BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0215.p (229 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0242 [Q] COG1335 Amidases related to nicotinamidase Rso Ralst... 390 e-108 PA2698 [Q] COG1335 Amidases related to nicotinamidase Pae Pseudo... 362 e-100 SMa0376 [Q] COG1335 Amidases related to nicotinamidase Sme Sinor... 360 2e-99 AGl1749 [Q] COG1335 Amidases related to nicotinamidase Atu Agrob... 332 5e-91 SMa1821 [Q] COG1335 Amidases related to nicotinamidase Sme Sinor... 188 7e-48 AGl1395GM [Q] COG1335 Amidases related to nicotinamidase Atu Agr... 165 8e-41 RSp0661 [Q] COG1335 Amidases related to nicotinamidase Rso Ralst... 161 1e-39 SMb21405 [Q] COG1335 Amidases related to nicotinamidase Sme Sino... 144 2e-34 SMa2029 [Q] COG1335 Amidases related to nicotinamidase Sme Sinor... 142 6e-34 ECs0982 [Q] COG1335 Amidases related to nicotinamidase Ecs Esche... 120 3e-27 >RSc0242 [Q] COG1335 Amidases related to nicotinamidase Rso Ralstonia solanacearum Length = 228 Score = 390 bits (1002), Expect = e-108, Method: Compositional matrix adjust. Identities = 178/226 (78%), Positives = 199/226 (88%) Query: 3 TNTKLDVLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVE 62 +N KL+VLTP NCQLI IDHQPQMAFGVQS+DRQ LKNN V LAK+A++F+IPT ITTVE Sbjct: 2 SNPKLEVLTPQNCQLIFIDHQPQMAFGVQSIDRQVLKNNTVGLAKAARIFDIPTTITTVE 61 Query: 63 TQGFSGYTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNS 122 T+GFSGYTYPELL VFP ++LERTSMNSWDDQKVRD L NG+KK+VV+GLWTEVCN + Sbjct: 62 TEGFSGYTYPELLDVFPGKELLERTSMNSWDDQKVRDVLARNGRKKVVVSGLWTEVCNTT 121 Query: 123 FALCAMAEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWARKE 182 FALCAMAEG YEIYMVADASGGTSKEAHDYAMQR+IQAG +PVTWQQV+LEWQRDWAR++ Sbjct: 122 FALCAMAEGGYEIYMVADASGGTSKEAHDYAMQRLIQAGAVPVTWQQVLLEWQRDWARRD 181 Query: 183 TYSAVMDIVREHSGAYGMGVDYAYTMVHKADQRPHGNQKTLAPVPA 228 TY AVM +V+EHSGAYGMGVDYAYTMVHKA QR K LA V A Sbjct: 182 TYDAVMQLVKEHSGAYGMGVDYAYTMVHKAPQRTASQHKALAAVAA 227 >PA2698 [Q] COG1335 Amidases related to nicotinamidase Pae Pseudomonas aeruginosa Length = 226 Score = 362 bits (928), Expect = e-100, Method: Compositional matrix adjust. Identities = 171/224 (76%), Positives = 191/224 (85%), Gaps = 3/224 (1%) Query: 7 LDVLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQGF 66 L+VLTP N Q+I ID QPQMAFGVQS+DRQ LKNNVV LAK+AKVF+IP ITTVET F Sbjct: 5 LEVLTPQNSQIIFIDQQPQMAFGVQSIDRQTLKNNVVGLAKAAKVFDIPVTITTVETDSF 64 Query: 67 SGYTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNSFALC 126 SG TYPELL+VFPE ILERTSMNSWDDQ VRD+L A G+KK+VVAGLWTEVCN +FALC Sbjct: 65 SGPTYPELLAVFPEQKILERTSMNSWDDQNVRDSLAAAGRKKVVVAGLWTEVCNTTFALC 