BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0145.p (297 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0065 [K] COG0583 Transcriptional regulator Rso Ralstonia sola... 249 7e-66 PA0159 [K] COG0583 Transcriptional regulator Pae Pseudomonas aer... 216 4e-56 RSc2327 [K] COG0583 Transcriptional regulator Rso Ralstonia sola... 208 1e-53 YPO0799 [K] COG0583 Transcriptional regulator Ype Yersinia pestis 205 9e-53 RSp1146 [K] COG0583 Transcriptional regulator Rso Ralstonia sola... 204 2e-52 PA0876 [K] COG0583 Transcriptional regulator Pae Pseudomonas aer... 201 2e-51 SMc00831 [K] COG0583 Transcriptional regulator Sme Sinorhizobium... 197 2e-50 PA0056 [K] COG0583 Transcriptional regulator Pae Pseudomonas aer... 195 1e-49 mlr5541 [K] COG0583 Transcriptional regulator Mlo Mesorhizobium ... 192 8e-49 BMEII1135 [K] COG0583 Transcriptional regulator Bme Brucella mel... 191 1e-48 >RSc0065 [K] COG0583 Transcriptional regulator Rso Ralstonia solanacearum Length = 312 Score = 249 bits (635), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 124/287 (43%), Positives = 188/287 (65%), Gaps = 1/287 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MRVF+KVVE GSF RAA+ L +SNAV TR++ADLEAHLGTRLLNR+TR LSLT+ G+ YL Sbjct: 7 MRVFSKVVELGSFARAAQHLSMSNAVVTRYVADLEAHLGTRLLNRTTRSLSLTDVGETYL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 +R + IL ++D+AE+L + ++ G+L I FG L +L+ + Q YPEV D++ Sbjct: 67 QRCQQILLDIDEAESLATSRSQALVGSLRIVAPVMFGLHVLPMLLARFQQLYPEVTFDVA 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LS+R ID+VE+G D+G S + ++ R++ A +++ A+PAY+ + P++ ED++ Sbjct: 127 LSDRPIDIVEDGRDVGIMVSELGLGSHLVARRMISAEMVMAATPAYLRQHSTPQRAEDIA 186 Query: 181 RHNCLNFSHEYLRHHWHVNT-NGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSL 239 H + H LRH W + +G + VP+ +V+++ L+ LA MGI + SY Sbjct: 187 DHRVIAMWHPQLRHEWEMTAPDGQSIAVPVQPSLVTSNAELMHRAVLADMGIAILSSYMA 246 Query: 240 GDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISAR 286 DL SGRLV+VLP + + +S+VYPSR+ L KVR+F +++ A+ Sbjct: 247 RPDLESGRLVRVLPQYKLPHRSLSLVYPSRKFLPTKVRTFIDYMLAQ 293 >PA0159 [K] COG0583 Transcriptional regulator Pae Pseudomonas aeruginosa Length = 312 Score = 216 bits (551), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 118/289 (40%), Positives = 169/289 (58%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MRVFA+VV+ GSF AA+ LD+S A +R I+DLEAHL TRLL+R+TRRL+LTEAG+ YL Sbjct: 7 MRVFARVVDSGSFTSAAQALDLSTAQVSRLISDLEAHLQTRLLHRTTRRLALTEAGERYL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 ER R IL +++ AEA + RP G L + G GQ L M++ Y +P+V ++++ Sbjct: 67 ERCRGILEDIEVAEAEAAGAHIRPCGRLRVQSLMGMGQHHLVPMIARYGALFPDVVIELT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LS+R+ D++EEG D+ Q D+ + ++LG +LCASP Y+ + G P +DL Sbjct: 127 LSQRNPDMLEEGQDVLITGERQLPDSEFVAQRLGSIHSVLCASPGYLQQHGVPRSVDDLE 186 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 RH CL W G V + + N ++ A A +GI L PSY Sbjct: 187 RHVCLRLQDPAYPEGWIFADEQGERTVSPQNTFMVNVAEVMAQAAKADLGIALLPSYVAA 246 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPH 289 LR+G L++VLPG M Q + +YPSRR L AK+R++ + + P Sbjct: 247 