BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0205.p (207 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGpA348 [Q] COG1335 Amidases related to nicotinamidase Atu Agrob... 79 5e-15 SMc02412 [Q] COG1335 Amidases related to nicotinamidase Sme Sino... 76 5e-14 Ta0454 [Q] COG1335 Amidases related to nicotinamidase Tac Thermo... 70 2e-12 MK0504 [Q] COG1335 Amidases related to nicotinamidase Mka Methan... 70 3e-12 mll6720 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesor... 70 4e-12 VC0771_1 [Q] COG1535 Isochorismate hydrolase Vch Vibrio cholerae 68 1e-11 mlr7064 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesor... 67 4e-11 VNG1100C [Q] COG1335 Amidases related to nicotinamidase Hbs Halo... 65 1e-10 SMa1471 [Q] COG1335 Amidases related to nicotinamidase Sme Sinor... 65 2e-10 SMa1724 [Q] COG1335 Amidases related to nicotinamidase Sme Sinor... 64 2e-10 >AGpA348 [Q] COG1335 Amidases related to nicotinamidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 239 Score = 79.3 bits (194), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 59/201 (29%), Positives = 87/201 (43%), Gaps = 19/201 (9%) Query: 20 KPPFGLLIVDFVNSFAD---PQQFGGGNIPQ--------------AIERTRSLLAHARQQ 62 K LL++D N++ D P G ++ + I T+ LL R Sbjct: 31 KGKVALLVIDMQNTYLDRPDPDALSGNDLVRYHAWAPFHERMHGTVIPNTQHLLERFRAL 90 Query: 63 GWPVAHSRIVFADDDADRNIFGMKVPGM--VTLKEDLPGSAIVPQLAPANGELVVRKTVP 120 G V +RI D + PG + L +D S I+PQ+AP +GE+VV KT Sbjct: 91 GLDVFFARIACLRADGRDRSLSQRKPGWNNLLLPKDEEASQILPQIAPRDGEIVVTKTTD 150 Query: 121 SAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPH 180 SA GT+L L G+ ++ G T CV ++V F +VV D ++ H Sbjct: 151 SALTGTNLRLLLANMGITHVVCVGIFTDQCVSSTVRSLADESFDVIVVDDACAAGSVDLH 210 Query: 181 EANLFDMAQKYAAVMPLDEAM 201 E+ L + YA VM DE + Sbjct: 211 ESELQRLHMIYANVMTTDEVI 231 >SMc02412 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 222 Score = 76.3 bits (186), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 55/190 (28%), Positives = 81/190 (42%), Gaps = 12/190 (6%) Query: 24 GLLIVDFVNSFADP----------QQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVF 73 LL++D N++ DP Q F I T L+ R++ V +RI Sbjct: 29 ALLVIDIQNTYLDPKDTPEETARWQPFYERMRKTVIPNTARLIDECRKRKVEVIFARIAC 88 Query: 74 ADDDADRNIFGMKVPGM--VTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPW 131 D K PG + L ++LP IVP+L P + E+VV KT SA GT+L Sbjct: 89 LKPDGRDRSLSQKKPGFNYLLLPKELPEGQIVPELEPRSDEIVVTKTTDSALTGTNLRLI 148 Query: 132 LTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANLFDMAQKY 191 L G++ ++ G T CV ++V F +VV DC H L + Y Sbjct: 149 LHNMGIKDVICCGIFTDQCVSSTVRSLADESFGVVVVDDCGAAATDDLHRRELEIINMIY 208 Query: 192 AAVMPLDEAM 201 V+ L+E + Sbjct: 209 CHVVSLEEVL 218 >Ta0454 [Q] COG1335 Amidases related to nicotinamidase Tac Thermoplasma acidophilum Length = 182 Score = 70.5 bits (171), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 51/170 (30%), Positives = 85/170 (50%), Gaps = 16/170 (9%) Query: 19 MKPPFGLLIVDFVNSFADPQQFGGGNIPQAIERT---RSLLAHARQQGWPVAHSRIVFAD 75 MKP L++VD VN F G P+A++ R ++ R+ G PV + Sbjct: 1 MKP--ALVVVDMVNEFI----HGRLATPEAMKTVGPARKVIETFRRSGLPVVYVNDSHYP 54 Query: 76 DDADRNIFGMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQH 135 DD + I+G ++K D GS ++ ++ P+ G+ V+ K S F+GT+L L + Sbjct: 55 DDPEIRIWGRH-----SMKGD-DGSEVIDEIRPSAGDYVLEKHAYSGFYGTNLDMILRAN 108 Query: 136 GVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANLF 185 G+ T+++ G CVR + DA+ +R +VV D V R + P+ + F Sbjct: 109 GIDTVVLIGLDADICVRHTAADALYRNYRIIVVEDAVAAR-IDPNWKDYF 157 >MK0504 [Q] COG1335 Amidases related to nicotinamidase Mka Methanopyrus kandleri AV19 Length = 177 Score = 70.5 bits (171), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 56/182 (30%), Positives = 82/182 (45%), Gaps = 13/182 (7%) Query: 24 GLLIVDFVNSFADPQQFGGGNIPQA---IERTRSLLAHARQQGWPVAHSRIVFADDDADR 80 LLIVD + F ++ +P+A + R L R++G V H DD + Sbjct: 3 ALLIVDMIRDFV--EEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEF 60 Query: 81 NIFGMK-VPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQT 139 ++G V G G+ V +L P +G+LVVRK S F+GTSL L V+ Sbjct: 61 KVWGEHAVAGT-------EGAEPVEELKPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKE 113 Query: 140 LLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANLFDMAQKYAAVMPLDE 199 + + G T CV + DA+ G+R VV DCV H L M + A V+ +E Sbjct: 114 IYLTGVCTDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKHMEKLGAEVVDSEE 173 Query: 200 AM 201 + Sbjct: 174 IL 175 >mll6720 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesorhizobium loti Length = 232 Score = 69.7 bits (169), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 50/160 (31%), Positives = 69/160 (43%), Gaps = 4/160 (2%) Query: 49 IERTRSLLAHARQQGWPVAHSRIVFADDDADRNIFGMKVPGMVTL---KEDLPGSAIVPQ 105 I T LLA ARQ G +RI D K+PG L K D P S +V + Sbjct: 71 IPNTARLLALARQNGIECLFARIACHTKDGRDRSLSQKMPGWNNLLLPKNDAP-SQLVAE 129 Query: 106 LAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRP 165 L PA E+VV KT SA GT+L L G++ ++ G T CV ++V + Sbjct: 130 LQPAGDEIVVTKTTDSALTGTNLRLILHNLGIRNVICCGIFTDQCVSSTVRSLADESYAV 189 Query: 166 LVVSDCVGDRALGPHEANLFDMAQKYAAVMPLDEAMQVVG 205 +V+ DC H L + Y VM E +++ Sbjct: 190 IVLEDCCAAATEDLHRKELEIINMIYCHVMSSGELCKIMA 229 >VC0771_1 [Q] COG1535 Isochorismate hydrolase Vch Vibrio cholerae Length = 216 Score = 68.2 bits (165), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 54/185 (29%), Positives = 88/185 (47%), Gaps = 8/185 (4%) Query: 25 LLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVFADDDADRNIFG 84 LLI D F IP I+ + L AHA+Q G PV ++ D A+R + Sbjct: 31 LLIHDMQEYFVHYFDSQAEPIPSLIKHIQQLKAHAKQAGIPVVYTAQPANQDPAERALLS 90 Query: 85 -MKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVA 143 PG L E+ +AI+ LAP +G++ + K SAF + L WL + G L++ Sbjct: 91 DFWGPG---LSEE---TAIIAPLAPESGDVQLTKWRYSAFKKSPLLDWLRETGRDQLIIT 144 Query: 144 GAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANLFDMAQKYAAVMPLDEA-MQ 202 G + ++ +DA + +P V+ D V D +L HE +L ++ + AV +A ++ Sbjct: 145 GVYAHIGILSTALDAFMFDIQPFVIGDGVADFSLSDHEFSLRYISGRTGAVKSTQQACLE 204 Query: 203 VVGQR 207 + Q Sbjct: 205 IAAQH 