124 Query: 127 AMAEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWARKETYSA 186 AM EG YEIYMVADASGGTS +AH YAM RM+QAGV+PVTWQQV+LEWQRDWAR++TY A Sbjct: 125 AMLEGGYEIYMVADASGGTSSDAHKYAMDRMVQAGVVPVTWQQVLLEWQRDWARRDTYDA 184 Query: 187 VMDIVREHSGAYGMGVDYAYTMVHKADQR-PHGNQKTLAPVPAK 229 VM I +EHSGAYGMGVDYAYTMVHKA +R HG + + P PAK Sbjct: 185 VMAIAKEHSGAYGMGVDYAYTMVHKAPERVTHGER--IGPNPAK 226 >SMa0376 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 229 Score = 360 bits (923), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 172/230 (74%), Positives = 197/230 (85%), Gaps = 7/230 (3%) Query: 5 TKLDVLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQ 64 +KL+VLTP N QLI ID QPQMAFGVQS+DRQ LKNNVV LAK+AK+FN+PT ITTVETQ Sbjct: 2 SKLEVLTPANSQLIFIDQQPQMAFGVQSIDRQTLKNNVVGLAKAAKIFNVPTTITTVETQ 61 Query: 65 GFSGYTYPELLSVFPEHDILERTSMNSWDDQKVRDAL---KANGKKKIVVAGLWTEVCNN 121 FSG T+PELL+VFPE+D+LERTSMNSWDDQ VRDAL ANG+KKIVV+GLWTEVCN Sbjct: 62 SFSGNTFPELLAVFPENDLLERTSMNSWDDQNVRDALAKNAANGRKKIVVSGLWTEVCNT 121 Query: 122 SFALCAMAE-GDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWAR 180 +FAL A+ + +YEIYMVADASGGTS +AH YAM RM+QAGVIPVTWQQV+LEWQRDWAR Sbjct: 122 TFALSALHDVPEYEIYMVADASGGTSSDAHKYAMDRMVQAGVIPVTWQQVLLEWQRDWAR 181 Query: 181 KETYSAVMDIVREHSGAYGMGVDYAYTMVHKADQR-PHGNQKTLAPVPAK 229 KETY AV +V+EHSGAYGMG+DYAYTMVH A++R HG K + P PAK Sbjct: 182 KETYDAVTTLVKEHSGAYGMGIDYAYTMVHGAEERVKHG--KRIGPNPAK 229 >AGl1749 [Q] COG1335 Amidases related to nicotinamidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 216 Score = 332 bits (850), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 160/218 (73%), Positives = 182/218 (83%), Gaps = 7/218 (3%) Query: 17 LIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQGFSGYTYPELLS 76 +I ID QPQMAFGVQS+DRQ LKNNVV LAK+AK+FNIPT ITTVET+ FSG T+PELL+ Sbjct: 1 MIFIDQQPQMAFGVQSIDRQTLKNNVVGLAKAAKIFNIPTTITTVETESFSGNTFPELLA 60 Query: 77 VFPEHDILERTSMNSWDDQKVRDALKAN---GKKKIVVAGLWTEVCNNSFALCAMAE-GD 132 VFPE+DILERTSMNSWDDQ VRDAL N G++KIVV+GLWTEVCN +FAL A+ + D Sbjct: 61 VFPENDILERTSMNSWDDQNVRDALAKNAATGRRKIVVSGLWTEVCNTTFALSALHDVAD 120 Query: 133 YEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWARKETYSAVMDIVR 192 YEIYMVADASGGTS +AH YAM RM+QAGVIPVTWQQV+LEWQRDWARKETY AV +V+ Sbjct: 121 YEIYMVADASGGTSLDAHKYAMDRMVQAGVIPVTWQQVLLEWQRDWARKETYDAVTSLVK 180 Query: 193 EHSGAYGMGVDYAYTMVHKADQR-PHGNQKTLAPVPAK 229 EHSGAYGMG+DYA T VH +R HG K + P PAK Sbjct: 181 EHSGAYGMGIDYAVTHVHGGAERVKHG--KRIGPNPAK 216 >SMa1821 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 236 Score = 188 bits (478), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 93/200 (46%), Positives = 131/200 (65%), Gaps = 1/200 (0%) Query: 9 VLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQGFSG 68 +++P + LI+ID Q QM+F +S+D L+NN +A +A F +PT++TTV + FSG Sbjct: 31 LVSPKDHALIMIDFQSQMSFATKSIDAVQLRNNAALVASAAAGFGVPTILTTVAEKSFSG 90 Query: 69 YTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNSFALCAM 128 + E+ FPE +L+RTSMN+W+D V + + GKK++V GLWT VC L A+ Sbjct: 91 PMFAEITDAFPEQPLLDRTSMNTWEDAAVIEEVNRIGKKRLVFCGLWTSVCIVGPTLSAL 150 Query: 129 AEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWARKETYSAVM 188 +G +E Y++ADA G S EAH+ AM RM+QAGV P+T Q MLE QRDWAR +TY Sbjct: 151 DQG-FEAYVIADACGDVSDEAHERAMDRMVQAGVRPMTSLQYMLELQRDWARTDTYDMTT 209 Query: 189 DIVREHSGAYGMGVDYAYTM 208 I ++ GAYG+G+ YA TM Sbjct: 210 GIAKKFGGAYGLGIIYAKTM 229 >AGl1395GM [Q] COG1335 Amidases related to nicotinamidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 214 Score = 165 bits (418), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 85/207 (41%), Positives = 130/207 (62%), Gaps = 5/207 (2%) Query: 1 MTTNTKLD-VLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVIT 59 MT LD +L P + L++IDHQP + S D + NN ALAK AK F++PT++T Sbjct: 1 MTLRNGLDSLLRPEDSVLVLIDHQPYQLANLNSHDPHMVVNNTTALAKLAKAFDVPTILT 60 Query: 60 TVETQGFSGYTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVC 119 +V G + + VFP+ D+++RT +N+W D+ V +A+K +K++++AGLWTEVC Sbjct: 61 SV-IAARGGLLFKHITDVFPDQDVIDRTWVNTWQDENVVNAVKETRRKQLIIAGLWTEVC 119 Query: 120 NNSFALCAMAEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWA 179 + A EG +++ ++ DASGG SKE+H+ A+QRMI+AG +T + EWQRDWA Sbjct: 120 VAMPVIQAAGEG-WDVTVITDASGGISKESHEVAIQRMIRAGANVMTVMALAGEWQRDWA 178 Query: 180 RKETYSAVMDIVREHSGAYGMGVDYAY 206 R E A+ +I+ +H G G G+ Y + Sbjct: 179 RTEHVEALTEILIQHFG--GSGIAYLW 203 >RSp0661 [Q] COG1335 Amidases related to nicotinamidase Rso Ralstonia solanacearum Length = 212 Score = 161 bits (408), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 82/197 (41%), Positives = 121/197 (61%), Gaps = 2/197 (1%) Query: 9 VLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQ-GFS 67 +LTP N +IID+QP + SMDRQ L N +V +++A + +P V +TV + G + Sbjct: 14 LLTPQNAAFVIIDYQPVQVNSIASMDRQLLVNTIVGTSRAAVAYKLPIVHSTVNVKTGLN 73 Query: 68 GYTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNSFALCA 127 P+L V + +RTS+NSW+D + R+A+KA G+KK+++ LWTE C AL A Sbjct: 74 KPPIPQLRKVLGDFPTYDRTSINSWEDVEFREAVKATGRKKLIMTALWTEACLTFPALDA 133 Query: 128 MAEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWARKETYSAV 187 + EG YE+Y+V DA GGTS AH+ A++R+ QAG ++ Q+ E QRDW+R ET A Sbjct: 134 