BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0005.p (564 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1358 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 420 e-117 STM0935 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 409 e-114 poxB [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acet... 402 e-111 ZpoxB [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ace... 401 e-111 ECs0957 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 401 e-111 SSO1012 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 380 e-105 SSO2962 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 366 e-101 Cgl2559 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a... 364 e-100 Ta0816 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 355 2e-97 PA5297 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 351 2e-96 >YPO1358 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Ype Yersinia pestis Length = 573 Score = 420 bits (1079), Expect = e-117, Method: Compositional matrix adjust. Identities = 221/555 (39%), Positives = 335/555 (60%), Gaps = 2/555 (0%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA ++ TL + GV++I+GV GD+LN L+D++ R IEW+G RHEE AA AA + Sbjct: 1 MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+LTG+LAVC G+ GPG HL+ GL++ ++H PVLAI+ +P++ G+ Y QE P+ L Sbjct: 61 AQLTGQLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FR+ + Y +++++P+Q P V A+ QA RGV+ + +P DV + + Sbjct: 121 FRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLPGDVALQPAPEDAAVIWQTPK 180 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 + PP +++ AD +N A ++ + G+GC A DEV+ LA +LQAPV+H RGK+ + Sbjct: 181 LPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI 240 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 +D+P+ +G GLIG + G A+ A+ +++LG+ +PY F PT + +IQID LG Sbjct: 241 EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGA 300 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 PV + + G + + LL +++ K+D AFL +A H LD+ A ++ I Sbjct: 301 HCPVNMALVGDIKTTLTALLPQLDAKSDRAFLDKALEHYRTTRQDLDALA-TANDNQPIH 359 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQ +A+ IS A +DA F D G T+W +++ G +R+L SFN+ ++ ++ QA G Sbjct: 360 PQYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRLLGSFNHGSMANAMPQAIGA 419 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 QA +RQVV GDGGFTML+G+F++ + +LPVK+VVFNN G V +EM+ G + Sbjct: 420 QATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLT- 478 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 +G++ NPDFAA A A G +G V EL L+ A PGP ++DV EL + P Sbjct: 479 DGTDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQ 538 Query: 541 IKMEQIWRFGLAKVR 555 IK EQ F L +R Sbjct: 539 IKFEQAKGFSLYMLR 553 >STM0935 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Sty Salmonella typhimurium LT2 Length = 572 Score = 409 bits (1052), Expect = e-114, Method: Compositional matrix adjust. Identities = 218/556 (39%), Positives = 339/556 (60%), Gaps = 5/556 (0%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA + TL + GV+QI+GV GD+LN L+D++ R IEW+ RHEE AA AA Q Sbjct: 1 MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+LTG LAVC G+ GPG HL+ GL++ ++H PVLAI+ +P++ G+ Y QE P+ L Sbjct: 61 AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FR+ + Y ++++SP+Q P V A+ +A RGV+ + +P DV K + H Sbjct: 121 FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDV-ALKPAPENAVTHWYHA 179 Query: 181 RTPLSPPA-ADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 P+ PA +++ A L+ + ++A+ G+GC GA +E++ALA+KL+AP++H RGK+ Sbjct: 180 PLPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEH 239 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLG 299 V YD+P+ +G GLIG + G + A+ +++LG+ +PY F P+ +K+IQID +G Sbjct: 240 VEYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIG 299 Query: 300 RRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKI 359 + V + + G + ++ LL VE K++ FL +A H LD A++ K I Sbjct: 300 AHSKVDMALVGDIKATLRALLPLVEEKSNRKFLDKALEHYRDARKGLDDLAKLS--DKAI 357 Query: 360 KPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANG 419 PQ +A+ IS A DDA F D G T+W +++ G +R+L SFN+ ++ ++ QA G Sbjct: 358 HPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALG 417 Query: 