AALRNGELLRVLPGHAMHQRTIYALYPSRRYLDAKIRTWVDLLQQELPQ 295 >RSc2327 [K] COG0583 Transcriptional regulator Rso Ralstonia solanacearum Length = 299 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 107/287 (37%), Positives = 173/287 (60%), Gaps = 1/287 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 M++F + VE GS AA LD+S+ +A R + LE LG +LLNR+TRR SLT+ G+ + Sbjct: 7 MQIFVRTVELGSMSAAAVELDLSSQLAGRQVRALEGTLGVKLLNRTTRRQSLTDGGRLFY 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 ER + ILAE++ A+A + P G L I FG L L+ + +P++ +++S Sbjct: 67 ERAKNILAELEAAQAQIDESRALPRGRLRISAPITFGSKALAPRLAEFMNQFPDITVELS 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 L+ R++DLVEE D F + D+ ++ R+L R++LCASP Y+ +G P PEDL Sbjct: 127 LTNRTVDLVEEAFDAAFRTG-DLPDSGLMARRLAPHRLMLCASPGYLKMRGHPRTPEDLR 185 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 +H CL F+H LR W G + +PI K +++SG LR A+AG+GI+L+P+ L Sbjct: 186 KHECLVFAHTSLRTQWSFEGPDGSVKIPIKGKFLADSGEALRAAAVAGVGIILQPAELLA 245 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF 287 +D+++G L ++L ++ + ++Y R ++ K+R+F +F+S F Sbjct: 246 EDIQTGLLTRILKRYEPTPRSLHVLYAPDRRMTQKLRAFLDFVSHAF 292 >YPO0799 [K] COG0583 Transcriptional regulator Ype Yersinia pestis Length = 302 Score = 205 bits (522), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 113/296 (38%), Positives = 177/296 (59%), Gaps = 6/296 (2%) Query: 3 VFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYLER 62 VF +V++GS + AAE L++S A+ATR++A +E G RLL+R+TR+LSLT AG+A LER Sbjct: 9 VFVAIVDRGSMIAAAESLEMSRAMATRYLAQMEQWAGARLLHRTTRKLSLTHAGEATLER 68 Query: 63 VRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDISLS 122 R +L D + E++ G L I C+ GQS L ++ Y + YP+VA+D+ ++ Sbjct: 69 CRRMLEFAQDMDLAEGQESEELRGLLRISCSQSLGQSALAIAVADYLRRYPQVAVDLQMN 128 Query: 123 ERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLSRH 182 R+I+LVEE ID+ + + D ++I R L ++CASPAY++ G PE P+DL+ H Sbjct: 129 NRAINLVEERIDLALRIT-NELDPNLIARPLSTCYSVVCASPAYLSVSGIPESPQDLTVH 187 Query: 183 NCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLGDD 242 NCL +S+ + WH + +G V + + +N V+L AL G GI L+P YS+ Sbjct: 188 NCLTYSY-FGESLWHFDQSGVKSSVAVSGNLSANESVVLLSGALEGAGITLQPIYSVAPY 246 Query: 243 LRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF-PHPENDPWLS 297 + G L+++LP + + + VY SRR + +R+ +F+ F HP+ WLS Sbjct: 247 IARGELIKLLPDYQPQAMGIYGVYASRRQMPTTLRTMLDFLVEWFATHPQ---WLS 299 >RSp1146 [K] COG0583 Transcriptional regulator Rso Ralstonia solanacearum Length = 298 Score = 204 bits (519), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 117/294 (39%), Positives = 173/294 (58%), Gaps = 2/294 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 M VF K VE GSF AAE+L +S+ + + + +E LG RLLNR+TRR SLT+ G+A+ Sbjct: 7 MAVFVKAVELGSFSAAAEVLQMSSQLVGKHVQGIEQRLGVRLLNRTTRRQSLTDFGRAFY 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 ER ++ILAEV+ AE+L P G L I FG L + L Y + YPEV ++++ Sbjct: 67 ERSKIILAEVEIAESLAEETRAVPTGKLRINAPVSFGMHTLAQRLPEYMKRYPEVEVEMT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LS R++DL+ EG D F + D+ +I R L R++LCA+PAY+ + P DL Sbjct: 127 LSNRTVDLINEGYD-AVFRVGELSDSGLIARPLAPYRLVLCAAPAYLEGRPPLTTPWDLR 