209 >mlr7064 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesorhizobium loti Length = 233 Score = 66.6 bits (161), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 53/188 (28%), Positives = 81/188 (43%), Gaps = 27/188 (14%) Query: 18 EMKPP-FGLLIVDFVNSFADPQQFGGGNIPQA----------IERTRSLLAHARQQGWPV 66 E++P L+I+D F P GG + IE +S+L+ R +G+ V Sbjct: 24 ELRPDNTALIIIDMQTDFCGP----GGYVDHMGYDLSLVRAPIEPIKSVLSAMRAKGYTV 79 Query: 67 AHSRIVFADDDAD----------RNIFGMKVPGMV--TLKEDLPGSAIVPQLAPANGELV 114 H+R D AD R G+ PG L PG I+P L P GE + Sbjct: 80 IHTREGHRPDLADLPANKRWRSRRINAGIGDPGPCGRILVRGEPGWDIIPDLYPIEGEPI 139 Query: 115 VRKTVPSAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGD 174 + K +F T L L Q G++ +++ G T CV ++ +A G+ +++ DC G Sbjct: 140 IDKPGKGSFCATDLELILNQRGIENIVLTGITTDVCVHTTMREANDRGYECMMLEDCCGA 199 Query: 175 RALGPHEA 182 G H A Sbjct: 200 TDFGNHLA 207 >VNG1100C [Q] COG1335 Amidases related to nicotinamidase Hbs Halobacterium sp. NRC-1 Length = 183 Score = 65.1 bits (157), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 54/156 (34%), Positives = 67/156 (42%), Gaps = 16/156 (10%) Query: 24 GLLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVFADDDADRNIF 83 L++VDF FADP +G N P A LLA R P+AH R N Sbjct: 7 ALVLVDFQQGFADPA-WGDRNNPDAEAHAEELLAAWRDAAAPIAHVR---------HNST 56 Query: 84 GMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVA 143 P L++ PG A LAPA E K+V AF T+L WL +L+V Sbjct: 57 EATSP----LRQGEPGFAYTDGLAPAADEPEFVKSVNGAFVDTALEGWLRDRDTGSLVVC 112 Query: 144 GAVTSGCVRASVVDAMSWGFRPLVVSDCVG--DRAL 177 G T CV +V A + GF +V D DR L Sbjct: 113 GLTTDHCVSTTVRMADNRGFDVTLVRDATATHDRTL 148 >SMa1471 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 226 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 45/137 (32%), Positives = 67/137 (48%), Gaps = 1/137 (0%) Query: 55 LLAHARQQGWPVAHSRIVFADDDADRNIFGMKVPGMVTLKE-DLPGSAIVPQLAPANGEL 113 L + AR+ G PV H + D R + G + D P +AI P++AP E Sbjct: 44 LQSAARRSGIPVFHCAYIVDLADNLRPFHPIGPDGRSAFSDKDDPLTAICPEVAPLPSER 103 Query: 114 VVRKTVPSAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVG 173 ++ K SAF + L L GV+ L+VAG T CV A+V DA++ G L+V D G Sbjct: 104 LLVKNEASAFGKSPLIGELRDAGVEWLVVAGVWTEACVDATVKDAINLGLHVLLVKDGCG 163 Query: 174 DRALGPHEANLFDMAQK 190 L H+ + ++A + Sbjct: 164 SGGLAMHQTGILNLANR 180 >SMa1724 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 260 Score = 64.3 bits (155), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 45/154 (29%), Positives = 68/154 (44%), Gaps = 13/154 (8%) Query: 24 GLLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVFADDDADRNIF 83 +++VD N + +F I +A+E L+ AR+ G D NI Sbjct: 84 AIIVVDLQNDYLTTGKFPLVGIDKALENAARLVDAARRSG-------------DLVVNIR 130 Query: 84 GMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVA 143 G G+ I+P +AP +GE VV K P++F T LA L+ GV + V Sbjct: 131 HESPAGAPFFVSGTEGAEIIPNIAPQHGEAVVTKRYPNSFRETELASLLSSAGVDEVTVI 190 Query: 144 GAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRAL 177 GA++ C+ A+ A G++ VV D R L Sbjct: 191 GAMSHMCIDATARAASDLGYKTTVVEDACATRDL 224