query: 2 EFFRIKKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADL 61 + + K I FMR + A+S + A++F L +K L++ ++FTGGT++EV + +PA+L Sbjct: 3 QILKADKMIDFMRWSKFAFALSLVMIAASIFTLSTKWLNWGLDFTGGTLIEVGFEQPANL 62 Query: 62 EKIRKSIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQQTEKVNAALK-ADDPDVRLQ 120 E+IR ++E G+ D VQ+FG+A+DVM+RL + + ++ AA+K +V ++ Sbjct: 63 EQIRSALEAKGFGDATVQNFGSARDVMVRLRPRDDMAGEALGNQILAAIKEGTGGNVEMR 122 Query: 121 RVEFVGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFA 180 R+EFVGP VGDEL G LA+ + +L I++Y++ RFEW+ A AV+A HD+II LG F+ Sbjct: 123 RIEFVGPNVGDELTEAGGLAILVSLLCILLYVSVRFEWRLAAGAVLALAHDVIITLGIFS 182 Query: 181 FFQWEFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSR 240 Q E LT++AA+L V+GYS+N+++VVFDR+RE FR R K P E++N +IT T+SR Sbjct: 183 ILQIEVDLTIVAALLTVVGYSLNDTIVVFDRIRENFRKMR--KEEPAEIMNSSITQTLSR 240 Query: 241 TVITHGSTEMMVLAMFFFGGPTLH 264 T+IT G+T +V+A+F GG +H Sbjct: 241 TLITSGTTLFVVIALFTQGGAMIH 264 >STM0408 [U] COG0341 Preprotein translocase subunit SecF Sty Salmonella typhimurium LT2 Length = 323 Score = 214 bits (546), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 102/260 (39%), Positives = 171/260 (65%), Gaps = 4/260 (1%) Query: 7 KKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADLEKIRK 66 +K FMR IS L +AA+ + +G ++ ++FTGGTV+E+ KPA+++ +R+ Sbjct: 14 RKVYDFMRWDFWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEKPAEMDVMRE 73 Query: 67 SIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQ--QTEKVNAALKADDPDVRLQRVEF 124 +++ GY + +Q+FG++ D+M+R+P G Q ++ V +A + + ++R+EF Sbjct: 74 ALQKAGYEEPQLQNFGSSHDIMVRMPPTEGETGGQVLGSKVVTIINEATNQNAAVKRIEF 133 Query: 125 VGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQW 184 VGP VG +LA G +AL + ++ I++Y+ FRFEW+ A VIA HD+II LG + F Sbjct: 134 VGPSVGADLAQTGAMALLVALISILVYVGFRFEWRLAAGVVIALAHDVIITLGILSLFHI 193 Query: 185 EFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVIT 244 E LT++A++++V+GYS+N+S+VV DR+RE FR R G +P E+ N ++T T+ RT+IT Sbjct: 194 EIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRG--TPYEIFNVSLTQTLHRTLIT 251 Query: 245 HGSTEMMVLAMFFFGGPTLH 264 G+T +++L ++ FGGP L Sbjct: 252 SGTTLVVILMLYLFGGPVLE 271 >secF [U] COG0341 Preprotein translocase subunit SecF Eco Escherichia coli K12 Length = 323 Score = 214 bits (545), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 101/260 (38%), Positives = 170/260 (65%), Gaps = 4/260 (1%) Query: 7 KKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADLEKIRK 66 +K FMR IS L +AA+ + +G ++ ++FTGGTV+E+ KPA+++ +R Sbjct: 14 RKVYDFMRWDYWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEKPAEIDVMRD 73 Query: 67 SIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQ--QTEKVNAALKADDPDVRLQRVEF 