BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0208.p (251 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1822 [Q] COG3473 Maleate cis-trans isomerase Rso Ralstonia so... 351 9e-97 APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pe... 136 5e-32 PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 131 1e-30 PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus ho... 130 2e-30 PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus a... 126 4e-29 SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus s... 115 6e-26 SMb20431 [Q] COG3473 Maleate cis-trans isomerase Sme Sinorhizobi... 75 1e-13 mlr7137 Non COG 71 3e-12 >RSc1822 [Q] COG3473 Maleate cis-trans isomerase Rso Ralstonia solanacearum Length = 252 Score = 351 bits (900), Expect = 9e-97, Method: Compositional matrix adjust. Identities = 172/248 (69%), Positives = 201/248 (81%) Query: 4 RLYRIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESD 63 R YRIGQIVPSSNTTMETEIPA+L AR+ +PERFTFHSSRMRMK V KEELAAMD +SD Sbjct: 5 RAYRIGQIVPSSNTTMETEIPAILRARELTQPERFTFHSSRMRMKHVTKEELAAMDADSD 64 Query: 64 RCAVELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDA 123 RCA+ELSDARVDVLGYACLVAIM+MG GYHR S+ RL T NG APVVTSAGAL+D Sbjct: 65 RCALELSDARVDVLGYACLVAIMSMGLGYHRVSQARLHQRTQENGGPAPVVTSAGALVDG 124 Query: 124 LKVIGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIV 183 LK IGA+K+ ++APYMKPLT+LV+DYI +EG EV+D +LEIPDNL+V R DP I Sbjct: 125 LKAIGARKVSLLAPYMKPLTQLVIDYIENEGIEVVDSISLEIPDNLEVGRQDPRAPIEIT 184 Query: 184 AQMDTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGA 243 ++ T+ VDAIV SACVQMPSLP + +E TG PV++++VATTY MLKRL L+ VPG Sbjct: 185 KRLKTTGVDAIVASACVQMPSLPSVQAIEDRTGLPVLSSSVATTYLMLKRLGLDTKVPGF 244 Query: 244 GALLSGAY 251 G+LLSGAY Sbjct: 245 GSLLSGAY 252 >APE2520 [Q] COG3473 Maleate cis-trans isomerase Ape Aeropyrum pernix Length = 272 Score = 136 bits (342), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 81/242 (33%), Positives = 135/242 (55%), Gaps = 13/242 (5%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESDRCA 66 RIG IVPSSNTTME E+ M PE + H+ R+ ++KV EEL M+ S A Sbjct: 42 RIGLIVPSSNTTMEPEMWMM-------APEGVSIHTGRVPLEKVSVEELLKMEEYSAIEA 94 Query: 67 VELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDALKV 126 +L+ A VD++ Y C + G GY + +L++ + VVT+A A+++ALK Sbjct: 95 EKLATAGVDLIVYGCTTGSLIGGPGYDLKIADKLSSASGVQ-----VVTTATAVVEALKA 149 Query: 127 IGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVAQM 186 +GA +I V PY++ + + +++RH G++++D+++L + NLD+ R P R+ + + Sbjct: 150 LGASRIAVATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSL 209 Query: 187 DTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGAGAL 246 DAI +S C + ++ VI +E TG PV+++ A+ + LK L + AG + Sbjct: 210 SIEGADAIFIS-CTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTLGIREAGFKAGTI 268 Query: 247 LS 248 S Sbjct: 269 FS 270 >PH0985 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 238 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 75/241 (31%), Positives = 128/241 (53%), Gaps = 13/241 (5%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESDRCA 66 RIG IVPSSNTTME E M PE + H++RMR+++V +E L M+ + A Sbjct: 7 RIGLIVPSSNTTMEPEFWKM-------APEGVSIHTARMRLREVTEESLLEMERHAKDAA 59 Query: 67 VELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDALKV 126 +EL+DA VDV+ Y C + G+GY ++ A + +T++ A+++AL Sbjct: 60 LELADAEVDVIVYGCTSGSLIKGKGYDKE-----IAKNLEEASGIKTITTSTAVLNALNT 114 Query: 127 IGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVAQM 186 + +K+VV PY+ + E +++ G EVL + L I N ++ R +P + ++ Sbjct: 115 LDIQKVVVATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKNTEIGRQEPWVAYKLALEV 174 Query: 187 DTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGAGAL 246 T + D + +S C ++ +I ++E G PV+T+ AT + LK ++++ G L Sbjct: 175 YTPEADGLFIS-CTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMKVKEKYDMYGTL 233 Query: 247 L 247 L Sbjct: 234 L 234 >PH1150 [Q] COG3473 Maleate cis-trans isomerase Pho Pyrococcus horikoshii Length = 236 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 74/241 (30%), Positives = 131/241 (54%), Gaps = 14/241 (5%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESDRCA 66 RIG IVPSSNTTME E+ + L PE + H+SRM +K + EEL M+ + A Sbjct: 7 RIGLIVPSSNTTMEMELHSFL-------PEGVSLHTSRMPLKSITDEELFKMNSYAIEAA 59 Query: 67 VELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDALKV 126 L+DA V+V+ + C G+ + ++ E ++ + T++ A+++AL V Sbjct: 60 SLLADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEE-----VNIETFTTSTAVLEALDV 114 