BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0513.p (210 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4757 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 145 4e-35 STM1270 [E] COG1280 Putative threonine efflux protein Sty Salmon... 141 1e-33 yeaS [E] COG1280 Putative threonine efflux protein Eco Escherich... 138 8e-33 RSc1520 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 135 6e-32 ECs2507 [E] COG1280 Putative threonine efflux protein Ecs Escher... 135 9e-32 ZyeaS [E] COG1280 Putative threonine efflux protein EcZ Escheric... 131 1e-30 RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 100 3e-21 mll2564 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 93 3e-19 AGc2164 [E] COG1280 Putative threonine efflux protein Atu Agroba... 92 1e-18 VCA0355 [E] COG1280 Putative threonine efflux protein Vch Vibrio... 91 1e-18 >PA4757 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 216 Score = 145 bits (367), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 87/212 (41%), Positives = 123/212 (58%), Gaps = 11/212 (5%) Query: 7 SLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAA 66 +LGITD W + + +LLPGP + VL +A+ GV G+ A G+ LGD VLM L+A Sbjct: 3 TLGITDFWTYVLGVVFVILLPGPNSLFVLATSAQRGVATGYRAACGVFLGDAVLMLLSAL 62 Query: 67 GVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAE---------F 117 GVA+LL A P+LF GL+YLGAAYL YLG +L RG P + AA+ F Sbjct: 63 GVASLLKAEPMLFIGLKYLGAAYLFYLGVGML--RGAWRKLRNPEATAAQAEQVDVHQPF 120 Query: 118 RRGFLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNA 177 R+ L++L NPKAI+F+++FF F+DP L+F+ + ++ + LY S L+ G Sbjct: 121 RKALLLSLSNPKAILFFISFFIQFVDPGYAYPGLSFLVLAVILELVSALYLSFLIFTGVR 180 Query: 178 AARRMARNRTIARLASRAAGVFLIGFGIKLTT 209 A R + +A A+ G +GFG+KL T Sbjct: 181 LAAWFRRRQRLAAGATSGVGALFVGFGVKLAT 212 >STM1270 [E] COG1280 Putative threonine efflux protein Sty Salmonella typhimurium LT2 Length = 212 Score = 141 bits (355), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 76/211 (36%), Positives = 121/211 (57%), Gaps = 7/211 (3%) Query: 4 LFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFL 63 +F G+ + W + + +L+PGP T VL + GV+GG+ A G+ +GD +LMFL Sbjct: 1 MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVGRGVKGGYLAACGVFIGDAILMFL 60 Query: 64 AAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGG-----TVVPFSNAAEFR 118 A AGVA L+ P+LF+ ++YLGA YL YLGA++L+A G TVVPF A F+ Sbjct: 61 AYAGVATLIKTTPVLFNIVRYLGAFYLLYLGAKILYATLTSKGRAATETVVPF--GAIFK 118 Query: 119 RGFLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAA 178 R +++L NPKAI+FY++FF F+D ++F + + +F Y S L++ G Sbjct: 119 RALILSLTNPKAILFYVSFFVQFIDVTAPHTGVSFFILATTLEIVSFCYLSFLILSGAFV 178 Query: 179 ARRMARNRTIARLASRAAGVFLIGFGIKLTT 209 + + +A++ + G+ +GF +L T Sbjct: 179 THYIGTKKKLAKVGNSLIGLLFVGFAARLAT 209 >yeaS [E] COG1280 Putative threonine efflux protein Eco Escherichia coli K12 Length = 212 Score = 138 bits (348), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 71/209 (33%), Positives = 120/209 (57%), Gaps = 3/209 (1%) Query: 4 LFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFL 63 +F G+ + W + + +L+PGP T VL + G++GG+ A G+ +GD VLMFL Sbjct: 1 MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFL 60 Query: 64 AAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSN---AAEFRRG 120 A AGVA L+ P+LF+ ++YLGA YL YLG+++L+A +G + A F+R Sbjct: 61 AWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGKNSEAKSDEPQYGAIFKRA 120 