420 IQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPS 479 QA RQV+ GDGGF+ML+G+F++ V+ +LP+K+VVFNN G V +EM+ G + Sbjct: 418 AQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLT 477 Query: 480 FEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMP 539 +G+E + +FA A ACG G V +++G LQ+ + GP ++DV + +EL + P Sbjct: 478 -DGTELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPP 536 Query: 540 HIKMEQIWRFGLAKVR 555 IK+EQ F L +R Sbjct: 537 QIKLEQAKGFSLYMLR 552 >poxB [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Eco Escherichia coli K12 Length = 572 Score = 402 bits (1033), Expect = e-111, Method: Compositional matrix adjust. Identities = 218/564 (38%), Positives = 340/564 (60%), Gaps = 6/564 (1%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA + TL GV++I+GV GD+LN L+D++ R IEW+ RHEE AA AA + Sbjct: 1 MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+L+G LAVC G+ GPG HL+ GL++ ++H PVLAI+ +P++ G+ Y QE P+ L Sbjct: 61 AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FR+ + Y ++++SP+Q P V A+ +A RGV+ + +P DV K + ++H Sbjct: 121 FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDV-ALKPAPEGATMHWYHA 179 Query: 181 RTP-LSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 P ++P +++ A L+ + ++A+ G+GC GA E++ A K++AP++H RGK+ Sbjct: 180 PQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEH 239 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLG 299 V YD+P+ +G GLIG + G + A+ +++LG+ +PY F PT +K+IQID +G Sbjct: 240 VEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIG 299 Query: 300 RRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKI 359 + V + + G + ++ LL VE K D FL +A LD A K K I Sbjct: 300 AHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLA--KPSEKAI 357 Query: 360 KPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANG 419 PQ +A+ IS A DDA F D G T+W +++ G +R+L SFN+ ++ ++ QA G Sbjct: 358 HPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALG 417 Query: 420 IQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPS 479 QA + ERQVV GDGGF+ML+G+F++ V+ +LPVK+VVFNN G V +EM+ G + Sbjct: 418 AQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIVVFNNSVLGFVAMEMKAGGYLT 477 Query: 480 FEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMP 539 +G+E + +FA A ACG G V E++ LQ+ + GP ++DV + +EL + P Sbjct: 478 -DGTELHDTNFARIAEACGITGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPP 536 Query: 540 HIKMEQIWRFGLAKVRESLIAMKG 563 IK+EQ F L +R ++I+ +G Sbjct: 537 QIKLEQAKGFSLYMLR-AIISGRG 559 >ZpoxB [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] EcZ Escherichia coli O157:H7 EDL933 Length = 572 Score = 401 bits (1030), Expect = e-111, Method: Compositional matrix adjust. Identities = 217/564 (38%), Positives = 339/564 (60%), Gaps = 6/564 (1%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA + TL GV++I+GV GD+LN L+D++ R IEW+ RHEE AA AA + Sbjct: 1 MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+L+G LAVC G+ GPG HL+ GL++ ++H PVLAI+ +P++ G+ Y QE P+ L Sbjct: 61 AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FR+ + Y ++++SP+Q P V A+ +A RGV+ + +P DV K + + H Sbjct: 121 FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDV-ALKPAPEGATTHWYHA 179 Query: 181 RTP-LSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 P ++P +++ A L+ + ++A+ G+GC GA E++ A K++AP++H RGK+ Sbjct: 180 PQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEH 239 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLG 299 V YD+P+ +G GLIG + G + A+ +++LG+ +PY F PT +K+IQID +G Sbjct: 240 VEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIG 299 Query: 300 RRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKI 359 + V + + G + ++ LL VE K D FL +A LD A K K I Sbjct: 300 AHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLA--KPSEKAI 357 Query: 360 KPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANG 419 PQ +A+ IS A DDA F D G T+W +++ G +R+L SFN+ ++ ++ QA G Sbjct: 358 HPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALG 417 Query: 420 IQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPS 479 QA + ERQVV GDGGF+ML+G+F++ V+ +LPVK++VFNN G V +EM+ G + Sbjct: 418 AQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLT 477 Query: 480 FEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMP 539 +G+E + +FA A ACG G V E++ LQ+ + GP ++DV + +EL + P Sbjct: 478 -DGTELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPP 536 Query: 540 HIKMEQIWRFGLAKVRESLIAMKG 563 IK+EQ F L +R ++I+ +G Sbjct: 537 QIKLEQAKGFSLYMLR-AIISGRG 559 >ECs0957 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Ecs Escherichia coli O157:H7 Length = 572 Score = 401 bits (1030), Expect = e-111, Method: Compositional matrix adjust. Identities = 217/564 (38%), Positives = 339/564 (60%), Gaps = 6/564 (1%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVA + TL GV++I+GV GD+LN L+D++ R IEW+ RHEE AA AA + Sbjct: 1 MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 A+L+G LAVC G+ GPG HL+ GL++ ++H PVLAI+ +P++ G+ Y QE P+ L Sbjct: 61 AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FR+ + Y ++++SP+Q P V A+ +A RGV+ + +P DV K + + H Sbjct: 121 FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDV-ALKPAPEGATTHWYHA 179 Query: 181 RTP-LSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 P ++P +++ A L+ + ++A+ G+GC GA E++ A K++AP++H RGK+ Sbjct: 180 PQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEH 239 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLG 299 V YD+P+ +G GLIG + G + A+ +++LG+ +PY F PT +K+IQID +G Sbjct: 240 VEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIG 299 Query: 300 RRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKI 359 + V + + G + ++ LL VE K D FL +A LD A K K I Sbjct: 300 AHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDKALEDYRDARKGLDDLA--KPSEKAI 357 Query: 360 KPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANG 419 PQ +A+ IS A DDA F D G T+W +++ G +R+L SFN+ ++ ++ QA G Sbjct: 358 HPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALG 417 Query: 420 IQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPS 479 QA + ERQVV GDGGF+ML+G+F++ V+ +LPVK++VFNN G V +EM+ G + Sbjct: 418 AQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLT 477 Query: 480 FEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMP 539 +G+E + +FA A ACG G V E++ LQ+ + GP ++DV + +EL + P Sbjct: 478 -DGTELHDTNFARIAEACGITGIRVEKASEIDEALQRAFSIDGPVLVDVVVAKEELAIPP 536 Query: 540 HIKMEQIWRFGLAKVRESLIAMKG 563 IK+EQ F L +R ++I+ +G Sbjct: 537 QIKLEQAKGFSLYMLR-AIISGRG 559 >SSO1012 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Sso Sulfolobus solfataricus Length = 549 Score = 380 bits (977), Expect = e-105, Method: Compositional matrix adjust. Identities = 210/560 (37%), Positives = 331/560 (59%), Gaps = 16/560 (2%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 MP +VA+++I L + G+++I+G+ GD+++PL DAIR+ K ++++ VRHEEGAALAA + Sbjct: 1 MP-SVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSK-VKYVQVRHEEGAALAASVE 58 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 AK+TG+ + C GT+GPG+ HL+ GLY+A+ DHAPV+A++G V + G DY QE + L Sbjct: 59 AKITGKPSACMGTSGPGSIHLLNGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKL 118 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F DVA Y+QI+ +P+ A + +AI +A ++RGVAH+N+P D++ K+S S +T Sbjct: 119 FDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDIL-RKSSEYKGSKNTEVG 177 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 + S D A +LI + + G G RG E+ A K+ AP+++T GK ++ Sbjct: 178 KVKYS---IDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGIL 234 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 P +GG+GL+G P +A+ A++++MLG+ +PY FL +KVIQ+D +G+ Sbjct: 235 PDLDPKVMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGK 294 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRH-HKKI 359 R V L P + L +E K+D + ++ +E W LDS ++ + K + Sbjct: 295 RLDVNLSYP---IPVAEFLNIDIEEKSD-KYYEELKGKKEDW---LDSISKQENSLDKPM 347 Query: 360 KPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSL-GQAN 418 KPQ VA +S DA V DTG VT+W R G Q + S ++G + G Sbjct: 348 KPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIGVPGSVG 407 Query: 419 GIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMP 478 A++ +RQV+ VGDGGFTM + E +T+ ++ LPVK++V+NN + G++ E E G P Sbjct: 408 ASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYP 467 Query: 479 SFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVM 538 + G + NPDF A + G +GF + P+E E ++ L G A+LD +DP+E P+ Sbjct: 468 EW-GVDLYNPDFTKIAESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMP 526 Query: 539 PHIKMEQIWRFGLAKVRESL 558 P + +Q + L+ RE L Sbjct: 527 PKLTFKQAGEYVLSIFREKL 546 >SSO2962 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Sso Sulfolobus solfataricus Length = 552 Score = 366 bits (939), Expect = e-101, Method: Compositional matrix adjust. Identities = 197/558 (35%), Positives = 321/558 (57%), Gaps = 11/558 (1%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M TVADI+++ L + G+++I+G+ GD+++PL DAIRR+K I++I VRHEEGAA AA + Sbjct: 1 MAKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 +KLT + C GT+GPG+ HL+ GLY+A+ DHA V+A++G + + G DY QE + L Sbjct: 61 SKLTSNVTACMGTSGPGSIHLLNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F DVA ++QII +P A + +A+ +A +++GVAHLN+P D++ + + + Sbjct: 121 FDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLPVDILRMEGEEYEGEFYYPK- 179 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 +S D+ A +LI+ + S I G G R V + K+ AP+++ GK ++ Sbjct: 180 ---ISYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGIL 236 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 +GG+GL+G P DAL+ +++++LG+ +PY FLP VIQ+D +G+ Sbjct: 237 SDYDDKVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGK 296 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 R V+L I V + ++ +++ K D +L+ ++ +E+W +L A K +K Sbjct: 297 RIKVSLPIVSDVGEFLLKITPKIKEKDDKFYLKMKDS-KEKWFKELSKAEN--DSSKPMK 353 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQ VA +S + + VVDTG VT+W R G Q+ + S ++G + A G Sbjct: 354 PQRVAFELSKVF-TEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLGSMGIGIPGAVGA 412 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 + E V+ VGDGGF M + E +T+ ++ P+KVV+FNN + G++ E E G P + Sbjct: 413 SFVSDE--VLALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEW 470 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 G E NPDF+ A + G + +V P EL ++++ + P VL+ +DP+E P+ P Sbjct: 471 -GVELYNPDFSRLAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPK 529 Query: 541 IKMEQIWRFGLAKVRESL 558 + Q + L+ RE L Sbjct: 530 LTFTQAKNYILSVFREKL 547 >Cgl2559 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Cgl Corynebacterium glutamicum Length = 579 Score = 364 bits (935), Expect = e-100, Method: Compositional matrix adjust. Identities = 219/561 (39%), Positives = 326/561 (58%), Gaps = 12/561 (2%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M + A+ LIDTL GV++I+G+VGD+LNP+ DA+R+ IEW+ VR+EE AA AA + Sbjct: 1 MAHSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSD-IEWVHVRNEEAAAFAAGAE 59 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 + +TG LAVC + GPG HL+ GLY++ ++ A VLAI+ +P+A G+ + QE PE+L Sbjct: 60 SLITGELAVCAASCGPGNTHLIQGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEIL 119 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F++ + Y +++ +Q + H AI A +GV+ + IP D+ S T+ Sbjct: 120 FKECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPGDIAKEDAGDGTYSNSTISS 179 Query: 181 RTPLS-PPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 TP+ P + A + IN A SV +F G G + A +VL LA K+++P+ H GK Sbjct: 180 GTPVVFPDPTEAAALVEAINNAKSVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQY 239 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLG 299 + +++P +G GL+G DA + A+++++LG+D+PYS+FLP K V Q+D G +G Sbjct: 240 IQHENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLP-KDNVAQVDINGAHIG 298 Query: 300 RRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRH---- 355 RR V +TG VA ++ +L V+ KTD +FL + ER KL S E H Sbjct: 299 RRTTVKYPVTGDVAATIENILPHVKEKTDRSFLDRMLKAHER---KLSSVVETYTHNVEK 355 Query: 356 HKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQR-ILASFNNAAVGTSL 414 H I P+ VA +++LAD DA F VDTG+ +W +I G R + SF + + +L Sbjct: 356 HVPIHPEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTRDFVGSFRHGTMANAL 415 Query: 415 GQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEE 474 A G Q++DR RQV+ GDGG MLLGE +T H+LP+K VVFNN G+V LEM Sbjct: 416 PHAIGAQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLV 475 Query: 475 AGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDE 534 G P F G++ +FA A A G + + P+++ L + LA PGP ++D+ DP+ Sbjct: 476 