Query: 127 IGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVAQM 186 + A+ +++V PY + + +++ G+EVLD + L + DNL++ + +P + + M Sbjct: 115 LDAQSLLIVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAM 174 Query: 187 DTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGAGAL 246 DAI +S C + + +I ++E G PVIT+ AT + L+++++ +P G L Sbjct: 175 FAGDADAIFIS-CTNLRTFEIIEKLERDLGVPVITSNQATLWMALRQIDVRDQLP-LGKL 232 Query: 247 L 247 L Sbjct: 233 L 233 >PAB2420 [Q] COG3473 Maleate cis-trans isomerase Pab Pyrococcus abyssi Length = 236 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 73/241 (30%), Positives = 129/241 (53%), Gaps = 14/241 (5%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESDRCA 66 RIG IVPSSNTTME E ++L PE + H+SRM +K V +EEL M + A Sbjct: 7 RIGLIVPSSNTTMEMEFHSVL-------PEGVSLHTSRMPLKSVTEEELLNMSSYAIEAA 59 Query: 67 VELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDALKV 126 L+DA V+V+ + C G+ + ++ E ++ + T++ A+I+AL + Sbjct: 60 KLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKE-----VNVETFTTSTAVIEALNI 114 Query: 127 IGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVAQM 186 + + + V+ PY+ + + +++ G+EV+D R L I DN+++ + +P + Sbjct: 115 LEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKAS 174 Query: 187 DTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGAGAL 246 T VD I +S C + +I ++E G PV+T+ AT + L++++++ +P G L Sbjct: 175 FTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGKL 232 Query: 247 L 247 L Sbjct: 233 L 233 >SSO2939 [Q] COG3473 Maleate cis-trans isomerase Sso Sulfolobus solfataricus Length = 242 Score = 115 bits (289), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 66/244 (27%), Positives = 128/244 (52%), Gaps = 12/244 (4%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRMKKVVKEELAAMDGESDRCA 66 ++G I+PSSNTT+E E + I+ R T H SR++++ V E+LA M+ E++R A Sbjct: 5 KVGVIIPSSNTTVEYEFSNVF---NQIKEYRITPHFSRIKLRNVTIEDLAEMERETERAA 61 Query: 67 VELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDALKV 126 ELS ++ YAC + G +H + QR+ A P ++G++I+AL+ Sbjct: 62 SELSTILPQIISYACTTGSLFKGPKHHEEIIQRI-----EKIAKVPATATSGSVINALRR 116 Query: 127 IGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAI---V 183 + K++V++ PY++ + + +++ G+E++ + I +N+ + + P + V Sbjct: 117 LDIKRLVLLTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNV 176 Query: 184 AQMDTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGA 243 + D + +S C + + +++++E T KPVI++ AT + L ++ L V Sbjct: 177 LRNVKGDYDGVFIS-CTNLRTFEILSRLEKETKKPVISSNSATLWETLYKINLNIKVREL 235 Query: 244 GALL 247 G L Sbjct: 236 GHLF 239 >SMb20431 [Q] COG3473 Maleate cis-trans isomerase Sme Sinorhizobium meliloti Length = 261 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 60/228 (26%), Positives = 111/228 (48%), Gaps = 16/228 (7%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRM-RMKKVVKEELAAMDGE-SDR 64 RIG ++ +++ T E + M+ + +R + +R+ V E L AM ++ Sbjct: 24 RIGLVILATDHTTEVDFQRMVAS------DRIGVYVTRIPYANPVTPENLRAMQPSLTEA 77 Query: 65 CAVELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDAL 124 A+ L D +DV+ Y+C A + +G ++ + A A +A VVT A + L Sbjct: 78 AALILPDETLDVVMYSCTSASVVIG-------DREVAAAIKAAKPEAAVVTPTAASVQGL 130 Query: 125 KVIGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVA 184 + + A +I V+ PY + + DY G+ + + L + D+ ++AR P + A Sbjct: 131 RALSANRISVLTPYTIETSRPMADYFAERGFAIDRFTCLGLTDDREMARISPKEIVAFAK 190 Query: 185 QMDTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLK 232 + DA+ +S C + + VIA++E GKPV+++ AT +A L+ Sbjct: 191 EAAAPASDALFIS-CTAVRAAGVIAEIEQAIGKPVVSSNYATAWACLR 237 >mlr7137 Non COG Length = 259 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 62/244 (25%), Positives = 108/244 (44%), Gaps = 16/244 (6%) Query: 7 RIGQIVPSSNTTMETEIPAMLTARQAIRPERFTFHSSRMRM-KKVVKEELAAMDGE-SDR 64 R+G I+ +++ T E + R+ + ER H +R+ E L M + Sbjct: 24 RVGLIILATDHTSEPDF------RRMVASERIGVHVARIPYANPTTPENLRKMQPALTAG 77 Query: 65 CAVELSDARVDVLGYACLVAIMAMGRGYHRQSEQRLTAHTAANGADAPVVTSAGALIDAL 124 A+ L D ++D + Y+C A + +G +E A G PVVT A + L Sbjct: 78 AALILPDEKLDAICYSCTSASVVIG-----DAEIETAIQAAKPGV--PVVTPPMAGVRGL 130 Query: 125 KVIGAKKIVVVAPYMKPLTELVVDYIRHEGYEVLDYRALEIPDNLDVARHDPARLPAIVA 184 G ++I ++ PY + + Y G+E+ + L D+ ++AR PA L + Sbjct: 131 NAFGVRRISILTPYTVETSRPMAAYFAARGFEIASFTCLGFEDDREMARIPPAALVDLAR 190 Query: 185 QMDTSQVDAIVLSACVQMPSLPVIAQVEAMTGKPVITAAVATTYAMLKRLELERIVPGAG 244 Q+ Q DA+ +S C + + +E G+PV+T+ AT + L+ E P G Sbjct: 191 QVTDPQADALFVS-CTALRGALAVTGMEQAIGRPVVTSNQATAWNCLRLCGDETPRPEFG 249 Query: 245 ALLS 248 L++ Sbjct: 250 QLMT 253