Query: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180 +++L NPKAI+FY++FF F+D ++F + A + +F Y S L++ G + Sbjct: 121 LILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILAATLELVSFCYLSFLIISGAFVTQ 180 Query: 181 RMARNRTIARLASRAAGVFLIGFGIKLTT 209 + + +A++ + G+ +GF +L T Sbjct: 181 YIRTKKKLAKVGNSLIGLMFVGFAARLAT 209 >RSc1520 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 220 Score = 135 bits (340), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 82/216 (37%), Positives = 125/216 (57%), Gaps = 10/216 (4%) Query: 4 LFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFL 63 +F GI + W + T+ +LLPGP + VL+ AA+ GVR G+ G+ LGD +LM L Sbjct: 1 MFAHFGIIEFWTFLLGTIFIVLLPGPNSMYVLSVAARRGVRAGYQGACGVFLGDAILMVL 60 Query: 64 AAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLL-------FARGEGGGTVVPFSNAAE 116 +AAGV++LL A+P+LF+ ++ +GAAYLA++G ++L AR GG + E Sbjct: 61 SAAGVSSLLKASPVLFYVVKSIGAAYLAWIGLQMLRGAVRNWRARKAGGSAEQNAARGDE 120 Query: 117 ---FRRGFLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVM 173 F++ +++L NPKAI+F+++FF F+DP AL+F+ +G V +FLY + ++ Sbjct: 121 SHPFKKALVISLLNPKAILFFISFFIQFVDPHFALPALSFVVLGLVCQIGSFLYLTTIIF 180 Query: 174 VGNAAARRMARNRTIARLASRAAGVFLIGFGIKLTT 209 VG A R +A S G IGF KL T Sbjct: 181 VGARLAAAFRARRRLAAGMSSGVGAMFIGFSAKLAT 216 >ECs2507 [E] COG1280 Putative threonine efflux protein Ecs Escherichia coli O157:H7 Length = 212 Score = 135 bits (339), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 69/209 (33%), Positives = 118/209 (56%), Gaps = 3/209 (1%) Query: 4 LFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFL 63 +F G+ + W + + +L+PGP T VL + G++GG+ A G+ +GD VLMFL Sbjct: 1 MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFL 60 Query: 64 AAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSN---AAEFRRG 120 A AG+A L+ P+LF+ ++YLGA YL YLG+++L+A +G A F+R Sbjct: 61 AWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRA 120 Query: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180 +++L NPKAI+FY++FF F+D ++F + + +F Y S L++ G + Sbjct: 121 LILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ 180 Query: 181 RMARNRTIARLASRAAGVFLIGFGIKLTT 209 + + +A++ + G+ +GF +L T Sbjct: 181 YIRTKKKLAKVGNSLIGLMFVGFAARLAT 209 >ZyeaS [E] COG1280 Putative threonine efflux protein EcZ Escherichia coli O157:H7 EDL933 Length = 212 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 68/209 (32%), Positives = 116/209 (55%), Gaps = 3/209 (1%) Query: 4 LFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFL 63 +F G+ + W + + +L PGP T VL + G++GG+ A G+ +GD VLMFL Sbjct: 1 MFAEYGVLNYWTYLVGAIFIVLXPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFL 60 Query: 64 AAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSN---AAEFRRG 120 A AG+A L+ P+LF+ ++YLGA YL YLG+++L+A +G A F+R Sbjct: 61 AWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRA 120 Query: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180 +++L NPKAI+FY++F F+D ++F + + +F Y S L++ G + Sbjct: 121 LILSLTNPKAILFYVSFXVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ 180 Query: 181 RMARNRTIARLASRAAGVFLIGFGIKLTT 209 + + +A++ + G+ +GF +L T Sbjct: 181 YIRTKKKLAKVGNSLIGLMFVGFAARLAT 209 >RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 215 Score = 100 bits (248), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 67/211 (31%), Positives = 105/211 (49%), Gaps = 11/211 (5%) Query: 6 HSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAA 65 +LGI ++ + A ++ + PGP T ++ + G G + G+ G V + A Sbjct: 2 STLGIINLPVFMLAVLLLNVTPGPDTAYIVGRSVSQGRAAGLMSSLGVSAGCCVHVLAVA 61 Query: 66 AGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNA---------AE 116 G+ ALL A+ + F ++ +GAAYL YLG R+L A G P + A A Sbjct: 62 FGLTALLAASAVAFTVIKAIGAAYLIYLGGRMLLA--PAGSDDAPVAQAVATARRPLRAL 119 Query: 117 FRRGFLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGN 176 F +GFL + NPK ++F+++FFP F+DP A F+ +GAV + + S++ V Sbjct: 120 FMQGFLTNVLNPKVVLFFLSFFPQFVDPGASHKAAAFLVLGAVFIVMSTAWNSLVAWVAA 179 Query: 177 AAARRMARNRTIARLASRAAGVFLIGFGIKL 207 + RR+A I R R G IG G +L Sbjct: 180 SVTRRVAGKPGIKRWLDRVVGTAFIGLGARL 210 >mll2564 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 210 Score = 93.2 bits (230), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 71/209 (33%), Positives = 101/209 (48%), Gaps = 28/209 (13%) Query: 18 AATMVFLLLPGPGTFCVLTCAAKGGVRG---GFAAQAGLMLGDVVLMFLAAAGVAALLHA 74 A T +F +PGP +L AA+ RG G A G+ LG V +F AAAG++ L HA Sbjct: 12 ATTAIFAYIPGP---AMLYAAAQTLARGRWSGLTAALGIHLGGYVHVFAAAAGLSVLFHA 68 Query: 75 NPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAEFRRGFL----VTLFNPKA 130 P L+ ++ +GA YL +LG L R E G +P RR F V + NPK Sbjct: 69 VPPLYMAVKLIGALYLIWLGVSLFRKRVE-GDVALPAIERKSARRAFFESITVEVLNPKT 127 Query: 131 IVFYMAFFPLFLD-PATQQGALTFITMGAVIS--------CCTFLYGSVLVMVGNAAARR 181 +F+MAF P F+D A L F+ +G +++ C FL G V+ R Sbjct: 128 AIFFMAFLPQFIDASAALPVWLQFVVLGTIVNLMFSSADIVCVFLAGLVI--------SR 179 Query: 182 MARNRTIARLASRAAGVFLIGFGIKLTTQ 210 + R+ R+ RA G L+G G+ + Q Sbjct: 180 LRRSSRAQRVMQRAGGAVLVGLGVHVALQ 208 >AGc2164 [E] COG1280 Putative threonine efflux protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 210 Score = 91.7 bits (226), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 70/201 (34%), Positives = 106/201 (52%), Gaps = 16/201 (7%) Query: 20 TMVFLLLPGPGTFCVLTCAAKGGVRGGFA---AQAGLMLGDVVLMFLAAAGVAALLHANP 76 T +F +PGP +L AA+ RG FA A G+ +G +F AAAG++ L A P Sbjct: 14 TALFAYIPGPA---MLYAAAQTMARGRFAGLMAVLGIHVGCYFHIFAAAAGLSVLFQAVP 70 Query: 77 LLFHGLQYLGAAYLAYLGARLLFARGEGGG---TVVPFSNAAEFRRGFLVTLFNPKAIVF 133 L+ ++ GA YL +LG +L ++ EG T+ P S F +V + NPK +F Sbjct: 71 WLYLAVKLCGALYLIWLGFSMLRSKLEGEAVNLTIEPKSARRAFIDSIIVDVLNPKTALF 130 Query: 134 YMAFFPLFLDPATQ-QGALTFITMGAVISCCTFLYGS---VLVMVGNAAARRMARNRTIA 189 ++AF P F+DPA L F+ +G ++ F++ S V V++ A RR+ R+ + Sbjct: 131 FLAFLPQFVDPAAAFPVWLQFLILGIAVN---FIFSSADLVGVLLAGAMVRRLKRSSAVQ 187 Query: 190 RLASRAAGVFLIGFGIKLTTQ 210 LA R AG L+G G+ L Q Sbjct: 188 ALARRVAGTVLMGLGVHLAFQ 208 >VCA0355 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 208 Score = 91.3 bits (225), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 60/200 (30%), Positives = 105/200 (52%), Gaps = 15/200 (7%) Query: 17 IAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAAGVAALLHANP 76 I A + ++PGP +++ KG + GF A GL +G V A+ G++A++ ++ Sbjct: 10 IVACLAINMIPGPDVIYIVSNTMKGKLVTGFKAAMGLGVGYFVHTLAASLGLSAIILSSA 69 Query: 77 LLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAE-----FRRGFLVTLFNPKAI 131 + F +++LGAAYL YLG + L + GG T + S + E F +G +V++ NPK Sbjct: 70 VAFSAVKWLGAAYLVYLGVQSLLSMWRGGST-LKVSESVESDKNVFVQGVIVSVLNPKVA 128 Query: 132 VFYMAFFPLFLDPATQQGALTFITMG----AVISCCTFLYGSVLVMVGNAAARRMARNRT 187 +F+++F P F+D ++ ++ + +G +++ C LY S VG+ R R Sbjct: 129 LFFLSFLPQFIDTSSGSASMQLLWLGLLFSVLVTMCNILYAS----VGSWVFSRPNSQRY 184 Query: 188 IARLASRAAGVFLIGFGIKL 207 +R +GV LIG K+ Sbjct: 185 -SRGLEGVSGVLLIGLASKV 203