BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0220.p (257 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2515 [IQR] COG1028 Dehydrogenases with different specificities... 292 5e-79 Cgl2355 [IQR] COG1028 Dehydrogenases with different specificitie... 259 6e-69 SA2260 [IQR] COG1028 Dehydrogenases with different specificities... 110 4e-24 mll6713 [IQR] COG1028 Dehydrogenases with different specificitie... 108 9e-24 TM0325 [IQR] COG1028 Dehydrogenases with different specificities... 107 2e-23 mlr6366 [IQR] COG1028 Dehydrogenases with different specificitie... 106 5e-23 SSO2289 [IQR] COG1028 Dehydrogenases with different specificitie... 103 5e-22 SMa0187 [IQR] COG1028 Dehydrogenases with different specificitie... 102 6e-22 SMc02041 [IQR] COG1028 Dehydrogenases with different specificiti... 102 8e-22 AGc3482 [IQR] COG1028 Dehydrogenases with different specificitie... 102 1e-21 >PA2515 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 253 Score = 292 bits (747), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 145/257 (56%), Positives = 184/257 (71%), Gaps = 4/257 (1%) Query: 1 MKSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVA 60 M RF KV +V+GAAQGI GVA + EEG +V+ VDR+E VH++A +A L A Sbjct: 1 MNDRFRDKVALVSGAAQGIGLGVARRLLEEGARVVAVDRSELVHELAGDAC----LCLTA 56 Query: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG 120 DLE YA Q ++ ++R+GR+DIL+N VGG + +PY Y +I+AE+RRSL+PTL+ Sbjct: 57 DLERYAECQRVLERTLERFGRLDILVNNVGGTLWAKPYQHYAEDEIEAELRRSLLPTLWC 116 Query: 121 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 C A LP +L QG G IVNVSS ATRG+ RVPY AAKGGVNALTA LA E + GIRV A Sbjct: 117 CRAALPAMLGQGSGAIVNVSSVATRGVNRVPYGAAKGGVNALTACLAFETADQGIRVNAV 176 Query: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 APGGT APPRR+PRN S++E+ W ++ V+Q S+ KRY +++EQ+A ILFLASD+ Sbjct: 177 APGGTDAPPRRIPRNAAAQSEEERRWYRQIVEQTLDSSLMKRYGSIDEQVAAILFLASDE 236 Query: 241 ASYITGAVLPVAGGDNG 257 ASYITG LPVAGGD G Sbjct: 237 ASYITGVTLPVAGGDLG 253 >Cgl2355 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cgl Corynebacterium glutamicum Length = 276 Score = 259 bits (661), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 139/257 (54%), Positives = 174/257 (67%), Gaps = 3/257 (1%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAE---AAGGRGKGLVA 60 RF G+ VVVTGAAQGI VA IA E G ++LVDR+ VH+VA E A G +A Sbjct: 20 RFFGQGVVVTGAAQGIGMAVAHRIAYEDGNLVLVDRSPLVHEVAEELRKAGAGTVDSFIA 79 Query: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG 120 DLE++ GA A++ A Q+ +D++IN VGG I +PY +Y+ +I EI RSL PTL+ Sbjct: 80 DLETFEGATDALEFAGQKLKNLDVVINNVGGTIWAKPYQEYSEEEIRKEINRSLFPTLWM 139 Query: 121 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 C A LP L+ G G IVNVSS AT GI RVPY+AAKGGVN + +A+A E +RVVAT Sbjct: 140 CRAALPILIGNGGGTIVNVSSIATGGINRVPYAAAKGGVNGIVSAMAREAAPHNVRVVAT 199 Query: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 APGGT AP R V R P + + E+ W Q+ V Q S+ KRY TLEEQ+API FLAS++ Sbjct: 200 APGGTLAPERAVKRGPGPEGELEEKWYQQIVDQTIDSSLMKRYGTLEEQVAPICFLASEE 