BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0046.p (314 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0239 [GER] COG0697 Permeases of the drug/metabolite transporte... 186 6e-47 mlr5160 [GER] COG0697 Permeases of the drug/metabolite transport... 139 8e-33 RSp0133 [GER] COG0697 Permeases of the drug/metabolite transport... 133 6e-31 AGl1685 [GER] COG0697 Permeases of the drug/metabolite transport... 120 3e-27 mlr7703 [GER] COG0697 Permeases of the drug/metabolite transport... 108 2e-23 BMEII0677 [GER] COG0697 Permeases of the drug/metabolite transpo... 107 4e-23 AGc2870 [GER] COG0697 Permeases of the drug/metabolite transport... 107 5e-23 RP076 [GER] COG0697 Permeases of the drug/metabolite transporter... 105 1e-22 SMc02817 [GER] COG0697 Permeases of the drug/metabolite transpor... 105 1e-22 RC0106 [GER] COG0697 Permeases of the drug/metabolite transporte... 105 2e-22 >PA0239 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Pae Pseudomonas aeruginosa Length = 292 Score = 186 bits (472), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 111/294 (37%), Positives = 170/294 (57%), Gaps = 7/294 (2%) Query: 3 SAVLSQLPIARTHKLGILFLISGLWTLSLLDTAGKLLALAGYHVVMIAWMRYTINLFFMA 62 +A S LP + GIL + + ++ + D K L+ Y +VM+ W RY ++ M Sbjct: 2 NASTSPLPASDQTLRGILLICAAVFLFASHDGLSKFLS-GFYPIVMVVWARYLVHTLLMV 60 Query: 63 ATLAPLYRRRHGRSILQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLI 122 A P R+G ++++S R +QL R L+G++L F + L+ +PLAE TA+NF APL+ Sbjct: 61 AVFVP----RNGLAVIRSRRPLMQLARALCLIGTSLFFTTGLRYLPLAEATAVNFLAPLL 116 Query: 123 VLAISPWLLGERSYLSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILN 182 V A+S LL E+ L +W+AVL GF G+L ++RP G + L SAL L +L Sbjct: 117 VTALSVPLLKEKVSLGQWLAVLTGFIGVLFIVRPGGALFTPAALFPLGSALCFGLYQLLT 176 Query: 183 RKASQADNPMVTLFYGALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQN 242 R + +D+P + F + +++ +LLVPFFW TP+++ ++++ G GH L Sbjct: 177 RMLANSDSPTTSNFLAGIFNTLIMSLLVPFFW--QTPSLLHGLMMIALGGCGMGGHLLLT 234 Query: 243 SAYRHAEASVLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAY 296 A+RHA + L PF Y QII A +G LVFG PD +LGI +ICASG+ +A+ Sbjct: 235 QAFRHAPPATLAPFSYGQIIFAGLLGLLVFGHVPDAWALLGILVICASGLVVAW 288 >mlr5160 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Mlo Mesorhizobium loti Length = 313 Score = 139 bits (350), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 95/290 (32%), Positives = 154/290 (53%), Gaps = 11/290 (3%) Query: 14 THKLGILFLISGLWTLSLLDTAGKLLALAGYHVVMIAWMRYTINLFFMAATLAPLYRRRH 73 T + IL +++ + LDT+ K L LAG +++AW+R+ +++ + L R Sbjct: 12 TPPVAILCMLTTYVFFTFLDTSSKYLVLAGVSALIVAWVRFAVHVVLVGTLL------RG 65 Query: 74 GRSILQSNRLGL--QLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLL 131 R L+ + L + RG L GST+ L+ + LAE T++ F P+++ A++ LL Sbjct: 66 WRDPLRFRPVNLPAHVLRGVFLFGSTMCNILALRSLQLAETTSIYFFGPMVITALAGPLL 125 Query: 132 GERSYLSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQADNP 191 GE + RW+A+L F G+LI+ RP + G L S L+ I+ R+ S + Sbjct: 126 GEWAGWRRWLAILAAFAGVLIITRPGVGVFGIGHLFALGSMLSNCFYVIMTRRMSSTETS 185 Query: 192 MVTLFYGALVGSVVSTLLVPF-FWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEA 250 + + AL +V+ ++PF F H IL+ G+ +GH+L AYR A Sbjct: 186 