BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0189.p (308 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 148 2e-35 mll5767 Non COG 98 2e-20 mlr1708 Non COG 83 1e-15 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 74 5e-13 BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the ... 70 8e-12 mlr7156 Non COG 67 7e-11 SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the ... 65 2e-10 PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TI... 63 7e-10 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 148 bits (373), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 94/294 (31%), Positives = 145/294 (49%), Gaps = 18/294 (6%) Query: 14 PAGRCKTPLRIDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQGLHASITVQ 73 PA R + L IDAHQHFW + W + + +D LP PLL A G+ ++ VQ Sbjct: 8 PAERPEAML-IDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQ 66 Query: 74 ARQGRDETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQMERWGRHKLL-GLRHQLQEHDD 132 + +T F+LE+A I G++GW D+ + ++ ++ R +H G+R LQ +D Sbjct: 67 TMETAADTDFMLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMED 126 Query: 133 ANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALKE 192 + A L+ V L + D L +LP L F ++H + VV++H KP + Sbjct: 127 V-AWIDDAALNAAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLI-- 183 Query: 193 FRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQCLDTALDLF 252 + WR+ + L A P+V CK+SGLLTE R R + +T L+LF Sbjct: 184 ---ATGHYRDWRRNMAALAARPNVHCKLSGLLTERGEQRPEAVRPYA------ETILELF 234 Query: 253 GPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSARIYGL 306 G R++FGSDWPV LA + + R+ + D A++G ++ R Y L Sbjct: 235 GGDRVIFGSDWPVLRLAGDFQGWLDFCRDIVPAK----DHAAVFGGNAIRFYSL 284 >mll5767 Non COG Length = 297 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 83/304 (27%), Positives = 129/304 (42%), Gaps = 34/304 (11%) Query: 24 IDAHQHFWRYRSEDYPWIGAGMDLLAQD-----RLPYQFQPLLE-------AQGLHASIT 71 ID H H W YPW+ G+ A R Y+ LE + +H + Sbjct: 7 IDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLDVG 66 Query: 72 VQARQGRDETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQMERWGRH-KLLGLRHQLQEH 130 ET +L E+A + GI+G+ DLS PD+ E ++R + G+R + H Sbjct: 67 FDPSDPVGETRWLQEIANKRGFPHGIVGYADLSKPDVGELLDRHMEYPNFRGIRQSMNYH 126 Query: 131 DDANTLLTPAP-------LDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLN 183 D P +G R L +R + D+ + + + Q ++LN Sbjct: 127 ADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQIILN 186 Query: 184 HLGKPALKEFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQ 243 H G G F W++ + L P+VACKISG L DW + I Sbjct: 187 HTGMQV-----DGPKHFNAWKKGMHRLAQAPNVACKISG-LGMGDWSWTTAS-----IRP 235 Query: 244 CLDTALDLFGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQD-ALWGESSAR 302 ++ A+ FG R MF +++PV L +S+ VV +E TR P ++ AL+ +++AR Sbjct: 236 YVEEAIAAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE--ITRYYPHEERLALFHDNAAR 293 Query: 303 IYGL 306 Y L Sbjct: 294 HYRL 297 >mlr1708 Non COG Length = 295 Score = 82.8 bits (203), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 74/302 (24%), Positives = 133/302 (44%), Gaps = 35/302 (11%) Query: 24 IDAHQHFWRYRSEDYPWIGAGM--------DLLAQDRLPYQFQPLLEAQGLHASITVQAR 75 IDAH H WR +D PW+ M + + +D +F + G+ ++ VQ Sbjct: 5 IDAHFHIWR--QKDQPWLVGPMVPRIFGPYEPIRRDYPIGEFLQDQKGSGVEKAVYVQTN 62 