LKEG-YEVYVVVDAVGGTSVAAHEAALRRIEQAGGKMISVAQLFCELQRDWSRSETVPAF 192 Query: 188 MDIVREHSGAYGMGVDY 204 +D+ E G G+ Y Sbjct: 193 IDLFIETGGTAGIQFSY 209 >SMb21405 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 217 Score = 144 bits (362), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 79/172 (45%), Positives = 117/172 (68%), Gaps = 3/172 (1%) Query: 9 VLTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQGFSG 68 +L P + L++IDHQP V S + Q L NN ALAK+AK F +PT++T+V G Sbjct: 21 LLRPQDSVLVLIDHQPYQLTNVNSHEPQMLVNNAAALAKAAKAFAVPTILTSV-IADRGG 79 Query: 69 YTYPELLSVFPEHDILERTSMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNSFALCAM 128 +P++ VFP ++++RT +++W D+KV DA+KA G+K++++AGLW E+C + A+ A+ Sbjct: 80 RIFPQITDVFPGQEVIDRTFIHTWQDEKVVDAVKATGRKQLILAGLWIEIC-VAPAIQAL 138 Query: 129 AEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDWAR 180 EG +++ +V DASGGTS EAH+ A+QRMI AG +TW V EWQR W R Sbjct: 139 GEG-WDVTVVTDASGGTSVEAHEVAIQRMIMAGANMMTWLAVAAEWQRGWPR 189 >SMa2029 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 179 Score = 142 bits (358), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 69/167 (41%), Positives = 110/167 (65%), Gaps = 4/167 (2%) Query: 40 NNVVALAKSAKVFNIPTVITTVETQGFSGYTYPELLSVFPEHDILERTSMNSWDDQKVRD 99 N+ ALAK A+ FN+PT++T+V G + ++ VFP+ ++++RT +N+W D+ V + Sbjct: 4 NHTTALAKLARAFNVPTILTSV-IAARGGLLFKQITDVFPDQEVIDRTWVNTWQDENVVN 62 Query: 100 ALKANGKKKIVVAGLWTEVCNNSFALCAMAEGDYEIYMVADASGGTSKEAHDYAMQRMIQ 159 A+KA G+K++++AGLWTEVC + A EG +++ ++ DASGG SKE+H+ A+QRM Sbjct: 63 AVKATGRKQLIIAGLWTEVCVAMPVIQAAGEG-WDVTVITDASGGISKESHEVAIQRMAA 121 Query: 160 AGVIPVTWQQVMLEWQRDWARKETYSAVMDIVREHSGAYGMGVDYAY 206 AG +T + EWQRDWAR E + +I+ +H G G G+ Y + Sbjct: 122 AGANVMTVMALAGEWQRDWARTEHVEELTEILIQHFG--GSGIAYLW 166 >ECs0982 [Q] COG1335 Amidases related to nicotinamidase Ecs Escherichia coli O157:H7 Length = 208 Score = 120 bits (300), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 65/170 (38%), Positives = 102/170 (60%), Gaps = 2/170 (1%) Query: 10 LTPTNCQLIIIDHQPQMAFGVQSMDRQALKNNVVALAKSAKVFNIPTVITTVETQGFSGY 69 L + ++++DHQ + V+ ++ KNNV+AL AK FN+PT++TT G +G Sbjct: 8 LDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALGDLAKYFNLPTILTTSFETGPNGP 67 Query: 70 TYPELLSVFPEHDILERT-SMNSWDDQKVRDALKANGKKKIVVAGLWTEVCNNSFALCAM 128 PEL + FP+ + R ++N+WD++ A+KA GKK++++AG+ TEVC AL A+ Sbjct: 68 LVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEVCVAFPALSAI 127 Query: 129 AEGDYEIYMVADASGGTSKEAHDYAMQRMIQAGVIPVTWQQVMLEWQRDW 178 EG +++++V DASG ++ A RM QAG +TW V E RDW Sbjct: 128 EEG-FDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRDW 176