ESLILFSALAPTVLLLPMLPFSFSLPHDGW--HWFILLMLGVFGGVGHWLLVQAYRLATT 243 Query: 251 SVLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHK 300 + L P+ Y+Q++ GW+VF QFPDR T++G AII ASG+ I + EH+ Sbjct: 244 TALAPYPYSQMVWMVISGWIVFKQFPDRWTLVGAAIIVASGLYIVHREHR 293 >RSp0133 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Rso Ralstonia solanacearum Length = 316 Score = 133 bits (334), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 105/288 (36%), Positives = 152/288 (52%), Gaps = 10/288 (3%) Query: 18 GILFLISGLWTLSLLDTAGKLLALAGYHVVMIAWMRYTINLFFMAATLAPLYRRRHGRSI 77 GI FLI+ + ++ DT K +++ VVM W RY + ATL P GRS+ Sbjct: 29 GIAFLIAAVAFFAVFDTLTKYISIT-VPVVMALWFRYLFQMVATGATLLP----SRGRSL 83 Query: 78 LQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLGERSYL 137 L++ R LQ+ RG LLL +L F L+ +P+ + TA+ PL+V ++ LGE+ Sbjct: 84 LRTRRPWLQVVRGLLLLLVSLFSFLSLRRMPVGDVTAIVMLTPLVVTFVAAVSLGEQVSA 143 Query: 138 SRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQADNPMVTLFY 197 +RWI + GF G L+VIRP G +G+ L L + A ++ + + D T FY Sbjct: 144 TRWIFMAGGFFGALMVIRPGGAAFDWGMLLPLGMVVANAAFQVVTSRLANVDEAGTTQFY 203 Query: 198 GALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEASVLTPFF 257 VG+++ T ++PF W + L IL+ GI S+ GH L AY A ++LTP+ Sbjct: 204 TGAVGALLLTCMLPFGWQALGGKWWWLLILI--GIVSSSGHALLIMAYTRAPVAMLTPYL 261 Query: 258 YAQIISACGMGWLVFGQFPDRITVLGIAIIC---ASGIGIAYIEHKRA 302 Y QI A GWLVF PD +V GIAII A+G +A E + A Sbjct: 262 YMQIGFAMIGGWLVFSHVPDGWSVAGIAIIAMCGAAGTWVAARERRMA 309 >AGl1685 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 294 Score = 120 bits (302), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 93/290 (32%), Positives = 147/290 (50%), Gaps = 17/290 (5%) Query: 16 KLGILFLISGLWTLSLLDTAGKLLALAGYHVVMIAWMRY---TINLFFMAATLAPLYRRR 72 K G L+ + + D K L A Y V +A +RY + + MAA R Sbjct: 5 KTGFLYALVAFTIFAAQDGISKHLGSA-YPPVFVAMLRYWAFAVFVLLMAA------RSA 57 Query: 73 HG-RSILQSNRLGLQLGRGALLLGSTLIFFSVLK--IVPLAEGTAMNFCAPLIVLAISPW 129 G R + +NR LQ+ RG LL + I FS+ +V LA ++ APLIV A+S Sbjct: 58 GGIRGAVMANRPWLQISRGVLL--AVQIVFSIFSFAVVGLAHSHSILASAPLIVAALSMP 115 Query: 130 LLGERSYLSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQAD 189 LLGE RW A+ VGF G+L++++P GD + + ++A AL S+L R S++D Sbjct: 116 LLGEHVGWRRWCAIFVGFIGVLVILKPDGDGFDNKLIITFVAAFMLALYSVLTRLGSKSD 175 Query: 190 NPMVTLFYGALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAE 249 + M + FY + G+ TL+ PF+W P + +++ IT GH+ A+ A+ Sbjct: 176 SAMTSFFYTGVAGAAALTLVGPFYWVPLAPQ--DWGWMLALCITGMSGHYCLIKAFELAD 233 Query: 250 ASVLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEH 299 A+ + PF Y Q++ +G ++G+ V G AI+ A+G+ + EH Sbjct: 234 AASVQPFSYYQLVLVSIIGVTIYGEVVTSNMVTGAAIVIAAGLFTIWREH 283 >mlr7703 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Mlo Mesorhizobium loti Length = 347 Score = 108 bits (269), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 77/225 (34%), Positives = 115/225 (51%), Gaps = 12/225 (5%) Query: 86 QLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLGERSYLSRWIAVLV 145 + RG + +G+ + F L +PL E +N+ PL+V+ S LGE + RW AV V Sbjct: 109 HVARGVVGVGAMGLGFFALTRLPLPEAITLNYAQPLLVVVFSSVFLGESIRVYRWSAVAV 168 Query: 146 GFTGMLIVIRPS----------GDIPPYGVALGLISALTQALLSILNRKASQADNPMVTL 