BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0091.p (199 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO1525 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 84 2e-16 BMEII0061_1 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase co... 79 4e-15 RP261 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex,... 75 1e-13 SSO1369 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 73 3e-13 SP1164 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 73 5e-13 slr1934 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 71 1e-12 BH0776 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex... 71 2e-12 BS_acoA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 71 2e-12 CPn0304 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase comple... 70 2e-12 CT245 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex,... 67 3e-11 >SSO1525 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Sso Sulfolobus solfataricus Length = 332 Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/184 (31%), Positives = 88/184 (47%), Gaps = 15/184 (8%) Query: 1 MTKDELIAFEEEIAAT--FNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQ 58 M K L+ E+ A F +GKI VHLY G E + V +R D++ + R H Sbjct: 13 MYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGH 72 Query: 59 CLLKGVPPQAVREEILAGRSISLC-----------FPEQRIYSSAIVGGVLPIAVGTAMA 107 C+ KG+ + + EI+ G+ +C + + + ++ IVGG P AVG A+A Sbjct: 73 CIAKGLDVKRMLAEIM-GKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALA 131 Query: 108 IQRSGEDAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCTDTREAWN-Q 166 + G D F+G+ G+ ES+ S LP+ FVVEDN ++ T + Q Sbjct: 132 FKLKGLDRVAVAFIGDGAMNQGVVLESLNLSAIWKLPVIFVVEDNMYAMSTRSLVPGKLQ 191 Query: 167 PRLS 170 PR S Sbjct: 192 PRHS 195 >BMEII0061_1 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Bme Brucella melitensis Length = 401 Score = 79.3 bits (194), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 58/193 (30%), Positives = 89/193 (46%), Gaps = 30/193 (15%) Query: 8 AFEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLK----- 62 AFEE++ G + P H G E + +RP D + S R+H+Q L K Sbjct: 43 AFEEKVLELAGQGLVHGPAHSAIGQEGGAVGSAVSMRPSDQINGSHRAHHQFLAKALGYV 102 Query: 63 ---GVPPQAVRE------------EILA---------GRSISLCFPEQ-RIYSSAIVGGV 97 G+ P+A + EIL G S+ L + E + ++AIVGG Sbjct: 103 AQKGIDPKAAFDQDIRTLAQRTLAEILGLSQGFCRGRGGSMHLRWAESGNLGTNAIVGGG 162 Query: 98 LPIAVGTAMAIQRSGEDAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVC 157 +P+A G A A +R+G+ VY + G+ + G E++ + LPI F +E+N +V Sbjct: 163 VPMAAGAAWAHRRAGKGDVVYTYFGDGATNIGSVLETMNLAAAWKLPICFFIENNRYAVS 222 Query: 158 TDTREAWNQPRLS 170 T E +PRLS Sbjct: 223 THVEEVTAEPRLS 235 >RP261 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Rpr Rickettsia prowazekii Length = 326 Score = 74.7 bits (182), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 51/168 (30%), Positives = 77/168 (45%), Gaps = 12/168 (7%) Query: 3 KDELIA--FEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCL 60 KD L+ FEE+ + GKI HLY G E I V + D S+R H + Sbjct: 20 KDMLLLRRFEEKCGQLYGMGKIGGFCHLYIGQEAVISAVAMIKKKGDSTITSYRDHAHII 79 Query: 61 LKGVPPQAVREEILA---------GRSISLCFPEQRIYSS-AIVGGVLPIAVGTAMAIQR 