BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0263.p (173 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc01229 [M] COG1247 Sortase and related acyltransferases Sme Si... 227 8e-60 Z2271 [M] COG1247 Sortase and related acyltransferases EcZ Esche... 224 6e-59 ECs2052 [M] COG1247 Sortase and related acyltransferases Ecs Esc... 224 6e-59 yncA [M] COG1247 Sortase and related acyltransferases Eco Escher... 223 1e-58 PA4866 [M] COG1247 Sortase and related acyltransferases Pae Pseu... 214 6e-56 AGc1654 [M] COG1247 Sortase and related acyltransferases Atu Agr... 212 4e-55 STM1590 [M] COG1247 Sortase and related acyltransferases Sty Sal... 210 1e-54 BMEI0379 [M] COG1247 Sortase and related acyltransferases Bme Br... 184 1e-46 DR1182 [M] COG1247 Sortase and related acyltransferases Dra Dein... 173 2e-43 RSc3133 [M] COG1247 Sortase and related acyltransferases Rso Ral... 144 1e-34 >SMc01229 [M] COG1247 Sortase and related acyltransferases Sme Sinorhizobium meliloti Length = 169 Score = 227 bits (579), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 109/163 (66%), Positives = 132/163 (80%), Gaps = 1/163 (0%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVA-D 59 M+IRDA D+ I IYNDAVANTTAIWNETLVD NR+ WL AR +G+PVL A++ D Sbjct: 1 MEIRDAAETDLPAICAIYNDAVANTTAIWNETLVDVANRKTWLKARNAAGFPVLAALSLD 60 Query: 60 GKVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAG 119 G+V+GYASFG+WRAFDGYRHTVEHSVYV D RG G+GRALM LI RA ALGKHVM+AG Sbjct: 61 GEVVGYASFGEWRAFDGYRHTVEHSVYVRADQRGGGIGRALMLELIDRAEALGKHVMIAG 120 Query: 120 IDASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLML 162 I++ N SI LH++LGF + G++ +VGTKFG+WLDL F+QL+L Sbjct: 121 IESGNLPSIRLHEQLGFRETGHMKEVGTKFGRWLDLTFMQLIL 163 >Z2271 [M] COG1247 Sortase and related acyltransferases EcZ Escherichia coli O157:H7 EDL933 Length = 172 Score = 224 bits (572), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 109/172 (63%), Positives = 124/172 (72%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 M IR A AD A I IYN AV T AIWN+ VD NR W AR +GYPVLV+ DG Sbjct: 1 MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTIAGYPVLVSEEDG 60 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 V GYASFGDWR+FDG+RHTVEHSVYVH DH+G+GLGR L+ LI AR GKHVMVAGI Sbjct: 61 VVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGI 120 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDGRTEPDRIG 172 ++ N S+ LH+ LGF +PQVGTKFG+WLDL F+QL LD RTEPD IG Sbjct: 121 ESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAIG 172 >ECs2052 [M] COG1247 Sortase and related acyltransferases Ecs Escherichia coli O157:H7 Length = 172 Score = 224 bits (572), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 109/172 (63%), Positives = 124/172 (72%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 M IR A AD A I IYN AV T AIWN+ VD NR W AR +GYPVLV+ DG Sbjct: 1 MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTIAGYPVLVSEEDG 60 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 V GYASFGDWR+FDG+RHTVEHSVYVH DH+G+GLGR L+ LI AR GKHVMVAGI Sbjct: 61 VVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGI 120 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDGRTEPDRIG 172 ++ N S+ LH+ LGF +PQVGTKFG+WLDL F+QL LD RTEPD IG Sbjct: 121 ESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAIG 172 >yncA [M] COG1247 Sortase and related acyltransferases Eco Escherichia coli K12 Length = 172 Score = 223 bits (568), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 108/172 (62%), Positives = 124/172 (72%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 M IR A AD A I IYN AV T AIWN+ VD NR W AR +GYPVLV+ +G Sbjct: 1 MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTLAGYPVLVSEENG 60 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 V GYASFGDWR+FDG+RHTVEHSVYVH DH+G+GLGR L+ LI AR GKHVMVAGI Sbjct: 61 VVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGI 120 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDGRTEPDRIG 172 ++ N S+ LH+ LGF +PQVGTKFG+WLDL F+QL LD RTEPD IG Sbjct: 121 ESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAIG 172 >PA4866 [M] COG1247 Sortase and related acyltransferases Pae Pseudomonas aeruginosa Length = 172 Score = 214 bits (545), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 107/168 (63%), Positives = 128/168 (76%), Gaps = 2/168 (1%) Query: 3 IRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAV-ADGK 61 IRDA +AD+ GI IYNDAV NTTAIWNET VD NR+ W R + GYP+LVA A G+ Sbjct: 5 IRDAGVADLPGILAIYNDAVGNTTAIWNETPVDLANRQAWFDTRARQGYPILVASDAAGE 64 Query: 62 VLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGID 121 VLGYAS+GDWR F+G+R TVEHSVYV D RG+GLG L++ALI RARA G HVMVA I+ Sbjct: 65 VLGYASYGDWRPFEGFRGTVEHSVYVRDDQRGKGLGVQLLQALIERARAQGLHVMVAAIE 124 Query: 122 ASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDG-RTEP 168 + N+ SI LH++LGFE G +PQVG KFG+WLDL F+QL LD R+ P Sbjct: 125 SGNAASIGLHRRLGFEISGQMPQVGQKFGRWLDLTFMQLNLDPTRSAP 172 >AGc1654 [M] COG1247 Sortase and related acyltransferases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 165 Score = 212 bits (539), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 99/162 (61%), Positives = 123/162 (75%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 +++RDA + D++GI IYNDAV NTTAIWNE +VD NR+DW AR G+PV+VA+ DG Sbjct: 3 VELRDATVDDLSGIMEIYNDAVVNTTAIWNEVVVDLENRKDWFAARTSRGFPVIVAILDG 62 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 KV GYAS+GDWRAFDGYRHT EHSVYVHKD RG G+G+ LM+ALI A HV++A I Sbjct: 63 KVAGYASYGDWRAFDGYRHTREHSVYVHKDARGHGIGKRLMQALIDHAGGNDVHVLIAAI 122 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLML 162 +A N+ SI LH+ LGF VG +VGTKFG+WLDL ++L L Sbjct: 123 EAENTASIRLHESLGFRVVGRFSEVGTKFGRWLDLTCMELKL 164 >STM1590 [M] COG1247 Sortase and related acyltransferases Sty Salmonella typhimurium LT2 Length = 171 Score = 210 bits (535), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 102/169 (60%), Positives = 120/169 (71%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 M IR A+ AD A IT IYN AV +T AIWN+ VD NR W AR+ GYPVLV+ +G Sbjct: 1 MTIRFADKADCAAITEIYNHAVLHTAAIWNDRTVDTDNRLAWYEARQLLGYPVLVSEENG 60 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 V GYASFGDWR+FDG+R+TVEHSVYVH H+G+GLGR L+ LI AR GKHVMVAGI Sbjct: 61 VVTGYASFGDWRSFDGFRYTVEHSVYVHPAHQGKGLGRKLLSRLIDEARRCGKHVMVAGI 120 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDGRTEPD 169 ++ N+ SI LH LGF +PQVG KFG+WLDL F+QL LD PD Sbjct: 121 ESQNAASIRLHHSLGFTVTAQMPQVGVKFGRWLDLTFMQLQLDEHAAPD 169 >BMEI0379 [M] COG1247 Sortase and related acyltransferases Bme Brucella melitensis Length = 164 Score = 184 bits (466), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 88/162 (54%), Positives = 114/162 (70%) Query: 1 MQIRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG 60 + IR A AD+ + IYNDAV NT AIWNETLVD NR WL R + G+PVLVA +G Sbjct: 3 LLIRHATEADLPALLAIYNDAVENTLAIWNETLVDLENRRQWLENRNRDGFPVLVAEREG 62 Query: 61 KVLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGI 120 +V+GYAS+G +R F+G+RH+ E SVYV + RG G+GR L+ LI AR HV++A I Sbjct: 63 QVVGYASYGPFRPFEGFRHSSELSVYVASNARGGGIGRTLLAELIEEARERKVHVLIASI 122 Query: 121 DASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLML 162 +A N+ SI+LH+ GFE+ G L QVG KFG+WLDL F+Q +L Sbjct: 123 EAGNAASIALHRSQGFEECGTLKQVGQKFGRWLDLLFMQKIL 164 >DR1182 [M] COG1247 Sortase and related acyltransferases Dra Deinococcus radiodurans Length = 174 Score = 173 bits (438), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 87/162 (53%), Positives = 114/162 (70%), Gaps = 1/162 (0%) Query: 3 IRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAVADG-K 61 IR A AD+ GI IYNDAV NTTA ++ ++RE+W AR+++G+PV VA +G Sbjct: 10 IRLATPADLPGILEIYNDAVLNTTASYDLEPATLQSREEWFAARQRAGFPVFVAQGEGGT 69 Query: 62 VLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGID 121 VLG+AS+G +R GY TVEHSVY+ RG GLG ALME LIA ARA HVM+ +D Sbjct: 70 VLGFASYGTFREKPGYNGTVEHSVYIRDGQRGAGLGLALMERLIAEARAQHLHVMLGSVD 129 Query: 122 ASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLD 163 A N+GSI+ H++LGF QV + QVG KFG+WLD+ F++L+LD Sbjct: 130 ADNAGSIAFHERLGFRQVAHFHQVGRKFGRWLDMVFVELLLD 171 >RSc3133 [M] COG1247 Sortase and related acyltransferases Rso Ralstonia solanacearum Length = 182 Score = 144 bits (362), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 74/167 (44%), Positives = 103/167 (61%), Gaps = 1/167 (0%) Query: 3 IRDAELADIAGITPIYNDAVANTTAIWNETLVDDRNREDWLTARRQSGYPVLVAV-ADGK 61 I E + I I N+A+ N+TA+++ + W A+R G+PV+ AV A GK Sbjct: 13 IDCTEAEHASAILEILNEAIVNSTALYDYVPRPPQAMATWFAAKRAGGFPVVGAVDASGK 72 Query: 62 VLGYASFGDWRAFDGYRHTVEHSVYVHKDHRGEGLGRALMEALIARARALGKHVMVAGID 121 +LG+ S+G +RAF Y++TVEHSVYVH + RG LG L+ LI RA+ HV+V ID Sbjct: 73 LLGFGSWGTFRAFPAYKYTVEHSVYVHHECRGRRLGERLLRELIRRAQEAQVHVLVGCID 132 Query: 122 ASNSGSISLHKKLGFEQVGYLPQVGTKFGKWLDLAFLQLMLDGRTEP 168 A+N+ SI LH +LGF G + + G KFG+WLD AF QL L+ +P Sbjct: 133 AANAASIGLHTRLGFVHAGTIREAGFKFGRWLDAAFYQLNLEMPAQP 179