BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0303.p (278 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the T... 249 3e-66 Cj0556 [R] COG3618 Predicted metal-dependent hydrolase of the TI... 179 4e-45 YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the T... 147 3e-35 AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the T... 65 1e-10 Cgl0451 Non COG 63 9e-10 >RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Rso Ralstonia solanacearum Length = 302 Score = 249 bits (637), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 141/271 (52%), Positives = 178/271 (65%), Gaps = 2/271 (0%) Query: 6 ATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLG 65 A A + IDTHAHVF R LPLA RRYAP+YD T+ Y+A+LD HG+SHGVL+QPSFLG Sbjct: 30 APTARITGIDTHAHVFKRGLPLAGARRYAPSYDATLPAYLAQLDAHGLSHGVLIQPSFLG 89 Query: 66 SDNRYLLAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTG-V 124 DN YLLAA+ + PQRLRG+A++D E L L A G+VGIR NL+G AP P Sbjct: 90 GDNSYLLAALRQAPQRLRGVAVIDPAAPEPFLAQLNAEGIVGIRLNLIG-APDPQLRAPA 148 Query: 125 WRSTLAHIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYL 184 W++ L + LGW VE+H +A LP +LPPLL + VVVDHFGR D AL V+DPGF L Sbjct: 149 WQAALHRLHALGWHVELHTQAHRLPALLPPLLEAQVDVVVDHFGRPDPALSVEDPGFAAL 208 Query: 185 LSTAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQHESLT 244 L+ +VWVK+S +YRNG A +G+ A A P L++ G RL+WGSDWPHTQ+ES Sbjct: 209 LAAGRTRRVWVKVSGAYRNGDAGRGEAIALAAMPRLKDALGTDRLVWGSDWPHTQYESRI 268 Query: 245 DYGHSWQQFTQLVSDEEQRRIITGQTAAQLF 275 Y + L+ D R + +T A+LF Sbjct: 269 GYDSARAFADTLLPDAGDRHQVLVETPARLF 299 >Cj0556 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Cje Campylobacter jejuni Length = 265 Score = 179 bits (455), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 90/273 (32%), Positives = 150/273 (54%), Gaps = 15/273 (5%) Query: 12 LRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71 ++IDTHAH+F + L RY P YD + + Y A LD + + GVLVQPSFLG DN +L Sbjct: 1 MKIDTHAHIFLKKLNTVANARYKPDYDASFKDYKANLDHYDFNKGVLVQPSFLGIDNEFL 60 Query: 72 LAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTG-VWRSTLA 130 L +I + + ++ I +VD ++ +L L+ G+R NL+ G +P+F VW Sbjct: 61 LQSIEK-DENIKAIVVVDENIKFDELKKLKERKACGVRLNLI-GKELPNFKSMVWTQFFE 118 Query: 131 HIAQLGWQVEIHREA-VDLPRVLPPLLAMGLKVVVDHFGRVDAAL-GVDDPGFRYLLSTA 188 ++++L Q+EI R+ +L ++ L+ G +V+DH R +A L ++D L Sbjct: 119 NLSKLKMQIEIQRDLDNNLVDIVENLIPYGCNIVIDHLARANANLTNLED------LICL 172 Query: 189 AGGQVWVKLSASYRN----GGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQHESLT 244 +++ K+S YR EQ +FA++ + +L+EHF ++GSDWPHT E+ Sbjct: 173 KNSRIFFKISGFYRAKIDYANNEQAVKFAKKIYEILKEHFPLSNFVFGSDWPHTNFEANI 232 Query: 245 DYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGI 277 ++ + F ++V ++++ I G A LFG Sbjct: 233 NFSSALVAFNEVVVSKKEQEQILGDNACALFGF 265 >YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Ype Yersinia pestis Length = 289 Score = 147 bits (370), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 89/264 (33%), Positives = 135/264 (51%), Gaps = 5/264 (1%) Query: 14 IDTHAHVFTRD--LPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71 D+HAHV + P+ R Y P E+Y++ L GMS GVLVQ S GSDNRY+ Sbjct: 27 CDSHAHVISPRAIYPMVAERSYTPP-PAPEEKYLSMLAATGMSRGVLVQISVYGSDNRYM 85 Query: 72 LAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTGVWRSTLAH 131 L + R+P+ LRG+A+V DV++Q+L + GV G+R N++ G + F + + + Sbjct: 86 LQVLKRHPEYLRGVAVVTEDVTDQELQDMALAGVRGLRINVLFGGGI-GFDAM-ENLASK 143 Query: 132 IAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYLLSTAAGG 191 IA LGW ++ + LP ++P + + V+DH G + +LG++ PG++ LL Sbjct: 144 IAPLGWHMQFLMDVRQLPALIPRMKKLPCPCVIDHMGHMPVSLGLNHPGYQTLLHMVREY 203 Query: 192 QVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQHESLTDYGHSWQ 251 WVKLS +YR + Q + R++WGSDWPH + D G Sbjct: 204 GWWVKLSGAYRISESWQDGYLDVVPFAQKLIETAPDRMVWGSDWPHVSVSRMPDTGGLRN 263 Query: 252 QFTQLVSDEEQRRIITGQTAAQLF 275 V DE+ R I A+L+ Sbjct: 264 LLAAWVPDEQVRNRILVDNPARLY 287 >AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 292 Score = 65.5 bits (158), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 55/216 (25%), Positives = 90/216 (41%), Gaps = 25/216 (11%) Query: 42 EQYIARLDTHGMSHGVLVQPSFLGSDNRYLLAAIARYPQRLRGIAMVDADVSEQQLLALQ 101 E Y + G+ ++ Q + DN LA +A + + ++DA +E+ + L Sbjct: 51 EDYRRLMQWLGIDRVIITQGNAHQRDNGNTLACVAEMGEAAHAVVIIDATTTEKDMEKLT 110 Query: 102 AGGVVGIR-FNLVGGA-PVPDFTGVWRSTLAHIAQLGWQVEIHREAVDLPRVLPPLLAMG 159 A G VG R +L GGA + + V AH A W V + + L LP L + Sbjct: 111 AAGTVGARIMDLPGGAVNLSELDAV--DERAHAAD--WMVAVQFDGNGLLDHLPRLQKIR 166 Query: 160 LKVVVDHFGRVDAALGVDDPGFRYLLSTAAGGQVWVKLSASYRNGGAEQGQRFARQAWP- 218 + V DH G+ + D P LL G +W K + Y + +R++WP Sbjct: 167 SRWVFDHHGKFFKGIRTDGPEMAALLKLIDRGNLWFKFAGVYES---------SRKSWPY 217 Query: 219 --------LLREHFGAQRLLWGSDWPHTQHESLTDY 246 ++ H +R++WG++WPH Y Sbjct: 218 ADVAAFSRVIAAH-APERIVWGTNWPHNSVRETAAY 252 >Cgl0451 Non COG Length = 250 Score = 62.8 bits (151), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 52/159 (32%), Positives = 74/159 (46%), Gaps = 11/159 (6%) Query: 14 IDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYLLA 73 D+H H+ PL + Y P TVE Y AR++ ++ G +V SF D YL Sbjct: 5 FDSHFHIIDPQHPLIENNGYLPE-PFTVEDYTARVEGLEVAGGAIVSGSFQAFDQGYLKD 63 Query: 74 AIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLV--GGAPVPDFTGVWRST--L 129 A+A G+ + AD S+Q++L L GV +R NL G A + D + R L Sbjct: 64 ALAVLGPGYVGVTQIPADTSDQEILDLDKAGVKAVRLNLKRGGSAGLDDLETLARRVHDL 123 Query: 130 AHIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFG 168 A GW E++ +A +L L LA V +DH G Sbjct: 124 A-----GWHTELYVDARELDE-LESTLASLPAVSIDHLG 156