110 L G P+ V E++ G S+ L + Y IVG +PI G A A + Sbjct: 80 LAGTEPKYVLAELMGRATGCSKGKGGSMHLFDIPNKFYGGHGIVGAQVPIGTGLAFAEKY 139 Query: 111 SGEDAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCT 158 +G + + F+G+ G +E+ + GLPI +++E+NE S+ T Sbjct: 140 NGTNNICFTFLGDGAVNQGQVYEAFNMASLWGLPIVYIIENNEYSMGT 187 >SSO1369 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Sso Sulfolobus solfataricus Length = 345 Score = 73.2 bits (178), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 46/150 (30%), Positives = 72/150 (48%), Gaps = 11/150 (7%) Query: 19 AGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKGVPPQAVREEILAGRS 78 +G+IR +HL G E + IR +D V + R H+ + KGV + + EIL G++ Sbjct: 58 SGRIRGEMHLSIGQEAVAVGTLYDIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEIL-GKA 116 Query: 79 ISLC---------FPEQRIYS-SAIVGGVLPIAVGTAMAIQRSGEDAKVYCFMGEMTSET 128 LC F + + ++ S IVG P A G A A + G+D F GE + Sbjct: 117 TGLCKGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLGKDNVAISFAGEGAANH 176 Query: 129 GIAHESIKYSRNHGLPIHFVVEDNEKSVCT 158 G E++ + LP+ V+EDN+ + T Sbjct: 177 GTFAETLNIASAWELPLIIVIEDNKYADST 206 >SP1164 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Spn Streptococcus pneumoniae TIGR4 Length = 322 Score = 72.8 bits (177), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 50/213 (23%), Positives = 88/213 (41%), Gaps = 22/213 (10%) Query: 4 DELIAFEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKG 63 +E+ + +IA GK+ H G E + + P D + + R H Q + KG Sbjct: 17 EEIRRMDLKIAQLVKKGKVPGMTHFSVGEEAANVGAMLALNPDDLITSNHRGHGQAIAKG 76 Query: 64 VPPQAVREEILA-------GRSISLCFPE---QRIYSSAIVGGVLPIAVGTAMAIQRSGE 113 + + EIL G+ S+ + + ++ IVGG + IAVG A++ Q Sbjct: 77 IDLNGMMAEILGKYTGTCKGKGGSMHIADLDAGNLGANGIVGGGMGIAVGAALSQQMQNT 136 Query: 114 DAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCTDTRE----------- 162 V CF G+ + G+ HE++ + LP+ F +N + D ++ Sbjct: 137 GKIVVCFFGDGATNEGVFHEAVNMASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRS 196 Query: 163 -AWNQPRLSYEEVNDDFITYYRYQTKYPHAGAG 194 A+ P + E+ N+ Y +Q H +G Sbjct: 197 AAYGIPGMFIEDGNNVIDVYEGFQKAVDHVRSG 229 >slr1934 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Syn Synechocystis Length = 342 Score = 71.2 bits (173), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 54/181 (29%), Positives = 81/181 (44%), Gaps = 22/181 (12%) Query: 9 FEEEIAATFNAGKIRAPVHLYYGNE---EQIIDVFRQIRPQDWVFCSWRSHYQCLLKGVP 65 FE++ A + GK+ VHLY G E II RQ +D+V ++R H L GVP Sbjct: 35 FEDKCAEMYYRGKMFGFVHLYNGQEAVSSGIIKAMRQ--DEDYVCSTYRDHVHALSAGVP 92 Query: 66 PQAVREEILA---------GRSISLCFPEQRIYSS-AIVGGVLPIAVGTAM-------AI 108 + V E+ G S+ L + A +G +P+A+G A + Sbjct: 93 AREVMAELFGKETGCSRGRGGSMHLFSSAHNLLGGFAFIGEGIPVALGAAFQTKYRREVL 152 Query: 109 QRSGEDAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCTDTREAWNQPR 168 + G D CF G+ TS G E + + LPI FVVE+N+ ++ A +QP Sbjct: 153 KDDGYDQVTACFFGDGTSNNGQFFECLNMAALWKLPILFVVENNKWAIGMAHERATSQPE 212 Query: 169 L 169 + Sbjct: 213 I 213 >BH0776 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Bha Bacillus halodurans Length = 326 Score = 70.9 bits (172), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 45/173 (26%), Positives = 71/173 (41%), Gaps = 12/173 (6%) Query: 9 FEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKGVPPQA 68 FEE++ F G I HL G E + + +D + + R H C+ KG Sbjct: 28 FEEKVDEFFAKGMIHGTTHLAVGQEASAVGSIAVLEERDKLTSTHRGHGHCIAKGADVNR 87 Query: 69 VREEILAGRSISLC-----------FPEQRIYSSAIVGGVLPIAVGTAMAIQRSGEDAKV 117 + E+ GR C + ++ IVGG IA G A+ + E V Sbjct: 88 MMAELF-GRETGYCKGKGGSMHIADVERGNLGANGIVGGGFSIATGAALTSKMKKEGYVV 146 Query: 118 YCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCTDTREAWNQPRLS 170 CF G+ S G HE++ + LP+ F+ E+N+ + +E N +S Sbjct: 147 LCFFGDGASNEGSFHEAVNLASIWKLPVVFICENNQYGMSGSVKEMINIEHIS 199 >BS_acoA [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Bsu Bacillus subtilis Length = 333 Score = 70.9 bits (172), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 50/202 (24%), Positives = 83/202 (41%), Gaps = 26/202 (12%) Query: 5 ELIAFEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKGV 64 E+ FE+++ F G + VHLY G E + V + D + + R H C+ KG Sbjct: 25 EIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLHDGDSITSTHRGHGHCIAKGC 84 Query: 65 PPQAVREEILAGRSISLC-----------FPEQRIYSSAIVGGVLPIAVGTAMAIQRSGE 113 + EI G++ LC + + ++ IVGG +A G+A+ + Sbjct: 85 DLDGMMAEIF-GKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFTLACGSALTAKYKQT 143 Query: 114 DAKVYCFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNE---------KSVC---TDTR 161 CF G+ + G HE + + LP+ FV E+N S C D Sbjct: 144 KNVSVCFFGDGANNQGTFHEGLNLAAVWNLPVVFVAENNGYGEATPFEYASACDSIADRA 203 Query: 162 EAWNQPRLSYEEVNDDFITYYR 183 A+N P ++ + D + Y+ Sbjct: 204 AAYNMPGVTVD--GKDILAVYQ 223 >CPn0304 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Cpn Chlamydophila pneumoniae CWL029 Length = 342 Score = 70.5 bits (171), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 48/170 (28%), Positives = 73/170 (42%), Gaps = 12/170 (7%) Query: 9 FEEEIAATFNAGKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKGVPPQA 68 FE + G + H Y G E WVF S+R H +L +P Q Sbjct: 44 FEARGEEAYLEGLVGGFYHSYAGQEAVATAAIANTGLDPWVFSSYRCHALAILLNIPLQE 103 Query: 69 VREEILA---------GRSISLCFPEQRIYSSAIVGGVLPIAVGTAMAIQRSGEDAKV-Y 118 + E+L G S+ +C P IVGG +P+A G A I+ + +V Sbjct: 104 IAAELLGKETGCALGRGGSMHMCGP-NFPGGFGIVGGQIPLAAGAAFTIKYQEQKNRVSL 162 Query: 119 CFMGEMTSETGIAHESIKYSRNHGLPIHFVVEDNEKSVCTD-TREAWNQP 167 CF+G+ G+ HE++ + H LP+ ++E+N S+ T R QP Sbjct: 163 CFIGDGAVAQGVFHETLNFVSLHQLPLMLIIENNGWSMGTSLNRAVAKQP 212 >CT245 [C] COG1071 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit Ctr Chlamydia trachomatis Length = 340 Score = 66.6 bits (161), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 41/152 (26%), Positives = 66/152 (43%), Gaps = 8/152 (5%) Query: 20 GKIRAPVHLYYGNEEQIIDVFRQIRPQDWVFCSWRSHYQCLLKGVPPQAVREEILA---- 75 G + H Y G E W F S+R H LL +P + + E+L Sbjct: 54 GLVGGFYHSYIGQEAVATAAIACTGKDHWFFSSYRCHGVALLLDIPLRQLAAELLGKETG 113 Query: 76 ---GRSISLCFPEQRIYSS-AIVGGVLPIAVGTAMAIQRSGEDAKVYCFMGEMTSETGIA 131 GR S+ R+ IVGG +P+A G A +++ + CF+G+ G+ Sbjct: 114 CALGRGGSMHMCGDRLPGGFGIVGGQIPLAAGAAFSMKYQNSSSISMCFIGDGAVAQGVF 173 Query: 132 HESIKYSRNHGLPIHFVVEDNEKSVCTDTREA 163 HE++ + H LP+ ++E+N S+ T A Sbjct: 174 HETLNFVALHSLPLMLIIENNGWSMGTALHRA 205