BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0184.p (386 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0219 [I] COG1960 Acyl-CoA dehydrogenases Rso Ralstonia solana... 181 4e-45 MT3178 [I] COG1960 Acyl-CoA dehydrogenases MtC Mycobacterium tub... 166 7e-41 Rv3094c [I] COG1960 Acyl-CoA dehydrogenases Mtu Mycobacterium tu... 166 8e-41 Rv3570c [I] COG1960 Acyl-CoA dehydrogenases Mtu Mycobacterium tu... 119 2e-26 MT3675 [I] COG1960 Acyl-CoA dehydrogenases MtC Mycobacterium tub... 119 2e-26 AGc4598 [I] COG1960 Acyl-CoA dehydrogenases Atu Agrobacterium tu... 99 2e-20 >RSp0219 [I] COG1960 Acyl-CoA dehydrogenases Rso Ralstonia solanacearum Length = 393 Score = 181 bits (458), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 124/379 (32%), Positives = 181/379 (47%), Gaps = 24/379 (6%) Query: 18 LAPLLAEIRARRDEFAAQ-RFVPKDFIAQLKKAGVYRAATPQCFGGSGMSPMGFLQLIER 76 LAP LA + F Q R +P++ +L G+YR TP+ FGG + M F +IER Sbjct: 17 LAPKLAA----KSVFIEQARHLPQEIADELAARGLYRMLTPRSFGGHEVDIMTFASVIER 72 Query: 77 ISVADASVGWVASFGSASVYLAA-LPRETQAKLYAKGPDLTFAGGLFPIQPATQVEG--- 132 ++ ADAS W S + A LP + A+L+A P + AG P AT+ E Sbjct: 73 LARADASAAWCTFISCTSALVGAYLPTDEAARLFAD-PAVKLAGVYAPGGRATRTERNGV 131 Query: 133 -GWMVDGTWKFASGSKGADVLGVGIGAAVPGQAGSKP----------RTAVLPPEQVEII 181 G++V G W + SGS+ A+ + +G KP ++ V EQV I Sbjct: 132 PGFLVSGHWNWGSGSRNANYI---VGGCFIIDDDGKPELTPAGKPNVQSMVFSAEQVTIA 188 Query: 182 DNWDVMGLLGTGSHDLKVSQQFVPEEWTFIRGGEPTIDEPLYRYPTIAYAAQVLAVVNLG 241 D W +GL GTGS++ V FVP T + + PLYR+P +A V LG Sbjct: 189 DTWHSVGLCGTGSNEFSVEGVFVPTSRTASLVVDTPLPRPLYRFPVFGVLGLSIAAVALG 248 Query: 242 VARAAIDEINQMSGGRVAITGAPKLADRSYVRIEVAKAEAKLRSARAFLYDATGEVWHSI 301 +A AI+ + +S + LA+RS ++ +A A+LR+ARAFL++ + W Sbjct: 249 IAHRAIESLIDLSKQKTPQGSTRPLAERSGTQLAIAYCSARLRAARAFLHEVLEDTWAVS 308 Query: 302 LAGNPVTPEQVSLLRLTAVEIAKVGNEVTQAMFGLAGTTAIYSNHPLQRYLRDAAVVKQH 361 +P+ +RL A V M LAG +A+Y P+QR LRD V QH Sbjct: 309 KGSDPIAVSHRRDIRLAATFATTESASVVDRMHRLAGGSAVYQASPIQRCLRDIHVATQH 368 Query: 362 AFLGEGIYDGAGAVTLGVP 380 + E Y+ AG + L +P Sbjct: 369 IMVSESTYELAGRLLLDLP 387 >MT3178 [I] COG1960 Acyl-CoA dehydrogenases MtC Mycobacterium tuberculosis CDC1551 Length = 395 Score = 166 bits (420), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 124/378 (32%), Positives = 181/378 (47%), Gaps = 7/378 (1%) Query: 5 ENPAALAAREEDALAPLLAE-IRARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCFGGS 63 E P + E + LA +A RAR E R +P + + +L++AG+ RA P+ Sbjct: 17 EPPVNQSETEIEILAEKIARWARARSAEIERDRRLPDELVTRLREAGLLRATMPREVAAP 76 Query: 64 GMSPMGFLQLIERISVADASVGWVASFGSASVYLAA-LPRETQAKLYAKGPDLTFAGGLF 122 ++P L+ E ++ DAS GW S S L A LP ++ +++ G AG Sbjct: 77 ELAPGRALRCAEAVARGDASAGWCVSIAITSALLVAYLPARSREEMFGGGR-GVAAGVWA 135 Query: 123 PIQPATQVEGGWMVDGTWKFASGSKGADVLGVGIGAAVPGQAGSKPRTAVLPPEQVEIID 182 P A V+GG +V G W F SG AD++ G P L ++++++D Sbjct: 136 PRGTARSVDGGVVVSGRWPFCSGINHADIMFAGCFV----DDRQVPSVVALNKDELQVLD 191 Query: 183 NWDVMGLLGTGSHDLKVSQQFVPEEWTFIRGGEPTIDEPLYRYPTIAYAAQVLAVVNLGV 242 W +GL GTGSHD FVP + F P +D PLYR+P + A + LG Sbjct: 192 TWHTLGLRGTGSHDCVADDVFVPADRVFSVFDGPIVDRPLYRFPVFGFFALSIGAAALGN 251 Query: 243 ARAAIDEINQMSGGRVAITGAPKLADRSYVRIEVAKAEAKLRSARAFLYDATGEVWHSIL 302 ARAAID++ +++GG+ + LA+RS + A AE+ L +ARA Y+ W Sbjct: 252 ARAAIDDLVELAGGKKGLGSTRTLAERSATQAAAATAESALGAARALFYEVIEAAWQVSH 311 Query: 303 AGNPVTPEQVSLLRLTAVEIAKVGNEVTQAMFGLAGTTAIYSNHPLQRYLRDAAVVKQHA 362 V + LRL A + +V ++M+ LAG TAIY N PLQR RDA H Sbjct: 312 DAEAVPVTMRNRLRLAATHAVRTSADVVRSMYDLAGGTAIYDNAPLQRRFRDAFTATAHF 371 Query: 363 FLGEGIYDGAGAVTLGVP 380 + E + G V L P Sbjct: 372 QVNEASRELPGRVLLDQP 389 >Rv3094c [I] COG1960 Acyl-CoA dehydrogenases Mtu Mycobacterium tuberculosis H37Rv Length = 376 Score = 166 bits (420), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 118/356 (33%), Positives = 172/356 (48%), Gaps = 6/356 (1%) Query: 26 RARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCFGGSGMSPMGFLQLIERISVADASVG 85 RAR E R +P + + +L++AG+ RA P+ ++P L+ E ++ DAS G Sbjct: 20 RARSAEIERDRRLPDELVTRLREAGLLRATMPREVAAPELAPGRALRCAEAVARGDASAG 79 Query: 86 WVASFGSASVYLAA-LPRETQAKLYAKGPDLTFAGGLFPIQPATQVEGGWMVDGTWKFAS 144 W S S L A LP ++ +++ G AG P A V+GG +V G W F S Sbjct: 80 WCVSIAITSALLVAYLPARSREEMFGGGR-GVAAGVWAPRGTARSVDGGVVVSGRWPFCS 138 Query: 145 GSKGADVLGVGIGAAVPGQAGSKPRTAVLPPEQVEIIDNWDVMGLLGTGSHDLKVSQQFV 204 G AD++ G P L ++++++D W +GL GTGSHD FV Sbjct: 139 GINHADIMFAGCFV----DDRQVPSVVALNKDELQVLDTWHTLGLRGTGSHDCVADDVFV 194 Query: 205 PEEWTFIRGGEPTIDEPLYRYPTIAYAAQVLAVVNLGVARAAIDEINQMSGGRVAITGAP 264 P + F P +D PLYR+P + A + LG ARAAID++ +++GG+ + Sbjct: 195 