195 G G+LI+ P D GV L++A A+ +L R Q + + Sbjct: 169 GLIGVLIISWPELTLLNSDEGLDDQEVLGVMAALVAAAISAVAMLLVRNLVQTEKTATIV 228 Query: 196 FYGALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEASVLTP 255 + + SV+S L +PF W S TP V+ A+LV G +G L SAYRHAEASV+ P Sbjct: 229 LWFSSTASVLSLLTLPFGWQSLTP--VQAALLVMAGFCGGLGQILMTSAYRHAEASVVAP 286 Query: 256 FFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHK 300 F Y +I +G+ +FG T++G AI+ A+G+ I + E + Sbjct: 287 FEYTSMILGVVVGYFIFGDVTSLNTLIGGAIVVAAGVFIIWRERQ 331 >BMEII0677 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Bme Brucella melitensis Length = 309 Score = 107 bits (267), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 101/301 (33%), Positives = 152/301 (50%), Gaps = 19/301 (6%) Query: 13 RTHKLGILFLISGLWTLSLLDTAGKLLALAGYHVVMIA-WMRYTIN---LFFMAATLAPL 68 H G+ F++ + L L+D GK LA+ A +MR+ + +FF+ + L Sbjct: 10 EAHVKGMGFMVLSVLMLPLMDAIGKWLAMMDDLPPATATFMRFFVQCWLMFFIILVVGGL 69 Query: 69 YRRRHGRSILQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISP 128 R++ S+ +G L RGAL+ L FF+ +K +PLA+ A+ F PLI+ S Sbjct: 70 ------RALATSHLMG-NLLRGALMGFGGLCFFTAVKYMPLADALAVFFAEPLILTLFSA 122 Query: 129 WLLGERSYLSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQA 188 L E+ R+ AV +G G +IVI+PS +I L L +A+T A+ ILNRK Sbjct: 123 LFLKEKVGWRRFSAVGIGLIGTMIVIQPSFEIFGMVSLLPLATAVTFAIYLILNRKYGAK 182 Query: 189 DNPMVTLFYGALVGSV---VSTLLVPF--FWTSH--TPTMVE-LAILVSTGITSTIGHFL 240 ++P+ Y L G + V+ LL F H P V+ +L+ G T+ H + Sbjct: 183 ESPLTMQLYAGLGGWLFVGVAMLLAADRSFDDMHFALPHSVQPWLLLLLLGTIGTVSHLI 242 Query: 241 QNSAYRHAEASVLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHK 300 A + A AS+L PF Y +I++A MG ++FG FP LGIAII SG + E K Sbjct: 243 VVQASKLAPASMLAPFQYLEIVNAVLMGLIIFGDFPTPSKWLGIAIIIGSGFYVFMRESK 302 Query: 301 R 301 + Sbjct: 303 K 303 >AGc2870 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 311 Score = 107 bits (266), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 92/301 (30%), Positives = 143/301 (47%), Gaps = 13/301 (4%) Query: 18 GILFLISGLWTLSLLDTAGKLLALA-GYHVVMIAWMRYTINLFFMAATLAPLYRRRHGRS 76 G+ + + L ++D K +A++ G + + R+ FF PL + Sbjct: 13 GMTIMAGCMLVLPVMDAIAKYMAVSVGMSPAQVTFYRF----FFQVLCTLPLLLVVSPGA 68 Query: 77 ILQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLGERSY 136 + + R + RG L ++L+FF+ +K +PLA+ A+ F P ++ +S LG+R Sbjct: 69 LFSAKRPWMNCLRGVLHASASLMFFAAVKYMPLADVFAIYFVEPFMLTMMSALFLGDRVG 128 Query: 137 LSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQADNPMVTLF 196 RW A++VGF G LIVI+PS +I + L + A L LNR AD+P+V Sbjct: 129 WKRWTAIVVGFGGALIVIQPSFEIFGWTSLLPVACAFFYTLYLFLNRAIGDADSPLVMQT 188 Query: 197 YGALVGSVV--------STLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHA 248 + G++ L F S + L +LV G S H L A+R A Sbjct: 189 MSGIAGTLFMAAALFAGDALGDKDFAVSLPQSAFALVLLVLLGSLSGYMHLLIVRAFRLA 248 Query: 249 EASVLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHKRAHPLPPA 308 S+L PF Y +IISA +G+ +F FP LGI II ASG+ I + E ++ PP Sbjct: 249 PLSLLAPFQYFEIISATILGYALFSDFPTPSKWLGILIIVASGLFIIWRERQQPASTPPL 308 Query: 309 D 309 + Sbjct: 309 E 309 >RP076 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Rpr Rickettsia prowazekii Length = 294 Score = 105 bits (262), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 66/229 (28%), Positives = 126/229 (55%), Gaps = 4/229 (1%) Query: 73 HGRSILQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLG 132 +G++ L+++R + + RG LL + L I P+ T ++F PL L ++ ++L Sbjct: 60 YGKNALKTSRPFVHVLRGLLLFFGMTSWTYGLTIAPVTTATVVSFAIPLFTLILAVFILN 119 Query: 133 ERSYLSRWIAVLVGFTGMLIVIRP-SGDIPPYGVALGLISALTQALLSILNRKASQADNP 191 E RW+ +VGF G++++++P + D P + L +++A++ A+L I+N+K ++ Sbjct: 120 ENIIWQRWVVTVVGFIGLVVMLKPHTKDFNPEILYL-ILAAISFAMLDIINKKFVVKESM 178 Query: 192 MVTLFYGALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEAS 251 + LFY A+V ++VS + +W TP+ ELA+L G + + F A+ +A+ Sbjct: 179 LSMLFYSAIVTAMVSLPVAMQYWI--TPSSFELALLFVLGSSGSFILFFLLKAFSIVDAT 236 Query: 252 VLTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHK 300 P+ Y +++ + + +F +FPD+ TV G II + + I Y E K Sbjct: 237 ATAPYRYLELVISAIAAYFIFNEFPDKSTVHGAVIIIPATLFIIYSEKK 285 >SMc02817 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Sme Sinorhizobium meliloti Length = 317 Score = 105 bits (262), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 90/289 (31%), Positives = 137/289 (47%), Gaps = 14/289 (4%) Query: 31 LLDTAGKLLALAGYHVVMIAWMRYTINLFFMAATLAPLYRRRHGRSILQSNRLGLQLGRG 90 L+D KL A+A I R+ + + F + P+ R L + L RG Sbjct: 32 LIDIFSKL-AIATVPSAEITAARFLLQIVF----ILPIVLVRGTLFDLTWRKSALHALRG 86 Query: 91 ALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLGERSYLSRWIAVLVGFTGM 150 LL+ + L F + LK++ +A+ A+ F P+I+ + L E R+ A VGF G Sbjct: 87 GLLVVTMLSFITTLKVMEVADAIAIFFVEPIILTILGSIFLKETIGWRRYTACAVGFLGS 146 Query: 151 LIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQADNPMVTLFYGALVGSVVSTLLV 210 L+VI+PS L +++A A+ ++ R +Q ++P F+ + G + L+ Sbjct: 147 LLVIQPSMQEVGLVALLPIVAAFGLAVFLLVTRMVAQNEDPWSMQFHAGIWGGLFCLALL 206 Query: 211 PFFWTSHT-------PTMVELAILVSTGITSTIGHFLQNSAYRHAEASVLTPFFYAQIIS 263 F S + P L+ G+T+TI L AYR A ASVL P Y +I+S Sbjct: 207 WFGEGSGSSVFDPVWPDGRAWYYLLGVGVTATISGVLGVYAYRAAPASVLAPLQYLEIVS 266 Query: 264 ACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEH--KRAHPLPPADP 310 A GWLVFG PD + LGIAII SG+ I + E ++ + P P Sbjct: 267 ATIFGWLVFGDLPDALKWLGIAIIIGSGLYIIWRERQVQKTAGIAPVSP 315 >RC0106 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Rco Rickettsia conorii Length = 294 Score = 105 bits (261), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 64/234 (27%), Positives = 124/234 (52%), Gaps = 5/234 (2%) Query: 73 HGRSILQSNRLGLQLGRGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPLIVLAISPWLLG 132 +G++ L+++R + + RG LL + L I P+ T ++F PL L ++ + L Sbjct: 60 YGKNTLKTSRPFVHILRGLLLFFGMTSWTYGLTIAPVTTATVVSFSIPLFTLILAVFFLN 119 Query: 133 ERSYLSRWIAVLVGFTGMLIVIRPSGDIPPYGVALGLISALTQALLSILNRKASQADNPM 192 E RW+ +VGF G+++ ++P + + +++A++ A+L I+N+K ++ + Sbjct: 120 ENIIWPRWVVTVVGFIGLVVTLKPHAEDFNPEILYFVLAAISFAMLDIINKKFVIKESMI 179 Query: 193 VTLFYGALVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEASV 252 LFY A+V ++VS + +W TP+ ELA+L G + ++ F A+ +A+ Sbjct: 180 SMLFYSAIVTAIVSLPVASQYWL--TPSSFELALLFVLGSSGSLILFFLLKAFSMVDATA 237 Query: 253 LTPFFYAQIISACGMGWLVFGQFPDRITVLGIAIICASGIGIAYIEHK---RAH 303 P+ Y +++ + + +F +FPD+ T+ G II + + I Y E K R H Sbjct: 238 TAPYRYLELVISVIAAYFIFNEFPDKSTLHGAVIIIPTTLFIIYSEKKAMNRKH 291