EGQPEF-GTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNA 534 Query: 535 LPVMPHIKMEQIWRFGLAKVR 555 L + P I EQ+ F A R Sbjct: 535 LSIPPTITWEQVMGFSKAATR 555 >Ta0816 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Tac Thermoplasma acidophilum Length = 548 Score = 355 bits (911), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 197/558 (35%), Positives = 318/558 (56%), Gaps = 11/558 (1%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 M +VA+++ID L ++GV++I+G+ GD++NPL DAIR RI+++ VRHEEGAAL A + Sbjct: 1 MATSVANVIIDVLADLGVKRIYGLPGDSINPLVDAIRTQDRIKFVQVRHEEGAALEAAFE 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 AK TG L VC GT+GPG+ HL+ GLYEA+ PV+A++G + DY QE + + L Sbjct: 61 AKFTGELTVCMGTSGPGSIHLINGLYEAKMSRVPVIALTGQIETDLLYHDYFQEVNLDRL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 F DV+ ++ I +P+ A + +A ++ ++RGV+HL + DV+ P H E Sbjct: 121 FDDVSVFNAQILNPESAAYIVERAYRESVSKRGVSHLTLSVDVLRMGADHR-PVQHMAVE 179 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 + S D AA +IN + +F G G G DEVL A ++ +P++++ K L+ Sbjct: 180 SSHFS---IDPGAAEKIINESKKPLLFVGRGIIGHSDEVLKFAERIGSPIIYSVNAKGLL 236 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 D P +G +GL+G P +A+ A+++++LG+ +PY F+ K++V+Q+D LG+ Sbjct: 237 DDDDPKVMGPLGLLGTKPSVEAMKRADLIVLLGTIFPYVTFMNEKAEVLQVDVDPSNLGK 296 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 R + +VA + Q+ + + K + F + + R+ W + ++ K IK Sbjct: 297 RVRADIAYQCTVADFLSQV--KPDEKPE-KFYSEMSRDRDDWISSMEKLE--SDTSKPIK 351 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 P+ +AR IS AD DA F+VDTG VT+W IR + L S +G + A G+ Sbjct: 352 PEFLARVISKKADRDAVFIVDTGNVTVWATRHIRASKERTFLLSPWLGTMGVGIPGAVGV 411 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 + +RQV+ GDG M + E +T+ ++ PVK+ +FNN + G++ E E G P + Sbjct: 412 -SFATDRQVIAITGDGSAAMTMMELITAKKYSRPVKIAIFNNSKLGMIKFEEEVMGYPEW 470 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 G + NPDF+ A A G +G V P+++E +++ L GPAVLD +D DE P+ P Sbjct: 471 -GVDLLNPDFSKIAEAVGIKGMRVEDPDKVEDAVEEFLRTEGPAVLDAVVDGDERPMPPK 529 Query: 541 IKMEQIWRFGLAKVRESL 558 + Q+ + + +RE L Sbjct: 530 LTFAQVRGYVTSILREKL 547 >PA5297 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Pae Pseudomonas aeruginosa Length = 572 Score = 351 bits (901), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 203/543 (37%), Positives = 316/543 (58%), Gaps = 6/543 (1%) Query: 5 VADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQAKLT 64 VA+I+++TL GVR +G+VGD LN +TDAI R + I+W+ VRHEE AA AA ++ ++ Sbjct: 6 VAEIVVETLEAAGVRHCYGIVGDTLNHVTDAIHRSQ-IQWVHVRHEEAAAFAAGAESYIS 64 Query: 65 GRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRDV 124 GRL C G+ GPG+ H + G+YEA+++ AP++ I+ + G ++ QE + ++ Sbjct: 65 GRLTACAGSCGPGSLHFINGVYEAQRNRAPMVLIASQIVTPQLGMEFPQEVDFKAVYASC 124 Query: 125 AAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVP-SLHTLRERTP 183 + + + + SP+QA V A A +RGVA + +PAD+ A +P S+H + Sbjct: 125 SVFCEQVHSPEQARRVVALACQAALNRRGVAVVILPADISQATVKDDLPFSVHF--PQPV 182 Query: 184 LSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLVGYD 243 L P A++Q A L+ + I+AG+GC+GA D ++ALA +L+AP+ HT R KD V YD Sbjct: 183 LRPSDAELQDVARLLAHGKKIGIYAGSGCQGAHDLLVALADRLKAPIAHTSRAKDFVEYD 242 Query: 244 HPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGRRAP 303 +P +G G++G G + + +L+LG+D+ +++F P K+ +IQ+D G LGRR P Sbjct: 243 NPFNMGMTGMLGIESGFHMMTECDTLLLLGADFAWAQFYPQKATLIQVDRDGSHLGRRHP 302 Query: 304 VTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIKPQS 363 + LG+ G V P ++ LL +E + + +FL + HRER L+ + + + I PQ Sbjct: 303 IDLGVVGDVIPTLEALLPLLEAREERSFLDECLEHRERSLRTLEKEEQAGK-GELIHPQH 361 Query: 364 VARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGIQAL 423 + + A +DA F D G +W I G +R L S + + ++ QA G++ Sbjct: 362 LVSLLDQHASEDALFTADGGSAMVWLLRHIHSNGKRRTLTSLVHGTMANAMPQALGLKKA 421 Query: 424 DRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSFEGS 483 ERQV+ GDGG MLLG+ +T+++ +LP+KVVV NN V LE + G+ + Sbjct: 422 YPERQVISISGDGGLAMLLGDLLTAIQEKLPIKVVVLNNASLNFVELEQKVEGLLD-NYT 480 Query: 484 EFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPHIKM 543 + NPDFA A G G V EELE +Q+ LA PGPA+LDV +P EL + P I+ Sbjct: 481 DLLNPDFARLAEVIGFHGRKVTRSEELEAAVQEFLAQPGPALLDVHTNPAELVMPPKIEF 540 Query: 544 EQI 546 Q+ Sbjct: 541 GQV 543