PADRVFSVFDGPIVDRPLYRFPVFGFFALSIGAAALGNARAAIDDLVELAGGKKGLGSTR 254 Query: 265 KLADRSYVRIEVAKAEAKLRSARAFLYDATGEVWHSILAGNPVTPEQVSLLRLTAVEIAK 324 LA+RS + A AE+ L +ARA Y+ W V + LRL A + Sbjct: 255 TLAERSATQAAAATAESALGAARALFYEVIEAAWQVSHDAEAVPVTMRNRLRLAATHAVR 314 Query: 325 VGNEVTQAMFGLAGTTAIYSNHPLQRYLRDAAVVKQHAFLGEGIYDGAGAVTLGVP 380 +V ++M+ LAG TAIY N PLQR RDA H + E + G V L P Sbjct: 315 TSADVVRSMYDLAGGTAIYDNAPLQRRFRDAFTATAHFQVNEASRELPGRVLLDQP 370 >Rv3570c [I] COG1960 Acyl-CoA dehydrogenases Mtu Mycobacterium tuberculosis H37Rv Length = 394 Score = 119 bits (297), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 104/380 (27%), Positives = 164/380 (43%), Gaps = 20/380 (5%) Query: 17 ALAPLLAEIRARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCFGGSGMSPMGFLQLIER 76 A+ LL EIR R R +P + + L G + PQ +GG P F + R Sbjct: 15 AIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGGLQCDPALFFEATRR 74 Query: 77 ISVADASVGWVASF-GSASVYLAALPRETQAKLYAKGPDLTFAGGLFPIQPATQVEGGWM 135 ++ S GWV+S G + +LA + Q +++ + P + P+ V+GG++ Sbjct: 75 LASVCGSTGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSYAPMGAGVVVDGGYL 134 Query: 136 VDGTWKFASGSKGADVLGVG---IGAAVPGQAGSKPRTAVLPPEQVEIIDNWDVMGLLGT 192 V+G+W ++SG A VG I P GS ++P + EI D W V+GL GT Sbjct: 135 VNGSWNWSSGCDHASWTFVGGPVIKDGRPVDFGS----FLIPRSEYEIKDVWYVVGLRGT 190 Query: 193 GSHDLKVSQQFVPEEWTFIR---------GGEPTIDEPLYRYPTIAYAAQVLAVVNLGVA 243 GS+ L V FVP F+ GG T P+Y+ P ++ +G+A Sbjct: 191 GSNTLVVKDVFVPRH-RFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTTISAPIVGMA 249 Query: 244 RAAIDEINQMSGGRV-AITGAPKLADRSYVRIEVAKAEAKLRSARAFLYDATGEVWHSIL 302 A + G RV A K D + ++ +A+A + + +A L + + + Sbjct: 250 YGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLA 309 Query: 303 AGNPVTPEQVSLLRLTAVEIAKVGNEVTQAMFGLAGTTAIYSNHPLQRYLRDAAVVKQHA 362 AG + E + R V +F +G TA+ + P+QR+ RDA + HA Sbjct: 310 AGKEIPFELRARARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWRDAHAGRVHA 369 Query: 363 FLG-EGIYDGAGAVTLGVPP 381 E Y G G+PP Sbjct: 370 ANDPERAYVIFGNHEFGLPP 389 >MT3675 [I] COG1960 Acyl-CoA dehydrogenases MtC Mycobacterium tuberculosis CDC1551 Length = 394 Score = 119 bits (297), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 104/380 (27%), Positives = 164/380 (43%), Gaps = 20/380 (5%) Query: 17 ALAPLLAEIRARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCFGGSGMSPMGFLQLIER 76 A+ LL EIR R R +P + + L G + PQ +GG P F + R Sbjct: 15 AIDNLLPEIRDRAQATEDLRRLPDETVKALDDVGFFTLLQPQQWGGLQCDPALFFEATRR 74 Query: 77 ISVADASVGWVASF-GSASVYLAALPRETQAKLYAKGPDLTFAGGLFPIQPATQVEGGWM 135 ++ S GWV+S G + +LA + Q +++ + P + P+ V+GG++ Sbjct: 75 LASVCGSTGWVSSIVGVHNWHLALFDQRAQEEVWGEDPSTRISSSYAPMGAGVVVDGGYL 134 Query: 136 VDGTWKFASGSKGADVLGVG---IGAAVPGQAGSKPRTAVLPPEQVEIIDNWDVMGLLGT 192 V+G+W ++SG A VG I P GS ++P + EI D W V+GL GT Sbjct: 135 VNGSWNWSSGCDHASWTFVGGPVIKDGRPVDFGS----FLIPRSEYEIKDVWYVVGLRGT 190 Query: 193 GSHDLKVSQQFVPEEWTFIR---------GGEPTIDEPLYRYPTIAYAAQVLAVVNLGVA 243 GS+ L V FVP F+ GG T P+Y+ P ++ +G+A Sbjct: 191 GSNTLVVKDVFVPRH-RFLSYKAMNDHTAGGLATNSAPVYKMPWGTMHPTTISAPIVGMA 249 Query: 244 RAAIDEINQMSGGRV-AITGAPKLADRSYVRIEVAKAEAKLRSARAFLYDATGEVWHSIL 302 A + G RV A K D + ++ +A+A + + +A L + + + Sbjct: 250 YGAYAAHVEHQGKRVRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLA 309 Query: 303 AGNPVTPEQVSLLRLTAVEIAKVGNEVTQAMFGLAGTTAIYSNHPLQRYLRDAAVVKQHA 362 AG + E + R V +F +G TA+ + P+QR+ RDA + HA Sbjct: 310 AGKEIPFELRARARRDQVRATGRSIASIDRLFEASGATALSNEAPIQRFWRDAHAGRVHA 369 Query: 363 FLG-EGIYDGAGAVTLGVPP 381 E Y G G+PP Sbjct: 370 ANDPERAYVIFGNHEFGLPP 389 >AGc4598 [I] COG1960 Acyl-CoA dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 436 Score = 98.6 bits (244), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 82/243 (33%), Positives = 110/243 (45%), Gaps = 23/243 (9%) Query: 1 MSVMENPAALAAREEDALAPLLAEIRARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCF 60 + V NP A +A L AR+ E A R VP I L+ G + P+ F Sbjct: 36 LPVQTNPHVRLASRVAGIADLFRS-SARQTEEA--RRVPASHIEALRGIGYFDIVKPRAF 92 Query: 61 GGSGMSPMGFLQLIE---RISVADASVGWVASFGSASVYLAAL-PRETQAKLYAKGPDLT 116 G G F +L+E +S A AS GWVA SA +L A+ P E QA ++ + PD Sbjct: 93 NGKGGD---FAELVEANIELSAACASTGWVAGLLSAHQWLLAMFPEEAQADVWGENPDAL 149 Query: 117 FAGGLFPIQPATQVEGGWMVDGTWKFASGSKGADVLGVGIGAAVPGQAGSKPRTAVL--P 174 G P++ A +GG+ + G W FASG + A A +P + KP A L P Sbjct: 150 LCGSYAPVKMAEAADGGYRLSGKWAFASGCENAQ--WSLCAAILPPKGEGKPVPAFLLVP 207 Query: 175 PEQVEIIDNWDVMGLLGTGSHDLKVSQQFVPEE--WTF-------IRGGEPTIDEPLYRY 225 Q + D W V+GL GTGS L + FVPE TF GG +E L+ Sbjct: 208 ASQYVVEDTWHVVGLAGTGSKTLVLDDVFVPEHRVLTFPDATSGCTPGGRYYAEESLFNM 267 Query: 226 PTI 228 P + Sbjct: 268 PLL 270