Query: 76 QGRD----ETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQMERWGRHKLL-GLRHQLQEH 130 ++ E AFL + A I+G+ D++ D+ Q++R +++LL G+R QL H Sbjct: 63 WAKEDFEKEVAFLQQTADETGWPHAIVGYADMTVDDVRPQIDRLMKYRLLRGVRMQLHWH 122 Query: 131 D--------DANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVL 182 + A+ ++ P + V L++ + D+ + + + +L Sbjct: 123 ETPAFRFAASADQVIDPK-VRANVARLKDYGLSFDLQLFPAQMKDGVTLVGENPETNFIL 181 Query: 183 NHLGKPALKEFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIV 242 H G E E W+ LR L+A P+ K+SGL T + D I Sbjct: 182 THAGMLTGME----PEVTETWKAGLRTLSAAPNFYAKLSGLGT------FVHRNDPALIA 231 Query: 243 QCLDTALDLFGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSAR 302 +D A+D+ G LMFGS++P+ L S++ ++ R+ A ++ D W ++ + Sbjct: 232 YIVDNAIDILGADHLMFGSNFPIEKLWTSHAELIKAHRDAVARHGAAAEADIFW-NTAEK 290 Query: 303 IY 304 +Y Sbjct: 291 VY 292 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 297 Score = 73.6 bits (179), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 76/297 (25%), Positives = 129/297 (43%), Gaps = 32/297 (10%) Query: 24 IDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQGLHASITVQARQGRDETAF 83 ID HQH Y W G LA + L+E G+ +I ++ G D+ + Sbjct: 21 IDTHQHLILRGKLGYAWT-RGEPSLAGSFTRADYAALVEGSGVIGTIFMET--GVDDGDY 77 Query: 84 LLEMARRDKRIAGIIGWEDLSA--------PDLAEQMERW---GRH-KLLGLRHQLQEHD 131 E + +AG++G L P+ + + W RH K++G R LQ Sbjct: 78 RAEA----RLVAGMVGEGTLPMFGQIASCRPETDQGFDAWLEECRHLKVVGFRRILQVMP 133 Query: 132 DANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALK 191 D + + VR + + + +D+ H R L + +L+H G P + Sbjct: 134 DDTS--RAENYRRNVRKIGKAGWPVDLCFHSRQLAIAAELVRACPDVRFMLDHCGTPDIA 191 Query: 192 EFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQCLDTALDL 251 F W + + +L ++P++ K+SG+ A G T E + ++T L L Sbjct: 192 G-----NDFPAWSERMADLASLPNLFVKLSGV--TAYCAPGQVT--LESVRPYVETVLKL 242 Query: 252 FGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSARIYGLLT 308 FGP R+++G DWPV L + + + RE+ +T L P +Q ++ G +AR + L T Sbjct: 243 FGPARMVWGGDWPVVDLGSGLPEWIAMTREFLST-LSPDEQASI-GHGNARRFYLAT 297 >BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Bme Brucella melitensis Length = 282 Score = 69.7 bits (169), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 68/257 (26%), Positives = 107/257 (41%), Gaps = 24/257 (9%) Query: 24 IDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQG----LHASITVQARQGRD 79 ID H H + YPW+ + L +D L ++ E G LH + V + Sbjct: 3 IDTHLHLIDKNALSYPWLESA-PALNRDFLFDSYRLQAERAGIGGALHIEVDVAPHAMQA 61 Query: 80 ETAFLLEMARRDKR-IAGIIGWEDLSAPDLAEQMERWGRHKLL-GLRHQLQEHDDANTLL 137 ET + ++ARR I G I P A +ER + GLR L H + + Sbjct: 62 ETDHIRQIARRSGGFIKGAIASCRPEEPGFAAYLERQLSDPFVKGLRRVL--HVVPDEVS 119 Query: 138 TPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALKEFRKGR 197 A + ++ L+ T D+ + + +++H G P +K Sbjct: 120 EGALFRENIKRLEGTGLTFDLCVQPHQIGKAIALADLAPDLCFIVDHCGAPDIKA----- 174 Query: 198 AAFERWRQELRELTAMPHVACKISGLLTEAD---WLRGLCTRDFEHIVQCLDTALDLFGP 254 A + WR+ + E++ P+V KISGL+ D W E ++Q FG Sbjct: 175 GALDGWREGMAEISRRPNVIAKISGLVVYCDAESWTVETLRPYAEQVIQS-------FGW 227 Query: 255 QRLMFGSDWPVCLLAAS 271 R+++GSDWPVC LA+S Sbjct: 228 DRVIWGSDWPVCTLASS 244 >mlr7156 Non COG Length = 297 Score = 66.6 bits (161), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 69/263 (26%), Positives = 103/263 (39%), Gaps = 24/263 (9%) Query: 20 TPLRIDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQG----LHASITVQAR 75 P+ ID H H + YPW+ AG+ L +D + + G LH + V Sbjct: 17 APVIIDTHLHLIDRSALRYPWL-AGVPQLNRDFSYEDYATEAQRVGVERVLHMEVDVDPA 75 Query: 76 QGRDETAFLLEMARR-DKRIAGIIGWEDLSAPDLAEQMERWGRHKLL-GLRHQLQEHDDA 133 ETA + ++R+ + G I D +ER + + G R L H Sbjct: 76 DIEVETARVEGLSRQAGSMLVGAIASCRPEDADFPAYLERQRTNPFVKGFRRVL--HVVP 133 Query: 134 NTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALKEF 193 + L A ++ L T D++ +P+ VL+H G P +K Sbjct: 134 DDLSEGAVFRDNIKRLGGIGLTFDLVVLPHQIPKAIALADLAPDVQFVLDHCGVPDIKG- 192 Query: 194 RKGRAAFERWRQELRELTAMPHVACKISGLLTEAD---WLRGLCTRDFEHIVQCLDTALD 250 A WR + E+ P+V KISG++ AD W G EH + Sbjct: 193 ----GAEHPWRAHMSEIARRPNVMAKISGVVAYADPVNWTVGTLRPYVEHTIAA------ 242 Query: 251 LFGPQRLMFGSDWPVCLLAASYS 273 FG R+++GSDWPVC L S Sbjct: 243 -FGWDRIVWGSDWPVCTLGGGLS 264 >SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 278 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 78/297 (26%), Positives = 124/297 (41%), Gaps = 34/297 (11%) Query: 24 IDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQGLHASITVQARQGRDETAF 83 ID H H + +YPW+ AG+ L +D L + + G+ AS+ ++ D Sbjct: 3 IDTHLHLIDKSALNYPWL-AGVPALDRDFLYATYAAEAKRVGVAASLHMEVDV--DPAEI 59 Query: 84 LLEMARRDKRIAG-----IIGWEDLSAPD---LAEQMERWGRHKLL-GLRHQLQEHDDAN 134 LE R R+AG + G P+ A +ER + + G R L D Sbjct: 60 ELE-TREVARLAGEPGSLLKGAIAACRPEDEGFAAYLERQEENAFVKGFRRVLHVVTDD- 117 Query: 135 TLLTPAPL-DQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPALKEF 193 L+ PL + V+ L +T D+ +P+ +L+H G P +K Sbjct: 118 --LSEQPLFRENVKRLSGTRFTFDLCVLPHQIPKAIALADLAPDVQFILDHCGVPDIKGH 175 Query: 194 RKGRAAFERWRQELRELTAMPHVACKISGLLT--EADWLRGLCTRDFEHIVQCLDTALDL 251 A WR + E+ P+V KISG++ E DW EH + + Sbjct: 176 -----AEHPWRDHMTEIARHPNVVAKISGVVAYAEEDWALDSIRPYVEHTIS-------V 223 Query: 252 FGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLC-PSDQDALWGESSARIYGLL 307 FG R+++GSDWPVC L + S V + + C P ++ L ++ RI+ L+ Sbjct: 224 FGWDRVVWGSDWPVCTLGGNLSTWVAATQ--ALIEGCSPQERRKLLSGNAQRIWNLI 278 >PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Pae Pseudomonas aeruginosa Length = 319 Score = 63.2 bits (152), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 73/301 (24%), Positives = 129/301 (42%), Gaps = 31/301 (10%) Query: 24 IDAHQHFWRYRSEDYPWIGAGMDL---------LAQDRLPYQFQPLLEAQGLHASITVQA 74 +DAH HFW ++ +PW+ + + + LP + + A + ++ V+ Sbjct: 10 VDAHHHFWDPQANYHPWLAEDAKIPFRYGDYSAIKRRYLPADYFGDVGAHRVVETVYVET 69 Query: 75 ----RQGRDETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQM-ERWGRHKLLGLRHQ--- 126 R ET F+ +A R ++ L APD AE + + G ++ +RH+ Sbjct: 70 EWDPRDPLGETRFVHHLAERHGAPHAVVAQAWLDAPDAAEVLAAQAGFARVRSVRHKPGG 129 Query: 127 ---LQEHDDANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLN 183 Q+ + +L++ +G L D+ +L + +VLN Sbjct: 130 PQRPQQVGELRSLMSDERWRRGYAELARHGLHFDLQTPWWNLAEAACLARDFPDTLIVLN 189 Query: 184 HLGKPALKEFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQ 243 H G P+ + +G AA W++ + P+VA KISG+ R D IV+ Sbjct: 190 HAGLPSDRS-EEGLAA---WQRAMARFAECPNVALKISGIGQAG---RRWSVEDNAWIVR 242 Query: 244 CLDTALDLFGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSARI 303 ++ LFG +R MF S++PV L S+ + + A L +DQ+ L+ ++ RI Sbjct: 243 ---ESIALFGVERAMFASNFPVDSLCGSFDDIYGGFKRIVAD-LPYADQERLFHSNARRI 298 Query: 304 Y 304 Y Sbjct: 299 Y 299