BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0021.p (677 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1286 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 427 e-119 PA3179 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 266 9e-71 STM1719 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 265 3e-70 ZyciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase ... 264 5e-70 ECs1841 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 264 5e-70 yciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase a... 264 5e-70 YPO2213 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 263 1e-69 HI1199 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 261 3e-69 PM0661 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 261 4e-69 NMB1361 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 259 1e-68 >RSc1286 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Rso Ralstonia solanacearum Length = 598 Score = 427 bits (1098), Expect = e-119, Method: Compositional matrix adjust. Identities = 204/285 (71%), Positives = 232/285 (81%), Gaps = 7/285 (2%) Query: 269 DDIFSYVTSEAYDRDENPKGGARQPHQMRGKNGRRDLTAEDDAPKLHKVLADAGLGSRRD 328 +D+F YV S AYD D + GGA Q ++R +LTAEDDAPKLHK+LADAGLGSRRD Sbjct: 178 EDVFQYVISGAYDADAD-VGGAPQSTKVR------ELTAEDDAPKLHKILADAGLGSRRD 230 Query: 329 MEELIVAGRVSVNGEPAHIGQRILPTDAVRINGKLIQRKVSKRPPRVLVYHKPAGEIVSH 388 ME+LI+ GRVSVNG PAHIGQRILPTD VR+NGKLIQRK+ +PPRVL+YHKPAGEIVS Sbjct: 231 MEDLILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQ 290 Query: 389 ADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSGDLANRLMHPRYNIEREYAV 448 +DPEGR +VFD LP MK GKW+AVGRLD+NTEGLL+FTTSGD+ANR MHPRY +EREYAV Sbjct: 291 SDPEGRPTVFDSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAV 350 Query: 449 RTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVNKWYRVTIGEGRNREVRRMFEA 508 RTLGEL E RQKLL G+ LDDG A F R ADGGGEGVN WY V + EGRNREVRRMFEA Sbjct: 351 RTLGELPESDRQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGRNREVRRMFEA 410 Query: 509 VGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAACGLE 553 V LTVSRLIRTRYG+ LP+ L+RGRW+E+ VR LM GL+ Sbjct: 411 VNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLK 455 >PA3179 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Pae Pseudomonas aeruginosa Length = 386 Score = 266 bits (681), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 132/251 (52%), Positives = 176/251 (70%), Gaps = 2/251 (0%) Query: 313 KLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDAVRINGKLIQRK-VSKR 371 KL KVLA G+GSRRD+E I GRV+VNG A +GQR+ DA+ ++G LI+R+ ++ Sbjct: 15 KLQKVLARLGVGSRRDVEVWIAEGRVNVNGSVASLGQRVDSHDAITVDGHLIRREEAAES 74 Query: 372 PPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSGDL 431 RVL+Y+KP GE+ + DPEGR ++FDRLP ++ G+W+ VGRLD NT GLLLFTT G+L Sbjct: 75 VRRVLIYNKPEGEVCTRDDPEGRPTIFDRLPRLRTGRWINVGRLDINTTGLLLFTTDGEL 134 Query: 432 ANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADG-GGEGVNKWY 490 ANRLMHP Y ++REYAVR GE+ E M ++LL+GV L+DGPA+FS I GGEG N WY Sbjct: 135 ANRLMHPSYEMDREYAVRVRGEVTEEMIERLLNGVMLEDGPAKFSDIQQAPGGEGFNHWY 194 Query: 491 RVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAAC 550 + EGRNREVRR++E+ GL VSRL R R+G + L L GR+ E+++ + L Sbjct: 195 HCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLTSELTMGRYREMDQREIDILSEEV 254 Query: 551 GLEKQAGEGKS 561 GL+ A G + Sbjct: 255 GLKPVALPGMT 265 >STM1719 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Sty Salmonella typhimurium LT2 Length = 291 Score = 265 bits (676), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 137/243 (56%), Positives = 176/243 (72%), Gaps = 6/243 (2%) Query: 311 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQRKV 368 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI K Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIATLGDRVEVTPGLKIRIDGHLISVKE 61 Query: 369 S-KRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 427 S ++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 428 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 487 G+LANRLMHP +EREYAVR G+++E + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDESKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 488 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 544 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 545 NLM 547 L+ Sbjct: 242 GLV 244 >ZyciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases EcZ Escherichia coli O157:H7 EDL933 Length = 290 Score = 264 bits (675), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 135/243 (55%), Positives = 177/243 (72%), Gaps = 6/243 (2%) Query: 311 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQ-RK 367 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI R+ Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRE 61 Query: 368 VSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 427 +++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 428 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 487 G+LANRLMHP +EREYAVR G++++ + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 488 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 544 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 545 NLM 547 L+ Sbjct: 242 ELV 244 >ECs1841 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Ecs Escherichia coli O157:H7 Length = 290 Score = 264 bits (675), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 135/243 (55%), Positives = 177/243 (72%), Gaps = 6/243 (2%) Query: 311 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQ-RK 367 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI R+ Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRE 61 Query: 368 VSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 427 +++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 