185 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 +HNCL F++ LR +W + G + VP+ S+++ + G L ALAGMG++L+P + Sbjct: 186 QHNCLGFAYTELRTNWTFDGPDGRVVVPVSSRLMVDHGEPLLCAALAGMGVLLQPLELVR 245 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPHPENDP 294 L G LV VLP ++ M ++Y R ++ K+RSF +F F PE+ P Sbjct: 246 SALADGSLVGVLPQYEPLTRPMHVLYAPDRRVTPKLRSFLDFAVLAF-GPESAP 298 >PA0876 [K] COG0583 Transcriptional regulator Pae Pseudomonas aeruginosa Length = 314 Score = 201 bits (510), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 113/290 (38%), Positives = 170/290 (58%), Gaps = 2/290 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MR F V E GSF AA+ LD++ A +R +A LEAHL TRLL+R+TRR++LTEAGQ YL Sbjct: 7 MRAFVSVAETGSFTAAAQRLDLTTAYVSRAVAKLEAHLQTRLLHRTTRRIALTEAGQRYL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 R + ILA +++AEA RP+GTL ++ G GQ L + +S Y + YPEV D++ Sbjct: 67 MRAQQILAYIEEAEAEAGDAHARPSGTLKVHAMTGIGQHYLIKAISQYCETYPEVGFDLT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 L+ R D+++EG DI + + D+ + + +G ILCASP YIA G P+ P +LS Sbjct: 127 LANRITDILDEGYDISVVIAPELPDSGFVSKCIGQTYSILCASPGYIAAHGFPKLPAELS 186 Query: 181 RHNCLNFSHEYLR-HHWHVN-TNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYS 238 H CL + + W + +G L + N+ + + ++GMGI P Y+ Sbjct: 187 AHRCLRLVNSVMSLDRWLFDGPDGQELACINQTHFQVNTADAMTEAVVSGMGIGALPVYA 246 Query: 239 LGDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFP 288 + L+ G LV+VLP + + + + +YPSR+ L AK+R++ EF+ P Sbjct: 247 AVNGLKDGSLVRVLPRYSLFHLNVYALYPSRQYLDAKIRTWVEFLREHMP 296 >SMc00831 [K] COG0583 Transcriptional regulator Sme Sinorhizobium meliloti Length = 298 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 113/292 (38%), Positives = 175/292 (59%), Gaps = 13/292 (4%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 + +FA+VV GS A L S AV ++ I LE LG RLL R+TR++SLTEAGQ + Sbjct: 7 LEIFARVVSTGSMSAAGRALGFSAAVISKRIKRLEDRLGARLLQRTTRQISLTEAGQGFY 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 +RV ILA +++AEA S + + GTL I FG+ + L+ + + +P++ L I Sbjct: 67 DRVLGILAGLEEAEAYASGRSSQVQGTLRISAPTSFGRMHIAPHLTGFMKDHPDLKLHIV 126 Query: 121 LSERSIDLVEEGID----IGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQP 176 L++ D+V EG D IG S D+S++ R+L R +LCA+P+Y+AR GAP + Sbjct: 127 LTDDFTDIVAEGFDLAIRIGELS-----DSSLVARKLAPVRRLLCAAPSYVARHGAPTEI 181 Query: 177 EDLSRHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPS 236 +L++H CL + +W + G L +V+NS ++R+ +AG GI LR + Sbjct: 182 GELAKHICLPAHNN---DNWKLEGPDGSLSYRPEGPLVTNSSEIIREAVIAGAGIALRST 238 Query: 237 YSLGDDLRSGRLVQVLPGFDMG-QVIMSMVYPSRRLLSAKVRSFAEFISARF 287 + +G +LR GRLVQVLP ++ ++ +S VYPSR+ L AKVR F ++++A + Sbjct: 239 WDIGRELREGRLVQVLPQWEGSHKLTLSAVYPSRQFLPAKVRQFIDYLAALY 290 >PA0056 [K] COG0583 Transcriptional regulator Pae Pseudomonas aeruginosa Length = 306 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 113/291 (38%), Positives = 171/291 (58%), Gaps = 5/291 (1%) Query: 2 RVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYLE 61 RVF +V++ GS AAE LD+S A+ +R++A+LE +G RLL+RSTRRLSLT+AG L Sbjct: 8 RVFVEVIDSGSQTAAAERLDMSRAMVSRYLAELEEWVGARLLHRSTRRLSLTDAGAELLP 