259 Query: 241 ASYITGAVLPVAGGDNG 257 ASYITG+V+PV GGD G Sbjct: 260 ASYITGSVMPVGGGDQG 276 >SA2260 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sau Staphylococcus aureus N315 Length = 272 Score = 110 bits (274), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 87/265 (32%), Positives = 130/265 (49%), Gaps = 30/265 (11%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAE--AAGGRGKGLVAD 61 R KV VVTGA+ GI + A+ +A+EG VL VD AE V + + + G K D Sbjct: 3 RLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSETVDKIKSNGDNAKAYNVD 62 Query: 62 LESYAGAQAAMDEAVQRYGRIDILINGVG-----GAIRMRPYAQYTPTQIDAEIRRSLMP 116 + + + +++GRID+L N G G I P Y ++ ++R +++ Sbjct: 63 ISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEYPIDVYDKI-MNVDMRGTILM 121 Query: 117 TLYGCHAVLPQLLQQGRGVIVNVSSNA--TRGIRRVPYSAAKGGVNALTAALAMEYGELG 174 T +LP ++ QG G IVN SS + + R Y+AAKG V T ++A+EYG G Sbjct: 122 T----KMMLPLMMNQG-GSIVNTSSFSGQAEDLYRSGYNAAKGAVINFTKSIAIEYGRDG 176 Query: 175 IRVVATAPGGTQAP-PRRVPRNPEGDS----QQEQMWMQEAVQQVTGSNYFKRYSTLEEQ 229 IR A APG + P ++ E D+ ++ Q WM R EE Sbjct: 177 IRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTP----------LGRLGKPEEV 226 Query: 230 IAPILFLASDDASYITGAVLPVAGG 254 ++FLASDD+S+ITG + + GG Sbjct: 227 AKLVVFLASDDSSFITGETIRIDGG 251 >mll6713 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 253 Score = 108 bits (271), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 84/254 (33%), Positives = 121/254 (47%), Gaps = 13/254 (5%) Query: 8 KVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAE---AAGGRGKGLVADLES 64 + +VTGA GI R AL IA EG V + DR+ + + AAGGR K LV DL Sbjct: 6 RTAIVTGAGSGIGRAAALIIAREGAMVGVADRSADGANATVDQILAAGGRAKALVFDLTD 65 Query: 65 YAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGCHAV 124 +A + + GRIDIL N G + + D ++ G V Sbjct: 66 DQALEAGFHDFIHTQGRIDILHNHAGVQVEGDVLG-VSVKGFDTSWTLNVRAHFLGARIV 124 Query: 125 LPQLLQQGRGVIVNVSSNATRGIRR--VPYSAAKGGVNALTAALAMEYGELGIRVVATAP 182 +P + + GRGVI+N SS++ R + Y+ K V A+T +A +YG GIRV A P Sbjct: 125 MPFMKKAGRGVILNTSSSSGVLYDREMIAYTTTKHAVIAMTRQMAGDYGRFGIRVNALCP 184 Query: 183 GGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDDAS 242 G P G + + +++E V R++T+EE +LFL SD +S Sbjct: 185 GWVDTPFNAPFIAQMGGREAIEAYIRERVP-------LGRWATVEEIAEAVLFLVSDRSS 237 Query: 243 YITGAVLPVAGGDN 256 Y+TG +L V GG+ Sbjct: 238 YMTGQILVVDGGET 251 >TM0325 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 251 Score = 107 bits (267), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 89/263 (33%), Positives = 135/263 (51%), Gaps = 29/263 (11%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAE---AAGGRGKGLVA 60 F GKVV++TGA GI + A+ AE G +V + D +E E + GG + Sbjct: 2 NFQGKVVLITGAGSGIGKKAAVMFAERGAKVAINDISEEKGKETVELIKSMGGEAAFIFG 61 Query: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLM----- 115 D+ A+ + + V+ +GR+DIL+N G + PY T + + +++ Sbjct: 62 DVAK--DAEQIVKKTVETFGRLDILVNNAG----IVPYGNIEETS-EEDFDKTMAVNVKG 114 Query: 116 PTLYGCHAVLPQLLQQGRGVIVNVSSNA-TRGI-RRVPYSAAKGGVNALTAALAMEYGEL 