124 +++ G+ + ++Q+FG++ D+M+R+P G Q ++ + ++ + + ++R+EF Sbjct: 74 ALQKAGFEEPMLQNFGSSHDIMVRMPPAEGETGGQVLGSQVLKVINESTNQNAAVKRIEF 133 Query: 125 VGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQW 184 VGP VG +LA G +AL +L I++Y+ FRFEW+ A VIA HD+II LG + F Sbjct: 134 VGPSVGADLAQTGAMALMAALLSILVYVGFRFEWRLAAGVVIALAHDVIITLGILSLFHI 193 Query: 185 EFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVIT 244 E LT++A++++V+GYS+N+S+VV DR+RE FR R G +P E+ N ++T T+ RT+IT Sbjct: 194 EIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRG--TPYEIFNVSLTQTLHRTLIT 251 Query: 245 HGSTEMMVLAMFFFGGPTLH 264 G+T M++L ++ FGGP L Sbjct: 252 SGTTLMVILMLYLFGGPVLE 271 >ZsecF [U] COG0341 Preprotein translocase subunit SecF EcZ Escherichia coli O157:H7 EDL933 Length = 323 Score = 214 bits (545), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 101/260 (38%), Positives = 170/260 (65%), Gaps = 4/260 (1%) Query: 7 KKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADLEKIRK 66 +K FMR IS L +AA+ + +G ++ ++FTGGTV+E+ KPA+++ +R Sbjct: 14 RKVYDFMRWDYWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEKPAEIDVMRD 73 Query: 67 SIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQ--QTEKVNAALKADDPDVRLQRVEF 124 +++ G+ + ++Q+FG++ D+M+R+P G Q ++ + ++ + + ++R+EF Sbjct: 74 ALQKAGFEEPMLQNFGSSHDIMVRMPPAEGETGGQVLGSQVLKVINESTNQNAAVKRIEF 133 Query: 125 VGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQW 184 VGP VG +LA G +AL +L I++Y+ FRFEW+ A VIA HD+II LG + F Sbjct: 134 VGPSVGADLAQTGAMALMAALLSILVYVGFRFEWRLAAGVVIALAHDVIITLGILSLFHI 193 Query: 185 EFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVIT 244 E LT++A++++V+GYS+N+S+VV DR+RE FR R G +P E+ N ++T T+ RT+IT Sbjct: 194 EIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRG--TPYEIFNVSLTQTLHRTLIT 251 Query: 245 HGSTEMMVLAMFFFGGPTLH 264 G+T M++L ++ FGGP L Sbjct: 252 SGTTLMVILMLYLFGGPVLE 271 >ECs0460 [U] COG0341 Preprotein translocase subunit SecF Ecs Escherichia coli O157:H7 Length = 323 Score = 214 bits (545), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 101/260 (38%), Positives = 170/260 (65%), Gaps = 4/260 (1%) Query: 7 KKDIPFMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADLEKIRK 66 +K FMR IS L +AA+ + +G ++ ++FTGGTV+E+ KPA+++ +R Sbjct: 14 RKVYDFMRWDYWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEKPAEIDVMRD 73 Query: 67 SIEGLGYRDTLVQSFGTAQDVMIRLPAQAGVNASQ--QTEKVNAALKADDPDVRLQRVEF 124 +++ G+ + ++Q+FG++ D+M+R+P G Q ++ + ++ + + ++R+EF Sbjct: 74 ALQKAGFEEPMLQNFGSSHDIMVRMPPAEGETGGQVLGSQVLKVINESTNQNAAVKRIEF 133 Query: 125 VGPQVGDELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQW 184 VGP VG +LA G +AL +L I++Y+ FRFEW+ A VIA HD+II LG + F Sbjct: 134 VGPSVGADLAQTGAMALMAALLSILVYVGFRFEWRLAAGVVIALAHDVIITLGILSLFHI 193 Query: 185 EFSLTVLAAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVIT 244 E LT++A++++V+GYS+N+S+VV DR+RE FR R G +P E+ N ++T T+ RT+IT Sbjct: 194 EIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRG--TPYEIFNVSLTQTLHRTLIT 251 Query: 245 HGSTEMMVLAMFFFGGPTLH 264 G+T M++L ++ FGGP L Sbjct: 252 SGTTLMVILMLYLFGGPVLE 271 >HI0239 [U] COG0341 Preprotein translocase subunit SecF Hin Haemophilus influenzae Length = 325 Score = 212 bits (539), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 107/253 (42%), Positives = 169/253 (66%), Gaps = 4/253 (1%) Query: 12 FMRHALIFNAISALTFVAAVFFLFSKGLHFSIEFTGGTVMEVAYNKPADLEKIRKSIEGL 71 FM+ + +SAL V ++FF+ +KG ++ ++FTGG V + +++ A+LE+IR + Sbjct: 29 FMKVRKLGYILSALLMVISLFFIITKGFNWGLDFTGGVVFDTHFSQSANLEQIRSKLHEN 88 Query: 72 GYRDTLVQSFGTAQDVMIRLPAQAGVNASQQTEKVNAALKADDPDVRLQRVEFVGPQVGD 131 G +VQ+ G+ QDVMIRLPA N S E V + L+ D D++++ +EFVGP VG+ Sbjct: 89 GIESPIVQTTGSVQDVMIRLPASN--NDSTIGEHVKSMLQNVDKDIQIRSIEFVGPNVGE 146 Query: 132 ELAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQWEFSLTVL 191 ELA + A + ++IY+ RFEW+ ++ + HD+II LG F+ Q E LT + Sbjct: 147 ELAQGAVYATLATLAMVLIYVGSRFEWRLGFGSIASLAHDVIITLGVFSALQIEIDLTFV 206 Query: 192 AAILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVITHGSTEMM 251 AAIL+V+GYS+N+S+VVFDRVRE FR R +L ++++ ++T T+SRT+IT +T ++ Sbjct: 207 AAILSVVGYSINDSIVVFDRVRENFRKIR--RLDTIDIIDISLTQTLSRTIITSVTTLVV 264 Query: 252 VLAMFFFGGPTLH 264 V+A+FFFGGP++H Sbjct: 265 VMALFFFGGPSIH 277 >PA3820 [U] COG0341 Preprotein translocase subunit SecF Pae Pseudomonas aeruginosa Length = 306 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 115/251 (45%), Positives = 170/251 (67%), Gaps = 11/251 (4%) Query: 18 IFNAISALTFVAAVFFL---FSKGLHFSIEFTGGTVMEVAYNKPADLEKIRKSIEGLGYR 74 I N A+T + V L F+KG++F ++FTGGT++E+ Y +PADL K+R + G GY Sbjct: 13 IRNVAFAVTLILTVIALGSWFTKGINFGLDFTGGTLIELTYEQPADLGKVRGQLVGAGYE 72 Query: 75 DTLVQSFGTAQDVMIRLPAQAGVNASQQTEKVNAALKADDPD--VRLQRVEFVGPQVGDE 132 D +VQSFG A+DV++R+P++ + +KV AL+ D L+RVE+VGPQVG+E Sbjct: 73 DAVVQSFGDARDVLVRMPSED----PELGKKVATALQQADAGNPANLKRVEYVGPQVGEE 128 Query: 133 LAHDGLLALGMVVLGIVIYLAFRFEWKFAVAAVIANLHDIIIILGFFAFFQWEFSLTVLA 192 L G L + + + GI++Y+ FRF+WKFA+ A+++ +HD II++G +FFQ F LTVLA Sbjct: 129 LRDQGGLGMLLALGGILLYVGFRFQWKFALGAILSLVHDAIIVMGVLSFFQVTFDLTVLA 188 Query: 193 AILAVLGYSVNESVVVFDRVREAFRDRRFGKLSPPEVLNHAITSTMSRTVITHGSTEMMV 252 A+LAV+GYS+N+++V+FDRVRE FR R K E LN + + T+ RT+ T ST + + Sbjct: 189 AVLAVVGYSLNDTIVIFDRVRENFRVLR--KADLVENLNISTSQTLLRTIATSVSTLLAI 246 Query: 253 LAMFFFGGPTL 263 A+ FFGG L Sbjct: 247 AALLFFGGDNL 257