67 Query: 62 RVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDISL 121 + R +LA D +A P GTL I + F Q+ L +A+ + YP A+D+ + Sbjct: 68 QCREMLAVADAMQATGQTRRDSPRGTLRITSSLSFAQAWLTRAAAAFVERYPGTAIDLQV 127 Query: 122 SERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLSR 181 + + ++LVEE ID+ + Q D ++I R+LG R ++CA+P Y+ R G P +PEDL+ Sbjct: 128 NSQVVNLVEERIDLALRIANQ-LDPNLIARRLGECRSVICAAPDYLRRHGTPRRPEDLAL 186 Query: 182 HNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLGD 241 HNCL +S+ + R W +G VP+ + +N +L + A AG GI +P YS Sbjct: 187 HNCLTYSY-FGRSLWQFERDGEPTSVPVGGSLSANESTVLLEAAAAGAGISQQPLYSAAP 245 Query: 242 DLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF---PH 289 +RSGRLV +LP + + + VY SRR + +R+ +F+ R PH Sbjct: 246 LIRSGRLVALLPEWSPQVLGIHAVYASRRQMPPALRALLDFLVERMAADPH 296 >mlr5541 [K] COG0583 Transcriptional regulator Mlo Mesorhizobium loti Length = 314 Score = 192 bits (488), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 110/287 (38%), Positives = 165/287 (57%), Gaps = 2/287 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 M+VF V+ GS AA + +S A+ + +A LE LGTRLLNR+TRR SLT+ G +Y+ Sbjct: 7 MQVFVATVQAGSMSSAAAQVGLSPAMVGQHVAALEERLGTRLLNRTTRRQSLTDFGASYI 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 E+ R IL V A+ G L + FG L L Y + PEV LDI+ Sbjct: 67 EQCRDILDRVAIADREAEALQTGARGQLRVTAPTTFGAEVLVPALRRYRELAPEVTLDIT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 L++R++DLVEEG DI F D +I R L R+++CA+P Y+ R+G PE P +LS Sbjct: 127 LTDRNVDLVEEGFDIAFRIGTLP-DNHLIARSLAPYRMMICAAPDYLVRKGMPEHPSELS 185 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 H + F+ R W ++ G V++V V NSG +R A AG+G+V++P+ L Sbjct: 186 SHEVVGFT-PAARSPWRMSKGGDVVEVEPAWTVTVNSGQAVRTAARAGLGVVMQPAILLS 244 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF 287 D+++G+LVQ+ P + + + MS++Y R ++ ++RSF F +A F Sbjct: 245 PDVQAGQLVQLFPDWHLRERPMSLLYHRDRRMTPRLRSFISFAAAEF 291 >BMEII1135 [K] COG0583 Transcriptional regulator Bme Brucella melitensis Length = 297 Score = 191 bits (486), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 104/293 (35%), Positives = 167/293 (56%), Gaps = 4/293 (1%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MR F VVE F AA + S A+ ++++ +LE LG RLLNR+TR+ SLTEAG Y Sbjct: 7 MRAFIDVVEAEGFSAAARKIGRSKALLSKYVRELEDELGARLLNRTTRQFSLTEAGHTYY 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 R I+ E+D + VS + G + + F + +G+ L + A+PE+ LD+ Sbjct: 67 HRALEIIREIDALQDAVSETSSDARGRIKLSAPRTFADAVIGQSLIDFCLAHPEIVLDVR 126 Query: 121 LSERSIDLVEEGIDIGF-FSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDL 179 L +R +DLVEEG D+ + +Q D+S+I ++L +LC +P IAR G P++PEDL Sbjct: 127 LDDRFVDLVEEGFDLAIRITRLQ--DSSLIAKRLSPFHSVLCGAPELIARVGKPQRPEDL 184 Query: 180 SRHNCLNFSHEYLRHHWHV-NTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYS 238 + C+ ++ R++WH N +G ++ VP+ + NS V ++ ALAG+G + P++ Sbjct: 185 TDRPCIVDTNARARNNWHFRNMDGSMMTVPVSGPIEINSPVATKNAALAGLGFCMLPAFI 244 Query: 239 LGDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPHPE 291 D++ G+L+ L F + + VYP RR L AK+R+ +F++ F E Sbjct: 245 AEDEIEQGKLITCLDEFIVRDGGIYAVYPHRRYLPAKIRALVDFMTQWFKERE 297