173 P L +AV Q+ +QG GVIVNVSS A GI RR YS +K + LT +LA++Y + Sbjct: 115 PFLLSKYAV-EQMKKQGGGVIVNVSSEAGLIGIPRRCVYSVSKAALLGLTRSLAVDYVDY 173 Query: 174 GIRVVATAPGGTQAP--PRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIA 231 GIRV A PG TQ+ RV +P +E ++++T KR EE Sbjct: 174 GIRVNAVCPGTTQSEGLMARVKASPNP---------EELLKKMTSRIPMKRLGKEEEIAF 224 Query: 232 PILFLASDDASYITGAVLPVAGG 254 ILF A D+A ++TG+++ + GG Sbjct: 225 AILFAACDEAGFMTGSIINIDGG 247 >mlr6366 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 374 Score = 106 bits (264), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 85/266 (31%), Positives = 129/266 (48%), Gaps = 34/266 (12%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVD-----RAEFVHDVAAEAAGGRGKGL 58 RF GKV VVTGA GI + AL IA EGG+V++ D +AAEA G L Sbjct: 96 RFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADIDGSAAVACTAQIAAEA--GHALAL 153 Query: 59 VADLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMP-- 116 D+ A + A + +G +D+L+N A+ + P T +D + M Sbjct: 154 AMDIADAQAVAALFETAERHFGGVDLLVNN-ASAMHLTPR-DRTILDLDLAVWDQTMATN 211 Query: 117 ---TLYGCHAVLPQLLQQGRGVIVNVSSNATRGIR----RVPYSAAKGGVNALTAALAME 169 TL C +P+++ +G G IVN+SS +G+ + Y+A+K +N L+A+LA + Sbjct: 212 LRGTLLCCRQAIPRMIARGGGAIVNMSS--CQGLSGDTAQTSYAASKAAMNMLSASLATQ 269 Query: 170 YGELGIRVVATAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQ-QVTGSNYFKRYSTLEE 228 YG IR A APG +++ + E +Q ++ +R E+ Sbjct: 270 YGHAQIRCNAVAPGLIM-------------TERLLAKLDECMQRHLSRHQLLQRVGRPED 316 Query: 229 QIAPILFLASDDASYITGAVLPVAGG 254 A + FL SDDA++ITG VL + GG Sbjct: 317 VAALVAFLLSDDAAFITGQVLCIDGG 342 >SSO2289 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sso Sulfolobus solfataricus Length = 259 Score = 103 bits (256), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 81/263 (30%), Positives = 127/263 (48%), Gaps = 26/263 (9%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQ-VLLVDRAEFVHDVAAEAAGGRGKGLV--- 59 RF KV+++TG +GI R + EGG ++L + AE EA R KG+ Sbjct: 8 RFKDKVILITGGTRGIGRAITEAFLREGGLPIVLYNSAE------DEAKKLREKGVFTIK 61 Query: 60 ADLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLY 119 D+ + + + + + + R+++++N G + M P+ ++ + + IR +L T+Y Sbjct: 62 CDVGNREDVKKSKEIVEKEFRRVNVVVNNAGIMLTM-PFEEFDEEKYNKMIRINLNGTIY 120 Query: 120 GCHAVLPQLLQQGRGVIVNVSSNATRGIR---RVPYSAAKGGVNALTAALAMEYGELGIR 176 + LP L G IVN++SNA G Y+ K + LT LA E G+ GIR Sbjct: 121 TTYEFLPLLKASENGAIVNIASNAGIGTAAEGTTFYAITKASIIILTKRLAFELGKYGIR 180 Query: 177 VVATAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPI-LF 235 V A APG + ++ E + ++ + V + TG E IA I LF Sbjct: 181 VNAVAPGWVETDMTLSGKSKEEVERLRDLFRNKTVLKTTGK---------PEDIANIVLF 231 Query: 236 LASDDASYITGAVLPVAGG--DN 256 LAS++A YITG V+ GG DN Sbjct: 232 LASNEARYITGQVIVADGGRIDN 254 >SMa0187 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 256 Score = 102 bits (255), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 83/258 (32%), Positives = 121/258 (46%), Gaps = 19/258 (7%) Query: 3 SRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLV--DRAEFVHDV--AAEAAGGRGKGL 58 + KV ++TG GI R VA+ A EG V +V D ++ D A E G + + Sbjct: 11 DKLKDKVALITGGDSGIGRSVAVLFAREGADVAIVHLDESQDADDTKAAVEKEGRKCLVI 70 Query: 59 VADLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTL 118 D++ + + A+++ V + GR+DILIN + R T D ++ +L Sbjct: 71 KGDVKDASFCRKAVEKTVMQLGRLDILINNAAFQVHTRDIEDLTDEHFDETLKTNLYGYF 130 Query: 119 YGCHAVLPQLLQQGRGVIVNVSSNATRGIRR-VPYSAAKGGVNALTAALAMEYGELGIRV 177 Y A +P L+ G +I S G + + YS KGG++A T AL+ GIRV Sbjct: 131 YMAKAAIPH-LKNGSAIINTGSVTGLTGSKELLDYSMTKGGIHAFTRALSGHLVPKGIRV 189 Query: 178 VATAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLA 237 A APG P P NP S +E E V++ KR + EE +FLA Sbjct: 190 NAVAPG-----PVWTPLNP---SDKE----AEDVEKFGSQTPMKRAAQPEEIAPAYVFLA 237 Query: 238 SDD-ASYITGAVLPVAGG 254 S +SYITG +LP+ GG Sbjct: 238 SPQMSSYITGEILPIVGG 255 >SMc02041 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 254 Score = 102 bits (254), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 123/252 (48%), Gaps = 16/252 (6%) Query: 5 FAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVADLES 64 F+GK VVVTGAA GI R VA A + +V L+DR V DVA G VAD+ Sbjct: 13 FSGKTVVVTGAATGIGRAVAEAFATKRARVALLDRDAAVSDVAVSLGTGH-IAHVADVTD 71 Query: 65 YAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGCHAV 124 G + A+ + +GRIDILIN G + P Y + D + +L A+ Sbjct: 72 EQGVERAVKSVTEAFGRIDILINNAGIG-PLAPAESYPTAEWDRTLAVNLKGAFLMARAI 130 Query: 125 LPQLLQQGRGVIVNVSSNAT-RGIR-RVPYSAAKGGVNALTAALAMEYGELGIRVVATAP 182 P +L+QG G IVN++S A GI V Y A+K G+ +T +A+E+G G+ V A +P Sbjct: 131 APGMLEQGSGRIVNMASQAAIIGIEGHVAYCASKAGIIGMTNCMALEWGPRGVTVNAVSP 190 Query: 183 GGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDDAS 242 V G + W E ++ + +R++ E A +L+LA A+ Sbjct: 191 --------TVVETELGLTG----WAGEKGERARAAIPTRRFAKPWEIAASVLYLAGGAAA 238 Query: 243 YITGAVLPVAGG 254 + GA L + GG Sbjct: 239 MVNGANLMIDGG 250 >AGc3482 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 262 Score = 102 bits (253), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 82/267 (30%), Positives = 132/267 (49%), Gaps = 39/267 (14%) Query: 5 FAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDR---------AEFVHDVAAEAAGGRG 55 +G+V +VTGA GI + +A+G+A+ G V L+DR A+F+ AG + Sbjct: 14 LSGQVALVTGAGSGIGQRIAMGLAQSGANVALLDRRTDDGLAQTADFI-----ARAGRKS 68 Query: 56 KGLVADLESYAGAQAAMDEAVQR----YGRIDILINGVGGAIRMRPYAQYTPTQIDAEIR 111 + AD+ S + A+ +AV R G + + +N G A P + +Q + Sbjct: 69 IQIAADVTS----KQALTDAVARTQAELGALSLAVNAAGIA-NANPAEEMEESQFQTMMD 123 Query: 112 RSLMPTLYGCHAVLPQLLQQGRGVIVNVSSNA----TRGIRRVPYSAAKGGVNALTAALA 167 +L C A +L+ GRG IVN++S + RG+ + Y+A+K GV ++ ++A Sbjct: 124 INLKGVFLSCQAEANAMLKNGRGAIVNIASMSGVIVNRGLMQCHYNASKAGVIHMSKSMA 183 Query: 168 MEYGELGIRVVATAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLE 227 ME+ GIRV +PG T P + PE Q + Q +Q++ G ++ Sbjct: 184 MEWVGRGIRVNTISPGYTATP---MNTRPEMVHQTKLFEEQTPMQRMAG---------VD 231 Query: 228 EQIAPILFLASDDASYITGAVLPVAGG 254 E + P +FL SD AS++TG L V GG Sbjct: 232 EMVGPAIFLLSDAASFVTGVDLLVDGG 258