BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0141.p (309 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the T... 192 1e-48 AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the T... 124 3e-28 RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the T... 109 6e-24 Cgl0451 Non COG 83 9e-16 >YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Ype Yersinia pestis Length = 289 Score = 192 bits (487), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 110/278 (39%), Positives = 152/278 (54%), Gaps = 6/278 (2%) Query: 33 QPPRLKAPMLACDSHHHIYDAR--FPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQ 90 +P P ACDSH H+ R +P+ R P A Y ++A G++R V+VQ Sbjct: 16 EPSIFALPEDACDSHAHVISPRAIYPMVAE-RSYTPPPAPEEKYLSMLAATGMSRGVLVQ 74 Query: 91 PSTYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRVNFLSAQSWGV 150 S YGSDNR +L L + RG+ V+ +DV +L++M GVRG+R+N L G Sbjct: 75 ISVYGSDNRYMLQVLKRHPEYLRGVAVVTEDVTDQELQDMALAGVRGLRINVLFGGGIGF 134 Query: 151 TTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHP 210 +E L A +IAPLGWH+Q LM Q+ + LP VIDH+G +P GL+HP Sbjct: 135 DAMENL---ASKIAPLGWHMQFLMDVRQLPALIPRMKKLPCPCVIDHMGHMPVSLGLNHP 191 Query: 211 GAQAVLRLLAKGNTWIKLSEPYADTLQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPHPT 270 G Q +L ++ + W+KLS Y + Y D A+ ++ AP R +WGSDWPH + Sbjct: 192 GYQTLLHMVREYGWWVKLSGAYRISESWQDGYLDVVPFAQKLIETAPDRMVWGSDWPHVS 251 Query: 271 EKIKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGF 308 PD L +LLAAW PD +R +ILV NPA L+ F Sbjct: 252 VSRMPDTGGLRNLLAAWVPDEQVRNRILVDNPARLYDF 289 >AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 292 Score = 124 bits (311), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 75/240 (31%), Positives = 114/240 (47%), Gaps = 7/240 (2%) Query: 73 DYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDR 132 DYR LM LGI R ++ Q + + DN L + + G A +V+ID ++++ Sbjct: 52 DYRRLMQWLGIDRVIITQGNAHQRDNGNTLACVAEMGEAAHAVVIIDATTTEKDMEKLTA 111 Query: 133 LGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQ 192 G G R+ L G + L ER W V V G + H L + ++ Sbjct: 112 AGTVGARIMDLPG---GAVNLSELDAVDERAHAADWMVAVQFDGNGLLDHLPRLQKIRSR 168 Query: 193 VVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPPDYPDTSLLAKAY 252 V DH G+ + D P A+L+L+ +GN W K + Y + + P Y D + ++ Sbjct: 169 WVFDHHGKFFKGIRTDGPEMAALLKLIDRGNLWFKFAGVYESSRKSWP-YADVAAFSRVI 227 Query: 253 LQAAPQRTLWGSDWPHPTEK---IKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGFA 309 AP+R +WG++WPH + + PDDA L +L W PD A R + LV NP ALF + Sbjct: 228 AAHAPERIVWGTNWPHNSVRETAAYPDDARLAELTLGWLPDEAARHRALVENPEALFKLS 287 >RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Rso Ralstonia solanacearum Length = 302 Score = 109 bits (273), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 94/319 (29%), Positives = 139/319 (43%), Gaps = 27/319 (8%) Query: 1 MLDRRSFIAG--AMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVS 58 M RR+F G AML + + +P A + D+H H++ P++ Sbjct: 1 MPTRRAFNTGLLAMLGATALAGCVTRGMPAP--------TARITGIDTHAHVFKRGLPLA 52 Query: 59 PHWRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVI 118 R AT+ Y + G++ V++QPS G DN LL AL Q + RG+ VI Sbjct: 53 GARRYAPSYDATLPAYLAQLDAHGLSHGVLIQPSFLGGDNSYLLAALRQAPQRLRGVAVI 112 Query: 119 DDDVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTA-----ERIAPLGWHVQVL 173 D P L +++ G+ G+R+N + A +LR A R+ LGWHV++ Sbjct: 113 DPAAPEPFLAQLNAEGIVGIRLNLIGAPD------PQLRAPAWQAALHRLHALGWHVELH 166 Query: 174 MSGAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYA 233 ++ L VV+DH GR ++ PG A+L W+K+S Y Sbjct: 167 TQAHRLPALLPPLLEAQVDVVVDHFGRPDPALSVEDPGFAALLAAGRTRRVWVKVSGAYR 226 Query: 234 DTLQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPHP--TEKIKPDDAVLF-DLLAAWAPD 290 + G + + + + R +WGSDWPH +I D A F D L PD Sbjct: 227 NGDAGRGEAIALAAMPRLKDALGTDRLVWGSDWPHTQYESRIGYDSARAFADTL---LPD 283 Query: 291 PALRQQILVSNPAALFGFA 309 R Q+LV PA LF FA Sbjct: 284 AGDRHQVLVETPARLFRFA 302 >Cgl0451 Non COG Length = 250 Score = 82.8 bits (203), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 77/262 (29%), Positives = 121/262 (46%), Gaps = 21/262 (8%) Query: 45 DSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDA 104 DSH HI D + P+ + G P+ TV DY + L +A +V S D L DA Sbjct: 6 DSHFHIIDPQHPLIEN-NGYLPEPFTVEDYTARVEGLEVAGGAIVSGSFQAFDQGYLKDA 64 Query: 105 LTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIA 164 L G G+ I D ++ ++D+ GV+ VR+N S G+ +E L R +A Sbjct: 65 LAVLGPGYVGVTQIPADTSDQEILDLDKAGVKAVRLNLKRGGSAGLDDLETLARRVHDLA 124 Query: 165 PLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNT 224 GWH ++ + ++ + E+ LA+LP V IDHL GL G A+LRL+ G Sbjct: 125 --GWHTELYVDARELDELESTLASLPA-VSIDHL-------GLHRDGLPALLRLVENG-- 172 Query: 225 WIKLSEPYADTLQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLL 284 IK+ ++ P + + +A + P + G+D P K +DA L DL+ Sbjct: 173 -IKVKATGFGRVELDP-----TEVIQAIMAVDPTALMIGTDLPSTRTKRPFEDADL-DLI 225 Query: 285 AAWAPDPALRQQILVSNPAALF 306 A + + + +N AA + Sbjct: 226 AETVGEDHV-DNVFWNNAAAFY 246