428 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 487 G+LANRLMHP +EREYAVR G++++ + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 488 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 544 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 545 NLM 547 L+ Sbjct: 242 ELV 244 >yciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Eco Escherichia coli K12 Length = 291 Score = 264 bits (675), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 135/243 (55%), Positives = 177/243 (72%), Gaps = 6/243 (2%) Query: 311 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQ-RK 367 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI R+ Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRE 61 Query: 368 VSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 427 +++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 428 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 487 G+LANRLMHP +EREYAVR G++++ + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 488 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 544 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 545 NLM 547 L+ Sbjct: 242 ELV 244 >YPO2213 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Ype Yersinia pestis Length = 318 Score = 263 bits (671), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 131/247 (53%), Positives = 176/247 (71%), Gaps = 3/247 (1%) Query: 313 KLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDA--VRINGKLIQRKVSK 370 KL K+LA AG GSRR++E +I GRVSV+G+ + +G R+ T + +R++G L+ K S+ Sbjct: 4 KLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIKESE 63 Query: 371 R-PPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSG 429 RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT G Sbjct: 64 ENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDG 123 Query: 430 DLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVNKW 489 +LANRLMHP +EREYAVR G++++ ++L GV+L+DGPA F I+ GGEG+N+W Sbjct: 124 ELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGINQW 183 Query: 490 YRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAA 549 Y VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL+ A L Sbjct: 184 YNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYLREL 243 Query: 550 CGLEKQA 556 L+ + Sbjct: 244 VELDVET 250 >HI1199 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Hin Haemophilus influenzae Length = 357 Score = 261 bits (668), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 131/243 (53%), Positives = 169/243 (69%), Gaps = 3/243 (1%) Query: 313 KLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTD--AVRINGKLIQ-RKVS 369 KL KVLA AG GSRR++E +I AGRVSV G+ A +G RI VRI+G++I Sbjct: 77 KLQKVLARAGQGSRREIETMIAAGRVSVEGKIATLGDRIDVHSGVKVRIDGQIINLSHTQ 136 Query: 370 KRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSG 429 K RVL+Y+KP GE+ + +DPEGR +VFDRLP + +W+AVGRLD NT GLLLFTT G Sbjct: 137 KEICRVLMYYKPEGELCTRSDPEGRATVFDRLPRLTGSRWIAVGRLDINTSGLLLFTTDG 196 Query: 430 DLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVNKW 489 +LANRLMHP +EREY+VR G++++ M +L GV+L+DG A F I GG G+N+W Sbjct: 197 ELANRLMHPSREVEREYSVRVFGQVDDAMLARLRKGVQLEDGLANFKEIKFTGGVGINQW 256 Query: 490 YRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAA 549 Y VT+ EGRNREVRR++E+ G+ VSRLIR RYG + L + L RG WEE++ V L Sbjct: 257 YDVTLMEGRNREVRRLWESQGIQVSRLIRIRYGNIKLMKGLPRGGWEEMDLENVNYLREL 316 Query: 550 CGL 552 GL Sbjct: 317 VGL 319 >PM0661 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Pmu Pasteurella multocida Length = 345 Score = 261 bits (667), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 132/246 (53%), Positives = 171/246 (69%), Gaps = 9/246 (3%) Query: 313 KLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDA-----VRINGKLIQR- 366 KL KVLA AG GSRR++E +I RVSV+G+ A +G RI D +RI+G LI Sbjct: 65 KLQKVLARAGQGSRREIEAMIAENRVSVDGKIATLGDRI---DVHAGVKIRIDGHLINLL 121 Query: 367 KVSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFT 426 K RVL+Y+KP GE+ + DPEGR +VFDRLP + +W+AVGRLD NT GLLLFT Sbjct: 122 HAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLTGSRWIAVGRLDINTSGLLLFT 181 Query: 427 TSGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGV 486 T G+LANRLMHP +EREY+VR G+++E M +L GV+L+DGPA F I GG G+ Sbjct: 182 TDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVGM 241 Query: 487 NKWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNL 546 N+W+ VT+ EGRNREVRR++E+ G+ VSRLIRTRYG ++L ++L RG WEE++ V L Sbjct: 242 NQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNYL 301 Query: 547 MAACGL 552 GL Sbjct: 302 RELVGL 307 >NMB1361 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Nme Neisseria meningitidis MC58 Length = 350 Score = 259 bits (662), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 127/252 (50%), Positives = 171/252 (67%), Gaps = 1/252 (0%) Query: 302 RRDLTAEDDAPKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDAVRING 361 RR + + +L KVLA +G+GSRR+MEE I G ++VNG+ A +G ++ P D V + G Sbjct: 88 RRSDLSRMEPERLQKVLAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKG 147 Query: 362 KLIQRKVSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEG 421 +I+ K + R PR+++Y+K GEIVS DP+GR S+FDRLP + +W+A+GRLD NT G Sbjct: 148 SIIKLKWADRLPRIILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSG 207 Query: 422 LLLFTTSGDLANRLMHPRYNIEREYAVRTLGELE-EGMRQKLLSGVELDDGPAQFSRIAD 480 LL+ TTSG+L R HP + +EREYAVR LG L E MR GV L+DG A+ RI + Sbjct: 208 LLILTTSGELVQRFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIRE 267 Query: 481 GGGEGVNKWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEE 540 GGEG NKWY V I EGRNREVRR+FE+ GLTVSRL+R +G + LP L+RG++ EL Sbjct: 268 QGGEGANKWYNVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNP 327 Query: 541 NAVRNLMAACGL 552 V N++ + Sbjct: 328 AEVANIIKWADM 339